39 results about "Mutans streptococcus" patented technology

The present invention provides a safe and highly effective antibacterial composition without impairing the flavor of foods or drinks, which composition is used in products such as foods and oral care goods which are mainly to be taken into human or animal bodies or mainly to be used in the oral cavity. The composition according to the present invention contains gallocatechin gallate as an agent for enhancing the activity of antibacterial catechins in which the antibacterial activity of the antibacterial catechins against Streptococcus mutans is particularly enhanced. The composition according to the present invention can be produced by mixing the antibacterial catechins with gallocatechin gallate. Alternatively, it can be produced as a composition comprising a synthetic adsorbent-adsorbed fraction as the main component, which is obtained by subjecting a solvent-extract from tea leaves to an adsorption treatment with the use of a synthetic adsorbent selected from among aromatic compound-based synthetic adsorbents and methacrylic compound-based synthetic adsorbents.

The present invention provides glucan-based compositions and methods for stimulating an immune response against mutans streptococci components and vaccines and methods for the treatment and prevention of dental caries. In a preferred embodiment, a glucan polymer, preferably WSG, is covalently bound to one or more T cell-dependent antigens to form a conjugate vaccine. The T cell-dependent antigen preferably contains epitopes of one or more mutans streptococcal proteins, such as a glucosyltransferase. Moreover, one or more moieties, including haptens, may be conjugated to the glucan or to the glucan-T cell-dependent composition. In a preferred embodiment, these moieties are peptides which contain immunogenic epitopes corresponding to components of a mutans streptococcus.

The invention provides a decayed-tooth-resistant chewing gum, which comprises a gum base and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 8-15 parts of siraitia grosvenorii, 1-5 parts of radix glycyrrhizae, 1-3 parts of gallnut, 1-2 parts of radix angelicae dahuricae, 0.5-1.5 parts of mint, 0.5-1 part of clove, 1-3 parts of lotus leaf and 1-3 parts of cortex magnoliae officinalis. According to the invention, the combining capacity of active ingredients in the decayed-tooth-resistant chewing gum and streptococcus mutans is higher, and the active ingredients can directly enter the streptococcus mutans cells to play the effect of killing bacteria.

The invention relates to a preparation method and process for antibacterial modification of ceramic whiskers and in particular provides a preparation method for fusion-coating of zinc oxide nanoparticles having different patterns on the surface of hydroxyapatite whiskers. The preparation method is characterized by adding dropwise an ethanol solution of a zinc salt into a beaker in which an ethanol solution of hydroxyapatite whiskers is held, performing stirring, rotating, ultrasonic treatment, drying and calcination procedures to make zinc oxide-hydroxyapatite whiskers. The ceramic whiskers have antibacterial effects. The zinc oxide nanoparticles have good antibacterial effects and the antibacterial rate against Streptococcus mutans reaches (93.58 plus or minus 5.95) percent; and the hydroxyapatite whiskers have high strength. After modification, zinc oxide nanoparticles, nanospheres, nanorods, microspheres and diamond-shape units are uniformly distributed on the surface of the hydroxyapatite whiskers by fusion coating, the particle size is 30 nm to 400 nm, the surface area and the roughness are greatly improved, and the mechanical embedment force between the zinc oxide nanoparticle and the matrix is increased. Therefore, the zinc oxide-modified hydroxyapatite whisker can serve as an ideal reinforcing material with antibacterial effect.

The present invention provides glucan-based compositions and methods for stimulating an immune response against mutans streptococci components and vaccines and methods for the treatment and prevention of dental caries. In a preferred embodiment, a glucan polymer, preferably WSG, is covalently bound to one or more T cell-dependent antigens to form a conjugate vaccine. The T cell-dependent antigen preferably contains epitopes of one or more mutans streptococcal proteins, such as a glucosyltransferase. Moreover, one or more moieties, including haptens, may be conjugated to the glucan-T cell-dependent composition. In a preferred embodiment, these moieties are peptides which contain immunogenic epitopes corresponding to components of a mutans streptococcus.

