135 results about "High molecular mass" patented technology

The invention relates to the technical field of cutter damage diagnosis, and discloses a fault diagnosis method and system for an ultra-high molecular weight polyethylene crushing device, and the method comprises the steps: obtaining a cutter impact contact image, recognizing an indentation region, extracting an indentation depth residual value and a rebound recovery height, and calculating an indentation rebound factor; collecting a material particle size distribution map, extracting a particle size main peak drift value, a perturbation particle proportion and a distribution full width at half maximum value, and constructing a particle size disturbance factor; performing difference analysis on the indentation springback factors and the particle size disturbance factors of the current batch and the adjacent batch, and weighting to construct an interference dynamic increment factor; and calculating an impact response deviation value based on the factor change trend, and judging whether the tool has a damage trend or a critical fault state or not. According to the invention, the tool wear trend and the critical fault state are identified and diagnosed.

The object of this invention is a lipophilic activity modifier in the form of dimethyl sulfone (MSM) for use as a transdermal penetration enhancer for high molecular weight active substances with a molecular weight (Mw) > 500 Da. Another object of this invention is a cosmetic composition for transdermal application comprising an active substance and a transdermal penetration enhancer, characterized in that the active substance is encapsulated by the transdermal penetration enhancer, which is the lipophilic activity modifier according to claim 1, and the active substance is a high molecular weight active substance with a molecular weight (Mw) > 500 Da. Another object of the present invention is a method for preparing a composition according to the invention, characterized in that the method is carried out by thermal emulsification or cold emulsification, wherein thermal emulsification comprises the following steps: a) mixing dimethyl sulfone and a diluent in a reaction vessel; b) bringing the mixture to 70-85°C; c) stirring the mixture at 70-85°C; the start of homogenization depends on the stage of phase combination; d) cooling the mixture to 20-40°C, preferably 30°C; e) adding a high molecular weight active substance with Mw>500 Da and homogenizing the mixture, preferably for 1-3 min; while cold emulsification comprises the following steps: a) mixing dimethyl sulfone and a diluent in a reaction vessel and dissolving at a temperature maintained at 20-25°C; b) bringing the mixture to a temperature of 15-35°C, preferably ambient temperature or 25°C; c) stirring the mixture at a temperature of 15-80°C, preferably 25°C; the start of homogenization depends on the stage of phase combination; d) adding a high molecular weight active substance with Mw>500 Da and homogenizing the mixture, preferably for 1-3 min.

The present invention relates to a high-molecular-weight hyaluronic acid structure obtained by applying shear stress to a solution containing high-molecular-weight hyaluronic acid, a method for preparing the same, and an ophthalmic composition including the same.

This invention relates to the fields of bioengineering and fermentation engineering, and discloses a genetically engineered *Streptomyces albopictus* strain that produces high levels of ε-polylysine and its fermentation production process. This genetically engineered strain uses *Streptomyces albopictus* strains acclimated to ε-polylysine tolerance as a chassis, and its genome integrates... ppc and dapf Through expression box, heterogeneous asd Expression cassettes, containing secretory signal peptides lysp Fusion expression cassettes and D404A / K499A point mutations pls Expression cassette. The fermentation process employs a two-stage pH control strategy, maintaining a neutral environment during the cell growth phase and adjusting to an acidic environment during product synthesis. This invention improves ε-polylysine yield through a synergistic strategy of enhancing precursor supply, constructing efflux detoxification channels, and increasing synthase activity; combined with an acidic fermentation process, it effectively inhibits product degradation, yielding a high-molecular-weight and highly uniform target product suitable for industrial production.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

A chemical mechanical polishing pad is provided containing a polishing layer having a polishing surface, wherein the polishing layer comprises a reaction product of ingredients, including: an isocyanate terminated urethane prepolymer; and, a curative system, containing a high molecular weight polyol curative; and, a difunctional curative.

