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Truncated recombinant protein of Riemerella anatipestifer (RA) serum type 1 siderophore receptor protein (SRP) and application of truncated recombinant protein

A duck plague Riemer's and receptor protein technology, applied in the field of bioengineering, can solve problems such as the research and application of SRP, which has not been seen in RA serotype 1, and achieve the effects of reducing production costs and increasing solubility

Active Publication Date: 2017-05-10
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

There are no relevant research reports on the research and application of RA serotype 1 SRP

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  • Truncated recombinant protein of Riemerella anatipestifer (RA) serum type 1 siderophore receptor protein (SRP) and application of truncated recombinant protein
  • Truncated recombinant protein of Riemerella anatipestifer (RA) serum type 1 siderophore receptor protein (SRP) and application of truncated recombinant protein
  • Truncated recombinant protein of Riemerella anatipestifer (RA) serum type 1 siderophore receptor protein (SRP) and application of truncated recombinant protein

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Embodiment Construction

[0029] The present invention is further explained by specific examples below, and technologies not described in detail are conventional technologies in the art.

[0030] The amino acid sequence of the RA serum type 1 siderophore receptor protein (SRP) involved in the present invention is shown in SEQ ID NO: 1, and the nucleotide sequence encoding the SRP protein is shown in SEQ ID NO: 2, named as SRP Gene, SRP The length of the gene fragment is 2262bp, which is derived from the whole genome of RA-GD (accession number is CP002562).

[0031] 1. Selection of RA serotype 1 SRP truncated recombinant protein

[0032] Using Signal P and TMHMM online analysis software to analyze SRP The signal peptide sequence, transmembrane sequence and antigenicity analysis of the gene select the amino acid sequence of the RA serum type 1 SRP truncated recombinant protein as shown in SEQ ID NO: 3, and the nucleotide sequence encoding the truncated recombinant protein is as shown in SEQ ID NO: ...

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Abstract

The invention discloses a truncated recombinant protein of a Riemerella anatipestifer (RA) serum type 1 siderophore receptor protein (SRP) and application of the truncated recombinant protein. The SRP truncated recombinant protein can generate specific reaction with a serum type 1 RA clonal antibody, and can be used as an ideal antigen for detecting a serum type 1 RA antibody. The truncated recombinant protein of the RA serum type 1 SRP, prepared by the invention, is not all bacteria, and has no danger of dispersing toxicity; in addition, the solubility of the protein during prokaryotic expression is increased, and the large-scale production cost of downstream can be effectively reduced; the truncated recombinant protein is suitable for industrial large-scale production and facilitates the research on laboratory vaccines.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and more specifically relates to a truncated recombinant protein of Riemerella anatipestifer serum type 1 siderophore receptor protein SRP and its application. Background technique [0002] Riemerella anatipestifer (RA) is one of the highly pathogenic and contagious diseases of ducks, geese, turkeys and other birds. Violation of 1 to 8 weeks old (especially 2 to 3 weeks old) ducklings, goslings and young turkeys. Riemerella anatipestifer disease is mostly an acute or chronic sepsis course, mainly characterized by neurological symptoms, fibrinous pericarditis, perihepatitis and air sacculitis, with an incidence rate of over 90% and a mortality rate of up to 75%. Ducks often grow into defective ducks or stiff ducks, with reduced feed conversion rate and stunted growth, and salpingitis caused by RA seriously affects the egg production rate of ducks as adults. Therefore, the widespread preva...

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Application Information

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IPC IPC(8): C07K14/195C12N15/31C12N15/70G01N33/68G01N33/569C12R1/01C12R1/19
CPCC07K14/195C12N15/70C12N2800/101G01N33/56911G01N33/6854G01N2469/20
Inventor 董嘉文李林林袁建丰孙敏华
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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