4 results about "Streptococcus zooepidemicus" patented technology

The present invention relates to a Streptococcus zooepidemicus SMT013 strain having increased hyaluronic acid production capacity, a variant thereof, and a method for producing hyaluronic acid using the same. According to one aspect, the Streptococcus zooepidemicus SMT013 strain has increased hyaluronic acid production capacity and can be used to produce hyaluronic acid.

The application discloses a fermentation method for high-yield hyaluronic acid, and belongs to the technical field of microbial fermentation. szhasABC The method adopts recombinant lactococcus carrying a gene cluster from streptococcus zooepidemicus as a production strain, externally adds leech-derived hyaluronidase in a fermentation process, and dynamically controls fermentation temperature (30-37 DEG C). The strategy ingeniously couples the biosynthesis of HA with controllable enzymolysis process, effectively releases the feedback inhibition of high-molecular-weight HA capsule on cell growth, and significantly improves the yield. Meanwhile, through accurate adjustment of process parameters, precise customization of product molecular weight is realized. Experimental results show that, by using the method, the yield of hyaluronic acid can reach 4.6 g / L at most, the molecular weight can be regulated in the range of 0.4 kDa to 850 kDa as required, and the deviation rate is less than 5%. The application does not need to perform complex gene editing on the host, does not depend on the preservation of specific engineering strains, has the advantages of high safety, simple process, low cost, strong controllability and the like, and is suitable for the large-scale production demand of different specifications of hyaluronic acid in the fields of food, cosmetics and medicine.

The present invention relates to a Streptococcus zooepidemicus SMT013 strain having an increased ability to produce hyaluronic acid, a variant thereof, and a method for producing hyaluronic acid using same. The Streptococcus zooepidemicus SMT013 strain according to an aspect has an increased ability to produce hyaluronic acid, and thus can be used to produce hyaluronic acid. Also, provided are a medium composition for a microorganism producing hyaluronic acid, the medium composition comprising arginine and uridine, and a method for producing hyaluronic acid using same. The medium composition for a microorganism producing hyaluronic acid, according to an aspect, can be used to produce hyaluronic acid with increased efficiency.

This invention provides a sodium hyaluronate seed culture and its preparation method. It uses *Streptococcus vesicae* as the production strain and glucose as the carbon source. The culture medium contains 0.5%–1% monosodium glutamate (MSG), and the glucose is separately sterilized at a temperature lower than that of the culture medium. The sodium hyaluronate seed culture is prepared using the following steps: strain resuscitation, strain transfer, and seed tank culture, without any shake-flask culture. By omitting the shake-flask culture step, optimizing the culture medium ratio, and using glucose for separate low-temperature sterilization, the preparation cycle is shortened, the risk of contamination and carbon source loss is reduced, the cell concentration is increased, and the fermentation cost is reduced. This significantly improves the cell concentration of the seed culture and the subsequent fermentation yield, adapting to different scale production needs and effectively reducing the production cost of sodium hyaluronate fermentation.