The application discloses a method for controllably degrading glycosidic bonds of hyaluronic acid by programmed temperature rising. The method is used for high-molecular-weight hyaluronic acid aqueous solution, and a combination of an oxygen oxidant and an acidic additive is used to controllably cut and degrade glycosidic bonds in the macromolecule through a heating process of programmed temperature rising control system, so as to obtain oligomeric hyaluronic acid. The oligomeric hyaluronic acid prepared by the method has very good stability and no color change after long-time storage. The oligomeric hyaluronic acid prepared by the method can be applied to the fields of cosmetics, food, medicine and the like, and has very strong practical value.

The invention relates to a rapid setting liquid lung sealant that forms a highly adherent and elastic hydrogel. Provided are lung sealants comprising two high molecular weight (20 kDa) multi-arm PEG compositions wherein the first PEG composition includes an alkaline buffer and the second PEG composition includes a mildly acidic buffer for optimized set up time and extended working time. In some embodiments, the PEGs further comprise a radioprotectant such as tocopherol. In some embodiments, a colorant is added to one or both PEGs of the lung sealant to improve visualization of the lung sealant against human lung tissue.

The invention provides a gene editing cell and a method for producing extremely high molecular weight hyaluronic acid by using the cell. The preparation method comprises the following steps: by taking PUC57 as a skeleton carrier, constructing a recombinant plasmid containing an HAS2 gene, and transfecting a mole NMRHas2 gene, a chicken CHICKHAS2 gene, an astronomy mouse SNMHAS2 gene, a dakalimeris mouse DMRHAS2 gene or a mole mouse BMRHAS2 gene into chicken fibroblasts through lipidosome to construct a stably transfected cell line, so that hyaluronic acid with extremely high molecular weight can be stably obtained. The method provided by the invention overcomes the defects of non-uniform molecular weight distribution of HA product sources, low molecular weight and single method in the prior art, and has important significance for developing medicines for treating osteoarthritis and novel medical equipment products.

Disclosed herein are peptide hydrogel-based formulations. In some embodiments, the peptide hydrogel-based formulations encapsulate antibodies. In some embodiments, the peptide hydrogel-based formulations prevent formation of high molecular weight protein species.

The invention relates to the technical field of medical preparations, in particular to moisturizing and repairing type artificial tear as well as a preparation method and application thereof. Comprising the following steps: preparing effective components including high-molecular-weight polydeoxyribonucleotide and hyaluronic acid or a salt thereof into a homogeneous-phase stable composite solution, sterilizing, filtering, and aseptically packaging, the artificial tear formed by selecting high-molecular-weight polydeoxyribonucleotide and high-molecular-weight sodium hyaluronate as effective components has a relatively good repairing effect on tissues, can form long-term moisturizing and lubrication on ocular surfaces, is suitable for treating xerophthalmia, repairing cornea, relieving inflammatory response and the like, and has a good application prospect. Important market values and clinical application prospects are realized.

An ultra-high molecular weight polyethylene (UHMWPE) polymer is provided having an average molecular weight of at least 2,000,000 g / mol and an enthalpy of at least 190 J / g. The UHMWPE polymer can include at least one comonomer. The UHMWPE polymer is used to form a membrane, which when stretched has a node and fibril structure. The UHMWPE membrane has an endotherm of about 150° C. associated with the fibrils in the membrane. The membrane has a porosity of at least 60% and a bubble point of 138 kPa or less. Additionally, the UHMWPE membrane has a thickness of less than 1 mm. The UHMWPE membrane can be formed by lubricating the UHMWPE polymer, subjecting the lubricated polymer to pressure at a temperature below the melting point of the UHMWPE polymer to form a tape, and stretching the tape at both temperatures below and above the melting temperature of the UHMWPE polymer.

This application provides high molecular weight block copolymers comprising at least one block A comprising repeating units derived from monomers comprising lactam and acryloyl moieties and at least one block B comprising repeating units derived from hydrophilic monomers. The application further provides compositions comprising the block copolymers and applications thereof in various industrial areas including personal care. The application furthermore provides compositions comprising colloidal particles of the block copolymers. The variables x, y, R8 and R9 are described herein (I). Wherein each x and y is independently an integer having a value from about 10 to about 10000, and each R8, and R9 is independently selected from the group consisting of hydrogen, methyl, and combinations thereof.

