22 results about "High molecular mass" patented technology

The object of this invention is a lipophilic activity modifier in the form of dimethyl sulfone (MSM) for use as a transdermal penetration enhancer for high molecular weight active substances with a molecular weight (Mw) > 500 Da. Another object of this invention is a cosmetic composition for transdermal application comprising an active substance and a transdermal penetration enhancer, characterized in that the active substance is encapsulated by the transdermal penetration enhancer, which is the lipophilic activity modifier according to claim 1, and the active substance is a high molecular weight active substance with a molecular weight (Mw) > 500 Da. Another object of the present invention is a method for preparing a composition according to the invention, characterized in that the method is carried out by thermal emulsification or cold emulsification, wherein thermal emulsification comprises the following steps: a) mixing dimethyl sulfone and a diluent in a reaction vessel; b) bringing the mixture to 70-85°C; c) stirring the mixture at 70-85°C; the start of homogenization depends on the stage of phase combination; d) cooling the mixture to 20-40°C, preferably 30°C; e) adding a high molecular weight active substance with Mw>500 Da and homogenizing the mixture, preferably for 1-3 min; while cold emulsification comprises the following steps: a) mixing dimethyl sulfone and a diluent in a reaction vessel and dissolving at a temperature maintained at 20-25°C; b) bringing the mixture to a temperature of 15-35°C, preferably ambient temperature or 25°C; c) stirring the mixture at a temperature of 15-80°C, preferably 25°C; the start of homogenization depends on the stage of phase combination; d) adding a high molecular weight active substance with Mw>500 Da and homogenizing the mixture, preferably for 1-3 min.

This invention relates to the fields of bioengineering and fermentation engineering, and discloses a genetically engineered *Streptomyces albopictus* strain that produces high levels of ε-polylysine and its fermentation production process. This genetically engineered strain uses *Streptomyces albopictus* strains acclimated to ε-polylysine tolerance as a chassis, and its genome integrates... ppc and dapf Through expression box, heterogeneous asd Expression cassettes, containing secretory signal peptides lysp Fusion expression cassettes and D404A / K499A point mutations pls Expression cassette. The fermentation process employs a two-stage pH control strategy, maintaining a neutral environment during the cell growth phase and adjusting to an acidic environment during product synthesis. This invention improves ε-polylysine yield through a synergistic strategy of enhancing precursor supply, constructing efflux detoxification channels, and increasing synthase activity; combined with an acidic fermentation process, it effectively inhibits product degradation, yielding a high-molecular-weight and highly uniform target product suitable for industrial production.

The invention relates to a rapid setting liquid lung sealant that forms a highly adherent and elastic hydrogel. Provided are lung sealants comprising two high molecular weight (20 kDa) multi-arm PEG compositions wherein the first PEG composition includes an alkaline buffer and the second PEG composition includes a mildly acidic buffer for optimized set up time and extended working time. In some embodiments, the PEGs further comprise a radioprotectant such as tocopherol. In some embodiments, a colorant is added to one or both PEGs of the lung sealant to improve visualization of the lung sealant against human lung tissue.

Disclosed herein are peptide hydrogel-based formulations. In some embodiments, the peptide hydrogel-based formulations encapsulate antibodies. In some embodiments, the peptide hydrogel-based formulations prevent formation of high molecular weight protein species.

This invention belongs to the field of biochemical processing technology, and specifically relates to a yeast protein with high emulsifying properties and emulsion stability, and its preparation method. The invention first employs ultrasound-assisted pH induction to pretreat the yeast protein, utilizing a physical field to disrupt the protein's aggregation state and increase the reaction interface. Simultaneously, the pH induction treatment further ensures the protein's complete unfolding, exposing the maximum number of glycosylation reaction sites such as amino and carboxyl groups, and releasing more hydrophobic groups from the yeast protein. Finally, the pretreated yeast protein is glycosylated using high-molecular-weight polysaccharides based on high temperature and / or TG enzymatic hydrolysis. The resulting yeast protein exhibits significantly improved emulsifying properties and emulsion stability.

The application belongs to the technical field of synthetic biology, and discloses a high-purity and high-yield industrial preparation method of a high-molecular-weight polynucleotide (PN). The method uses a plasmid containing a PN base sequence as a template, generates long-chain DNA by using a rolling circle amplification (Rolling Circle Amplification) technology, and then realizes efficient separation of the PN segment by restriction enzyme digestion. The template plasmid is designed, the replication initiation site and the enzyme cutting site are embedded in the corresponding ends of the PN sequence, a single continuous segment seamlessly fused with the PN sequence is formed, 100% theoretical recovery of the PN segment is realized, and the subsequent purification process is significantly simplified. Through the fusion design of the plasmid backbone structure and the PN sequence, the application realizes low-cost and easy-to-scale production of the PN prepared by the rolling circle amplification, and improves the yield and purity of the target product. The process realizes industrial preparation of the high-molecular-weight polynucleotide (PN), and provides a new technical path of safe and reliable, efficient and convenient, and scalable amplification for industrial production of the high-molecular-weight PN.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

For example, to provide a method for identifying or selecting patients who are most likely to benefit from treatment with telomerase inhibitors such as imetelstat. [Solution] This disclosure provides a method for identifying or selecting patients who are most likely to benefit from treatment with telomerase inhibitors, such as imetelstat, by testing for high molecular weight risk (HMR) based on the absence of mutations in each of JAK2, CALR, and MPL, and / or the presence of a mutation in at least one of the following genes: ASXL1, EZH2, SRSF2, and IDH1 / 2. These patients may have myelofibrosis. This disclosure also provides a method for treating myelofibrosis, the method of which includes identifying such patients.