The invention discloses an extraction method for persimmon tannin. The method comprises the following steps: firstly, persimmons are peeled and crushed, ethanol is added, extraction is carried out, the extracting solution is filtered, and the filtrate is taken for standby; secondly, the filtrate is condensed, 95V% of ethanol is added, the mixture is allowed to stand overnight, centrifugation is carried out, and a supernatant is taken; thirdly, the supernatant is sieved through AB-8 macroporous resin, a column is washed by utilization of water until the column is colorless, thenthe column is washed by utilization of ethanol, and the ethanol eluate is collected; fourthly, the ethanol eluate is condensed, the solvent is recovered, an extract is obtained, the extract is dissolved in water, filtering is carried out, the filtrate is taken and is sieved through a hollow fiber film with a cut-off molecular weight of 450-600, and the cut-off solution is collected for standby; fifthly, the cut-off solution is sieved through a hollow fiber film with a cut-off molecular weight of 14000-15000, the penetration solution is collected and dried, and persimmon tannin power is prepared. The extracted persimmon tannin has a good function of inhibiting streptococcus mutans, and can be used for caries prevention.

The present invention provides Lactobacillus sialicus and application thereof in down-regulating gene expression of streptococcus mutans biofilm formation, belonging to the field of microbial technology. Lactobacillus salivarius ZM06 according to the present invention is deposited under the accession number CGMCC No. 13978. As the lactobacillus sialicus ZM06 of the invention can normally grow in alysozyme solution with a concentration of 0.2 mg / mL to 1 mg / mL, the oral high lysozyme concentration can be completely tolerate. The lactobacillus sialicus ZM06 of the invention can grow normally ina lysozyme solution with a concentration of 0.2 mg / mL to 1 mg / mL, and thus can tolerate oral high lysozyme concentration. As that lactobacillus sialicus has good tolerance to lysozyme, the lactobacillus sialicus is adapt to oral environment, does not aim to kill streptococcus mutans, but specifically inhibit the formation of streptococcus mutans biofilm by reduce the expression level of genes related to biofilm formation in streptococcus mutans, so as to reduce the occurrence and development risk of dental caries.

The invention provides the tools needed for a dental patient's caries risk assessment. Included is a complete turnkey system for caries risk assessment and treatment, including an ATP test and a plurality of rinses and educational and diagnostic materials. The system for the detection and treatment of the bacterial infection that causes dental caries in a patient comprises a disposable ATP Bioluminescence sampler to swab areas of the patient's mouth, a bioluminescent light meter which measures the amount of ATP present in the patient's mouth, a rapid culture test for the detection of Mutans streptococci and lactobacilli, a diagnostic testing component that includes a caries risk assessment questionnaire, a treatment component that includes a short term therapeutic oral rinse of at least two components, and a long term maintenance rinse.

The invention discloses a method for extracting tannin from persimmons. The method includes the following steps that 1, the persimmons are peeled and smashed, ethyl alcohol is added for extraction, extract is filtered, and filtrate is taken for use; 2, the filtrate is concentrated, 95v% ethyl alcohol is added, the mixture is placed over night and then centrifuged, and supernate is taken; 3, the supernate is made to pass through AB-8 macroporous resin, treated by a washing pillar to become colorless and then eluted through ethyl alcohol, and ethyl alcohol eluant is collected; 4, the ethyl alcohol eluant is concentrated, solvent is recovered, extractum is obtained, the extractum is added with water to be dissolved and then filtered, filtrate is taken and made to pass through a hollow fiber film with the molecular weight cut-off of 400-500, and cut-off liquid is collected for use; 5, the cut-off liquid is made to pass through a hollow fiber film with the molecular weight cut-off of 13000-15000, permeating liquid is collected and dried, and then the persimmon tannin powder is obtained. The extracted persimmon tannin has an excellent inhibiting effect on streptococcus mutans and can be used for preventing caries.

The invention provides a tooth-protecting buccal tablet, belonging to the field of tooth-protecting health care. The buccal tablet can effectively suppress adhesion and acid production of streptococcus mutans, thus achieving better effects on preventing decayed teeth and improving mouth health. The buccal tablet comprises a food adhesive, a filler and fructus momordicae extracts, wherein the fructus momordicae extracts account for 1-30wt% of total buccal tablet; and glycosides in the fructus momordicae extracts account for 5-95wt% of total fructus momordicae extracts. The buccal tablet is used for preventing decayed teeth and improving mouth health.