The invention belongs to the field of chemical synthesis, and particularly relates to a chemical synthesis method of elastin-like polypeptide. The invention provides a chemical synthesis method of elastin-like, which comprises the following steps of: constructing a pentapeptide repetitive sequence (valine-proline-glycine-X-glycine, X is amino acid except proline, and the amino acid except glycine is respectively and independently selected from levoamino acid or dextroamino acid) of elastin-like polypeptide through liquid phase reaction; the elastin-like polypeptide can be prepared with the target molecular weight of 1000-500000 by adding amino groups and carboxyl groups into an organic solvent, forming orthogonal protection on the amino groups and the carboxyl groups, then selectively removing amino or carboxyl end protecting groups respectively, accurately coupling the amino groups and the carboxyl groups in a solution and repeating the deprotection and coupling processes for multiple times. The preparation method can realize accurate regulation and control of the molecular weight, sequence, chirality and topological structure of the elastin-like protein, improves the synthesis efficiency of the high-molecular-weight elastin-like polypeptide, has the advantages of large synthesis scale, low cost and the like, and has a wide application prospect.

The present application relates to the field of sodium hyaluronate preparation, in particular to a preparation method of low molecular weight sodium hyaluronate. Specifically comprising the following steps: stirring and dissolving macromolecular weight sodium hyaluronate powder into water at 45-60 DEG C to obtain a macromolecular weight sodium hyaluronate solution; cooling to 35-45 DEG C, adding a buffer solution and mixing uniformly, then adding papain and hyaluronidase with a mass ratio of (2-3):(3-6) for enzymatic reaction to obtain an enzymatic solution; adding sodium hydroxide solution to the enzymatic solution, adjusting pH to 7.5-9.0, heating to 80-90 DEG C for 20-40 minutes, then filtering to obtain a low molecular weight sodium hyaluronate solution; spray drying, and the process is simple, the conditions are mild, the product structure is not damaged, and the prepared low molecular weight sodium hyaluronate has high purity, uniform molecular weight distribution and good permeability.

The utility model relates to an automatic flanging device for an ultra-high molecular weight polyethylene pipe, and belongs to the field of polymer pipe flanging. Comprising a flanging assembly, a pipe containing assembly and a movable sliding frame, the pipe containing assembly comprises a positioning barrel and a connecting plate, the bottom of the positioning barrel is fixed to the movable sliding frame through the connecting plate, a supporting ring is arranged at the top of the positioning barrel, and the flanging assembly comprises a heating sleeve, a fixing frame, a lifting air cylinder, a flanging mold and a flanging air cylinder. The heating sleeve is arranged above the positioning cylinder; the heating sleeve is fixedly connected with a fixing frame, and the fixing frame is lifted up and down through a lifting air cylinder. A flanging air cylinder is fixed to the fixing frame, a flanging die is arranged in the heating sleeve, a hole is formed in the center of the top of the heating sleeve, and a push rod of the flanging air cylinder penetrates through the hole to be connected with the flanging die. According to the utility model, the flanging efficiency and the automation degree are improved. And different types of ultra-high molecular weight polyethylene pipes can be subjected to flanging operation by replacing the positioning cylinder, so that the applicability is high.

The human HAS2 expression vector based on the plant suspension cell system and the efficient production method of the human HAS2 expression vector have the advantages of being free of animal source pollution, free of cell disruption, capable of achieving secretory expression and the like, and prepared hyaluronic acid is high in molecular weight (gt, 0.8 MDa) and high in purity and has good medical and food-grade application prospects.

The present invention provides methods of using a composition comprising one or more bacterial species of the Bacillus genus, rice bran, L-cysteine and a high molecular weight low osmolality carbohydrate for the treatment and / or prevention of type 2 diabetes, pre-diabetes, and / or a symptom thereof in a subject. Further uses of the composition are also provided herein.

