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Lactobacillus sialicus and application thereof in down-regulating gene expression of streptococcus mutans biofilm formation

A technology of Lactobacillus salivarius and Streptococcus mutans, applied in the field of microorganisms, can solve the problems of increased risk of drug resistance and low specificity of bactericidal effect

Inactive Publication Date: 2019-01-11
JIAXING INNOCUL PROBIOTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It was found earlier that some strains, such as Lactobacillus rhamnosus and Streptococcus salivarius, can kill oral pathogenic bacteria. However, due to the low specificity of their bactericidal effect, the risk of drug resistance increases after long-term use. Drugs for the prevention and treatment of dental caries with the purpose of sterilization

Method used

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  • Lactobacillus sialicus and application thereof in down-regulating gene expression of streptococcus mutans biofilm formation
  • Lactobacillus sialicus and application thereof in down-regulating gene expression of streptococcus mutans biofilm formation
  • Lactobacillus sialicus and application thereof in down-regulating gene expression of streptococcus mutans biofilm formation

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Embodiment 1

[0031] Lactobacillus salivarius ZM06 is directly isolated from oral samples of healthy people. It is a Gram-positive bacillus that does not form spores and can grow in both aerobic and anaerobic environments.

[0032] Collect the thalli of logarithmic phase of MRS culture, adopt DNA extraction kit to extract DNA, carry out 16SrRNA gene sequencing, its sequence is shown in SEQ ID NO.31, this sequence is compared with GenBank database, can be identified as Lactobacillus salivarius ( Lactobacillus salivarius).

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Abstract

The present invention provides Lactobacillus sialicus and application thereof in down-regulating gene expression of streptococcus mutans biofilm formation, belonging to the field of microbial technology. Lactobacillus salivarius ZM06 according to the present invention is deposited under the accession number CGMCC No. 13978. As the lactobacillus sialicus ZM06 of the invention can normally grow in alysozyme solution with a concentration of 0.2 mg / mL to 1 mg / mL, the oral high lysozyme concentration can be completely tolerate. The lactobacillus sialicus ZM06 of the invention can grow normally ina lysozyme solution with a concentration of 0.2 mg / mL to 1 mg / mL, and thus can tolerate oral high lysozyme concentration. As that lactobacillus sialicus has good tolerance to lysozyme, the lactobacillus sialicus is adapt to oral environment, does not aim to kill streptococcus mutans, but specifically inhibit the formation of streptococcus mutans biofilm by reduce the expression level of genes related to biofilm formation in streptococcus mutans, so as to reduce the occurrence and development risk of dental caries.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Lactobacillus salivarius and its application in down-regulating the gene expression of Streptococcus mutans biofilm formation. Background technique [0002] Dental caries is a prevalent chronic disease in the world. The disease is initiated by the accumulation of plaque biofilm on the surface of teeth, which manifests as damage to hard tissue of teeth and even tooth loss. Dental plaque biofilm is formed by the accumulation of a large number of bacteria, proteins, exopolysaccharides, a small amount of white blood cells, exfoliated epithelial cells and food debris. It has a special spatial structure, and its existence greatly hinders the material exchange inside and outside the plaque. Buffered by saliva, the pH value of the tooth surface drops rapidly; the complex spatial structure of the biofilm also facilitates the exchange of information between bacteri...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/02C12R1/225
CPCA61K2035/115A61P1/02C12N1/205C12R2001/225
Inventor 不公告发明人
Owner JIAXING INNOCUL PROBIOTICS CO LTD
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