The invention discloses a method for extracting high-molecular weight tannin from persimmons. The method includes the following steps that 1, the persimmons are peeled and smashed, ethyl alcohol is added for extraction, extract is filtered, and filtrate is taken for use; 2, the filtrate is concentrated, 95V% ethyl alcohol is added, the mixture is placed over night and then centrifuged, and supernate is taken; 3, the supernate is made to pass through AB-8 macroporous resin, treated by a washing pillar to become colorless and then eluted through ethyl alcohol, and ethyl alcohol eluant is collected; 4, the ethyl alcohol eluant is concentrated, solvent is recovered, extractum is obtained, the extractum is added with water to be dissolved and then filtered, filtrate is taken and made to pass through a hollow fiber film with the molecular weight cut-off of 4000-5000, and cut-off liquid is collected for use; 5, the cut-off liquid is made to pass through a hollow fiber film with the molecular weight cut-off of 10000-12000, permeating liquid is collected and dried, and then the high-molecular weight tannin powder is obtained. The extracted high-molecular weight tannin has an excellent inhibiting effect on streptococcus mutans and can be used for preventing caries.

A pretreatment kit and a pretreatment kit for saliva in identification and quantitative determination of mutans streptococci by immunochromatography utilizing an antigen-antibody reaction, which can remove aggregation caused by mucin and chain formation of mutans streptococci in saliva in a simple operation and can efficiently flow out a complex of a labeled antibody and mutans streptococci from a porous membrane retaining the labeled antibody, contains (A) a 0.01 to 10 mol / L aqueous solution of sodium hydroxide, (B) a 0.01 to 3 mol / L aqueous solution of tartaric acid and / or citric acid, and (C) a nonionic surface active agent and / or an amphoteric surface active agent, in which the component (C) is mixed with the components (A) and / or (B), or is provided separately, and at least one substance selected from the particular metallic salts is contained in at least one of the components (A), (B) and (C) in an amount of 5 to 25% by weight.

The present invention provides nisin oral refreshing and tasting chips and a preparation method therefor, belonging to the technical field of leisure foods. The oral refreshing and tasting chips are prepared from the following raw materials: food-grade pulullan, an auxiliary member agent, a high sweetening agent, an emulsifier, nisin, menthol, caramel pigment and citric acid. The preparation method comprises: firstly, food-grade pulullan and the auxiliary member agent are slowly added into water at 60-70 DEG C to get dissolved and swelled, and the solution is stirred at the same time; the PH is adjusted with a moderate amount of citric acid, afterwards, the solution is allowed to stand to obtain a glue solution; nisin, the edible pigment and menthol are added into glue solution which is stirred evenly prior to standing; the emulsifier is added with stirring, the solution is cooled down to room temperature, so that bubbles in a membrane solution are removed; and an organic glass sheet is coated with the membrane solution, then the member gets dry and gets stripped, thereby obtaining the oral refreshing and tasting chips. The oral refreshing and tasting chips provided by the invention can effectively restrain the growth of streptococcus mutans and lactobacillus in the process of occurrence of dental caries; the product can effectively prevent dental caries caused by bacteria breeding, and without long-term chewing, also eliminate bad breath and keep oral cavity fresh and sanitary.

The invention relates to a chewing gum containing herba ecliptae and a preparation method thereof. The chewing gum is prepared from herba ecliptae extract, clove, herba menthae extract, a gum base, a sweetener and essence. The preparation method comprises the following steps: crushing all the components; uniformly mixing; pouring and molding; cooling. The pharmaceutical effective component herba ecliptae extract in the chewing gum has a remarkable synergistic effect when being combined with the clove and the herba menthae extract; an in-vitro antibacterial experiment result shows that the chewing gum has remarkable inhibition effects on main bacteria including streptococcus mutans and actinomyces viscosus which cause decayed teeth in oral cavities, so that the chewing gum can be used for preventing and treating oral cavity diseases caused by the bacteria, such as the decayed teeth, pulpitis, periapical periodontitis, gingivitis and the like.

The invention discloses multifunctional toothpaste containing hetian jade mineral elements. The toothpaste is prepared from the following components: a humectant, an abrasive, a thickener, a surfactant, essence, water, saccharin, polyethylene glycol, sodium dihydrogen phosphate, nano-scale hetian jade powder, quercetin and sodium benzoate. The multifunctional toothpaste disclosed by the inventioncan remove plaque bacteria, prevent generation of teeth stains and inhibit growth and multiplication of porphyromonas gingivalis and streptococcus mutans in an oral cavity, so as to achieve a tooth decay prevention effect; furthermore, by cooperation of the nano-scale hetian jade powder and the quercetin in the toothpaste, healing of dental ulcers can be promoted.

