54 results about "Streptococcus equi" patented technology

Disclosed are DNA segments encoding <DEL-S DATE="20010821" ID="DEL-S-00001">hyaluronic acid synthase which are employed to construct recombinant cells useful in the production of hyaluronate synthase or hyaluronic acid (HA)<DEL-E ID="DEL-S-00001"> <INS-S DATE="20010821" ID="INS-S-00001">the recombinant DNA segment identified in FIG. 5. <INS-E ID="INS-S-00001">In preferred aspects, chromosomal DNA from Streptococcus equisimilis is partially digested with EcoRI and the resultant fragments are ligated to form recombinant vectors. These vectors are useful in the transformation of host cells such as E. coli and or Streptococcal hosts. <DEL-S DATE="20010821" ID="DEL-S-00002">Resultant transformants are screened by the novel screening assays to identify colonies which have incorporated HA synthase DNA in a form that is being actively transcribed into the corresponding HA synthase enzyme. These colonies may be selected and employed in the production of the enzyme itself or its product, HA.<DEL-E ID="DEL-S-00002"> <INS-S DATE="20010821" ID="INS-S-00002">The recombinant DNA segment identified in FIG. 5 is then inserted into a recombinant Streptococcal host for the production of hyaluronic acid (HA).<INS-E ID="INS-S-00002">

The invention relates generally to methods and materials concerning diseases caused by Streptococcus equi, and in particular relating to the detection of this pathogen by assessing the presence or absence of the S. equi eqbE gene sequence.

The invention discloses a Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and an application in the fermentation production of hyaluronic acid. The SXY36 strains of Streptococcus equi subsp. Zooepidemicus subspecies and a method for controlling HA molecular weight using the fermentation process are provided. The method has the advantages that SXY36 is subjected to irradiation mutation breeding by ultraviolet rays, microwave and gamma rays, no hemolysis exists, the HA fermentation yield is high, and the heredity is stable, in the optimal fermentation condition, the fermentation yield of shaking flask is up to 0.731 g/L, and the yield of fermentation by a fermentation cylinder can reach 4.78 g/L; a two-stage variable temperature fermentation and stable pH method is adopted, high molecular-weight HA can be prepared by fermentation, and the molecular weight can reach 1.89 MDa, which is two times the molecular weight by a conventional fermentation method; HA with the molecular weight in the range of 0.035-1.89 MDa can be produced by using a fermentation broth self-enzymatic degradation method to prepare different molecular-weight HA and by controlling the time of enzymatic hydrolysis.

The invention relates to a production technology for fermentatively producing sodium hyaluronate by utilizing bacterium. The production technology comprises the following steps of: using streptococcus equi subsp. zooepidemicus (Streptococcus Zooepidemicus) as an original strain; mutagenizing by virtue of nitrosoguanidine, and also mutagenizing by virtue of a protoplast in the presence of ultraviolet rays; introducing a special culture medium; carrying out submerged fermentation to produce high-output hyaluronic acid; and then carrying out a relative separation and purification technology to obtain a high-purity sodium hyaluronate product. According to the production technology for fermentatively producing the sodium hyaluronate by utilizing the bacterium, under the culture conditions that the culture temperature is 32 to 38 DEG C, the pH (Potential Of Hydrogen) of fermentation liquor is maintained within the range of 5.0 to 9.0, and a fermentation tank is at speed of stirring of 150 to 600rev/min, the output of the hyaluronic acid is greatly increased; and the production technology is suitable for being applied to industrial production.

The invention relates to subunit immunogenic or vaccine compositions which may comprise at least one polypeptide of Streptococcus equi and methods for preparing and/or formulating such compositions. The invention also relates to the use of such subunit compositions, such as a method for eliciting an immunogenic response or a protective immune response, which may comprise administering the composition to a mammal susceptible to streptococcal infection.