The invention relates to the technical field of active peptide chelate preparation, and discloses a high-chelation-rate colla corii asini peptide-metal ion chelate preparation method, which comprises: preparing a colla corii asini protein solution; adding a metal ion solution into the colla corii asini protein solution to carry out pre-reaction so as to construct a metal ion template; then adding a core function protease with a self-speed-limiting characteristic on a product, and carrying out synchronous enzymolysis-chelation reaction under the guidance of a template; and finally terminating the reaction to obtain a product. According to the method, oriented cutting of protease hydrolysis sites is realized by pre-constructing a metal ion template, and excessive hydrolysis of the product is avoided by utilizing the self-speed-limiting characteristic of the core function protease. Enzymolysis and chelation reaction are carried out synchronously, so that the process flow is obviously simplified, and the loss of intermediate products is avoided. The colla corii asini peptide-metal ion chelate prepared by the method has the technical effects of high chelation rate, concentrated molecular weight distribution and high utilization rate of raw material protein.

The invention discloses a preparation method of high-molecular-weight polyvinyl ether, and relates to the technical field of organic synthesis. Comprising the following steps: 1, adding vinyl ethyl ether into o-xylene, and adding alcohol and a catalyst to initiate polymerization; and 2, carrying out low-temperature polymerization, after the reaction is finished, adding deionized water, carrying out liquid separation and distillation, and adding o-xylene to adjust the solid content so as to form a solution, thereby obtaining the high-molecular-weight polyvinyl ether. Vinyl ethyl ether is dissolved in o-xylene, a catalyst is added at the temperature of 30-40 DEG C to initiate a polymerization reaction, polymerization continues at the temperature of-10-10 DEG C, and light yellow transparent viscous liquid, namely the high-molecular-weight polyvinyl ethyl ether, is obtained. The method is carried out in the solvent, the solvent does not need to be removed and recycled, the reaction route operation is simple, and the polymer molecular weight is controllable. The method has the advantages of no by-product and high yield.

The invention belongs to the field of microorganisms and food processing, and particularly discloses a fungal protein hydrolysate, a preparation method thereof and application of the fungal protein hydrolysate in meat flavor. According to the method disclosed by the invention, firstly, fungi are treated by a combined means of alkali liquor suspension, high-pressure homogenization and heating, so that the extractability of fungal protein is improved; then, protease is adopted for carrying out directional cutting on hydrophobic amino acid residue sites, and the protein hydrolysate rich in branched chain amino acid is obtained. The hydrolysate has the following advantages: (1) the content of branched chain amino acids (leucine, isoleucine and valine) is high; the total content of branched chain amino acids accounts for more than 20% of the total content of free amino acids; (2) the proportion of low-molecular-weight peptide is high; the mass percentage content of the protein peptide fragment with the molecular weight of less than 3 kD is not less than 80%; and (3) good Maillard reaction activity is achieved, and rich meat flavor substances can be generated under the heating condition.

The invention discloses a poly HRP-nano antibody conjugate as well as a preparation method and application thereof. The invention aims to provide a method for generating a plurality of active aldehyde groups on the surface of horse radish peroxidase through systematically optimizing key parameters such as reaction temperature, oxidation time and reactant molar ratio by utilizing a periodate oxidation method; then, the activated HRP reacts with a primary amine group on the nano antibody to form a high-molecular-weight functional polymer conjugate; according to the scheme, the advantage of small molecular weight of the nano antibody is utilized, and the limitation that the traditional IgG antibody is difficult to form a high-valence functional polymer due to a steric hindrance effect in coupling is overcome; the prepared conjugate can remarkably improve the detection sensitivity of immunoassay, the reaction yield is high, a crude product of the conjugate can be directly applied to detection without purification, the preparation process is greatly simplified, the application cost is reduced, and a simple, convenient, universal and efficient solution is provided for developing a novel high-sensitivity immunodetection reagent; belongs to the field of biotechnology and in-vitro diagnosis.

