Method for quickly detecting pathogenic bacteria in cow endometritis

Abstract

The invention discloses a method for quickly detecting pathogenic bacteria in cow endometritis. The method comprises the following steps of (1) extracting DNA of 6 common pathogenic bacteria which arecultured over night, in womb secretion by a reagent kit method; (2) in a reaction system containing the DNA of the bacteria, adding a PCR primer system which can detect the specificity sequence of the 6 kinds of bacteria at the same time, and performing amplification; and (3) preparing 2% of agarose gel, performing gelatin electrophoresis, wherein the voltage is 120 V and the time is 45 min, andthen performing electrophoresis ultraviolet imaging. Based on a multiple polymerase chain reaction technique, the method disclosed by the invention can realize detection of 6 kinds of the bacteria, including E.coli, S.aureus, T.pyogenes, S.uberis, P.mirabilis and P.aeruginosa. Compared with sequencing, techniques of substance PCR and the like are simpler and more convenient to operate, lower in cost, higher in sensitivity and specificity. The detection and identification period for clinical bacterium detection are selected.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to a method for rapidly detecting pathogenic bacteria in cow endometritis. Background technique [0002] Cow endometritis (endometritis) is a disease of cows caused by pathogenic microorganism infection and other factors to cause inflammation of the endometrium, resulting in pathological changes in the endometrium layer. Most of the disease occurs during parturition or postpartum of dairy cows, and it is the most common reproductive disorder of dairy cows. Infertility caused by endometritis can account for 60.0%-70.0% of disease-induced infertility. The disease can lead to repeated infertility and even death of affected cows, which has a long-term irreversible impact on the reproductive system of dairy cows, resulting in increased feeding costs, decreased milk production, and even increased culling rates, which have brought serious problems to dairy farms. serious economic lo...

AI Technical Summary

Problems solved by technology

PCR-DGGE technology can analyze a large number of samples at the same time, quickly and accurately identify individual microorganisms, and has the advantages of high resolution and small sample demand, but this technology also has certain limitations: this technology can only detect DNA fragments below 500bp, And it is difficult to detect microorganisms with a content of less than 1%, and co-migration is prone to occur during electrophoresis

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