263 results about "High molecular mass" patented technology

The present disclosure provides a method of preparing a lyophilized bispecific molecule composition comprising (a) inducing ice nucleation in a liquid protein composition having a bispecific molecule concentration of at least about 10 mg / ml in a vial exposed to a first temperature of about-18 DEG C to about-10 DEG C for about 60 minutes to about 270 minutes; (b) exposing the vial of (a) to a second temperature of about-25 DEG C to-50 DEG C for about 1 hour to about 5 hours; and (c) drying the composition of (b) at a third temperature of about-5 DEG C to about-25 DEG C for about 25 hours to about 70 hours, and (d) further drying the composition of (c) at a fourth temperature of about 25 DEG C to about 50 DEG C for about 4 hours to about 12 hours to obtain a vial comprising a lyophilized bispecific molecular composition, the composition has a percentage content of high molecular weight material (HMWS) of less than or equal to about 1.5% (mA / ). The disclosure further provides lyophilized bispecific molecule compositions prepared via the methods described herein.

The invention discloses EVA (Ethylene Vinyl Acetate) with high molecular weight and narrow molecular weight distribution and a preparation method thereof, and relates to the technical field of EVA. According to the technical scheme, the preparation method comprises the following steps: in a protective gas atmosphere, adding vinyl acetate and a reaction solvent into a reaction kettle, introducing ethylene gas, replacing the reaction kettle with the ethylene gas environment, then adding an initiator and a molecular weight regulator, and simultaneously heating to 60-80 DEG C for reaction; after the reaction is completed, the reaction kettle is decompressed and cooled, a terminator is added for quenching reaction, and EVA with high molecular weight and narrow molecular weight distribution is obtained. According to the invention, the molecular weight of EVA is improved, and the molecular weight distribution of EVA is reduced, so that the problems of low molecular weight and wide molecular weight distribution of EVOH are solved, and the processability and mechanical property of EVOH are greatly improved.

The invention relates to the field of preparation of sodium hyaluronate, in particular to a preparation method of low-molecular-weight sodium hyaluronate. The method specifically comprises the following steps: stirring and dissolving high-molecular-weight sodium hyaluronate powder into water at 45-60 DEG C to obtain a high-molecular-weight sodium hyaluronate solution; cooling to 35-45 DEG C, adding a buffer solution, uniformly mixing, and then adding papain and hyaluronidase in a mass ratio of (2-3): (3-6) to carry out enzymolysis reaction, so as to obtain enzymatic hydrolysate; adding a sodium hydroxide solution into the enzymatic hydrolysate, adjusting the pH value to 7.5-9.0, heating to 80-90 DEG C, treating for 20-40 minutes, and filtering to obtain a low-molecular-weight sodium hyaluronate solution; and performing spray drying. The preparation method is simple in process, mild in condition and free of damage to the product structure, and the prepared low-molecular-weight sodium hyaluronate is high in purity, uniform in molecular weight distribution and good in permeability.

The invention discloses a high molecular weight poly (p-dioxanone) (PPDO) microsphere as well as a preparation method and application thereof. By adopting a double emulsification-solvent evaporation method, an amphiphilic polymer, such as poly (p-dioxanone) (PDO) or a copolymer of PDO and a hydrophilic fragment (such as mPEG and mPPG), is utilized to perform self-assembly on a two-phase interface formed by emulsification under the combined action of amphipathy and polyvinyl alcohol (PVA) to form a double-micelle protective layer, so that the degradation effect of water on PPDO in the microsphere forming process is effectively blocked, and the PPDO can be effectively degraded. And finally, the high-molecular-weight poly (p-dioxanone) microspheres with stable performance are obtained, so that the high-molecular-weight PPDO microspheres have long-term stability. The preparation method is high in operability, mild in condition and suitable for large-scale production, high-molecular-weight poly (p-dioxanone) microspheres are obtained, and the preparation method has wide application prospects in the aspects of tissue filling agents, drug carriers, tissue scaffolds and the like.

The invention discloses an ultra-high molecular weight polyethylene product dynamic compression molding process optimization method and system, and relates to the technical field of process processing, and the method comprises the following steps: determining a dynamic compression molding parameter design range; the dynamic compression molding parameters comprise molding parameters and product performance; adopting a Latin hypercube method to randomly generate sampling points required for constructing a response surface within the design range of the forming parameters; making a sample based on the sampling points, and testing the sample to obtain sample performance data; constructing a product performance response surface based on the sampling points and the sample performance data; and according to target product performance, performing optimization based on the product performance response surface to obtain an optimal parameter value. According to the method, performance optimal parameter selection of different products can be rapidly carried out by constructing the product performance response surface.

