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Trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection

A metal-organic framework and aptamer sensor technology, which is applied in the field of electrochemical detection, can solve the problems of antibody instability and limited use, and achieve the effects of promoting precision medicine, facilitating commercialization, and simple methods

Active Publication Date: 2019-05-10
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

[0004] Due to the instability of traditional biorecognition molecules (eg, enzymes, antibodies), their use in biosensors is limited

Method used

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  • Trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection
  • Trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection
  • Trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection

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Embodiment 1

[0039] Step 1. First, 4.34g of Ce(NO 3 ) 3 ·6H 2 O was added to 45 mL of ultrapure water to dissolve to form solution A. Then use 10mL water-ethanol (1:1) as solvent, add 2.10g of H 3 BTC, forming solution B. Solution A was gradually added dropwise to solution B at 60° C. under vigorous stirring (800 rpm), and fully reacted for one hour. Centrifuge (8000rpm), wash several times with ethanol and ultrapure water to obtain Ce-MOF, and dry it for future use. Subsequently, the nanocomposite Ce-MOF@Au was prepared by first weighing 1 mg of Ce-MOF and dispersing it in 2 mL of ultrapure water, and then adding 2 mL of 2% HAuCl 4 , after mixing well, add 2mL of PVP solution (2mg mL -1 ), and ultrasonically mix for 15 min. Stirring at 800rpm, slowly drop 2mL NaBH 4 (7.5mg mL -1 ), continued to stir at room temperature for 30 min, centrifuged, and washed three times with ultrapure water to obtain the Ce-MOF@Au nanocomposite, which was dispersed in Milli-Q water.

[0040] Step 2....

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Abstract

The invention successfully develops a sensor for the sensitive detection of thrombin sensitive protein-1 (TSP-1) based on the signal amplification strategy of a cerium metal organic framework @gold nanocomposite (Ce-MOF@Au) and gold-platinum-ruthenium nanocomposite (AuPtRu NP) using an aptamer as a target capture agent. The synthesized AuPtRu NP can be used not only as a catalyst for catalyzing hydrogen peroxide, but also as a nanocarrier to capture an amino group (-NH2) to terminate a single-stranded DNA (S1) to obtain a signal probe SP (AuPtRu NP / S1). Ce-MOF@Au is obtained by in-situ reduction and used as an electrode modification material of a glassy carbon electrode. When the detection solution contains an electrochemical matrix containing H<2>O<2>, AuPtRu NP can oxidize H<2>O<2> to obtain an enhanced signal. The invention shows a very low detection limit of 0.13 fg mL<-1> in the linear range from 1 fg mL<-1> to 10 ng mL<-1>. In addition, the trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection is shown to be directly usable for the detectionof clinical actual samples.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical aptasensor for quantitatively detecting thrombin-1 in clinical practice, especially based on cerium metal-organic framework@gold nanocomposite (Ce-MOF@Au) and gold A platinum-ruthenium nanocomposite material (AuPtRu NP) is used as a biosensor prepared as a signal probe to detect thrombin-1, which belongs to the field of electrochemical detection. Background technique: [0002] Cardiovascular disease (CVD) is a disease of the heart and blood vessels and is the leading cause of death worldwide. Individuals at risk for CVD may exhibit increased levels of biomarker molecules in the blood. Identification of biomarkers of highest CVD risk to aid in diagnosis could prevent premature death. Thrombospondin-1 (TSP-1), a promising candidate biomarker for CVD, is highly expressed in large atherosclerotic lesions and myocardial infarction. Therefore, a series of methods, such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
Inventor 于超付晓雪何俊琳
Owner CHONGQING MEDICAL UNIVERSITY
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