Processes and apparatuses for reducing molecular weight fluctuation in a tail gas stream from a pressure swing adsorption process. Multiple PSA separation zone are synchronized such that when one of the PSA units is generating a low molecular weight tail gas, there is another PSA unit generating a high molecular weight tail gas.

The present invention relates, in general, to a method for characterizing crosslinking sites of a target protein. In particular, the present invention relates to identifying and quantifying crosslinking sites of a target protein and determining the contribution of crosslinking to the formation of high molecular weight species using size exclusion chromatography, peptide mapping analysis, and subunit analysis.

The present application relates to a kind of specific synthesis plant inulin of inulin sucrose enzyme mutant and its application, the mutant is the 300th aspartic acid of wild type inulin sucrose enzyme from Lactobacillus reuteri (Lactobacillus reuteri) Limosilactobacillus reuteri ) Mutated into alanine.It precisely cuts off the ability of wild type enzyme to synthesize million Dalton high molecular weight microbial inulin at molecular level, while perfectly retaining the synthesis ability of medium-low degree of polymerization inulin.This mutant fundamentally solves the problem of wild type enzyme product molecular weight distribution extremely wide, component complex, realizes the efficient biosynthesis of specific inulin.At the same time, it can greatly reduce the difficulty and energy consumption of downstream separation and purification process, significantly reduce production cost.In the high-quality inulin large-scale green manufacturing of food and health product industry, it has great application value.

Disclosed herein is a method for diagnosing a mycobacterial infection in a subject. The method comprises performing acetonitrile fractionation of a subject sample to deplete depleted in high molecular weight proteins. The sample is enzymatically digested and analyzed with liquid chromatography tandem mass spectrometry (LC-MS / MS) and bottom-up proteomic analysis to identify a plurality of sample peptides. The sample is then analyzed with a classifier algorithm and a PW species identification score (PWsp) is generated by dividing the number of single and multi-peptide combinations that identify a specific mycobacterium species by the total number of peptide combinations specific for any mycobacteria. A higher PWsp for a given species of mycobacterium indicates a higher likelihood the subject has an infection of that species. This method provides an efficient and accurate way of diagnosing mycobacterial infections in subjects.

This invention discloses a single-base precision DNA double-strand break sequencing library, its construction method, and its applications, belonging to the field of genome detection technology. Specifically, it relates to a method for constructing a single-base precision DNA double-strand break sequencing library, comprising: extracting high-molecular-weight genomic DNA from a sample; then ligating P5 adapters to the ends of DSBs present in the genome; further fragmenting the DSBs using ultrasound; and then ligating semi-functional P7 adapters; enriching the target sequence by PCR to obtain the DSB sequencing library. This invention provides a single-base precision DNA double-strand break sequencing library, its construction method, and its applications. By using the constructed library for DSB sequencing and combining it with bioinformatics analysis strategies to filter false positive background noise, it overcomes the DSB detection bias problem caused by protein masking in existing in situ labeling methods. Simultaneously, it significantly reduces reagent costs, simplifies experimental procedures, and shortens experimental cycles, thereby improving detection efficiency and the potential for large-scale application.

This invention relates to the field of polymer materials technology, and discloses an impact-resistant ultra-high molecular weight polyethylene and its preparation method. The polyethylene material of this invention comprises the following raw materials in parts by weight: 60-70 parts ultra-high molecular weight polyethylene, 5-8 parts maleic anhydride-grafted polyethylene, 5-10 parts reinforcing filler, 0.1-0.5 parts antioxidant, and 1-3 parts lubricant. This material not only retains the high impact resistance of ultra-high molecular weight polyethylene, but also adds reinforcing filler, giving the material excellent wear resistance and heat resistance.

The application discloses a fermentation method for high-yield hyaluronic acid, and belongs to the technical field of microbial fermentation. szhasABC The method adopts recombinant lactococcus carrying a gene cluster from streptococcus zooepidemicus as a production strain, externally adds leech-derived hyaluronidase in a fermentation process, and dynamically controls fermentation temperature (30-37 DEG C). The strategy ingeniously couples the biosynthesis of HA with controllable enzymolysis process, effectively releases the feedback inhibition of high-molecular-weight HA capsule on cell growth, and significantly improves the yield. Meanwhile, through accurate adjustment of process parameters, precise customization of product molecular weight is realized. Experimental results show that, by using the method, the yield of hyaluronic acid can reach 4.6 g / L at most, the molecular weight can be regulated in the range of 0.4 kDa to 850 kDa as required, and the deviation rate is less than 5%. The application does not need to perform complex gene editing on the host, does not depend on the preservation of specific engineering strains, has the advantages of high safety, simple process, low cost, strong controllability and the like, and is suitable for the large-scale production demand of different specifications of hyaluronic acid in the fields of food, cosmetics and medicine.

