Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for producing sodium hyaluronate and preparation method of culture medium

A sodium hyaluronate and culture medium technology, applied in the biological field, can solve the problems of low production efficiency and low yield, and achieve the effects of promoting stable production, high fermentation yield, and reducing the introduction of impurities

Inactive Publication Date: 2019-01-08
SHANGHAI JINGFENG PHARMA
View PDF11 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the output produced by this method is low, and the production efficiency is not high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This embodiment provides a kind of medium for producing sodium hyaluronate

[0047] 100kg medium consists of the following components: white sugar 31kg, yeast extract powder 10kg, MgSO 4 ·7H 2 O 0.9kg, NaH 2 PO 4 2H 2 O 1.25kg, K 2 SO 4 0.8kg, L-arginine 16kg, polyether defoamer 23mL, manganese nitrate 0.3kg, the balance is water.

[0048] Preparation method: Add white sugar, yeast extract powder and L-arginine into water together, stir for 90 minutes at 40°C for the first time until the mixture is uniform, then add polyether defoamer, MgSO 4 ·7H 2 O, NaH 2 PO 4 2H 2 O, K 2 SO 4 and manganese nitrate were slowly added to the mixture, heated to 60°C, stirred for a second time at 700 rpm for 150 min, and the mixture was filtered to remove undissolved impurities to obtain a culture medium.

Embodiment 2

[0050] This embodiment provides a kind of medium for producing sodium hyaluronate

[0051] 100kg medium consists of the following components: white granulated sugar 30kg, yeast extract powder 11kg, MgSO 4 ·7H 2 O 1.0kg, NaH 2 PO 4 2H 2 O 1.3kg, K 2 SO 4 0.8kg, L-arginine 20kg, polyether defoamer 25mL, lanthanum nitrate 0.8kg, the balance is water.

[0052] Preparation method: add white granulated sugar, yeast extract powder and L-arginine to water together, stir for the first time at 30°C for 30 minutes until the mixture is uniform, then add polyether defoamer, MgSO 4 ·7H 2 O, NaH 2 PO 4 2H 2 O, K 2 SO 4 and lanthanum nitrate were slowly added to the mixture, heated to 70°C, stirred for a second time at 1000 rpm for 360 min, and the mixture was filtered to remove undissolved impurities to obtain a culture medium.

Embodiment 3

[0054] This embodiment provides a kind of medium for producing sodium hyaluronate

[0055] 100kg medium consists of the following components: white granulated sugar 32kg, yeast extract powder 10kg, MgSO 4 ·7H 2 O 0.9kg, NaH 2 PO 4 2H 2 O 1.25kg, K 2 SO 4 0.77kg, L-arginine 21kg, polyether defoamer 20mL, ferrous sulfate 0.2kg, the balance is water.

[0056] Preparation method: add white granulated sugar, yeast extract powder and L-arginine into water together, stir for the first time at 50°C for 180 minutes until the mixture is uniform, then add polyether defoamer, MgSO 4 ·7H 2 O, NaH 2 PO 4 2H 2 O, K 2 SO 4 Slowly add ferrous sulfate and ferrous sulfate into the mixture, heat to 55°C, stir for a second time at 500 rpm for 120 min, filter the mixture, remove undissolved impurities, and obtain a culture medium.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
massaaaaaaaaaa
massaaaaaaaaaa
volumeaaaaaaaaaa
Login to View More

Abstract

The invention provides a culture medium for producing sodium hyaluronate and a preparation method of the culture medium. Each 100kg of culture medium comprises the following components: 28 to 35kg ofwhite granulated sugar, 8 to 12 kg of yeast extract powder, 0.8 to 1.1kg of MgSO4.7H2O, 1.2 to 1.4kg of NaH2PO4.2H2O, 0.5 to 1.0kg of K2SO4, 15 to 25kg of L-arginine and 15 to 30mL of a defoamer, andthe components are supplemented to be a total mass of 100kg with water. The culture medium provided by the invention simplifies the complexity of a formula, reduces impurities brought in, ensures thatthe yield of sodium hyaluronate can reach 7.5 to 8.5g / L when streptococcus zooepidemicus H23 is used for fermentation, and compared with the culture medium disclosed in the existing method, the culture medium provided by the invention is higher in fermentation yield in same conditions, facilitates industrial production, and improves the production efficiency.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a culture medium for producing sodium hyaluronate and a preparation method thereof. Background technique [0002] Hyaluronic acid is a kind of mucopolysaccharide necessary for human physiology, which widely exists in various tissues of animals and human body. Based on the high viscosity, lubricity, water retention, good biocompatibility and special physiological properties of hyaluronic acid, it has a wide range of uses in the field of medicine. At present, it is widely used in orthopedics, ophthalmology, surgery, trauma, sustained release Preparations, inducible targeted drug delivery carriers, tumor therapy, dermal fillers, etc. are all being used. [0003] Generally speaking, to prepare sodium hyaluronate raw material, sodium hyaluronate is prepared by the traditional cockscomb extraction method, but there are many defects in the cockscomb extraction method, and the existence of th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12N1/20C12R1/46
CPCC12N1/20C12P19/26
Inventor 陈仁爱金修建沈琳陈依依
Owner SHANGHAI JINGFENG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com