Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction method for persimmon tannin

An extraction method and technology of persimmon, applied in the field of extraction of persimmon tannin, to achieve the effect of inhibiting Streptococcus mutans

Inactive Publication Date: 2016-02-24
GUILIN MINGXING BIOTECH
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report that persimmon tannins can be used to inhibit Streptococcus mutans

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Peel and crush the persimmons, add 70v% ethanol 10 times the weight of the persimmons, extract at 60°C for 1 hour, combine the extracts, filter, and take the filtrate for later use;

[0017] 2) Concentrate the filtrate, add 95v% ethanol, the amount of ethanol added is 2 times of the concentrated solution, leave it overnight, centrifuge, and take the supernatant;

[0018] 3) Pass the supernatant through AB-8 macroporous resin, wash the column with water until it is colorless, then elute with ethanol, and collect the ethanol eluate;

[0019] 4) Concentrate the ethanol eluent to recover the solvent to obtain the extract, add water to dissolve, the amount of water added is 3 times the weight of the extract, filter, take the filtrate and pass it through a hollow fiber membrane with a molecular weight cut-off of 450-600, and collect the retentate for later use ;

[0020] 5) passing the retentate through a hollow fiber membrane with a molecular weight cut-off of 14,000-15,...

Embodiment 2

[0022] 1) Peel and crush the persimmons, add 80v% ethanol 20 times the weight of the persimmons, extract 3 times at 80° C. for 3 hours each time, combine the extracts, filter, and take the filtrate for later use;

[0023] 2) Concentrate the filtrate, add 95v% ethanol, the amount of ethanol is 4 times that of the concentrated solution, leave it overnight, centrifuge, and take the supernatant;

[0024] 3) Pass the supernatant through AB-8 macroporous resin, wash the column with water until it is colorless, then elute with ethanol, and collect the ethanol eluate;

[0025] 4) Concentrate the ethanol eluate to recover the solvent to obtain the extract, add water to dissolve it, the amount of water added is 5 times the weight of the extract, filter, take the filtrate and pass it through a hollow fiber membrane with a molecular weight cut-off of 450-600, and collect the retentate for later use ;

[0026] 5) passing the retentate through a hollow fiber membrane with a molecular weigh...

Embodiment 3

[0028] 1) Peel and crush the persimmons, add 75v% ethanol 15 times the weight of the persimmons, extract twice at 70°C for 2 hours each time, combine the extracts, filter, and take the filtrate for later use;

[0029] 2) Concentrate the filtrate, add 95v% ethanol, the amount of ethanol added is 3 times of the concentrated solution, leave it overnight, centrifuge, and take the supernatant;

[0030] 3) Pass the supernatant through AB-8 macroporous resin, wash the column with water until it is colorless, then elute with ethanol, and collect the ethanol eluate;

[0031] 4) Concentrate the ethanol eluate to recover the solvent to obtain the extract, add water to dissolve, the amount of water added is 4 times the weight of the extract, filter, take the filtrate and pass it through a hollow fiber membrane with a molecular weight cut-off of 450-600, and collect the retentate for later use ;

[0032] 5) passing the retentate through a hollow fiber membrane with a molecular weight cut-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method for persimmon tannin. The method comprises the following steps: firstly, persimmons are peeled and crushed, ethanol is added, extraction is carried out, the extracting solution is filtered, and the filtrate is taken for standby; secondly, the filtrate is condensed, 95V% of ethanol is added, the mixture is allowed to stand overnight, centrifugation is carried out, and a supernatant is taken; thirdly, the supernatant is sieved through AB-8 macroporous resin, a column is washed by utilization of water until the column is colorless, thenthe column is washed by utilization of ethanol, and the ethanol eluate is collected; fourthly, the ethanol eluate is condensed, the solvent is recovered, an extract is obtained, the extract is dissolved in water, filtering is carried out, the filtrate is taken and is sieved through a hollow fiber film with a cut-off molecular weight of 450-600, and the cut-off solution is collected for standby; fifthly, the cut-off solution is sieved through a hollow fiber film with a cut-off molecular weight of 14000-15000, the penetration solution is collected and dried, and persimmon tannin power is prepared. The extracted persimmon tannin has a good function of inhibiting streptococcus mutans, and can be used for caries prevention.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting persimmon tannin. Background technique [0002] Persimmon (Persimmon DiospryroskakiL) is rich in tannins in the pulp. Studies have shown that persimmon tannins have strong antioxidant properties and protein-binding capabilities, which can inhibit human lymphocytic leukemia, lower blood pressure, remove free radicals in the body, and significantly inhibit various enzymes in the venom of Agkistrodon halys, cobras, and five-step snakes and toxin activity. Although it has also been reported that persimmon extract, the active ingredients of which are flavonoids, triterpenoids and hydrolyzable tannins, has shown a certain drug resistance to Bacillus subtilis, but the drug resistance is not stable. There is no report that persimmon tannins can be used to inhibit Streptococcus mutans. Contents of the invention [0003] The technical ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H13/08C07H1/08
CPCC07H13/08C07H1/08
Inventor 文继承蒋元勇朱昂辉雍小军易华李丽金
Owner GUILIN MINGXING BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products