198 results about "Polymicrobial Infections" patented technology

The invention relates to application of ten antibacterial peptides in the antimicrobial field. The ten antibacterial peptides have strong antibacterial activity on multi-drug-resistant bacteria, including but not limited to gram-negative bacteria such as escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, klebsiella pneumoniae and the like, and gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis and the like. Activity research proves that any one of the ten antibacterial peptides has a remarkable effect of inhibiting, killing or preventing microbial infection. Due to the strong antibacterial property of any antibacterial peptide, the antibacterial peptide has wide application potential in the aspects of preparing antibacterial drugs, preservatives, antibacterial coatings, antibacterial fibers, antibacterial packaging materials and the like. Due to the unique property of the antibacterial peptide in the aspect of antibacterial performance, a new direction is provided for the development of efficient and reliable antibacterial agents and antibacterial technologies and products in multiple fields.

The invention relates to the technical field of intelligent medical diagnosis, in particular to a clinical microbial infection intelligent diagnosis system and method based on multi-omics data fusion, and the method comprises the steps: collecting and processing time sequence multi-omics data, and generating a transient infection state snapshot vector; constructing a time sequence state transition model, inputting the instantaneous infection state snapshot vector into the time sequence state transition model, and predicting and generating a future state vector; respectively calculating a pathogen threat index and a host imbalance index based on the future state vector; generating a comprehensive risk score based on the pathogen threat index and the host imbalance index, and determining an early warning grade according to a preset early warning grade threshold; generating a hierarchical intervention strategy based on the early warning level in combination with the pathogen threat index and the host disorder index; outputting a grading intervention strategy as a specific diagnosis and treatment suggestion; according to the invention, prospective early warning is realized, and the situation that the optimal treatment opportunity is delayed due to the change of waiting indexes is effectively avoided.

In one aspect, nutraceutical compositions, methods of preparation and methods of use comprise a nutraceutical composition comprising an antimicrobial hevamine A-related protein from Momordica balsamina alone or in combination with one or more nutraceutical ingredients. In another aspect, a method of preventing or treating a microbial infection in a plant comprises applying an effective amount of a composition containing the hevamine A-related protein to a whole plant, plant part, or media in which the plant is growing. In a further aspect, the present application provides a transgenic plant stably transformed with a polynucleotide encoding the hevamine A-related protein.

The invention relates to low dose compositions and peptides or peptide sequences that induce an immune response in an animal or a mammal that is protective against infection by one or more pathogens, and the antibodies generated. In addition, the invention relates to immunogenic composition and vaccines comprising compositions and peptide sequences or antibodies, and to methods for treating and preventing an infection in animals and mammals such as humans.

The invention relates to a compound of formula Ia and / or formula Ib: or a pharmaceutically acceptable salt, solvate or prodrug thereof, where the groups are defined herein. The invention also relates to a pharmaceutical formulation comprising the compound for treating or detecting a microbial infection in a subject, a method of determining antimicrobial resistance of a microbial infection using the compound, and a method of determining an effective dose of one or more antimicrobial agents to kill a microorganism using the compound.

The invention relates to methods and compositions for preventing microbial infections. Methods and compositions for permanently affecting the microbial ecosystem (microbiome) in a subject to prevent infection and reduce recurrence of microbial infections are provided. In some embodiments, compositions and methods are provided for the production and use of molecularly modified bacterial strains having potential to prevent infection with a variety of microorganisms.

