Efficient process for purification of high molecular weight hyaluronic acid

A technology of hyaluronic acid and molecular weight, applied in the effective field for purifying high-molecular-weight hyaluronic acid, can solve problems such as environmental hazards, and achieve the effect of saving cost and reducing load
CN101512006AInactive Publication Date: 2009-08-19RELIANCE LIFE SCI PVT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
RELIANCE LIFE SCI PVT
Publication Date
2009-08-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides an efficient and inproved process for the production and purification of high molecular weight hyaluronic acid (HA). One embodiment relates to providing growth conditions for production of high molecular weight HA in Streptococcus zooepidemicus. For example, growth in medium comprising a lower protein concentration, e.g., 1% casein hydrolyzate, and a higher sugar concentration, e.g., 5% sucrose, gave 5-6 g / L of high molecular weight HA in the range of 3.5-4.0 x 10Da. The present invention also provides an efficient process for the purification of HA comprising treatment with silica gel then active carbon, and subsequent diafiltration with less volume of solvent.
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Description

[0001] Cross references to related applications

[0002] This application claims the benefit of Indian Provisional Application Nos. 1065 / MUM / 2006, filed 6th July, 2006 and 1874 / MUM / 2006, filed 13th November, 2006, the contents of which are hereby incorporated by reference in their entirety. Field of Invention

[0003] The present invention relates to improved techniques for the production and purification of hyaluronic acid and its salts. The invention also relates to the production and process optimization and purification of hyaluronic acid and its salts with biomedical applications. Background of the Invention

[0004] Hyaluronic acid (HA) is a naturally occurring biopolymer with biological functions in bacteria and higher animals including humans. Naturally occurring HA can be found in higher animal tissues, especially as a spacer filler (Balazs, Viscosupplementation: a new concept in the treatment of osteoarthritis. J. Rheumatol. Suppl.; 39: 3-9, (Aug. 1993)) . It is...

Claims

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