The application discloses a kind of protein enzymolysis big health food intelligent quick freezing system, belong to food freezing technical field, specifically include: supercooling acquisition module is extracted by pre-freezing test quantitative protein enzymolysis liquid supercooling tendency characterization parameter, parameter search module obtains cold wall surface vibration frequency value and amplitude value from the corresponding table of prestorage according to the parameter, perfusion precooling module is perfused to enzyme solution between movable cold wall surface and starts liquid nitrogen supply and temperature acquisition, heat release identification module identifies crystallization heat release time by comparing the trend reversal of temperature acquisition value statistical result, force field exertion module drives two movable cold wall surface relative reciprocating vibration and applies extrusion-stretching deformation from crystallization heat release time, termination storage module stops vibration and liquid nitrogen supply when temperature reaches supercooling termination storage temperature and stores whole process data according to time stamp alignment.The application can inhibit the damage of ice crystal growth to high molecular weight polypeptide in protein enzymolysis liquid quick freezing process.

This invention discloses a method for creating high-molecular-weight glutenin subunit mutants using single-base editing and its applications. The method includes the following steps: constructing a single-base editing vector by designing sgRNA targeting the tyrosine codon of the HMW-GSs gene; achieving wheat genetic transformation using Agrobacterium-mediated transformation; realizing targeted single-base substitution at target sites (such as position 70 of the 1Bx20 gene and position 73 of the 1Dx5 gene, TAC→TGC), precisely mutating tyrosine to cysteine; and determining bread-processing-related quality parameters of the mutants. This invention uses an adenine base editor to perform targeted editing of the tyrosine codon in wheat HMW-GSs, precisely mutating it to cysteine. This invention is the first to achieve precise editing of HMW-GSs tyrosine to cysteine ​​in wheat, providing a directly applicable theoretical basis and technical path for creating high-quality new wheat germplasm and optimizing bread production processes.

The present application relates to the technical field of extraction reagent, in particular to an economic and general high molecular weight DNA extraction reagent and extraction method, which comprises a lysis solution, a precipitant, a binding solution, a washing solution 1, a washing solution 2, an eluent and a protease K. The present application adopts a precipitation method to extract DNA, avoids using high-priced nucleic acid capture carriers (such as magnetic beads and silica gel membranes) and special extraction instruments, and the reagent components are all conventional inexpensive reagents, so that the cost is reduced by more than 30% compared with existing commercial reagents, and the present application is easy to popularize and widely apply in laboratories.

The present invention relates to mono-, di-, or polyquaternary ammonium compounds of the formula: R 1 (-F) x (I) where x is between 1 and 50, and F may be the same or different and is represented by general formula (II), [Formula 1] JPEG2023506846000373.jpg50164 wherein the optionally substituted and optionally functionalized hydrocarbon radical R 1 , R 2 , R 3 , R 4 or R 5 at least one of which comprises at least one estolide moiety containing two or more ester or amide moieties. The present invention also relates to methods for the synthesis of such compounds, their use in cosmetic skin and hair care formulations, cosmetic compositions for the treatment of fibers, and compositions comprising one or more of the compounds for the treatment of hair.

This invention discloses a method for producing ultra-strong and ultra-thin ultra-high molecular weight polypropylene (BOPP) film, comprising the following steps: (1) isotactic polypropylene, ultra-high molecular weight polypropylene, compatibilizer and antioxidant are blended and melt-extruded, and cooled and shaped to form a cast sheet; (2) the cast sheet is subjected to intermittent biaxial stretching, which means that the stretching is paused at least once during the stretching process, and the film is moved to an environment with a lower temperature than the stretching temperature and kept for a period of time to release the stress accumulated in the molecular chains during stretching, and then the stretching is continued to obtain the BOPP base film; (3) the surface of the BOPP base film is subjected to corona treatment; (4) a chlorinated polypropylene modified acrylic resin primer is coated on the surface of the treated BOPP base film, and the finished film is obtained after drying. This invention achieves effective reduction of film thickness and simultaneous improvement of tensile strength, and endows the film with excellent initial adhesion and water resistance stability.

This utility model discloses a reactor for processing ultra-high molecular weight polyethylene (UHMWPE) that is easy to maintain. It includes a reactor body with a feed inlet on the top right side. The reactor body is connected to processing components, which include: a feed valve installed on the top right side of the reactor body; and a stirring component installed on the reactor body for stirring the raw materials. This utility model relates to the field of UHMWPE processing technology. This easy-to-maintain UHMWPE reactor allows the motor, bevel gear assembly, connecting shaft, connecting plate, and stirring bar to work together to stir the raw materials and reduce residual material retention in the reactor body through scraping, thereby improving raw material utilization and reducing waste. After the UHMWPE is prepared, the water pump, conduit, three-way valve, branch pipe, spray ring, spray bar, connecting shaft, and connecting plate work together to rinse and clean the inner wall of the reactor body and the surface of the stirring bar.