Electrically neutral complex compounds of formula I including any stereoisomer or E / Z isomer thereof wherein: M is Fe < 2 + >, Ru < 2 + >, Os < 2 + >, Co < 3 + >, Rh < 2 + >, Rh < 3 + >, Ir < 3 + >, Ni < 2 + >, Pd < 2 + >, Pt < 2 + > or Pt < 4 + >, in particular Ru < 2 + >; t and T'are H or (C1-C3)-alkyl; lig is a bidentate ligand, in particular 2, 2 '-dipyridyl (bpy) or 4, 7-diphenyl-1, 10-phenanthroline (BPhen); and A is an anion from a pharmaceutically acceptable acid, the electrically neutral complex compounds of formula I are useful in human therapy, in particular as photosensitizers (PS) in photodynamic therapy (PDT) of cancer, skin diseases, fungal infections or microbial infections. They are almost non-toxic under dark conditions at therapeutic doses and exhibit good to excellent photoactivity under hypoxic conditions. They can be used for PDT treatment of deep hypoxia tumors by using light within a far-infrared to near-infrared light treatment window.

The invention provides preparation of a PEG-modified antibacterial peptide and application of the PEG-modified antibacterial peptide in bacterial infection, belongs to the technical field of biomedical polypeptides, and particularly relates to preparation of the PEG-modified antibacterial peptide and application of the PEG-modified antibacterial peptide in a preparation for preventing or treating diseases related to bacterial infection. The PEG modified polypeptide designed in the invention is simple and convenient to operate, low in production cost and strong in antibacterial activity, can greatly optimize the toxicity and stability of the antibacterial peptide, can be used for preventing and treating diseases caused by various microbial infections, can be applied to various drug administration modes such as spraying, intraperitoneal injection, intravenous injection, nasal drop administration, spray administration and the like, and can be used for preparing drugs for preventing and treating diseases caused by various microbial infections. The traditional Chinese medicine composition has a good treatment effect on diseases such as pneumonia caused by skin wound infection, sepsis and bacterial infection.

The invention discloses design of an antibacterial peptide and application of the antibacterial peptide in bacterial infection, belongs to the technical field of biomedical polypeptides, and particularly relates to the antibacterial peptide designed through an amino acid replacement strategy and application of the antibacterial peptide in preparation of drugs for preventing or treating bacterial infection related diseases. The polypeptide designed in the invention has the advantages of short amino acid sequence, high efficiency and rapidness in preparation, strong antibacterial activity, wide antibacterial spectrum and weak red blood cell hemolysis activity, can be used for preventing and treating diseases caused by various microbial infections, and has a good treatment effect on diseases such as pneumonia caused by skin wound infection, sepsis and bacterial infection.

The invention provides a primer group, a kit and a method for simultaneously detecting 11 microorganisms and diagnosing chronic endometritis intrauterine infection. The primer group is used for detecting the existence of 11 chronic endometritis pathogens: chlamydia trachomatis, enterococcus faecalis, escherichia coli, trichomonas vaginalis, klebsiella pneumoniae, mycoplasma hominis, neisseria gonorrhoeae, staphylococcus, streptococcus, reproductive mycoplasma and ureaplasma urealyticum. The primer group is used for performing multiple joint detection on chronic endometritis related pathogens, has high specificity and sensitivity, and is adaptive to an endometrial tissue sample. The invention provides a method for simultaneously detecting 11 microorganisms for diagnosing chronic endometritis intrauterine infection, which comprises the following steps of: collecting an endometrial sample by adopting a disposable sterile endometrial sampler, detecting 11 microorganisms in the endometrial sample by adopting a multiplex PCR (Polymerase Chain Reaction) technology, and accurately detecting the intrauterine microbial infection condition. Meanwhile, more accurate technical support is provided for clinically identifying whether pathogenic bacteria causing chronic endometritis exist or not, and clinical doctors can comprehensively consider medication guidance conveniently.

Disclosed embodiments relate to wound care materials and methods that comprise a self-gelling composition, configured to form a gel upon contacting fluid. The self-gelling composition is a substantially homogeneous mixture, and comprises fluid-absorbent particles loaded with iodine-based antimicrobial agent, a dehydrated hydrogel powder, and a polymer base, configured to allow dispensing the self-gelling composition from a syringe or tube and to prevent the self-gelling composition from gelling prior to contacting fluid. The gel performs one or more of the following tasks during a wear time, when applied at the wound: absorbing fluid or exudate, releasing iodine-based antimicrobial agent, achieving antimicrobial activities at the wound, remaining structurally integral and cohesive, protecting the wound from exposure to microorganisms, and facilitating healing. After a wear time of at least three days, the gel can be removed in two pieces or less and leave less than 5% residue by weight.

