497 results about "Streptococcus bacteria" patented technology

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which mainly utilizes a multiple fluorescent PCR analysis method to detect related pathogens, so as to simultaneously realize detection of coronavirus, bordetella pertussis, group A streptococcus, parainfluenza virus, streptococcus pneumoniae, haemophilus influenzae and group B streptococcus in a single-tube reaction system. Or detecting and distinguishing at least four of chlamydia pneumoniae. Meanwhile, the detection sensitivity of the composition is higher and reaches 500 copies / mL, and the detection is more accurate.

The invention provides a preparation method of antitoxin serum containing an horse F (ab ') 2 fragment, and the specific steps are as follows: a recombinant streptococcus immunoglobulin G degrading enzyme is immobilized to obtain an immobilized enzyme chromatography column, the recombinant streptococcus immunoglobulin G degrading enzyme comprises a glutathione-S-transferase tag and a histidine tag; performing primary treatment on the immune plasma to obtain a pretreated antibody solution; the pretreatment antibody solution is added into an immobilized enzyme chromatography column for digestion, after digestion is completed, ultrafiltration, purification and solution preparation are sequentially conducted through an immobilized glutathione affinity column or a nickel affinity column, an antitoxin serum stock solution is obtained, and the antitoxin serum stock solution contains F (ab ') 2 fragments and does not contain Fc fragments, impure protein and small molecule impurities. Through the process steps, the Fc fragment and impure protein are effectively removed, and only the horse F (ab ') 2 fragment is reserved, so that the immunogenicity of antitoxin serum and the risk of causing anaphylactic reaction are remarkably reduced.

The invention relates to a fusion protein, an immunogenic composition and a recombinant degenerate vaccine for preventing and treating infection of various pathogenic streptococci, as well as a molecular architecture design, application and the like. According to the invention, three immune antigens, namely an elongation factor Tu (Tuf), a molecular chaperone DnaK and an elongation factor G (fusA), are screened, and it is proved that fusion protein molecules of the three antigens can significantly inhibit tissue lesions caused by infection of different serotypes and different types of streptococci, have good immunogenicity, play roles in immune protection and effective prevention and treatment, and have good application prospects. The bacillus subtilis has the characteristics of broad-spectrum and high-efficiency prevention of streptococcus bacterial infection, and has a wide application prospect.

The invention discloses streptococcus thermophilus AK223-6 and application thereof in preparation of products for regulating intestinal tracts, improving memory and improving epilepsy, and belongs to the field of microorganisms and biological medicines. The invention provides streptococcus thermophilus AK223-6 which is preserved in the Guangdong Microbial Culture Collection Center on July 8, 2024, the preservation date of the streptococcus thermophilus AK223-6 is July 8, 2024, and the preservation number of the streptococcus thermophilus AK223-6 is GDMCCNO: 64835. Based on the streptococcus thermophilus, a metabiotic product is successfully researched and developed, and verification shows that the metabiotic product has the effects of regulating intestinal flora balance, promoting digestion and absorption of a human body, improving memory, improving epilepsy and reducing epilepsy times. After 100mg of a metabolite product containing 16,000,000,000 strains and metabolites is given every day, lactic acid bacteria polysaccharide, ergosterol, cephalin, vitamin A and vitamin B1 in the endophytic necessary quantity can be continuously provided in the intestinal reproduction and growth process, the memory and epilepsy can be effectively improved, the epilepsy frequency is reduced, and the practical application value is good.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention provides oral streptococcus ZRSOR-5 as well as a related product, a method and application thereof. The preservation number of the streptococcus oral cavity ZRSOR-5 provided by the invention in the Guangdong Microbial Culture Collection Center is GDMCC No: 65352, and the preservation number of the streptococcus oral cavity ZRSOR-5 provided by the invention is CGMCC No: 65352. Experiments prove that the SOR preparation is prepared by diluting the SOR preparation with normal saline and inhaling the SOR preparation into mice in advance, so that the survival rate of influenza virus infected mice can be remarkably increased, and lung inflammation and virus load can be reduced. When the ZRSOR-5 preparation provided by the invention is used in advance, pneumonia caused by influenza viruses can be resisted.