The invention relates to the technical field of metal smelting, in particular to an aluminum ash treatment method, which can significantly reduce the content of fluorine element in recycled aluminum material and final waste, and increase the aluminum content in the recycled product. The main process steps include S1: use crushed water hyacinth powder as a carbon source to cultivate Streptococcus mutans; S2: pour aluminum ash into a culture tank containing a large amount of Streptococcus mutans, stir evenly, and use Streptococcus mutans to Ash processing; S3: Take out the processed aluminum ash, drain it, and put it into the rotary furnace for ash frying; S4: Pour out the aluminum water in the rotary furnace, and add Mimi Aluminum to it, and stir evenly ;S5: Pour out the aluminum water again, and cool the aluminum water to obtain slag ingots; S6: Transfer the remaining ash in the rotary furnace to the cold ash bucket for cooling; S7: Turn on the ball mill in the cold ash bucket; S8: Turn on the magnetic separator, Collect the materials in the cold ash bucket according to fine ash, aluminum and large aluminum slag.

The invention provides a purification method of a candidate antigen Glu of streptococcus mutans recombinant subunit genetic engineering vaccine. The purification method comprises the steps as follows: fermented escherichia coli thalli of Glu protein of the streptococcus mutans recombinant subunit genetic engineering vaccine are collected, high-pressure homogenizing and thallus breaking are performed, a supernatant is collected and centrifuged, and precipitation is collected; inclusion body washing is performed; inclusion body pyrolysis is performed; a PBS (phosphate buffer solution) containing urea is adopted for resuspension and centrifugation, precipitation is removed, and a supernatant is stored; preliminary renaturation: the PB solution containing urea is taken, an inclusion body dissolution solution is dropwise added into the mixed buffer solution slowly for centrifugation, precipitation is removed, and a supernatant is subjected to column purification and affinity chromatography purification: target protein is purified under the condition of the PB solution containing urea, a PB solution containing sodium fluoride is adopted to perform enzyme digestion elution after the target protein is combined with an additive, and a collected eluent is the Glu protein subjected to chromatographic purification. The antigen purified with the method is high in purity, and the technology is simple.

Stannous fluoride polyol solid solutions combine three complimentary, biochemical mechanisms attributed to Sn++, F− and Polyol, respectively to reduce growth and metabolism of Streptococcus mutans while effecting superior Bioactivity Quotients. The stannous fluoride polyol solid solution particulate compositions of the present invention comprise:(a) stannous fluoride at between about 0.01 and about 0.8% by weight;(b) a polyol at between about 0.1 and about 30% by weight;(c) an astringency neutralizer at between about 0.01 and about 0.4% by weight, where the ratio of astringency neutralizer to stannous fluoride is from between about 0.01 and about 0.2;(d) a mucoadhesive at between about 1.5 and about 70% by weight, wherein the ratio of mucoadhesive to stannous fluoride polyol is from between about 7 to 1 and about 25 to 1;(e) a pH stabilizer, selected from the group consisting of malic, fumaric, citric acid and combinations thereof, wherein the ratio of pH stabilizer to stannous fluoride polyol is from between about 0.03 and 5 and preferably from between about 0.1 and about 3; and(f) optional flavorants, stabilizers, preservatives, conditioners, and oral care adjuncts.

The invention discloses a preparation method and application of a magnolol-carboxyl terminal polyamide controlled release formulation. The preparation method comprises the following steps: mixing dimethyl sulfoxide and double distilled water according to a volume ratio of 1:7 so as to obtain a mixed solution; taking the mixed solution, adding carboxyl terminal polyamide according to a ratio of 6mg / mL, and adding magnolol according to a ratio of 2.0mg / mL; performing ultrasonic oscillation, and fully stirring; adding water and centrifuging to remove the precipitate, and freeze-drying the supernatant, thereby obtaining the needed magnolol-carboxyl terminal polyamide controlled release formulation, namely M-PAMAM-COOH. The preparation method disclosed by the invention is simple in operation and high in feasibility; the prepared M-PAMAM-COOH overcomes the application defects of the magnolol, and the high polymer material PAMAM-COOH is combined with a drug, so that an excellent effect of thedrug can be achieved, the drug release time is prolonged, clinical application is facilitated, and the prepared M-PAMAM-COOH has an effect of inhibiting streptococcus mutans and bio-membranes and canbe used for prevention and treatment of dental caries.

The invention relates to a xylitol biscuit. The biscuit comprises, by weight, 36-40% of flour, 10-12% of rice sheets, 4-6% of oil powder, 16-18% of malt flavors, 8-10% of whole milk powder, 14-16% of xylitol, 4-6% of fresh milk, 0.12% of composite vitamin and 0.06% of vanilline. By the aid of the technical scheme, the xylitol can inhibit the growth of streptococcus mutans and reduce the attaching capability of the streptococcus mutans, the bacteriostatic action is achieved, the caries cannot be caused easily, the good caries resistant effect is achieved, the cost is low and the biscuit is suitable to mass consumption.