The present application relates to the use of benzamide derivatives in pharmaceutical preparations for preventing and treating inflammatory injuries. The applicant has found that benzamide derivatives can be used as pharmaceutical preparations for preventing and / or treating cellular inflammatory damage caused by factors such as radiation or microbial infection. In some of the embodiments, it is demonstrated that benzamide derivatives have the effects of inhibiting cell pyroptosis, promoting the healing of radiation-complex injuries in mice, and reducing tissue inflammatory responses induced by the new coronavirus pseudovirus.

The invention provides a method of preparing a vaccine composition. The method includes infecting gene-edited porcine endogenous retrovirus (PERV) negative swine cells with a microorganism which expresses at least one protein antigen capable of inducing protective immunity in an animal against an infectious agent; culturing the infected cells in culture medium to propagate the microorganism; and harvesting the propagated microorganism from the culture medium to obtain a fraction comprising a PERV free antigen for use in immunizing an animal against the infectious agent.

The invention relates to fish scale polypeptide chelated zinc. The amino acid sequence of the fish scale polypeptide chelated zinc is HMGPR. According to the fish scale polypeptide chelated zinc disclosed by the invention, a polypeptide ligand HMGPR does not have antibacterial activity, but a nitrogen atom in a histidine imidazole group at an N end of the polypeptide ligand HMGPR is coordinated with Zn < 2 + > through lone pair electrons, and meanwhile, a carboxyl oxygen atom of arginine at a C end is used as an electron donor to form coordination with Zn < 2 + >, so that the fish scale polypeptide chelated zinc HMGPR (at) Zn is endowed with broad-spectrum antibacterial activity. Besides, HMGPR is chelated with Zn < 2 + > through a coordinate bond, so that the stability of the polypeptide is remarkably improved, and the defect that a peptidyl antibacterial agent is easily hydrolyzed by protease is overcome. The fish scale polypeptide chelated zinc can serve as a novel antibacterial preservative to be applied to the food industry, microbial infection is effectively prevented, and meanwhile the additional value and the comprehensive social value of the aquatic product industry are improved.

The invention provides a method of preparing a vaccine composition. The method includes infecting gene-edited porcine endogenous retrovirus (PERV) negative swine cells with a microorganism which expresses at least one protein antigen capable of inducing protective immunity in an animal against an infectious agent; culturing the infected cells in culture medium to propagate the microorganism; and harvesting the propagated microorganism from the culture medium to obtain a fraction comprising a PERV free antigen for use in immunizing an animal against the infectious agent.

The invention relates to the field of cell culture, and discloses a tumor complex microenvironment cell culture device which comprises a culture box, an observation assembly comprises a mounting groove formed in one side of the culture box, a sealing door is rotatably arranged in the mounting groove, a plurality of penetrating holes are formed in one side of the sealing door, and a plurality of through holes are formed in the other side of the sealing door. A plurality of sterile gloves are fixed to the side, close to the incubator, of the sealing door, the central axis of the penetrating hole and the central axis of the sterile gloves are located on the same straight line, a fixing opening is formed in one side of the sealing door, and an observation window is fixed in the fixing opening. In the invention, an experimenter can penetrate two arms into the sterile glove through the penetrating holes, then the experimenter can take out the culture mechanism on the fixed plate and get close to the observation window, and the growth condition of cells can be observed through the observation window supported by ultra-white glass, so that direct contact between the experimenter and the culture mechanism is avoided in the whole process; and the risk of microbial infection is reduced.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

The present invention relates to anti-CLEC12A antibodies and the antigen-binding fragments thereof. The present invention further relates to pharmaceutical compositions and diagnostic tests comprising the anti-CLEC12A antibodies or antigen binding fragments thereof, and the uses of such antibodies for the treatment and diagnosis of microbial infections.

