Trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection

A metal-organic framework and aptamer sensor technology, which is applied in the field of electrochemical detection, can solve the problems of antibody instability and limited use, and achieve the effects of promoting precision medicine, facilitating commercialization, and simple methods
CN109738495AActive Publication Date: 2019-05-10CHONGQING MEDICAL UNIVERSITY
5 Cites 17 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHONGQING MEDICAL UNIVERSITY
Publication Date
2019-05-10

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention successfully develops a sensor for the sensitive detection of thrombin sensitive protein-1 (TSP-1) based on the signal amplification strategy of a cerium metal organic framework @gold nanocomposite (Ce-MOF@Au) and gold-platinum-ruthenium nanocomposite (AuPtRu NP) using an aptamer as a target capture agent. The synthesized AuPtRu NP can be used not only as a catalyst for catalyzing hydrogen peroxide, but also as a nanocarrier to capture an amino group (-NH2) to terminate a single-stranded DNA (S1) to obtain a signal probe SP (AuPtRu NP / S1). Ce-MOF@Au is obtained by in-situ reduction and used as an electrode modification material of a glassy carbon electrode. When the detection solution contains an electrochemical matrix containing H<2>O<2>, AuPtRu NP can oxidize H<2>O<2> to obtain an enhanced signal. The invention shows a very low detection limit of 0.13 fg mL<-1> in the linear range from 1 fg mL<-1> to 10 ng mL<-1>. In addition, the trimetal signal amplification aptamer sensor based on cerium metal organic framework @gold nanocomposite and gold-platinum-ruthenium nanocomposite for thrombin sensitive protein-1 detection is shown to be directly usable for the detectionof clinical actual samples.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention relates to a preparation method and application of an electrochemical aptasensor for quantitatively detecting thrombin-1 in clinical practice, especially based on cerium metal-organic framework@gold nanocomposite (Ce-MOF@Au) and gold A platinum-ruthenium nanocomposite material (AuPtRu NP) is used as a biosensor prepared as a signal probe to detect thrombin-1, which belongs to the field of electrochemical detection. Background technique:

[0002] Cardiovascular disease (CVD) is a disease of the heart and blood vessels and is the leading cause of death worldwide. Individuals at risk for CVD may exhibit increased levels of biomarker molecules in the blood. Identification of biomarkers of highest CVD risk to aid in diagnosis could prevent premature death. Thrombospondin-1 (TSP-1), a promising candidate biomarker for CVD, is highly expressed in large atherosclerotic lesions and myocardial infarction. Therefore, a series of methods, such as...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More