Cow mastitis concatenate inactivated vaccine

Abstract

An deactivated multi-bacterium vaccine for preventing the mastitis of milk cow is composed of Staphylococcus aureus, agalactic streptococcus, milk-stopping streptococcus, breast streptococcus, colibacillus and aluminum hydroxide gel. Its preparing process includes such steps as choosing the pathogenic bacterial strain of said mastitis, preparing seed liquid and reproduction liquid, purity checking, deactivating, preparing vaccine, and discriminating.

Description

technical field [0001] The invention relates to a veterinary biological product and a preparation method thereof, more specifically, to a multi-link inactivated bacterium vaccine for preventing cow mastitis and a preparation method thereof. Background technique [0002] Bovine mastitis (Bovine Mastitis) is the inflammation of the mammary gland tissue of dairy cows, which is mainly caused by the infection of pathogenic microorganisms in the mammary gland tissue. It is a common and frequently-occurring disease of dairy cows. According to clinical manifestations, it can be divided into recessive and clinical mastitis. There are about 150 kinds of pathogenic bacteria causing mastitis in cows, mainly Staphylococcus aureus, Streptococcus and Escherichia coli, accounting for more than 90% of the whole cow mastitis cases. Its main features are physical and chemical properties of milk and bacteriological changes, and pathological changes of mammary gland tissue. The most important ...

AI Technical Summary

Problems solved by technology

When mastitis occurs, although there are many cases of mammary gland swelling and pain, most cases of recessive cow mastitis are difficult to find abnormalities when palpated by hand, and pathological changes are also difficult to observe with naked eyes

[0003] At present, the prevention and treatment of mastitis in dairy cows is still mainly based on antibiotic treatment and environmental sanitation control, but antibiotic treatment is often ineffective due to the emergence of drug resistance, resulting in serious antibiotic residues in milk and endangering human health.

In response to this problem, domestic and foreign in recent years have begun to study and use bacterin immunization methods to prevent and control this disease, and have received certain results. For example, Ze'ev Trainin et al. isolated three Bacterial strain, use it to make sterilized vaccine to prevent and treat cattle and sheep mammary Staphylococcus aureus infection (patent application WO99 / 33954), people such as Luo Jinyin ("Chinese Veterinary Science and Technology", 2003,33 (4): 61-63) with gold Staphylococcus aureus, Streptococcus agalactiae and Streptococcus dysgalactiae were mixed in a certain proportion and supplemented with adjuvants to make aluminum glue vaccine, oil emulsion vaccine, propolis vaccine, liposome vaccine and liposome propolis vaccine, which were used in clinical dairy cow immunization Propolis vaccine, oil emulsion vaccine and aluminum glue vaccine all showed certain effects in the vaccination experiment, but the overall immunity level was not high enough