The invention discloses a preparation method and application of a cow milk antibacterial peptide with wide inhibitory activity on gram-positive bacteria and gram-negative bacteria by a fermentation method, the cow milk antibacterial peptide is prepared by taking milk protein as a raw material through a microbial fermentation method and a multi-stage membrane separation and purification process, the yield of the cow milk antibacterial peptide prepared by the method can reach 18-30%, and the yield of the cow milk antibacterial peptide prepared by the method can reach 18-30%. And the peptide content in the product is 60-85%. The cow milk antibacterial peptide prepared by the preparation method disclosed by the invention has inhibitory activity on gram-positive bacteria and gram-negative bacteria. The prepared cow milk antibacterial peptide can be used for preparing drugs and / or health care products, food and skin care products for preventing and / or reducing infectious diseases caused by escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, porphyromonas gingivalis and streptococcus mutans, can also be used as a food preservative, an oral bacteriostatic agent, a preservative and the like, and has a good application prospect.

The invention relates to streptococcus thermophilus CT-02 with an immune regulation effect and application of the streptococcus thermophilus CT-02, the classification name of the streptococcus thermophilus CT-02 with the immune regulation effect is Streptococcus thermophilus, the preservation number of the streptococcus thermophilus CT-02 is GDMCC No: 64474, and the preservation date of the streptococcus thermophilus CT-02 is March 29, 2024. The strain has an excellent immune regulation effect which is specifically shown as follows: by moderately activating macrophages, not only can the excessive activation of TNF-alpha, IL-1beta, IL-6 and IL-12 be avoided, but also the activation level of IL-10 can be improved, that is, the levels of various inflammatory factors secreted by the macrophages are comprehensively regulated, and the strain has a potential anti-inflammatory effect while activating immunity. The strain has a prospect for preparing a product with an immune regulation effect or a product with an inflammatory factor regulation effect.

The invention discloses a meningioma saliva microbial marker, a product and application of the meningioma saliva microbial marker, and belongs to the field of biological medicine. The saliva microbial marker is prepared from one or more of fusobacterium periodontium, neisseria microflavus, and Streptococcus cristatum, and the saliva microbial marker is prepared from one or more of the following components: the fusobacterium periodontium, the neisseria microflavus, and the Streptococcus cristatum, and the saliva microbial marker is prepared from one or more of the following components: the Saccharomyces cerevisiae, the Saccharomyces cerevisiae, the Saccharomyces cerevisiae and the Saccharomyces cristatum. The invention provides a kit which comprises a detection reagent for detecting the relative abundance of the saliva microbial marker. The invention provides a product for diagnosing meningioma and a computer program product related to meningioma. The product provided by the invention has good feasibility and accuracy, can effectively evaluate the risk of meningioma, and provides a new tool for clinical diagnosis.

The invention belongs to the technical field of bacterial infection reagents, and particularly relates to application of a reagent for detecting markers in tilapia in preparation of a detection product for detecting tilapia streptococcus agalactiae infection. According to the application disclosed by the invention, the expression levels of Hap and IL-6 in blood and Hep and IL-6 in spleen of the streptococcus agalactiae infected tilapia mossambica are more obviously changed compared with that of the tilapia mossambica which is not infected by the streptococcus agalactiae; whether tilapia is infected with streptococcus agalactiae or not can be accurately judged according to expression levels of Hap in tilapia blood, IL-6 in tilapia blood, Hep in tilapia spleen and IL-6 in tilapia spleen.

The invention provides application of baicalin in preparation of a medicine for treating streptococcus agalactiae induced mastitis, and relates to the technical field of medicine. A mouse is selected as an animal model to research the intervention effect and mechanism of the baicalin on the streptococcus agalactiae induced mouse mastitis inflammatory response, and it is clear that the baicalin can play a role in protecting the mouse mammary gland by regulating and controlling an NLRP3 inflammatory small body pathway; a reference basis is provided for treating the cow mastitis caused by streptococcus agalactiae infection by applying the baicalin.

The invention provides an electronic one-tube automatic detection system and a detection method for streptococcus agalactiae or gonococci, through an LAMP and CRISPR-Cas12a combined mediated one-tube visual streptococcus agalactiae or gonococci detection system, in combination with an electronic automation technology, an LAMP reaction mixture and a CRISPR-Cas12a reaction mixture are mixed to form a one-tube reaction system, and the one-tube reaction system is used for detecting the streptococcus agalactiae or gonococci. Automatic mixing, preheating reaction and fluorescence detection of various detection reagents and samples are realized, and visual display is performed on detection results by adopting visual equipment. Visual detection of bacteria is achieved by detecting fluorescence signals, rapid positive or quantity detection results can be provided for patients and other users, and clinical detection efficiency is improved.