The invention discloses a preparation method and application of a salmon salmon sperm DNA sodium raw material, and belongs to the technical field of biomedicines.The preparation method comprises the following steps that S1, raw material pretreatment is conducted, specifically, after frozen salmon or salmon testis tissue is crushed at the ultralow temperature of-30 DEG C to-20 DEG C, a mixed solution containing a Tris-HCl buffer solution, NaOH and NaCl is added, and a mixed solution is obtained; adding a chitosan embedded compound enzyme with the total enzyme activity of more than or equal to 350KU / L, carrying out enzymolysis at 37 + / -1 DEG C for 26-30 hours, and maintaining the pH value to be 7.8-8.0, so as to prepare an enzymatic hydrolysate; s2, DNA synergistic crushing: adjusting the pH value of the enzymatic hydrolysate to 7.8, and in an ice-water bath of less than or equal to 10 DEG C, carrying out 25-35 kHz variable-frequency ultrasound and 100-120 MPa high-pressure homogenization alternate circulation for 3-5 times to carry out synergistic treatment for 40 minutes, so as to obtain a DNA crushing solution; the method solves the problems of high protein residue, non-uniform molecular weight distribution, low transdermal absorption rate, high cost, poor environmental protection property and the like in the traditional process extraction, and has the advantages of high product purity, high activity, low cost, greenness and environmental protection.

The invention relates to the technical field of cutter damage diagnosis, and discloses a fault diagnosis method and system for an ultra-high molecular weight polyethylene crushing device, and the method comprises the steps: obtaining a cutter impact contact image, recognizing an indentation region, extracting an indentation depth residual value and a rebound recovery height, and calculating an indentation rebound factor; collecting a material particle size distribution map, extracting a particle size main peak drift value, a perturbation particle proportion and a distribution full width at half maximum value, and constructing a particle size disturbance factor; performing difference analysis on the indentation springback factors and the particle size disturbance factors of the current batch and the adjacent batch, and weighting to construct an interference dynamic increment factor; and calculating an impact response deviation value based on the factor change trend, and judging whether the tool has a damage trend or a critical fault state or not. According to the invention, the tool wear trend and the critical fault state are identified and diagnosed.

The invention discloses an extraction method and application of high-molecular-weight turbellarian worm genome DNA, and belongs to the field of turbellarian worm genome extraction.The turbellarian worm genome DNA extraction is achieved by improving a lysate formula and removing ethylenediaminetetraacetic acid and increasing Mg < 2 + > on the basis of a conventional reagent in a classic Tris-HCl-phenol-chloroform method to inhibit activity of deoxyribonuclease II in a turbellarian worm body to inhibit degradation of genome DNA, and the high-molecular-weight turbellarian worm genome DNA is obtained. Meanwhile, ribonuclease A is added, RNA in cells can be degraded in the tissue cell digestion process, so that the step of two times of purification is omitted, the loss of DNA yield is reduced, experimental steps are simplified, especially the step of DNA purification is reduced, the problem of degradation in the extraction process of the turbellarian turbellarian worm genome DNA is solved, and the genome DNA yield (gt; 10 micrograms per turbellarian worm), and the turbellarian worm genome ultra-long sequencing or PacBio high-fidelity sequencing requirements are met.

Described herein are compositions and methods comprising high molecular weight hyaluronic acid (high MW HA). In some embodiments, high MW HA is formulated in combination with one or more peptides. In some embodiments, the peptides comprise an octapeptide with amino acid sequence features found in collagen and / or elastin.

The invention relates to the technical field of battery diaphragms, in particular to a ceramic diaphragm as well as a preparation method and application thereof. The material of the ceramic diaphragm provided by the invention comprises amino silicon dioxide, low-molecular-weight meta-aramid and high-molecular-weight meta-aramid. The meta-position aramid fiber with low molecular weight is coated on the surface of the amino silicon dioxide through a cross-linking agent; the meta-position aramid fiber with high molecular weight and the meta-position aramid fiber with low molecular weight are connected in a molecular winding manner; the viscosity average molecular weight of the meta-position aramid fiber with the low molecular weight is 1 to 7W; the viscosity average molecular weight of the meta-position aramid fiber with the high molecular weight is 10 to 20W. The ceramic diaphragm can obviously improve the cycling stability of the secondary lithium battery.

