The invention discloses an extraction method for persimmon
tannin. The method comprises the following steps: firstly, persimmons are peeled and crushed,
ethanol is added, extraction is carried out, the extracting solution is filtered, and the filtrate is taken for standby; secondly, the filtrate is condensed, 95V% of
ethanol is added, the mixture is allowed to stand overnight,
centrifugation is carried out, and a supernatant is taken; thirdly, the supernatant is sieved through AB-8
macroporous resin, a column is washed by utilization of water until the column is colorless, thenthe column is washed by utilization of
ethanol, and the ethanol eluate is collected; fourthly, the ethanol eluate is condensed, the
solvent is recovered, an extract is obtained, the extract is dissolved in water, filtering is carried out, the filtrate is taken and is sieved through a hollow
fiber film with a
cut-off molecular weight of 450-600, and the
cut-off solution is collected for standby; fifthly, the
cut-off solution is sieved through a hollow
fiber film with a cut-off molecular weight of 14000-15000, the penetration solution is collected and dried, and persimmon
tannin power is prepared. The extracted persimmon
tannin has a good function of inhibiting
streptococcus mutans, and can be used for caries prevention.