Method for improving pristinamycine fermentation output

A technology of punamycin and production, which is applied in the field of microbial engineering, can solve the problems of high production cost and difficulty in further improving the fermentation level of punamycin, and achieve the effects of reducing production cost, increasing production and alleviating inhibition

Inactive Publication Date: 2006-12-13
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above reasons make it difficult to further improve the fermentation level of prunamycin, so the production cost remains high

Method used

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  • Method for improving pristinamycine fermentation output
  • Method for improving pristinamycine fermentation output

Examples

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Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: add macroporous adsorption resin fermentation production prunamycin new technology in shake flask

[0023]The cultivated mature Streptomyces spore culture was inoculated into an Erlenmeyer flask containing 25 milliliters of seed medium with the method of digging (the size of the digging block was 1 × 1 square centimeter), placed in a shaker for shaking and culturing for 36 hours, The rotating speed of the shaker was set at 220 rpm, and the culture temperature was 28°C. When the concentration of the mycelia of Streptomyces involutes in the seed liquid reached 20%, the culture was terminated. Inoculate 1.5 milliliters of seed liquid into an Erlenmeyer flask containing 25 milliliters of fermentation medium, place it in a shaker for shaking culture, set the speed of the shaker at 250 rpm, and the culture temperature at 28°C. hour, directly add the sterilized HZ-802 macroporous adsorption resin into the fermentation broth, the amount of resin added is 2.1g wet ...

Embodiment 2

[0024] Embodiment 2: in 5 liters of fermentors, add macroporous adsorption resin and ferment and produce prunamycin new technology

[0025] 180 milliliters of seed liquids are inoculated into 5 liters of fermentors containing 3 liters of fermentation medium, and fermented. In the fermentors, feed sterile compressed air with a ventilation rate of 1.2 liters / (min × liters), and a stirring speed of 200 rev / min), the temperature is controlled at 28°C. When the fermentation was carried out to the 18th hour, the sterilized HZ-802 macroporous adsorption resin was added to the fermenter by flame method, and the amount of resin added was 150 grams of wet weight; then when the fermentation continued to the 25th hour , and then add 150 grams of HZ-802 wet resin by flame method, and ferment until the end of the 63rd hour. Filter and isolate the adsorption resin in the fermented liquid, use 1200 milliliters of concentration as the acetone solution of 70% to elute twice step by step, combi...

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Abstract

The invention discloses a method for improving the Pristinamycin (also named Peristamycin)output, in which the Pristinamycin is the fermentate of Streptomyces pristinaespiralis. By directly adding the macroreticular resinous adsorbent into fermentation liquor, the in-situ linkage of microorganism fermentation and product segregation can be carried out, which alleviates the Pristinamycin relaxation for production bacterium at a large degree and simultaneously avoids the probability that the Pristinamycin can be easily degraded in the later fermentation. By selecting macroreticular resinous adsorbent species, adding quantity, adding time and adding mode, the fermentation level of the said Pristinamycin can be increased to 1.0-2.5g / L, which can effectively improve the Pristinamycin output, accordingly it can reduce the Pristinamycin cost of manufacture.

Description

technical field [0001] The invention relates to a production process of pinamycin, a fermentation product of Streptomyces initiating spiral, and belongs to the field of microbial engineering, in particular, it relates to a method for increasing the fermentation yield of pinamycin. Background technique [0002] The long-term use of antibiotics, especially the irrational use, has made clinical pathogenic bacterial infection a very serious problem in the medical field. It is very urgent to research and develop antibiotics with the ability to resist drug-resistant bacteria. Pristinamycin (Pristinamycins, also known as primitive mycin), a kind of streptoxin antibiotic produced by the fermentation of Streptomyces pristinaespiralis, is such an antibiotic product, and it is effective against Gram-positive bacteria. Good antibacterial ability, not easy to produce drug resistance. Prinamycin is composed of about 30% of punamycin I (by P 1A ,P 1B and P 1C Composition) and about 70%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 金志华雷引林贾波李宁惠陶正利吴亚铭梅乐和
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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