The invention discloses unimodal / bimodal distribution high-molecular-weight polyisobutene and a preparation method thereof, and belongs to the field of preparation of high-molecular-weight compounds. According to the preparation method, the high-molecular-weight polyisobutene with the molecular weight in unimodal / bimodal distribution is prepared through a simple initiator and co-initiator system. Using a simple and easily available initiator and Lewis acid with relatively weak corrosion to equipment as co-initiators, and carrying out simple initiator substituent group adjustment and technological process optimization at a relatively high temperature (-60 DEG C to-90 DEG C) to obtain the high molecular weight polyisobutene with the weight-average molecular weight range of 69.69 * 10 < 4 >-275.8 * 10 < 4 > g / mol and monomodal / bimodal distribution, wherein the high molecular weight polyisobutene has a weight-average molecular weight range of 69.69 * 10 < 4 >-275.8 * 10 < 4 > g / mol.

The invention relates to the technical field of chemistry and chemical engineering, and particularly discloses a high-molecular-weight bisphenol A type polycarbonate polyether polyol production process and a circulating flow rate cooperative control method, raw materials are premixed and then fed into a reaction pipeline at one time, gas-liquid circulation in opposite directions is performed in the reaction pipeline, and the high-molecular-weight bisphenol A type polycarbonate polyether polyol is obtained. Meanwhile, an intelligent industrial control technology is adopted for conducting time sequence collaborative analysis on the liquid circulation flow speed and the gas circulation flow speed, then characteristic screening optimization is conducted on gas-liquid circulation flow speed time sequence collaborative correlation characteristics based on the time sequence correlation change rule of the liquid circulation flow speed, and the optimal collaborative matching relation between the gas-liquid circulation flow speeds is captured; therefore, the self-adaptive adjustment of the liquid circulation flow velocity is carried out. Through the mode, cooperative work of the liquid circulating pump and the gas circulating pump can be realized, so that heat is conveniently dispersed in time, the temperature uniformity of a reaction system is maintained, and the problem that the reaction time is prolonged due to yellowing of a product caused by overhigh local temperature is avoided.

The present invention generally pertains to methods of characterizing crosslinking sites of a protein of interest. In particular, the present invention pertains to the use of size exclusion chromatography, peptide mapping and subunit analysis to identify and quantify crosslinking sites of a protein of interest and determine a contribution of crosslinking to the formation of high molecular weight species.

The invention discloses a CRISPR / Cas9 gene editing vector of a wheat TaMYB44 gene and application of the CRISPR / Cas9 gene editing vector, the vector takes pBUE414 as a skeleton vector and further comprises an sgRNA expression cassette and a Cas9 expression cassette, the Cas9 expression cassette is an expression cassette for regulating Cas9 expression by a Ubi promoter, and the sgRNA expression cassette sequentially comprises the following elements from upstream to downstream: a TaU3 promoter, sgRNA1 of TaMYB44, a terminator OsU3t, a TaU3 promoter, sgRNA2 of TaMYB44 and a terminator TaU3t. By utilizing the TaMYB44 gene knockout vector provided by the invention, TaMYB44 in wheat can be quickly knocked out, and the content of high-molecular-weight glutelin in obtained wheat material grains is remarkably increased.

The object of this invention is a lipophilic activity modifier in the form of dimethyl sulfone (MSM) for use as a transdermal penetration enhancer for high molecular weight active substances with a molecular weight (Mw) > 500 Da. Another object of this invention is a cosmetic composition for transdermal application comprising an active substance and a transdermal penetration enhancer, characterized in that the active substance is encapsulated by the transdermal penetration enhancer, which is the lipophilic activity modifier according to claim 1, and the active substance is a high molecular weight active substance with a molecular weight (Mw) > 500 Da. Another object of the present invention is a method for preparing a composition according to the invention, characterized in that the method is carried out by thermal emulsification or cold emulsification, wherein thermal emulsification comprises the following steps: a) mixing dimethyl sulfone and a diluent in a reaction vessel; b) bringing the mixture to 70-85°C; c) stirring the mixture at 70-85°C; the start of homogenization depends on the stage of phase combination; d) cooling the mixture to 20-40°C, preferably 30°C; e) adding a high molecular weight active substance with Mw>500 Da and homogenizing the mixture, preferably for 1-3 min; while cold emulsification comprises the following steps: a) mixing dimethyl sulfone and a diluent in a reaction vessel and dissolving at a temperature maintained at 20-25°C; b) bringing the mixture to a temperature of 15-35°C, preferably ambient temperature or 25°C; c) stirring the mixture at a temperature of 15-80°C, preferably 25°C; the start of homogenization depends on the stage of phase combination; d) adding a high molecular weight active substance with Mw>500 Da and homogenizing the mixture, preferably for 1-3 min.

