Streptococcus suis and haemophilus parasuis bivalent inactivated vaccine and preparation method thereof

Abstract

The invention discloses a streptococcus suis and haemophilus parasuis combined inactivated vaccine and a preparation method thereof. The streptococcus suis and haemophilus parasuis combined inactivated vaccine comprises an antigen and an adjuvant, the antigen comprises an inactivated antigen concentrated solution of a haemophilus parasuis serum type 4 YC strain, a haemophilus parasuis serum type 5 SQ strain, a streptococcus suis type 2 NT strain and a streptococcus suis type 9 CZ strain. The vaccine provided by the invention can be used for simultaneously preventing haemophilus parasuis disease caused by haemophilus parasuis, streptococcus suis disease caused by streptococcus suis and mixed infection of the haemophilus parasuis disease and the streptococcus suis disease, is small in side effect and good in safety, can achieve the effect of preventing multiple diseases by one injection, and particularly can be used for preventing and treating infection of currently popular serotype germs; comprising infection of haemophilus parasuis type 4 and type 5 and infection of streptococcus suis type 2 and type 9.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to a dual inactivated vaccine of Streptococcus suis and Haemophilus parasuis and a preparation method thereof. Background technique [0002] There are 33 serotypes of Streptococcus suis, among which Streptococcus suis type 2 is the most prevalent and has the highest incidence rate, and can infect people to death. Sporadic or local outbreaks, morbidity and mortality are all very high, seriously endangering the development of the pig industry, so far, the research of Streptococcus suis in various countries has always been a hot spot of pig diseases, especially the research on SS2 has been from It has not stopped. Although type 2 is the most widely distributed and most virulent among all serotypes of Streptococcus suis, in recent years, the isolation rate and pathogenicity rate of SS9 in China have a clear upward trend. Become the main epidemic strain, Haemophilus parasuis disease, a...

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Problems solved by technology

[0002] There are 33 serotypes of Streptococcus suis, among which Streptococcus suis type 2 is the most prevalent and has the highest incidence rate, and can infect people to death. Sporadic or local outbreaks, morbidity and mortality are all very high, seriously endangering the development of the pig industry, so far, the research of Streptococcus suis in various countries has always been a hot spot of pig diseases, especially the research on SS2 has been from It has not stopped. Although type 2 is the most widely distributed and most virulent among all serotypes of Streptococcus suis, in recent years, the isolation rate and pathogenicity rate of SS9 in China have a clear upward trend. Become the main epidemic strain, Haemophilus parasuis disease, also known as "Glasser's disease", can cause polyserositis and arthritis in pigs, with fibrinous polyserositis, arthritis and meningitis It is characterized by HPS, which has caused serious economic losses to the global pig industry. Haemophilus parasuis mainly infects weaned piglets aged 4-12 weeks. HPS was isolated for the first time in 1922, causing great harm to the pig industry, especially It is a mixed infection with some immunosuppressive diseases, such as porcine reproductive and respiratory syndrome, circovirus disease, etc., which is more harmful. In China, serotypes 4 and 5 are the most prevalent, followed by serotypes 13, 14 and 12 , while serotypes 1, 2, 6, 7, 9, 10, 11, and 15 are represented by only a few strains, and another 12.1% cannot be typed. With the standardized use of antibiotics, Haemophilus parasuis and chain of suis The prevention and control of coccus gradually relies on vaccines. Currently, the domestic Streptococcus suis vaccines that have been registered and produced include live Streptococcus suis vaccine, inactivated Streptococcus suis vaccine, and subunit vaccine of Streptococcus suis. Some live vaccines include RD strain live vaccine, inactivated There are 6 types of vaccines, including monovalent inactivated vaccines, multivalent inactivated vaccines and multivalent inactivated vaccines. Among them, the strains include SS2, SS7 and Streptococcus equi subspecies zooepidemicus. The domestic Haemophilus parasuis vaccine is multivalent inactivated Live vaccines are mainly live vaccines, including the bivalent inactivated vaccine for Haemophilus parasuis jointly developed by Shandong Academy of Agricultural Sciences Animal Husbandry and Veterinary Research Institute, Shandong Binzhou Wohua Bioengineering Co., Ltd., and Pulaike Bioengineering Co., Ltd. Bivalent inactivated vaccine for Haemophilus parasuis; Quadrivalent propolis inactivated vaccine for Haemophilus parasuis jointly developed by Shandong Huahong Bioengineering Co., Ltd. and Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Wuhan Keqian Biotechnology Co., Ltd. The research and development of Streptococcus suis and Haemophilus parasuis dual inactivated vaccines. In recent years, the domestic Haemophilus parasuis inactivated vaccines are mainly types 4 and 5, which is similar to the prevalence of parasuis in pig farms. strain related

[0003] Chinese invention patent application CN102329746A discloses a dual live vaccine of Streptococcus suis and Haemophilus parasuis and its preparation method. The vaccine is obtained by multiplying and culturing Streptococcus suis and Haemophilus parasuis Bacterial liquid, after inactivation, mix, add Tween to make water phase, mix water phase and oil phase 4:6, make oil adjuvant inactivated vaccine, oil adjuvant side reaction is relatively large, it will cause inflammation in the vaccine injection department or swelling, and the viscosity of the oil adjuvant is relatively high, so it is difficult to extract and inject during injection;

[0004] Chinese invention patent application CN103157101A discloses a double inactivated vaccine of Haemophilus parasuis and Streptococcus suis and its preparation method. The vaccine consists of inactivated Haemophilus parasuis serum type 4 Js strain and 5 type zJ strain, Streptococcus suis type 2 R group SC strain and Streptococcus suis group C Streptococcus equi subsp. Streptococcus suis type 9 has no cross-protective effect, while streptococcus type 2 and type 9 have a high isolation rate in diseased pigs. Streptococcus suis caused by Haemophilus and Streptococcus suis and their mixed infection

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