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Swine Streptococcosis trivalent inactivated vaccine and preparation method thereof

A technology of Streptococcus suis and inactivated vaccine, applied in the direction of antibacterial drugs, bacterial antigen components, etc., can solve the problems of reduced feed rate, economic loss of pig industry, and large stress of pigs, and achieves improvement of piglet survival rate, The effect of reducing pig stress and reducing morbidity

Active Publication Date: 2013-03-06
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, Streptococcus suis type 2 disease has been widespread and prevalent in my country. It has become a major epidemic disease that seriously endangers the development of my country's pig industry, and has also caused serious harm to humans; The disease has a rising trend, its high incidence rate, long incubation period, resulting in reduced feed rate, causing huge economic losses to the pig industry; my country's pig farms still happen from time to time, seriously endangering my country's pig industry
Currently commercially available commercial vaccines are only Streptococcus suis type 2 and Streptococcus suis type 2 + Streptococcus equi subsp. Moreover, commercially available commercial vaccines need to be immunized multiple times, the cost is high, and the stress on pigs is relatively large

Method used

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  • Swine Streptococcosis trivalent inactivated vaccine and preparation method thereof
  • Swine Streptococcosis trivalent inactivated vaccine and preparation method thereof
  • Swine Streptococcosis trivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Isolation and identification of Streptococcus suis 2-LT strain.

[0045] The disease material to be separated—the tissue disease material was obtained by the applicant's inventor in September 2004 from a pig farm with neurological symptoms and acute death in a pig farm in Luotian, Hubei. The specific separation method is as follows: aseptically collect heart blood and inoculate it on a TSA plate (purchased from BD Company, USA), and observe after incubating at 37° C. for 12-24 hours. Pick small colonies with a diameter of 0.1mm-1.0mm, off-white, translucent, smooth, round, and with neat edges, and inoculate them in TSB liquid medium for overnight culture on a shaker at 37°C. Purely cultured bacteria were subjected to Gram staining for microscopic examination. Then observed with an optical microscope, Gram staining was positive, doubled or short streptococci, which were subcultured and purified.

[0046] The classification and identification of Streptococcus...

Embodiment 2

[0067] Example 2: Isolation and identification of Streptococcus suis 7-YZ.

[0068] Isolation of Streptococcus suis type 7 YZ

[0069] The disease material to be separated—the disease material of each tissue was obtained by the applicant's inventor in August 2005 from the disease material submitted for inspection by a pig farm in Yongzhou City, Hunan Province. The specific separation method is as follows: aseptically collect heart blood, lung tissue, spleen tissue, and synovial fluid, inoculate them on a TSA plate, and observe after culturing at 37°C for 12-24 hours. Pick small colonies with a diameter of 0.1mm-1.0mm, off-white, translucent, smooth, round, and with neat edges, and inoculate them in TSB liquid medium for overnight culture on a shaker at 37°C. Purely cultured bacteria were subjected to Gram staining for microscopic examination. Then observed with an optical microscope, Gram staining was positive, double or short streptococcus, which was subcultured and purifie...

Embodiment 3

[0092] Example 3: Isolation and identification of Streptococcus equi subspecies zooepidemicus XS

[0093] The diseased material to be separated—the diseased material of each tissue was obtained by the inventor of the applicant in April 2001 from a diseased pig joint in a pig farm in Xiaoshan, Zhejiang. The specific separation method is as follows: aseptically take samples from heart blood, lung tissue, spleen tissue, joint fluid and other disease materials of suspected diseased pigs, first inoculate them by streaking on TSA solid medium, culture them at 37°C for 24-48 hours, and then pick them. Take a single colony and perform a pure culture. Pick the smear of the above-mentioned pure culture colony, it can be seen that Gram staining is positive, double or short streptococci. It was named Streptococcus equi subsp. zooepidemicus XS.

[0094] Taxonomic identification of Streptococcus equi subspecies zooepidemicus XS

[0095] The latex agglutination test, biochemical identific...

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Abstract

The invention belongs to the technical field of preparation of animal vaccines and particularly relates to a trivalent inactivated vaccine for Streptococcus equi subsp. zooepidemicus, Streptococcus suis serotype 2 and Streptococcus suis serotype 7, as well as a preparation method and application thereof. The inactivated vaccine disclosed by the invention is mainly applicable to prevention and treatment of swine Streptococcosis for sows, boars and piglets. The invention discloses separation, identification and application of three strains special for preparing the swine Streptococcosis trivalent inactivated vaccine, wherein the three strains for the vaccine are respectively a Streptococcus suis serotype 2-LT strain (with the collection number being CCTCC NO:M2011282), a Streptococcus suis serotype 7-YZ strain (with the collection number being CCTCC NO:M2011160) and a Streptococcus equi subsp. Zooepidemicus XS strain (with the collection number being CCTCC NO:M2011405). The inactivated vaccine disclosed by the invention can achieve multiple prevention effects with one injection, so that the stresses of swine are reduced, and the inactivated vaccine is safe and reliable and is free from the potential hazards of toxin spreading.

Description

technical field [0001] The invention belongs to the technical field of animal vaccine preparation, and in particular relates to a trivalent inactivated vaccine of Streptococcus equi subsp. The vaccine of the invention is mainly suitable for the prevention and treatment of sows, boars and piglets against streptococcus suis. technical background [0002] In recent years, the infection rate and incidence of streptococcus suis in intensive pig farms at home and abroad have been increasing day by day, and the economic losses caused by it have become increasingly serious. It has become one of the main diseases that endanger the modern pig industry. It is known that there are 33 serotypes of Streptococcus suis, of which type 2 can cause acute sepsis, meningoencephalitis, arthritis and acute death in pigs, and can also cause infection and death of specific groups of pig practitioners. It is an important Zoonotic infectious diseases (Wisselink et al., 2002). Overseas, since the Net...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61P31/04
Inventor 金梅林康超陈波尹珍艳徐高原陈关平滑亚峰陈章表
Owner HUAZHONG AGRI UNIV
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