The invention relates to the technical field of
biology, and provides a
chip assembly and a preparation method thereof. The
chip assembly comprises a substrate, a circuit layer, a culture piece and a
microelectrode array. The
microelectrode array is located in the culture tank of the culture piece, the culture tank can be used for containing the to-be-monitored organ, and the to-be-monitored organ can make contact with the substrate and make contact with the
microelectrode array when located in the culture tank. The substrate and the microelectrode array are both flexible structural members, so that the substrate and the microelectrode array can deform. Therefore, when the
organoid is in the culture tank and the
organoid is in contact with the microelectrode array, the microelectrode array deforms to be matched with the appearance of the
organoid, so that the organoid is in more sufficient contact with the microelectrode array, the signals, collected by the microelectrode array, of the organoid are more accurate, and the damage of the microelectrode array to the organoid can be reduced. The base is also a flexible structural member, and the microelectrode array arranged on the base can move correspondingly by deforming the base so as to adjust the position of the microelectrode array.