The invention discloses a prefabricated photocatalyst film for retaining freshness of leaf vegetables as well as a preparation method and application of the prefabricated photocatalyst film. The photocatalyst film comprises the following components in parts by weight: 100 parts of chitosan; 0.5 to 2 parts of metal-nitrogen doped titanium dioxide; 0.5 to 1 part of riboflavin; 30 to 40 parts of glycerol; wherein the metal-nitrogen doped titanium dioxide is iron-nitrogen doped titanium dioxide and / or copper-nitrogen doped titanium dioxide. The photocatalyst film is used for sealing the prefabricated leaf vegetables, composite LED illumination in the cold chain distribution process is combined, the antibacterial and fresh-keeping effects are achieved, microbial infection of the prefabricated vegetables in the cold chain distribution process can be reduced, water loss and chlorophyll degradation can be reduced, and the shelf life of the prefabricated vegetables can be prolonged.

Provided herein are hydrogels that include a plurality of amine-containing antimicrobial agents within the interiors of the hydrogels. The antimicrobial agents are connected to the hydrogel interiors via dynamic covalent imine bonds, and the hydrogel is engineered to facilitate controlled release of the antimicrobial agents. Also provided are methods for forming the provided hydrogels, and for using the hydrogels to treat a subject suffering from a microbial infection.

The application relates to the technical field of biological medicine, and particularly discloses CpG oligodeoxynucleotides and application thereof. The CpG oligodeoxynucleotide has a sequence as shown in any one of SEQ ID NO. 3-6, 9, and preferably has a sequence as shown in SEQ ID NO. 3-6. The CpG oligodeoxynucleotide can effectively activate the immune system of a human body or an animal, has the functions of preventing and treating pathogenic microorganism infection in a broad spectrum, has the function of improving the immunogenicity of a common vaccine, and can be applied as a human or animal vaccine adjuvant.

The present invention provides stable antimicrobial and disinfectant compositions comprising use of a solid precursor of an oxidized state of chlorine. The invention also provides on-demand storage and mixing vessels and methods for preparing and delivering on demand formulations. In addition, the invention provides antiviral, antibiotic and general antimicrobial uses, in vivo, on surfaces and via spray applications.

The invention provides methods for treating or preventing microbial (eg, bacterial) infections and means for performing these methods. In particular, treatment of infections requiring rapid and durable therapy is made possible, such as for treating acute conditions such as septicemia, sepsis, SIRS or septic shock. The invention is particularly useful, for example, for treatment of microbes such as for environmental, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system. The invention also useful for the treatment of pathogenic bacterial infections in subjects receiving a treatment for a disease or condition, such as a transplant or a treatment for cancer, a viral infection or an autoimmune disease.

The present application relates to a compositions and methods comprising or expressing a hevamine A-related MoMo30 protein from Momordica balsamina. The MoMo30 protein is about 30 kDa in size, is stable after being autoclaved at 120° C. for 30 min, resists proteolytic cleavage by trypsin, exhibits mannose-sensitive binding to HIV gp120, exhibits hemagglutinin and chitinase activity, is capable of activating and stimulating T cell proliferation, is capable of preventing infection by HIV-1 or alleviating symptoms in an HIV-1 infected patients, and comprises an amino acid sequence of SEQ ID NO: 4. The MoMo30 protein and / or a nucleic acid encoding the same may be used in methods for preventing or treating microbial infections by HIV, SARS-CoV-2 and other enveloped viruses, as well as other microorganisms comprising cell surface proteins containing glycan residues, such as mannose.

Heterocyclic compounds with a novel pyrazole thioamide-based NNSN structural motif, having highly effective zinc- or copper-activated toxicity against microbial infections at micromolar or nanomolar minimum inhibitory concentrations (MIC), and methods of making and using the same.

