Method of increasing yield of spinosad by improving fermentation condition of saccharopolyspora spinosa based on metabonomics

A technology of Saccharopolyspora spinosyn and spinosad, which is applied in the field of improving the fermentation conditions of Saccharopolyspora spinosa to increase the yield of spinosad based on metabolomics, and can solve the problems such as unclear metabolic mechanism
CN104450834AInactive Publication Date: 2015-03-25TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2015-03-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method of increasing the yield of spinosad by improving the fermentation condition of saccharopolyspora spinosa based on metabonomics. The method comprises the following steps: (1) determining intracellular metabolite of the saccharopolyspora spinosa; (2) analyzing the capability of the saccharopolyspora spinosa of producing the spinosad; (3) carrying out PLS analysis; and (4) carrying out process analysis. According to the method disclosed by the invention, the metabolite change law in the process of producing the spinosad by virtue of fermentation of the saccharopolyspora spinosa is revealed by utilizing the metabonomics means and combining multivariate statistics, and a metabolic change mechanism related to production of the spinosad is further explained; by analyzing the spinosad production capability and the metabolic level of the saccharopolyspora spinosa in different growth environments, the metabolin related to the production of the spinosad is found, thus providing a direction for optimizing the culture process of the saccharopolyspora spinosa and increasing the fermentation yield of the spinosad. The invention also provides new thinking and method for study in other culture technological processes of producing macrolides microorganisms.
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Description

technical field

[0001] The invention belongs to the field of biological pesticides, and in particular relates to a method for improving the fermentation conditions of Saccharopolyspora spinosa based on metabolomics to increase the yield of spinosad. Background technique

[0002] Spinosyn, also known as spinosyn or spinosyn, is a macrolide antibiotic produced by secondary metabolism of Saccharopolyspora spinosa under aerobic fermentation conditions, mainly including spinosyn A (approx. 85%-90%) and spinosyn D (about 10%-15%) two compounds. Spinosyn, as a high-efficiency broad-spectrum macrolide insecticidal active substance, has a unique mode of action, wide insecticidal spectrum, short half-life, high insecticidal activity, no drug resistance, low residue, less environmental pollution and Some unique physical and chemical properties, biological characteristics and novel mechanism of action, such as being harmless to humans and animals, are called a new generation of broad-s...

Claims

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