This invention discloses a polyethersulfone, its preparation method, and its applications. The polyethersulfone satisfies the following formula: x = A1 / (A1+A2) < 6%; where A1 is the integral area occupied by the weight-average molecular weight of not less than 200,000 g / mol in the GPC spectrum, and A1 is denoted as the high molecular weight peak area; A2 is the integral area occupied by the weight-average molecular weight of less than 200,000 g / mol in the GPC spectrum, and A2 is denoted as the main peak area. The polyethersulfone prepared by this invention exhibits a bimodal molecular weight distribution, with the main peak area accounting for more than 90%. The main peak molecular weight can be controlled, and the weight-average molecular weight is between 60,000 and 180,000 g / mol, which can meet the performance requirements of applications such as hemodialysis membranes.

The invention discloses a method for extracting and identifying a high molecular weight glutenin subunit (HMW-GS) in wheat grains in an early stage, which comprises the following steps: taking the wheat grains 15 days after flowering and before a dough stage, and extracting by using 80-160 [mu] l of an extraction buffer solution (40% of isopropanol, 200mM of Tris-HCl, pH 8.0, 0.75% of DTT and 0.5% of 4-VP). The extraction and identification method is suitable for early identification of high molecular weight glutenin subunit types in wheat grains, and is also suitable for identification of glutenin subunit types in harvested seeds. The seeds 15 days after flowering and before the grain dough stage are high in moisture content, low in grain hardness and easy to grind, early sampling accelerates the extraction and detection efficiency of the wheat grain high-molecular-weight glutenin subunit, and accelerates the wheat quality breeding process.

The invention discloses a polypeptide targeting CLDN4 protein as well as a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The amino acid sequence of the polypeptide of the targeted CLDN4 protein is as follows: YSGNYPYSIL, QPALNLYRWY or RPALNLYRWY. The polypeptide can specifically target CLDN4 positive cells, is high in selectivity, and can be applied to preparation of drugs or drug carriers for treating ovarian cancer; the polypeptide can be prepared by a chemical synthesis method, and is high in purity, small in molecular weight, strong in specificity, free of immunogenicity, safe and reliable.

The invention discloses an efficient adenovirus packaging method, and belongs to the technical field of genetic engineering. The method comprises the following steps: culturing and packaging cells; aAV carrier plasmids, AAV packaging plasmids and adenovirus helper plasmids are mixed according to an optimized molar ratio (preferably 1: 1: 1.2) with high molecular weight polyethyleneimine (PEI) according to an optimized mass ratio (preferably 3: 1) to form a transfection compound for transfecting cells; after transfection, adding sodium butyrate and reducing the culture temperature to synergistically promote virus packaging and accumulation. According to the method, transfection key parameters and culture conditions are systematically optimized, the AAV packaging efficiency and the total virus yield are remarkably improved, the production cost is reduced, and the method is stable in process, easy to amplify and suitable for large-scale production of AAV gene therapy drugs.

This invention belongs to the field of biochemical processing technology, and specifically relates to a yeast protein with high emulsifying properties and emulsion stability, and its preparation method. The invention first employs ultrasound-assisted pH induction to pretreat the yeast protein, utilizing a physical field to disrupt the protein's aggregation state and increase the reaction interface. Simultaneously, the pH induction treatment further ensures the protein's complete unfolding, exposing the maximum number of glycosylation reaction sites such as amino and carboxyl groups, and releasing more hydrophobic groups from the yeast protein. Finally, the pretreated yeast protein is glycosylated using high-molecular-weight polysaccharides based on high temperature and / or TG enzymatic hydrolysis. The resulting yeast protein exhibits significantly improved emulsifying properties and emulsion stability.