The present invention relates to a high-molecular-weight hyaluronic acid structure obtained by applying shear stress to a solution containing high-molecular-weight hyaluronic acid, a method for preparing the same, and an ophthalmic composition including the same.

This invention relates to the fields of bioengineering and fermentation engineering, and discloses a genetically engineered *Streptomyces albopictus* strain that produces high levels of ε-polylysine and its fermentation production process. This genetically engineered strain uses *Streptomyces albopictus* strains acclimated to ε-polylysine tolerance as a chassis, and its genome integrates... ppc and dapf Through expression box, heterogeneous asd Expression cassettes, containing secretory signal peptides lysp Fusion expression cassettes and D404A / K499A point mutations pls Expression cassette. The fermentation process employs a two-stage pH control strategy, maintaining a neutral environment during the cell growth phase and adjusting to an acidic environment during product synthesis. This invention improves ε-polylysine yield through a synergistic strategy of enhancing precursor supply, constructing efflux detoxification channels, and increasing synthase activity; combined with an acidic fermentation process, it effectively inhibits product degradation, yielding a high-molecular-weight and highly uniform target product suitable for industrial production.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

A chemical mechanical polishing pad is provided containing a polishing layer having a polishing surface, wherein the polishing layer comprises a reaction product of ingredients, including: an isocyanate terminated urethane prepolymer; and, a curative system, containing a high molecular weight polyol curative; and, a difunctional curative.

The present invention concerns as methods of making compositions comprising Pertuzumab and variants of Pertuzumab. In particular, it concerns methods of making compositions comprising Pertuzumab and an unpaired cysteine variant comprising Cys23 / Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab. The compositions may further comprise an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The invention further concerns the methods of characterizing the variants and compositions thereof.

The application discloses a method for controllably degrading glycosidic bonds of hyaluronic acid by programmed temperature rising. The method is used for high-molecular-weight hyaluronic acid aqueous solution, and a combination of an oxygen oxidant and an acidic additive is used to controllably cut and degrade glycosidic bonds in the macromolecule through a heating process of programmed temperature rising control system, so as to obtain oligomeric hyaluronic acid. The oligomeric hyaluronic acid prepared by the method has very good stability and no color change after long-time storage. The oligomeric hyaluronic acid prepared by the method can be applied to the fields of cosmetics, food, medicine and the like, and has very strong practical value.

The invention relates to a rapid setting liquid lung sealant that forms a highly adherent and elastic hydrogel. Provided are lung sealants comprising two high molecular weight (20 kDa) multi-arm PEG compositions wherein the first PEG composition includes an alkaline buffer and the second PEG composition includes a mildly acidic buffer for optimized set up time and extended working time. In some embodiments, the PEGs further comprise a radioprotectant such as tocopherol. In some embodiments, a colorant is added to one or both PEGs of the lung sealant to improve visualization of the lung sealant against human lung tissue.

The invention provides a gene editing cell and a method for producing extremely high molecular weight hyaluronic acid by using the cell. The preparation method comprises the following steps: by taking PUC57 as a skeleton carrier, constructing a recombinant plasmid containing an HAS2 gene, and transfecting a mole NMRHas2 gene, a chicken CHICKHAS2 gene, an astronomy mouse SNMHAS2 gene, a dakalimeris mouse DMRHAS2 gene or a mole mouse BMRHAS2 gene into chicken fibroblasts through lipidosome to construct a stably transfected cell line, so that hyaluronic acid with extremely high molecular weight can be stably obtained. The method provided by the invention overcomes the defects of non-uniform molecular weight distribution of HA product sources, low molecular weight and single method in the prior art, and has important significance for developing medicines for treating osteoarthritis and novel medical equipment products.

Disclosed herein are peptide hydrogel-based formulations. In some embodiments, the peptide hydrogel-based formulations encapsulate antibodies. In some embodiments, the peptide hydrogel-based formulations prevent formation of high molecular weight protein species.