The invention discloses application of carbazole derivatives C1-1 to C1-12, C2-1 to C2-5, C3-1 to C3-10, C4-1 to C4-3 and C5-1 to C5-2 to prevention and treatment of pathogenic bacteria of cow mastitis, and belongs to the field of natural medicinal chemistry. The compound disclosed by the invention has good inhibitory activity on the pathogenic bacteria, such as Streptococcus agalactiae ATCC27956, Streptococcus dysgalactiae ATCC27957 and Staphylococcus aureus, of the mastitis of the dairy cow, and the compound disclosed by the invention has good inhibitory activity on the pathogenic bacteria, such as Streptococcus agalactiae ATCC27956, Streptococcus dysgalactiae ATCC27957 and Staphylococcus aureus. The carbazole derivative has the characteristics of simple structure, easiness in synthesis, low price, wide application and the like, and has further research and development values in the aspect of preventing and treating the cow mastitis.

The invention discloses application of a series of quinine derivatives in preventing and treating pathogenic bacteria of cow mastitis, and belongs to the field of natural medicinal chemistry. According to the present invention, with the application of the derivative, the excellent inhibition activity on the dairy cow mastitis pathogenic bacteria such as Streptococcus agalactiae ATCC 27956 and Streptococcus dysgalactiae ATCC 27957 can be provided, and the excellent inhibition activity can be provided on the dairy cow mastitis pathogenic bacteria such as Streptococcus agalactiae ATCC 27956 and Streptococcus dysgalactiae ATCC 27957; the synthesis method of the compound is simple, and the compound has great application value in the aspect of natural product source compounds for preventing and treating cow mastitis. Structural formula of related compound as follows: # imgabs0 #

The invention relates to the technical field of biology and new medicine, in particular to a preparation method, an identification method and a histopathologic analysis method of a rat uterus scar diverticulum model. The preparation method of the rat uterus scar diverticulum model comprises the following steps: sequentially simulating rat pregnancy by using female progestational hormone, and infecting a uterus incision by using group B streptococcus (GBS) to obtain the rat uterus scar diverticulum model. The preparation method comprises the following steps: constructing a pseudopregnant rat model; preparing a GBS bacterial solution with a certain concentration, and immersing 3-0 silk threads with the length of about 1cm into the bacterial solution for 24 hours for later use; an incision with the length of about 2 cm is formed in the uterus on the two sides of the rat, and the incision is sutured discontinuously by an absorbable line. The simulated pregnant mouse is used, the influence of uterine hyperemia during human delivery and hormone change after female progestational hormone withdrawal on the uterine microenvironment is met, the formation of the human uterine scar diverticulum simulated by using the group B streptococcus infection incision is related to cesarean delivery incision infection, and therefore the success rate of the obtained model is high.

The invention provides application of polypeptide or related biological materials thereof in serving and / or preparing products for regulating oral micro-ecological balance. The polypeptide with the amino acid sequence as shown in SEQ ID NO: 1 or related biological materials thereof can selectively inhibit harmful bacteria (such as streptococcus mutans) in the oral cavity, and also can ensure normal physiological activities of probiotics (such as streptococcus sanguis, streptococcus salivarius, streptococcus gordoni and the like) in the oral cavity, so that the regulation of micro-ecological balance of the oral cavity is realized, and the oral health of a human body is ensured.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus BR32 with functions of preventing decayed teeth and improving inflammation and application of the plant lactobacillus BR32. The plant lactobacillus BR32 with the functions of preventing decayed teeth and improving inflammation, provided by the invention, has the preservation number of CGMCC No.32836, has an inhibiting effect on the growth of oral pathogens such as streptococcus mutans, candida albicans and porphyromonas gingivalis, is high in oxidation resistance, is beneficial to continuous oral cavity stability maintenance through a synergistic effect, also has relatively high hydrophobicity and self-agglutination capability, and can be used for preparing oral cavity health-care products. The compound is low in tooth environment corrosion, high in lysozyme tolerance and good in oral colonization capacity, does not have a hemolysis phenomenon in in-vitro hemolysis culture, is sensitive to common antibiotics and has relatively high food safety. The invention further provides a microbial agent, a probiotic product, a functional food, a fermented product, a bacteriostatic agent and an oral product containing the plant lactobacillus BR32 with the functions of preventing the decayed teeth and improving the inflammation, and the effects of preventing the decayed teeth, improving the inflammation, preventing dental plaque, relieving oral ulcer and the like are achieved.

