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Streptococcus and dairy cow mastitis vaccine obtained by inactivating streptococcus

A technology for dairy cow mastitis and Streptococcus lactis, which is applied in bacterial antigen components, sexually transmitted diseases, drug combinations, etc., can solve the problems of high actual loss, high incidence of dairy cows, spread of pathogenic microorganisms, etc., and achieves the effect of various dosage forms.

Active Publication Date: 2014-05-21
INNER MONGOLIA HUAXI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The recessive type has no specific clinical manifestations and can only be detected through somatic cells, milk production and quality testing, but the attack rate of dairy cows is much higher than that of the clinical type, causing further spread of pathogenic microorganisms, and under certain conditions it will transform into clinical symptoms. type, the actual loss is much higher than the clinical type

Method used

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  • Streptococcus and dairy cow mastitis vaccine obtained by inactivating streptococcus
  • Streptococcus and dairy cow mastitis vaccine obtained by inactivating streptococcus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The acquisition and preservation of embodiment 1, bacterial strain

[0035] 1. Screening of strains

[0036] 30,000 cow milk samples were collected from 90 medium and large dairy farms in 30 provinces and cities including Inner Mongolia, Chongqing, Guangzhou, Heilongjiang, Lanzhou, Hebei, and Shandong. Milk sample collection method of dairy cows: select cows with clinical symptoms of mastitis (or suspected subclinical mastitis), first scrub the udders with warm water, then scrub the udders with 0.2% bromogeramine, and finally wipe the nipples with 70% alcohol. At the same time, use 70% alcohol to wipe and disinfect the fingers. Squeeze out the first 2-3 handfuls of milk from each milk chamber to eliminate contaminated bacteria. Take at least 5mL of milk samples from each cow in a sterile milk sample cup.

[0037] Isolation and purification of Streptococcus strains from milk samples. The colony characteristics of Streptococcus (37°C, cultured for 24-72h): (1) no coloni...

Embodiment 2

[0051] Embodiment 2, the characteristic of bacterial strain

[0052] The cryopreservation tube obtained in Step 2 of Example 1 and 3 was thawed at room temperature, and the P3 generation strain was subjected to the following steps:

[0053] 1. Immunogenicity of strains

[0054] Rabbits were immunized with the P3 generation strain SAWR-6 (single immunization, each by subcutaneous injection of 5×10 7CFU / ml, 1 ml for each injection), 21 days later, blood was collected from the heart, and the serum was separated. The IgG antibody titer of the serum was detected by ELISA (serum dilution was obtained by gradient dilution with PBST buffer; the P3 generation strain SAWR-6 was used as the coating source, and the coating concentration was 1×10 9 Bacteria / ml; goat anti-rabbit IgG enzyme-labeled secondary antibody; set negative control, that is, replace serum diluent with PBST buffer; detect the absorbance value at 450nm; the absorbance value is more than 2.1 times that of the negative ...

Embodiment 3

[0072] Embodiment 3, the preparation of vaccine

[0073] 1. Preparation of capsular polysaccharide

[0074] The cryopreservation tube obtained in Step 2 of Example 1 and 3 was thawed at room temperature, and the P3 generation strain was subjected to the following steps:

[0075] 1. Take 3-5 typical colonies of the strain SAWR-6, inoculate them in BHI medium, and cultivate them with shaking at 37°C and 150rpm for 18h.

[0076] 2. Take 2 parts by volume of the bacterial liquid obtained in step 1, inoculate 100 parts by volume of BHI medium, and ferment in a fermenter at 37°C (at the initial stage of fermentation, the bacterial concentration of strain SACP5-9 is 2×10 8 per ml, the temperature of the fermenter is 37°C, the initial rotation speed of the fermenter is 198r / min, the tank pressure is maintained at 0.05MPa, and the pH of the fermentation system is adjusted to 7.0-7.2 with 8% NaOH aqueous solution or 5% hydrochloric acid aqueous solution. Rotate the speed of the fermen...

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Abstract

The invention discloses streptococcus and a dairy cow mastitis vaccine obtained by inactivating the streptococcus. The active components of the dairy cow mastitis vaccine are an inactivated strain SAWR-6 (streptococcus agalactiae-6), an inactivated strain SDTR-9 (streptococcus dysgalactiae-9) and an inactivated strain SURF-5 (streptococcus uberis-5). The invention also provides a dairy cow mastitis vaccine, of which the active components are inactivated strains and capsular polysaccharides, wherein the inactivated strains are an inactivated strain SAWR-6, an inactivated strain SDTR-9 and an inactivated strain SURF-5; the capsular polysaccharides are strain SAWR-6 capsular polysaccharide, strain SDTR-9 capsular polysaccharide and strain SURF-5 capsular polysaccharide. The vaccine disclosed by the invention has the advantages of high efficiency, universality, safety, stability, various preparations and the like, a powerful guarantee is provided to safe, effective and comprehensive and effective prevention of streptococcus infected dairy cow mastitis, and the vaccine is a new milestone for the development of dairy cow mastitis vaccine.

Description

technical field [0001] The invention relates to streptococcus and a cow mastitis vaccine obtained by inactivating the streptococcus. Background technique [0002] Bovine Mastitis is a major infectious disease that endangers the development of animal husbandry and food safety. Especially in recent years, with the change of the environment and the abuse of antibiotics, a variety of drug-resistant bacteria and even superbugs have emerged. The morbidity and mortality of dairy cow mastitis caused by infection are increasing year by year, seriously endangering the development of animal husbandry and food safety, and further endangering human health. The economic losses caused by cow mastitis amount to 3 billion U.S. dollars in the United States, 400 million U.S. dollars in the United Kingdom, and more than 3.5 billion U.S. dollars in my country. Cow mastitis is divided into clinical type and recessive type. The symptoms of the clinical type are: fever, swelling and pain in the b...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61P15/14A61P37/04
Inventor 孙艺萍王林叶孙治华王欢李敏段跃强杨鹏辉尚炜
Owner INNER MONGOLIA HUAXI BIOTECH
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