The invention belongs to the field of polymer chemical recovery, and discloses a method for preparing high-molecular-weight and mechanical-property PCL (polycaprolactone) through efficient polycondensation of low / medium-molecular-weight PCL. The method comprises the following steps: heating dried low / medium molecular weight PCL and a catalyst to 140-270 DEG C under the pressure condition of 10-500KPa, carrying out polycondensation reaction for 3-24 hours, and separating water generated by the polycondensation reaction from a reaction system in the reaction process to obtain high molecular weight PCL. The method is simple, efficient chemical recycling of low / medium molecular weight PCL is achieved, and high molecular weight and mechanical property PCL is formed through polycondensation. The molecular weight of the product is as high as 5.08 * 10 < 4 > g / mol, and the tensile strength and the elongation at break of the product respectively reach 31.1 MPa and 1420%, which are superior to those of commercial PCL.

The invention relates to methods for extracting, purifying and concentrating mesenchymal stromal cell exosomes enriched with micro-RNA, proteins and lipids, and can be used in cosmetology and pharmacology for rehabilitation and regeneration. The technical result of the invention is a more efficient process for producing exosomes. This technical result is achieved in that the present method for extracting, purifying and concentrating exosomes from mesenchymal stromal cells includes cultivating cells, collecting the conditioned culture medium, depleting high-molecular-weight proteins, extracting, purifying and concentrating exosomes, and freezing and lyophilizing the exosome concentrate; in order to enrich the culture medium with exosomes, multipotent mesenchymal stem cells (MSCs) are grown in a medium having a lowered glucose concentration and containing platelet lysate and 5 mM of L-alanyl-L-glutamine, wherein, in the case of static cell cultivation, the growth medium is placed in a culture vessel and once a level of not less than 80% monolayer confluence is reached, the culture medium is harvested and replaced once every 24 hours, and in the case of flow-through cell cultivation, cells are placed on cell carriers in a bioreactor and 72-96 hours after the start of cultivation, the culture medium is harvested and replaced once every 24 hours, the exosome-enriched culture medium is subjected to concentration, which includes successive stages of centrifugation, tangential flow filtration and isopycnic centrifugation: in the first stage, the culture medium is centrifuged for 10 mins at 2000 g to remove large cell particles and other inclusions; in the tangential flow filtration stage, the supernatant is concentrated using hollow fibre filters having a molecular weight cutoff of 500 kDa, after which the concentrate is subjected to fivefold diafiltration with an equal volume of DPBS phosphate buffer solution using the same hollow fibre cartridge; and in the isopycnic centrifugation stage, the concentrated supernatant is mixed with a saccharose solution and deuterium oxide and subjected to isopycnic centrifugation at 100000 g and 4˚С for 75 mins, after which the concentrate is subjected to further fivefold diafiltration with a buffer solution, then the concentrate is filtered through filters with a pore diameter of 0.22 µm and 0.1 µm, the exosome concentrate is subjected to initial freezing to -75˚C in a freezing chamber, and drying is carried out in a lyophilization chamber, wherein in a first drying stage, the concentrate is cooled to -40˚С and subsequently held for 100 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -30˚С and subsequently held for 610 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -10˚С and subsequently held for 315 mins at an absolute pressure in the chamber of not more than 10 Pa, after which it is cooled to 0˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa, then heated to +10˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa; in a second drying stage, drying is carried out at +22˚С for not less than 130 minutes at an absolute pressure in the chamber of not more than 10 Pa.

The invention discloses a preparation method of a chitin-glucan hydrogel with a lipid lowering effect and derived from flammulina velutipes. According to the method, firstly, green and environment-friendly enzyme hydrolysis is combined with weak base for treatment, so that the extraction efficiency of the chitin-glucan compound is improved, and the chitin-glucan compound with high acetylation degree, high molecular weight, high crystallinity and good thermal stability is obtained. Secondly, through physical gel preparation of the hydrogel, compared with the hydrogel prepared by the method, the hydrogel prepared by the method has relatively high mechanical property, rheological property and stability, and shows excellent drug loading and releasing capability. The lipid-lowering effect is remarkably enhanced by loading and encapsulating naringenin through the hydrogel and preparing the naringenin into the gel particles, and the huge prospect of the hydrogel in the lipid-lowering aspect is embodied.