The invention relates to a streptococcus thermophilus CT-01 and application of the streptococcus thermophilus CT-01 in preparation of an immunomodulatory preparation, the classification name of the streptococcus thermophilus CT-01 is Streptococcus thermophilus, the preservation number of the streptococcus thermophilus CT-01 is GDMCC No: 64473, and the preservation date of the streptococcus thermophilus CT-01 is March 29, 2024. The bacterium has an excellent immune regulation effect which is specifically shown as follows: comprehensive regulation is performed on the behavior of secreting inflammatory factors by macrophages, excessive activation of TNF-alpha, IL-1beta and IL-6 can be avoided, the level of IL-10 can be improved, and the bacterium has a potential anti-inflammatory effect while regulating immunity. The strain has a prospect for preparing a product with an immune regulation effect or a product with an inflammatory factor regulation effect.

The invention discloses application of epimedin A in preparation of a streptococcus suis type 2 capsule inhibitor, and belongs to the technical field of biological medicine. The compound can effectively inhibit the synthesis function of the streptococcus suis type 2 capsule, further enhances the removal effect of a host on the streptococcus suis type 2, reduces the pathogenicity of bacteria, and provides a new candidate compound and idea for preparing a capsule inhibitor of the streptococcus suis type 2 and researching and developing a novel medicine for treating streptococcus suis type 2 infection.

The invention discloses construction and application of a streptococcus suis serotype 2 cps2C gene knockout mutant strain. The invention relates to a streptococcus suis serotype 2 cps2C gene knockout mutant strain 05ZYH33 delta cps2C. A coding gene, from the 31st site to the 1000th site, of the cps2C gene in the strain 05ZYH33 is replaced by a spectinomycin resistance gene cassette SpcR. The mutant strain 05ZYH33 delta cps2C is applied to preparation of a streptococcus suis type 2 attenuated vaccine and a subunit vaccine. The capsule of the mutant strain 05ZYH33 delta cps2C is obviously reduced; in-vitro experiments show that the macrophage phagocytosis resistance of the mutant strain 05ZYH33 delta cps2C is reduced, and animal toxicity test results show that the toxicity of the mutant strain is obviously reduced. The mutant strain provides an important clue for screening of protective antigens of multivalent subunit vaccines, and can be applied to development of S.suis attenuated vaccines and multivalent subunit vaccines.

The invention relates to streptococcus thermophilus and application thereof, in particular to novel streptococcus thermophilus YSC1003 which has good fermentation performance and safety, and prepared fermented milk is short in fermentation time, large in viable count, high in viscosity and water-holding capacity and high in antioxidant activity. In addition, the invention also provides a compound strain containing the streptococcus thermophilus YSC1003, the compound strain has better fermentation performance and safety, and compared with a single strain, the fermentation time of the fermented milk prepared by the compound strain is obviously shortened, the viable count is obviously increased, and the fermentation time of the fermented milk prepared by the compound strain is obviously shortened under the condition of consistent bacterial quantity through strain interaction and synergistic interaction. The viscosity and the water-holding capacity are obviously improved, and the antioxidant activity is obviously improved.

The invention discloses an application of marine streptomyces SH110 and a source compound thereof, the marine streptomyces SH110 has a preservation number of GDMCC (China General Microbiological Culture Collection Center) NO.66668, and the preservation date is July 14, 2025; the invention also discloses application of the marine streptomyces SH110-derived compound in preparation of a medicine for preventing and treating streptococcus and vibrio diseases. The marine streptomyces source compound has efficient vibrio and streptococcus resisting activity, the vibrio and streptococcus resisting activity is not reported, and the marine streptomyces source compound has new application. The marine streptomyces source compound is used for preparing the medicine for preventing and treating the vibrio and streptococcus diseases, especially for preparing the medicine for preventing and treating the vibrio and streptococcus diseases of aquaculture animals, the safety is high, and the treatment effect is good.

The invention provides a probe set for detecting streptococcus pneumoniae serotype, a method and application. The probe set provided by the invention can rapidly identify streptococcus pneumoniae and classify the capsule serotype of streptococcus pneumoniae, and is suitable for serotype replacement and vaccine action monitoring.

The invention provides an application of streptococcus salivarius in fatty liver, in particular to an application of streptococcus salivarius in preparation of a product, and the product is used for at least one of the following applications: (a) losing weight or reducing body fat; (b) improving the glucose tolerance of the patient; (c) increasing the sensitivity of the patient to insulin; (d) treating, relieving and preventing liver injury; (e) treating, relieving and preventing non-alcoholic steatohepatitis; (f) fat deposition caused by high-fat and high-cholesterol diet is reduced; (g) reducing blood fat; and / or (h) improving the level of at least one of the following indexes in the liver of the mammal: total cholesterol, triglyceride, low density lipoprotein-cholesterol and free fatty acid.