The invention relates to the technical field of medical preparations, in particular to moisturizing and repairing type artificial tear as well as a preparation method and application thereof. Comprising the following steps: preparing effective components including high-molecular-weight polydeoxyribonucleotide and hyaluronic acid or a salt thereof into a homogeneous-phase stable composite solution, sterilizing, filtering, and aseptically packaging, the artificial tear formed by selecting high-molecular-weight polydeoxyribonucleotide and high-molecular-weight sodium hyaluronate as effective components has a relatively good repairing effect on tissues, can form long-term moisturizing and lubrication on ocular surfaces, is suitable for treating xerophthalmia, repairing cornea, relieving inflammatory response and the like, and has a good application prospect. Important market values and clinical application prospects are realized.

An ultra-high molecular weight polyethylene (UHMWPE) polymer is provided having an average molecular weight of at least 2,000,000 g / mol and an enthalpy of at least 190 J / g. The UHMWPE polymer can include at least one comonomer. The UHMWPE polymer is used to form a membrane, which when stretched has a node and fibril structure. The UHMWPE membrane has an endotherm of about 150° C. associated with the fibrils in the membrane. The membrane has a porosity of at least 60% and a bubble point of 138 kPa or less. Additionally, the UHMWPE membrane has a thickness of less than 1 mm. The UHMWPE membrane can be formed by lubricating the UHMWPE polymer, subjecting the lubricated polymer to pressure at a temperature below the melting point of the UHMWPE polymer to form a tape, and stretching the tape at both temperatures below and above the melting temperature of the UHMWPE polymer.

The present invention provides a high sensitivity immunoassay method for determining the concentration of an analyte in a sample based on interferometry. The present invention provides a high molecular weight conjugate comprising a plurality of streptavidin and horse radish peroxidase (HRP) and using the conjugate to increase the sensitivity of biochemical assays. The present invention relates to a method for preparing a high molecular weight conjugate comprising at least 2 HRP, preferably 4 HRP, and binding a precipitated HRP substrate to a solid phase to increase the signal of a biochemical assay.

This application provides high molecular weight block copolymers comprising at least one block A comprising repeating units derived from monomers comprising lactam and acryloyl moieties and at least one block B comprising repeating units derived from hydrophilic monomers. The application further provides compositions comprising the block copolymers and applications thereof in various industrial areas including personal care. The application furthermore provides compositions comprising colloidal particles of the block copolymers. The variables x, y, R8 and R9 are described herein (I). Wherein each x and y is independently an integer having a value from about 10 to about 10000, and each R8, and R9 is independently selected from the group consisting of hydrogen, methyl, and combinations thereof.

The invention belongs to the field of chemical synthesis, and particularly relates to a chemical synthesis method of elastin-like polypeptide. The invention provides a chemical synthesis method of elastin-like, which comprises the following steps of: constructing a pentapeptide repetitive sequence (valine-proline-glycine-X-glycine, X is amino acid except proline, and the amino acid except glycine is respectively and independently selected from levoamino acid or dextroamino acid) of elastin-like polypeptide through liquid phase reaction; the elastin-like polypeptide can be prepared with the target molecular weight of 1000-500000 by adding amino groups and carboxyl groups into an organic solvent, forming orthogonal protection on the amino groups and the carboxyl groups, then selectively removing amino or carboxyl end protecting groups respectively, accurately coupling the amino groups and the carboxyl groups in a solution and repeating the deprotection and coupling processes for multiple times. The preparation method can realize accurate regulation and control of the molecular weight, sequence, chirality and topological structure of the elastin-like protein, improves the synthesis efficiency of the high-molecular-weight elastin-like polypeptide, has the advantages of large synthesis scale, low cost and the like, and has a wide application prospect.

The invention belongs to the field of implantable medical devices, and relates to a controllable molecular weight silk fibroin profile and a preparation method and application thereof, and the method comprises the following steps: adding cellulose derivatives into silk fibroin salt solutions with different molecular weights for swelling; in the silk fibroin salt solutions with different molecular weights, the narrow-distribution controllable high-molecular-weight silk fibroin salt solution has the index average molecular weight of 10 kDa to 80 kDa, 80 kDa to 100 kDa, 100 kDa to 120 kDa, 120 kDa to 160 kDa, 160 kDa to 200 kDa and more than 200 kDa. Filling the swelled silk fibroin salt solution into a dialysis bag with a specific shape and size, desalting in deionized water, and forming gel; finally, the obtained gel is taken out and slowly dried and shaped in a constant-temperature and constant-humidity environment, and the silk fibroin hard profile is obtained. The prepared silk fibroin profile has the advantages of being good in strength, green, non-toxic, easy to amplify and the like and can be further applied to the field of implantable medical devices, and the preparation method is high in efficiency, rapid in forming and more suitable for batch production and industrial production.