Sterilization formulation and wet tissue, preparation method and use

A technology of fungicides and wet wipes, applied in the direction of botany equipment and methods, applications, biocides, etc., can solve the problems of high production cost, difficulty in popularization and application, and ineffective effect of Staphylococcus aureus, so as to reduce the possibility of infection Sexuality, guaranteed cleaning effect

Inactive Publication Date: 2005-07-13
昆山博青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The wet wipes can remove various pathogenic bacteria that cause mammary gland infection, but the effect on drug-resistant Staphylococcus aureus is not obvious. At the same time, due to the complex synthesis process of antimicrobial peptides and high production costs, it is difficult to popularize and apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of antiseptic wipes

[0030] 1. prepare 100g for the solution of bactericidal wet tissue, wherein lysostaphin content 0.001g (0.001wt%), lysozyme content 20g (20wt%), polyethylene glycol content 3g (3%), polysorbate Ester 20 content 0.2g (0.2wt%).

[0031] 2. Disinfect tabletops, instruments, utensils, and ingredient barrels.

[0032] 3. Accurately weigh lysostaphin, lysozyme, polyethylene glycol and polysorbate 20 on an electronic balance, place them in the batching bucket, add 76.80g of water in proportion, stir evenly, and heat to 60°C to dissolve, That is the desired solution.

[0033] 4. Cut the non-woven fabric into 8cm×8cm, sterilize it with ultraviolet light for 20 minutes, immerse it in the above solution, remove it after soaking, drip off the excess solution, and then use it.

Embodiment 2

[0035] In Vitro Bactericidal Test of Bactericidal Wet Wipes

[0036] 1. Preparation of test samples:

[0037] (1) Sterilizing wet tissue sample: prepared according to the method of Example 1, wherein the content of lysostaphin is 0.001wt%, the content of lysozyme is 20wt%, the content of polyethylene glycol is 3%, and the content of polysorbate 20 is 0.2wt%. .

[0038] (2) Contrast wet tissue: wherein the content of polyethylene glycol is 3%, and the content of polysorbate 20 is 0.2 wt%.

[0039] 2. Test strains: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli and Corynebacterium pyogenes (the above strains are from the Military Horse Control Institute of Shenyang Military Region).

[0040] 3. Test steps:

[0041] (1) Wash the fresh slope of the cultivated test strain with 1% peptone PBS, and dilute it to an appropriate concentration. 7 ~10 8 cfu / ml.

[0042] (2) The sample wet tissue and the control wet...

Embodiment 3

[0058] In Vitro Bactericidal Test of Bactericidal Wet Wipes

[0059] 1. Preparation of test samples:

[0060] (1) Sterilizing wet tissue sample: prepared according to the method of Example 1, wherein the content of lysostaphin was 0.001wt%, the content of lysozyme was 20wt%, the content of polyethylene glycol was 3%, and the content of betaine was 0.2wt%.

[0061] (2) Contrast wet tissue: wherein the content of polyethylene glycol is 3%, and the content of betaine is 0.2 wt%.

[0062] 2. Test strains: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli and Corynebacterium pyogenes (the above strains are from the Military Horse Control Institute of Shenyang Military Region).

[0063] 3. Test procedure: Carry out according to embodiment 2.

[0064] 4. Test results

[0065] Table 2: Killing rate results of germicidal wet wipes in vitro sterilization test

[0066] Test strain Antiseptic wipes Contro...

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Abstract

The invention discloses a bactericidal preparation, which contains 0.0001-0.1 wt% of lysostaphin, 10-50 wt% of lysozyme, 1-15 wt% of a stabilizer, 0.1-0.5 wt% of a surfactant and the balance of water. The invention also discloses a bactericidal wet tissue, its preparation method and its application in treating and preventing milk cow mammary gland infection. The bactericidal wet tissue can be obtained by loading the bactericidal preparation on a carrier. The bactericidal wet tissue provided by the invention is used to kill various bacteria that cause milk cow mammary gland infection, and can achieve very significant effects, especially for E. , Corynebacterium pyogenes killing rate of more than 99%.

Description

technical field [0001] The invention relates to a bactericidal preparation and a bactericidal material, in particular to a bactericidal preparation and a bactericidal wet tissue for killing bacteria that cause milk cow mammary gland infection. Background technique [0002] Dairy cow mammary gland infection is mainly caused by Staphylococcus aureus, Escherichia coli, Streptococcus, Pseudomonas aeruginosa, etc. Herd surveys show that 30% to 70% of cows in the United States have at least one infected udder area. Mastitis caused by mammary gland infection in dairy cows causes a loss of 0.5 kg of milk production per day, and the reduced milk production is estimated to be 69%-80% of the total loss due to mastitis. The U.S. dairy industry loses more than $2 billion annually in direct losses from mastitis. It can be seen that the economic impact of mastitis on the dairy industry is huge. [0003] At home and abroad, large doses of antibiotics (such as penicillin, streptomycin, gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A61L2/16A61L15/46
Inventor 黄青山陆婉英
Owner 昆山博青生物科技有限公司
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