The invention discloses a method for evaluating the formation amount of a streptococcus mutans biological membrane. The method comprises the following steps that 11, stainless steel beads are immersedin saliva for incubation; 12, the stainless steel beads are taken down after being sucked and washed, and are placed in culture holes of a 24-hole plate; 13, a BHI liquid culture medium, a streptococcus mutans strain suspension and a to-be-detected sample solution are added, and incubation is carried out again; 14, the stainless steel beads are sucked, washed, taken down and put into the cultureholes of the 24-hole plate to be naturally dried; 21, a crystal violet solution is added for dyeing, the stainless steel beads are sucked, washed, taken down and put into the culture holes of the 24-hole plate to be naturally dried; 22, absolute ethyl alcohol is added for decolorizing, then the stainless steel beads are transferred to a 96-hole plate, and OD is measured to be 600 nm through a microplate reader. The invention also discloses application of the method in screening a streptococcus mutans biological membrane inhibitor. Compared with a traditional method, the method is easy to operate, low in cost, high in data stability and sensitivity and good in application prospect.

The present invention discloses a composition for restraining growth of oral pathogenic bacteria. The composition contains an effective dose of bacillus subtilis (berlese probiotics BS 139), a diluents, an excipient or a carrier. The growth of the oral pathogenic bacteria is restrained through antibiotics in short-chain polypeptides secreted by the bacillus subtilis. The oral pathogenic bacteria are streptococcus mutans, and the bacillus subtilis are bacterial strains which are preserved in the NRRL of Agricultural Research Service and whose preservation No. is NRRL No. B-50347. Additionally, the bacillus subtilis can also be used as a method for restraining the growth of the oral pathogenic bacteria, where the abovementioned composition is applied to individuals.

The present invention provides an ethanol-free liquid oral composition, which has an excellent plaque adhesion inhibitory effect and excellent bactericidal power against Streptococcus mutans in tartar, and has improved appearance stability and palatability. good. A liquid oral composition comprising (A) a cationic bactericide and (B) hydroxyethylcellulose dimethyl diallyl chloride in a substantially ethanol-free liquid oral composition At the same time as ammonium chloride, and use (C) anethole, wherein the mass ratio of (C) component / [(A) component + (B) component] is in the range of 0.1 to 10, and mixing (D) polyhydric alcohol and / or (E) nonionic surfactant. A method for stably mixing (A) and (B) components in the composition, characterized in that, while mixing (A) and (B) components in the liquid oral composition, (C) Components, wherein (C) / [(A)+(B)] is in the range of 0.1 to 10, and (D) and / or (E) components are mixed.

The invention discloses throat-clearing and sore-throat-relieving candies. The throat-clearing and sore-throat-relieving candies are prepared from the following ingredients: isomaltitol, boat-fruited sterculia seeds, semen oroxyli, liquorice roots, radix platycodonis, radish seeds, Chinese olive fruits, Chinese atractylodes, and water. The throat-clearing and sore-throat-relieving candies disclosed by the invention contain the following ingredients: the extracts of boat-fruited sterculia seeds, semen oroxyli, liquorice roots, radix platycodonis, radish seeds, Chinese olive fruits and Chinese atractylodes; thus, the throat-clearing and sore-throat-relieving candies have the functions of clearing away heat, detoxifying, clear the throat, relieving sore throat, benefiting the stomach, increasing body fluid, eliminating phlegm, removing stasis, soothing, calming the nerves and so on. With respect to the conditions of hoarse voice, uncomfortable throat and the like, the throat-clearing and sore-throat-relieving candies have the characteristics of quick and good effects, and are free of toxic and side effects. The isomaltitol is utilized as the sweetener of the throat-clearing and sore-throat-relieving candies, and has purer and more natural sweet taste compared with other sweeteners; and the utilization of the isomaltitol also makes the applicable crowd to become wider. The isomaltitol is non-carious, so that the streptococcus mutans in the oral cavity cannot be decomposed and utilized; therefore, no acid and glucan are produced, and no decayed teeth are caused; therefore, the throat-clearing and sore-throat-relieving candies can be eaten by children. Moreover, the throat-clearing and sore-throat-relieving candies do not cause elevation of glycemia and insulin, so that the candies can also be eaten by the patients with diabetes.