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Production technology for fermentatively producing sodium hyaluronate by utilizing bacterium

A technology of sodium hyaluronate and production process, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low yield, unsuitable for large-scale production, high cost, etc., and achieve the effect of high mutagenesis efficiency

Inactive Publication Date: 2013-06-26
宁波林叶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, wild hyaluronic acid production strains generally have low yields, and the resulting high cost makes them unsuitable for large-scale production. Therefore, we comprehensively adopted a variety of mutagenesis methods to conduct a wild strain of hyaluronic acid production. Preferably, a high-yielding bacterium is finally obtained, and its yield can reach up to 10g / L, thus greatly reducing the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Take the mutant strain SY-121 as the starting bacterium, take one freeze-dried bacterial powder, dissolve the bacterial powder with 2ml of sterile normal saline, use the inoculation loop to take the bacterial solution and inoculate it on the slant medium, and culture it at 32°C for 24 hours, Inoculate it in 1.6L primary seed medium, shake culture at 32°C for 18h, then inoculate it in 80L secondary seed medium, culture with ventilation and stirring at 32°C for 10h, then transfer to 0.8t fermentation medium, fermentation medium The ingredients before sterilization are: yeast powder 10g / L, Na 2 HPO 4 5g / L, MgSO 4 5g / L, defoamer 1.5g / L. After the sterilization is completed, transfer 16L of 500g / L sterile glucose solution prepared in advance to the fermenter, then add 80L of seed solution, start automatic cultivation, use 10N NaOH to control the pH of the fermentation solution at about 7.2, and control the temperature At 32.5°C, the stirring speed is 150rev / min for 0~4h...

Embodiment 2

[0046] Take the mutant strain SY-121 as the starting bacterium, take one freeze-dried bacterial powder, dissolve the bacterial powder with 2ml of sterile normal saline, use an inoculation loop to take the bacterial solution and inoculate it on the slant medium, and culture it at 33°C for 24 hours. Inoculate it in 1.6L primary seed medium, shake culture at 33°C for 12 hours, then inoculate it in 80L secondary seed medium, culture with ventilation and stirring at 33°C for 10h, transfer to 0.8t fermentation medium, and ferment medium The ingredients before sterilization are: yeast powder 25g / L, Na 2 HPO 4 3g / L, MgSO 4 3g / L, defoamer 1.5g / L. After the sterilization is completed, transfer 16L of 500g / L sterile glucose solution prepared in advance to the fermenter, then add 80L of seed solution, start automatic cultivation, use 10N NaOH to control the pH of the fermentation solution at about 8.0, and control the temperature At 33°C, the stirring speed is 150rev / min for 0~6h, 20...

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Abstract

The invention relates to a production technology for fermentatively producing sodium hyaluronate by utilizing bacterium. The production technology comprises the following steps of: using streptococcus equi subsp. zooepidemicus (Streptococcus Zooepidemicus) as an original strain; mutagenizing by virtue of nitrosoguanidine, and also mutagenizing by virtue of a protoplast in the presence of ultraviolet rays; introducing a special culture medium; carrying out submerged fermentation to produce high-output hyaluronic acid; and then carrying out a relative separation and purification technology to obtain a high-purity sodium hyaluronate product. According to the production technology for fermentatively producing the sodium hyaluronate by utilizing the bacterium, under the culture conditions that the culture temperature is 32 to 38 DEG C, the pH (Potential Of Hydrogen) of fermentation liquor is maintained within the range of 5.0 to 9.0, and a fermentation tank is at speed of stirring of 150 to 600rev / min, the output of the hyaluronic acid is greatly increased; and the production technology is suitable for being applied to industrial production.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation of sodium hyaluronate, in particular to a production process for producing sodium hyaluronate by bacterial fermentation. Background technique [0002] Hyaluronic acid is a macromolecular acidic mucopolysaccharide with unique molecular structure and physical and chemical properties widely present in animal connective tissue and some microbial capsules. Because of its high viscoelasticity and strong water retention, it can act as a Such as lubricating joints, regulating the permeability of blood vessel walls, regulating the diffusion and operation of protein, water, and electrolytes, promoting wound healing, improving skin nutrition metabolism, making skin soft, smooth, wrinkle-free, increasing elasticity, and preventing aging. Has been widely used in clinical medicine, pharmaceutical and cosmetic industries. [0003] Early hyaluronic acid was obtained from animal tissues such as hum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/46
Inventor 杨晓辉叶昀林克王洪森
Owner 宁波林叶生物科技有限公司
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