The present invention relates to a complex for preventing, inhibiting, or treating microbial infections, and its preparation and use. The complex comprises an active portion, a binding portion, and a water-soluble portion. The active portion is a fat-soluble saturated and / or unsaturated carbon chain with a branched, cyclic, and / or linear structure that can insert into or integrate into the lipid membrane of microorganisms, disrupting the lipid membrane, or encapsulating non-enveloped viruses for hydrophobic isolation. The binding portion can bind to lipid membrane components or viral surface protein domains, allowing the complex to attach to the lipid membrane or viral surface. It can also have specific targeting lipid membrane components or viral surface protein domains to impart targeting. The water-soluble portion allows the complex to be uniformly dispersed in aqueous solutions and prevents aggregation of the active portion. The complex can be prepared into various dosage forms and can be used before infection to inhibit or prevent infection by viruses, bacteria, fungi, and other microorganisms; after infection, it can be used to kill microorganisms in the body; and it can also be used to disinfect the environment to prevent the spread of viruses, bacteria, and fungi.

The present disclosure allows for an anaerobic biofilm also known as a beneficial biofilm, to be grown on a hydrophilic substrate surface and contained within the varied surface and surface-accessible interior of the substrate. This allows for the anaerobic biofilm to remain intact and functional while being exposed to an aerobic environment. The present disclosure further allows for the constituent microbes to interact with an oxygen-rich surrounding without perishing. The present disclosure allows anaerobic biofilm constituent microbes to exist side-by-side with aerobic biofilm constituent microbes and employ various mechanisms to starve or eliminate unhealthy biofilms and sessile and free-floating unwanted microbes, and / or deliver substances to soil to improve soil health or to the body to assist in repair of injuries such as microbial infections, lesions, tumors, cuts, abrasions, burns, and nerve damage.

The invention discloses a tree frog antibacterial peptide as well as a gene and application thereof. The tree frog antibacterial peptide provided by the invention is a cyclic peptide consisting of 29 amino acids, the amino acid sequence of the tree frog antibacterial peptide is shown as SEQ ID NO.1, and the third cysteine and the seventh cysteine form an intramolecular disulfide bond. The invention also provides a nucleotide sequence for coding the tree frog antibacterial peptide, wherein the nucleotide sequence is shown as SEQ ID NO.2 or SEQ ID NO.9. The invention also provides a nucleotide sequence for coding the tree frog antibacterial peptide. The tree frog antibacterial peptide disclosed by the invention has very strong treatment functions of antibiosis, anti-inflammation, antioxidation and immunoregulation, has the characteristics of simple structure and convenience in artificial synthesis, and can be used for preparing medicines for treating pathogenic microorganism infection, autoimmune diseases and even sepsis.

A platform and method for testing antibiotic sensitivity of a polymicrobial infection is provided. The platform includes a body defining a plurality of sets of chambers and a plurality of wells. Each set of chambers has a plurality of chambers adapted for culturing microbes of the polymicrobial infection therein. Each well is associated with a corresponding set of chambers and has an input in fluidic communication with the outlets of the plurality of chambers in the corresponding set of chambers. Selected antibiotic therapies may be received in the wells which fluidically connect the plurality of chambers in a corresponding set of chambers such that microbes cultured in the plurality of chambers in the corresponding set of chambers are in soluble factor contact.

The invention discloses an antibacterial peptide as well as a composition and application thereof, an amino acid sequence of the antibacterial peptide is shown as a formula I. The antibacterial peptide has a good antibacterial effect on methicillin staphylococcus aureus MRSA, methicillin-sensitive staphylococcus aureus MSSA and methicillin-resistant staphylococcus epidermidis MRSE, and the antibacterial peptide has a good antibacterial effect on methicillin-sensitive staphylococcus aureus MRSA, methicillin-sensitive staphylococcus aureus MSSA and methicillin-resistant staphylococcus epidermidis MRSE. The antibacterial peptide and the pharmaceutical composition prepared by adding auxiliary materials are expected to be widely applied to preparation of drugs for controlling or preventing microbial infection.