7 results about "Streptococcus zooepidemicus" patented technology

The present invention relates to a Streptococcus zooepidemicus SMT013 strain having increased hyaluronic acid production capacity, a variant thereof, and a method for producing hyaluronic acid using the same. According to one aspect, the Streptococcus zooepidemicus SMT013 strain has increased hyaluronic acid production capacity and can be used to produce hyaluronic acid.

The invention discloses a method for producing sodium hyaluronate by regulating and controlling plant peptone based on exogenous amino acid and an exogenous amino acid culture medium. The method for producing the sodium hyaluronate through the exogenous amino acid culture medium comprises the following steps: S1, preparing a seed solution, namely inoculating streptococcus zooepidemicus into the seed culture medium for culturing to obtain the seed solution; s2, fermentation culture: inoculating the seed liquid obtained in the step S1 into the fermentation culture medium for fermentation culture to obtain fermentation liquid; s3, extraction and purification: extracting and purifying the fermentation liquor obtained in the step S2 to obtain a sodium hyaluronate product. According to the method disclosed by the invention, a specific variety of amino acids are exogenously added to promote the growth of thalli, so that high yield and high molecular weight of sodium hyaluronate are realized on the premise of completely using a plant nitrogen source, and meanwhile, the stability, safety and repeatability of a production process are ensured.

This invention proposes a hyaluronic acid and its preparation method, belonging to the field of microbial technology. The hyaluronic acid produced by *Rhizobium pusa* QY124aN in this invention solves the problem of high cultivation costs of existing strains and the lack of a technical method for producing high molecular weight hyaluronic acid using *Rhizobium pusa*. The hyaluronic acid powder prepared by this invention has a hyaluronic acid content of 57.68% and is safer than hyaluronic acid obtained from *Streptococcus vesiculosus* in existing technologies.

The invention discloses a target gene, a primer pair, a kit and a detection method for detecting multi-animal streptococcus and application, and relates to the technical field of bioengineering. The nucleotide sequence of the target gene for detecting the multi-animal streptococcus is as shown in SEQ ID NO: 1; the sequences of the primer pair designed based on the target gene are shown as SEQ ID NO: 2 and SEQ ID NO: 3; the fluorescent quantitative PCR kit for detecting the multi-animal streptococcus comprises a primer pair and a fluorescent quantitative PCR reaction premixed solution, the fluorescent quantitative PCR method for detecting the multi-animal streptococcus comprises the following steps: establishing a standard curve between a multi-animal streptococcus amplification CT value and a template copy number, and quantifying the multi-animal streptococcus in a sample to be detected through the standard curve. Clinical application proves that multi-animal streptococcus in mouse lung tissues and clinical swab samples can be effectively detected. The invention provides reliable technical support for rapid diagnosis and epidemiological investigation of multi-animal streptococcus.

The application discloses a fermentation method for high-yield hyaluronic acid, and belongs to the technical field of microbial fermentation. szhasABC The method adopts recombinant lactococcus carrying a gene cluster from streptococcus zooepidemicus as a production strain, externally adds leech-derived hyaluronidase in a fermentation process, and dynamically controls fermentation temperature (30-37 DEG C). The strategy ingeniously couples the biosynthesis of HA with controllable enzymolysis process, effectively releases the feedback inhibition of high-molecular-weight HA capsule on cell growth, and significantly improves the yield. Meanwhile, through accurate adjustment of process parameters, precise customization of product molecular weight is realized. Experimental results show that, by using the method, the yield of hyaluronic acid can reach 4.6 g / L at most, the molecular weight can be regulated in the range of 0.4 kDa to 850 kDa as required, and the deviation rate is less than 5%. The application does not need to perform complex gene editing on the host, does not depend on the preservation of specific engineering strains, has the advantages of high safety, simple process, low cost, strong controllability and the like, and is suitable for the large-scale production demand of different specifications of hyaluronic acid in the fields of food, cosmetics and medicine.

The present invention relates to a Streptococcus zooepidemicus SMT013 strain having an increased ability to produce hyaluronic acid, a variant thereof, and a method for producing hyaluronic acid using same. The Streptococcus zooepidemicus SMT013 strain according to an aspect has an increased ability to produce hyaluronic acid, and thus can be used to produce hyaluronic acid. Also, provided are a medium composition for a microorganism producing hyaluronic acid, the medium composition comprising arginine and uridine, and a method for producing hyaluronic acid using same. The medium composition for a microorganism producing hyaluronic acid, according to an aspect, can be used to produce hyaluronic acid with increased efficiency.

This invention provides a sodium hyaluronate seed culture and its preparation method. It uses *Streptococcus vesicae* as the production strain and glucose as the carbon source. The culture medium contains 0.5%–1% monosodium glutamate (MSG), and the glucose is separately sterilized at a temperature lower than that of the culture medium. The sodium hyaluronate seed culture is prepared using the following steps: strain resuscitation, strain transfer, and seed tank culture, without any shake-flask culture. By omitting the shake-flask culture step, optimizing the culture medium ratio, and using glucose for separate low-temperature sterilization, the preparation cycle is shortened, the risk of contamination and carbon source loss is reduced, the cell concentration is increased, and the fermentation cost is reduced. This significantly improves the cell concentration of the seed culture and the subsequent fermentation yield, adapting to different scale production needs and effectively reducing the production cost of sodium hyaluronate fermentation.