Method for improving pristinamycine fermentation output

Abstract

The invention discloses a method for improving the Pristinamycin (also named Peristamycin)output, in which the Pristinamycin is the fermentate of Streptomyces pristinaespiralis. By directly adding the macroreticular resinous adsorbent into fermentation liquor, the in-situ linkage of microorganism fermentation and product segregation can be carried out, which alleviates the Pristinamycin relaxation for production bacterium at a large degree and simultaneously avoids the probability that the Pristinamycin can be easily degraded in the later fermentation. By selecting macroreticular resinous adsorbent species, adding quantity, adding time and adding mode, the fermentation level of the said Pristinamycin can be increased to 1.0-2.5g / L, which can effectively improve the Pristinamycin output, accordingly it can reduce the Pristinamycin cost of manufacture.

Description

technical field

[0001] The invention relates to a production process of pinamycin, a fermentation product of Streptomyces initiating spiral, and belongs to the field of microbial engineering, in particular, it relates to a method for increasing the fermentation yield of pinamycin. Background technique

[0002] The long-term use of antibiotics, especially the irrational use, has made clinical pathogenic bacterial infection a very serious problem in the medical field. It is very urgent to research and develop antibiotics with the ability to resist drug-resistant bacteria. Pristinamycin (Pristinamycins, also known as primitive mycin), a kind of streptoxin antibiotic produced by the fermentation of Streptomyces pristinaespiralis, is such an antibiotic product, and it is effective against Gram-positive bacteria. Good antibacterial ability, not easy to produce drug resistance. Prinamycin is composed of about 30% of punamycin I (by P 1A ,P 1B and P 1C Composition) and about 70%...

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