The invention provides a
mass-to-charge ratio-based
proton transfer reaction time-of-flight
mass spectrometer calibration method, and the method comprises the steps: S1, obtaining a function relation between the sensitivity of each standard gas group and the charge ratio of a corresponding
protonation product, each standard gas group independently comprises
organic matter gases belonging to the same category but different specific categories; s2, respectively substituting the
mass-to-charge ratios of the protonated products of different gas components in the gas to be detected into the function relationship of the respective corresponding standard gas group, and calculating to obtain the respective sensitivity of the different gas components in the gas to be detected; and S3, calculating the concentration of the gas component in the gas to be detected according to the respective
peak response intensity and sensitivity of different gas components in the gas to be detected. According to the method, the
reaction rate constant between the
volatile organic compound and the hydrated
hydrogen ions does not need to be obtained in advance, and the quantitative analysis efficiency of the
proton transfer reaction time-of-flight mass
spectrometer in a complex environmental sample is remarkably improved.