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which is mainly used for detecting related pathogens by using a multiple fluorescent PCR (Polymerase Chain Reaction) analysis method. Therefore, at least four of the klebsiella aerogenes, the stenotrophomonas maltophilia, the streptococcus angina, the acinetobacter baumannii, the klebsiella pneumoniae and the klebsiella oxytoca can be simultaneously detected and distinguished in a single-tube reaction system. Meanwhile, the detection sensitivity of the composition is higher and reaches 500 copies / mL, and the detection is more accurate.

The invention provides a composition and application thereof in caries prevention. The composition comprises fluoride and polypeptide, the concentration of F <-> in the fluoride in the composition is 1.79 to 7.14 mM; the concentration of the polypeptide in the composition is 1.56-12.5 [mu] M, and the amino acid sequence of the polypeptide is as shown in SEQ ID NO: 1. The natural advantage that polypeptide is not prone to drug resistance is utilized, the risk that streptococcus mutans and other bacteria generate drug resistance is greatly reduced, the inhibition effect of fluoride on streptococcus mutans can be remarkably enhanced, and a good anti-caries effect can be achieved under the low concentration (F <-> is 1.79-7.14 mM) of fluoride. Moreover, adverse effects on probiotics in the oral cavity are avoided, and the oral micro-ecological balance can be adjusted. Therefore, the composition disclosed by the invention is suitable for preparing products for preventing caries, inhibiting streptococcus mutans and regulating oral micro-ecological balance.

Compositions comprising 5. salivarius strain SALI-10 and / or salivarius 10 peptide are taught. Also taught are methods of treating bacterial infections comprising administering a pharmaceutically acceptable amount of the composition to the patient with a bacterial infection caused by a pathogen from, for example, multi-drug resistant (MDR) Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus dysagalactiae, vancomycin-resistant Enterococcus faecium, Porphyromonas gingivalis, Tannerella forsythia, and MDR Enterococcus faecalis.

The invention relates to an Alzheimer's disease marker based on intestinal flora and a risk prediction model. The Alzheimer's disease marker based on the intestinal flora comprises the intestinal flora, wherein the intestinal flora comprises anaerobic lawsonia pasteurianus, escherichia coli, streptococcus salivarius, assa wig, clostridium luviergii, intestinal rosebawii, rosella faecalis, human rosella, trichothecium CAG424 and clostridium CAG58. The invention further discloses a preparation method of the alzheimer's disease marker based on the intestinal flora, and the alzheimer's disease marker based on the intestinal flora comprises the intestinal flora and an application of the alzheimer's disease marker based on the intestinal flora. The Alzheimer's disease marker based on the intestinal flora is excavated, the Alzheimer's disease risk prediction model based on the intestinal flora is further constructed, and the method has the advantages of being high in detection accuracy, convenient, rapid, safe and noninvasive and has important clinical guiding significance in auxiliary diagnosis of Alzheimer's disease related indexes.

The invention provides a streptococcus suis polysaccharide protein conjugate vaccine as well as a preparation method and application thereof, and belongs to the technical field of vaccine preparation. The vaccine comprises a polysaccharide-protein conjugate formed by coupling streptococcus suis type 2 capsular polysaccharide and streptococcus suis type 2 recombinant protein. The preparation method comprises the following steps: S1, preparing streptococcus suis type 2 capsular polysaccharide; s2, preparing streptococcus suis type 2 recombinant protein; s3, activating polysaccharide; s4, combining the biotinylated polysaccharide with the recombinant protein containing the avidin structural domain to form a stable polysaccharide-protein conjugate; s5, removing unbound proteins; and S6, mixing the polysaccharide-protein conjugate with the oil-water emulsion in proportion. By optimizing a coupling technology and a vaccine preparation formula, the immunogenicity and the protection effect of the vaccine are improved, the safety and the broad-spectrum cross protection capability of the vaccine are remarkably enhanced, the complexity and the cost of a vaccine production process are reduced, and the vaccine has a good industrial application prospect and a good popularization value.

The invention provides a polypeptide and application thereof in preparation of a streptococcus mutans inhibitor. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, and the polypeptide not only can effectively inhibit streptococcus mutans, but also is non-toxic to cells, and is suitable for preparing oral products which are excellent in antibacterial effect and can be used for a long time, so that oral diseases, especially caries, are prevented and treated. Moreover, the polypeptide disclosed by the invention is short in length, small in chemical synthesis difficulty, capable of being directly synthesized into a high-purity product, clear in effect and wide in application.