2 results about "Streptococcus uberis" patented technology

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

The invention relates to the technical field of biology, and particularly discloses streptococcus suis lyase Ply2413 and application thereof. Wherein the amino acid sequence of the lyase is as shown in SEQ ID NO: 1, or the lyase is a functional variant which has the same CHAP catalytic structural domain and SH3b binding structural domain as the lyase and retains broad-spectrum splitting activity. The lyase can be prepared from the coding gene of the lyase through a recombinant expression system. The preparation method comprises the following steps: cloning a gene for coding the lyase, constructing a recombinant expression vector, transforming host cells, carrying out induced expression, purifying to obtain target lyase, and further preparing various antibacterial products. The streptococcus suis lyase Ply2413 disclosed by the invention can be used for preventing and controlling streptococcus suis, staphylococcus aureus and streptococcus uberis infection, and has the advantages of wide lysis spectrum, multiple action targets and good environmental stability; in addition, the preparation method disclosed by the invention has the advantages of simplicity and convenience in operation, high expression efficiency, large-scale production and high technical repeatability.