Method of improving byaluronic acid yield and molecular weight by fermentation process

A technology of hyaluronic acid and molecular weight, which is applied in the field of fermentation of hyaluronic acid production, can solve the problems affecting the quality of hyaluronic acid products, the yield of hyaluronic acid and the low molecular weight, etc., so as to improve the yield and molecular weight, reduce production costs, improve Effects of fermentation methods

Inactive Publication Date: 2006-06-14
JIANGNAN UNIV
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Problems solved by technology

The production of hyaluronic acid by traditional fermentation method mostly uses yeast powder and beef extract as nitrogen source at the same time, and adopts high stirring speed, the yield and molecular weight of hyaluronic acid obtained are low, which affects the quality of final hyaluronic acid product

Method used

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Embodiment 1

[0009] Using Streptococcus zooepidemicus H23 as the starting strain, hyaluronic acid is produced by liquid fermentation, the fermentation medium selects 8.0-12.0g / L yeast powder as a single nitrogen source, and the fermentation medium consists of: glucose 65.8g / L, Yeast powder 8.0~12.0g / L, Na 2 HPO 4 ·12H 2 O6.2g / L, K 2 SO 4 1.3g / L, MgSO 4 ·7H 2 O2.0g / L, FeSO 4 ·7H 2 O0.005g / L; the process conditions are: temperature 37°C, ventilation rate 1.2vvm, the stirring speed of the fermentation process is about 200rpm, the yield of the obtained hyaluronic acid reaches 6.0g / L, and the molecular weight reaches 2.00×10 6 Da.

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Abstract

The invention relates to a method of improving fermentation method producing hyaluronic acid yields and molecular weight. It belongs to hyaluronic acid fermentation technique field. Its steps is as follows: adopting streptococcus zooepidemicus H23 as start strain; producing hyaluronic acid by liquid fermentation; fermenting culture medium and using yeast powder as single nitrogen source; the yields and molecular weight of the hyaluronic acid is greatly increased at 180-220rpm rotate speed. It is applied to hyaluronic acid industrialization production. The invention can increase fermentation method producing hyaluronic acid yields and molecular weight, economize energy consumption, reduce production cost, and improve hyaluronic acid production quality.

Description

technical field [0001] The invention relates to a method for improving the yield and molecular weight of hyaluronic acid produced by a fermentation method, and relates to the technical field of fermentation for producing hyaluronic acid. Background technique [0002] Hyaluronic acid (HA), also known as hyaluronic acid or hyaluronic acid, is a macromolecular mucopolysaccharide that widely exists in the connective tissue of living organisms. The physiological role of HA in different tissues is different, for example, in the skin, it is mainly water retention; in the synovial fluid, it is mainly lubricating; in the blood vessel wall, it mainly regulates the permeability. In addition, as a polyanion electrolyte, HA has a large number of negative charges on the molecule, which can adjust the concentration of surrounding positive and negative ions and inhibit the activity of various enzymes. It is widely used in ophthalmic viscous surgery, treatment of joint diseases, soft tissue ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/08
Inventor 陈坚成霞刘登如堵国成
Owner JIANGNAN UNIV
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