Method for improving fermentation yield of hyaluronic acid

Abstract

The invention belongs to the technical field of hyaluronic acid preparation and particularly relates to a method for improving the fermentation yield of hyaluronic acid. Streptococcus zooepidemicus is taken as a hyaluronic acid fermentation strain; a reagent containing ferrous ions and copper ions is added to a culture medium used. The method provided by the invention is capable of improving the fermentation yield of hyaluronic acid by 30-34%, from 1.05 g / L to the range from 1.37 g / L to 1.41 g / L, and reducing the cost by 30%; meanwhile, the method provided by the invention has no influence on the molecular weight and quality of a product.

Description

technical field [0001] The invention belongs to the technical field of hyaluronic acid preparation, in particular to a method for improving the fermentation yield of hyaluronic acid. Background technique [0002] Hyaluronic acid is hyaluronic acid (Sodium Hyaluronate), hyaluronic acid, referred to as HA, is an acidic mucopolysaccharide. Its molecular structure is formed by repeatedly connecting N-acetylglucosamine and glucuronic acid through β-1,4 and β-1,3 glycosidic bonds, and its molecular weight is between 100,000 and 4,000,000. Two monosaccharides in the molecule, namely β-D-glucuronic acid (glucuronic acid) and N-acetylamino-D-glucosamine (N-acetyl glucosamine), are composed in a molar ratio of 1:1. Molecular formula: (C 14 h 20 NNaO 11 )n, CAS number: 9004-61-9, its disaccharide structure is as follows: [0003] [0004] Molecular structure of HA [0005] The biosynthetic route of HA shows that hyaluronic acid is a disaccharide monosaccharide linked by glucur...

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Problems solved by technology

Such as Chinese patent CN101096632A has reported a kind of novel reactor that is used for hyaluronic acid fermentation, has improved air distributor and stirring paddle, but because the diameter of solid disk stirring paddle is too large, shearing force is too big to easily interrupt hyaluronic acid Molecules, reduced molecular weight, resulting in limited application of the product in surgical procedures

The second is to add oxygen carriers. Patents CN200810123397.8 and CN200910161252.1 respectively report a method for increasing the yield of hyaluronic acid by adding oxygen carriers n-dodecane and n-hexane, respectively, which increase the fermentation yield by 10% and 30%, but the alkanes are not easy to remove in the subsequent extraction steps, resulting in a decline in product quality

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