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Method for producing high molecular weight sodium hyaluronate through fermentation and culture medium utilized by same

A technology of sodium hyaluronate and fermentation medium is applied in the field of fermentation and production of high molecular weight sodium hyaluronate, which can solve the problems affecting HA yield, quality production cost, molecular weight loss, etc., so as to prevent the loss of yield and molecular weight, and reduce the content of HA. , the effect of reducing costs

Inactive Publication Date: 2011-11-16
SHANGHAI INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the complex extraction process, HA molecules will be affected by various physical and chemical means, and the molecular weight will be greatly lost, which directly affects the yield, quality and production cost of HA.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Adopt the compound amino acid liquid culture medium 500mL shake flask of the present invention to ferment and produce HA-Na:

[0094] The ratio of the amino acid compound solution in the medium is (g / L)

[0095] Alanine 3.4 Arginine 2.0 Aspartic Acid 4.0

[0096] Cystine 4.0 Glutamic acid 0.4 Glycine 0.6

[0097] Histidine 1.1 Isoleucine 2.1 Tyrosine 2.2

[0098] Leucine 3.1 Lysine 3.2 Methionine 1.0

[0099] Phenylalanine 1.8 Proline 1.5 Threonine 2.0

[0100] Tryptophan 0.8 Valine 1.7 Serine 2.1

[0101] Glutamine 0.7 Uracil 0.6 Aspartic Acid 0.8

[0102] The glycerol tubes of Streptococcus zooepidemicus stored in the refrigerator at -86°C were thawed through gradients, and an appropriate amount was picked and inoculated on the slant medium under aseptic operation, and cultured at 37°C for 16 hours to finally obtain the slant strain zooepidemicus cocci;

[0103] Inoculate the slant strains into the seed medium, cultivate at 35°C and 300rpm for 10 hours, and th...

Embodiment 2

[0107] Adopt the compound amino acid liquid culture medium 500mL shake flask of the present invention to ferment and produce HA-Na:

[0108] The ratio of the amino acid compound solution in the medium is (g / L):

[0109] Alanine 2.4 Arginine 1.5 Aspartic Acid 3.5

[0110] Cystine 0.35 Glutamic acid 0.57 Glycine 1.3

[0111] Histidine 0.7 Isoleucine 1.3 Tyrosine 1.0

[0112] Leucine 1.5 Lysine 2.0 Methionine 0.2

[0113] Phenylalanine 1.0 Proline 1.0 Threonine 1.0

[0114] Tryptophan 0.1 Valine 1.0 Serine 1.5

[0115] Glutamine 0.3 Uracil 0.2 Aspartic Acid 0.2

[0116] The cultivation and extraction methods were the same as in Example 1, and the quality of the obtained HA-Na product was tested, and the results are shown in Table 1 below.

Embodiment 3

[0118] Adopt the compound amino acid liquid culture medium 500mL shake flask of the present invention to ferment and produce HA-Na:

[0119] The ratio of the amino acid compound solution in the fermentation medium is (g / L):

[0120] Alanine 4.4 Arginine 2.5 Aspartic Acid 4.5

[0121] Cystine 0.55 Glutamic acid 0.77 Glycine 3.3

[0122] Histidine 1.7 Isoleucine 3.3 Tyrosine 3.6

[0123] Leucine 4.5 Lysine 4.5 Methionine 1.3

[0124] Phenylalanine 2.5 Proline 2.5 Threonine 3.0

[0125] Tryptophan 1.5 Valine 3.5 Serine 3.5

[0126] Glutamine 1.0 Uracil 1.0 Aspartate 1.0

[0127] The cultivation and extraction methods were the same as in Example 1, and the quality of the obtained HA-Na product was tested, and the results are shown in Table 1 below.

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PUM

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Abstract

The invention discloses a novel method for producing high molecular weight sodium hyaluronate (HA-Na) with amino acid complex liquid as a nitrogen source through fermentation. In the method for producing the high molecular weight sodium hyaluronate, provided by the invention, yeast cream or yeast powder is replaced with the amino acid complex liquid as a fermentation nitrogen source of streptococcus zooepidemicus. The HA-Na with molecular weight of 2.2*10<6>D is obtained through control of a fermentation process. When the method is applied to industrial production, on the one hand, contents of hetero-protein and pigment in fermentation liquid can be effectively reduced, the step of subsequent HA-Na extraction is decreased, and the physical damage of the molecular weight of HA-Na in the fermentation liquid in the extraction process is reduced; on the other hand, the HA-Na production cost can be lowered, and the novel method has higher industrial application value. The HA-Na produced by the method has the molecular weight of 2.0-2.2*10<6>D, the alduronic acid content of 42.4-43.5%, the intrinsic viscosity of 3,064-3,115 and the protein content of 0.1%.

Description

technical field [0001] The invention relates to a method for fermenting and producing high molecular weight sodium hyaluronate and a culture medium used therein. That is, the method of producing high molecular weight sodium hyaluronate by fermenting the fermentation medium using the compound amino acid liquid as the nitrogen source. Background technique [0002] Hyaluronic acid (Hyaluronic acid, Hyaluronan, hereinafter referred to as HA), also known as hyaluronic acid, is (1→3)-2-acetylamino-2-deoxygen linked by β-1,3 and β-1,4 glycosidic bonds -β-glucose (1→4)-O-β-D-glucuronic acid disaccharide repeating unit composition, which widely exists in the connective tissue of organisms. The physiological role of HA is different in different tissues, such as water retention in the skin; lubrication in the synovial fluid of the joints; permeability regulation in the blood vessel wall. In addition, as a polyanionic electrolyte, HA has a large number of negative charges on the mole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/46
Inventor 荣绍丰管世敏侯永泰陈奕涵丁保妹
Owner SHANGHAI INST OF TECH
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