The invention belongs to the technical field of preparation of animal vaccines and particularly relates to a trivalent inactivated vaccine for Streptococcus equi subsp. zooepidemicus, Streptococcus suis serotype 2 and Streptococcus suis serotype 7, as well as a preparation method and application thereof. The inactivated vaccine disclosed by the invention is mainly applicable to prevention and treatment of swine Streptococcosis for sows, boars and piglets. The invention discloses separation, identification and application of three strains special for preparing the swine Streptococcosis trivalent inactivated vaccine, wherein the three strains for the vaccine are respectively a Streptococcus suis serotype 2-LT strain (with the collection number being CCTCC NO:M2011282), a Streptococcus suis serotype 7-YZ strain (with the collection number being CCTCC NO:M2011160) and a Streptococcus equi subsp. Zooepidemicus XS strain (with the collection number being CCTCC NO:M2011405). The inactivated vaccine disclosed by the invention can achieve multiple prevention effects with one injection, so that the stresses of swine are reduced, and the inactivated vaccine is safe and reliable and is free from the potential hazards of toxin spreading.

The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.

The invention discloses serum-free anaerobic high-density fermentation culture process for Streptococcus equi subsp. Zooepidemicu and belongs to the field of agricultural microbiology. Streptococcus equi subsp. Zooepidemicu fermented high-density antigens are obtained through the sequential steps of resuscitating lyophilized seeds with TSA, carrying out primary culture on a slant of TS agar, carrying out secondary seed culture on a TSB medium, and anaerobically fermenting in enriched trypsin-casein medium free of serum and glucose. Study on industrial enlarged production is carried out through the method of anaerobic fermentation culture without adding any serum or glucose, the content of fermented antigens is not reduced, three is no need for ventilation regulation and dissolved oxygen control during fermentation, the antigen content is high, process parameters are easy to control, and supplements are reduced; the process is stable and high in growth speed compared with the existing fermentation process and is easy for implementing large-scale high-density culture of Streptococcus equinus antigens; immunogenicity of a strain is improved, and protection for vaccines is improved.

The invention discloses a streptococcus equi subsp and a production technology for preparing hyaluronic acid through the streptococcus equi subsp. According to the novel screened streptococcus equi subsp, the improved fermentation technology is combined, the output rate of a hyaluronic acid product is high, and can be 9.5 g/L. The fermented streptococcus equi subsp is easy to separate and purify, and the production cycle is short; production is easy to control, the streptococcus equi subsp is suitable for industrial mass production, the yield is high, and the production cost is greatly reduced.

This invention discloses a lysin that can kill many species of Streptococci. A new lysin, ClyR, was constructed by the gene splicing method. The ClyR can effectively kill different species of Streptococci, including Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus suis, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus mutans, Streptococcus equi, and various Enterococci and Staphylococcus aureus. ClyR shows good stability and is not sensitive to EDTA and high concentration of NaCl. Moreover, the ClyR is active in a wide range of pH and maintains high activity in pH 5-11. Recombinant protein ClyR is well expressed in E. coli stain BL21 (DE3). High doses of ClyR showed no apparent toxicity in mice. Furthermore, administration of 0.8 mg per mouse once is able to completely protect the mouse infected with lethal doses of Group B Streptococci. The ClyR can be used alone or in combination with different forms of reagents and solutions, for the control of a variety of Streptococci and for the treatment of infections caused by these bacteria. It has a broad application prospect.

The invention provides lyase capable of killing streptococcus equi subspecies. Equi and medical application, and provides a new bacteriophage lyase which can be independently used or compounded with other substances for use, has strong lytic activity and a wide lytic spectrum, can effectively kill streptococcus equi subsp. Equi and streptococcus suis. The invention provides a novel medicine for preventing and treating diseases caused by streptococcus equi subsp. Equi and streptococcus suis infection.

This invention relates to compositions comprising live, attenuated Streptococcus equi (S. equi), or a fractional extract of S. equi, in combination with at least one immunostimulant for stimulating mucosal immunity, such as saponin. The invention also relates to methods of preparation and dosage forms containing the composition of the invention as well as methods of use for stimulating the immune system of an equine and inducing an immune response to S. equi by contacting the cells of nasopharyngeal mucosa with the composition of the invention. Furthermore, the invention relates to a method of immunizing an equine to induce protective immunity against S. equi.

The invention discloses a donkey-derived streptococcus equi subsp. Equi HLJ2018D-LX strain and application thereof in preparation of a streptococcus equi subsp. Equi inactivated vaccine, and belongs to the field of biological vaccines. The donkey-derived streptococcus equi subsp. Equi HLJ2018D-LX strain has the advantages of good immunogenicity, good culture characteristics and the like, and the inactivated vaccine is prepared by inoculating the streptococcus equi subsp. Equi HLJ2018D-LX strain into a streptococcus enrichment liquid, performing culturing, and performing inactivating with formaldehyde (the final concentration is 0.2% v/v). Experimental proves that the streptococcus equi subsp. Equi inactivated vaccine prepared by the invention is used for immunizing mice, the streptococcusequi subsp. Equi inactivated vaccine can prevent mice from being attacked by virulent streptococcus equi subsp. Equi, and greatly reduces the morbidity in donkey body tests. The vaccine has the advantages of safety, low-dose immunization and the like, is the first streptococcus equi subsp. Equi inactivated vaccine for donkeys, and can be used for preventing strangles caused by streptococcus equi subsp. Equi.

The invention belongs to the field of eqidemiology and sanitation detection, and discloses an ELISA (enzyme-linked immuno sorbent assay) quick detection kit and a method both for quickly detecting streptococcus equi subsp. zooepidemicus; particularly, the kit provided by the invention includes a recombined streptococcus equi subsp. zooepidemicus type M protein antigen and an ELIAS secondary antibody. The invention also discloses an indirect ELISA method for detecting streptococcus equi subsp. zooepidemicus. The method is quick, simple and convenient, and can be used for directly detecting to-be-detected blood serum. The detection kit and the detection method provided by the invention have high sensitiveness and strong specificity.

An isolated peptide fragment of the VapA protein that binds antibodies specific for Rhodococcus equi and the VapA protein. In a preferred form the peptide contains an amino acid sequence of 5 or more amino acid residues that is identical to or homologous to the amino acid sequence of at least one region of the VapA protein that is responsible for immunological recognition. Methods of diagnosing a vertebrate for the presence of R. equi using the peptide and methods of vaccinating a vertebrate against R. equi using the peptide are also claimed.

The present invention relates to a PCR detection kit for detecting Streptococcus equi (further called Streptococcus equi subsp equi). The detection kit comprises primers, dNTP, a buffer and DNA polymerase, wherein the upstream primer is 5'-CTATTAAAGTCTCCATTG-3', and the downstream primer is 5'-AATGTTGTTCAAGCAAATTC-3'. The PCR detection method comprises: providing a sample template requiring detection; adding dNTP, a 10*buffer, primers, DNA polymerase, a sample requiring detection and ddH2O to a PCR thin-walled tube, and uniformly mixing; amplifying the mixture in the thin-walled tube through a PCR instrument; carrying out electrophoresis on the amplification product in electrophoresis equipment; and analyzing and judging the result. According to the present invention, the PCR kit preparation method is simple and is suitable for industrial production; the PCR detection method has characteristics of high Streptococcus equi detection sensitivity, short detection period, rapidness, and strong operability, wherein detection accuracy can achieve template DNA of 80 bacterial while a detection cost is relatively low; and the detection kit and the detection method have important application values in clinical diagnosis of the disease.

The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.

The invention belongs to the technical field of hyaluronic acid preparation, and particularly relates to a method for high-yield hyaluronic acid by immobilizing microorganisms through a 3D printing technology, GelMA is prepared through a reaction of gelatin and a methacrylic acid esterification reagent, then Gel, GelMA and a photoinitiator are mixed to prepare bio-ink based on Gel-GelMA, and the bio-ink is applied to preparation of high-yield hyaluronic acid. Then, streptococcus equi subspecies for producing hyaluronic acid are uniformly loaded into bio-ink based on Gel-GelMA, then, an immobilized reactor loaded with microorganisms is prepared through a 3D printing technology, and finally, hyaluronic acid is produced in fermentation liquor containing carbon source and nitrogen source substances. The method provided by the invention can solve the defects of low yield, high cost and the like of the current planktonic microorganism direct fermentation method, improves the yield of hyaluronic acid, and can easily separate hyaluronic acid from surrounding media by utilizing a structure containing immobilized bacteria, thereby simplifying the acquisition process of hyaluronic acid.