Specific LAMP primers, kit and method for detecting streptococcus uberis

A technology of Streptococcus uberis and a kit, applied in the field of microbial detection, can solve the problems of long time, expensive equipment, complicated operation and the like

Pending Publication Date: 2020-07-07
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of classic methods such as cumbersome operation, long time-consuming, and low detection rate, it affects the rapid and accurate detection of the bacteria and the treatment of mastitis caused by it; the existing polymerase chain reaction (polymerase chain reaction, PCR ) technologies inc

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  • Specific LAMP primers, kit and method for detecting streptococcus uberis
  • Specific LAMP primers, kit and method for detecting streptococcus uberis
  • Specific LAMP primers, kit and method for detecting streptococcus uberis

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Embodiment Construction

[0021] The following examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0022] The invention provides a visual LAMP kit for detecting Streptococcus uberis with strong specificity, high sensitivity, low requirements on instruments and equipment, fast and simple operation and low cost, so as to meet the needs of rapid on-site detection of Streptococcus uberis at the grassroots level.

[0023] The first object of the present invention is to provide a specific LAMP detection primer set for Streptococcus uberis.

[0024] The second object of the present invention is to provide a visual LAMP detection kit for Streptococcus uberis.

[0025] The third object of the present invention is to provide a visual LAMP detection method for Streptococcus uberis. It not only reduces the cost of detection, but also improves the accuracy of detection.

[0026]The present invention adopts the following ...

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Abstract

The present invention discloses specific LAMP primers, kit and method for detecting streptococcus uberis. The kit comprises the six LAMP primers including outer primers F3 and B3, inner primers FIP and BIP and loop primers LB and LF, and the six primers aim at conserved sequence design of a streptococcus uberis KX389960. 1 gene; and a nucleotide sequence of FIP is as shown in SEQ ID NO.1, a nucleotide sequence of BIP is as shown in SEQ ID NO.2, a nucleotide sequence of F3 is as shown in SEQ ID NO.3, a nucleotide sequence of B3 is as shown in SEQ ID NO.4, a nucleotide sequence of LB is as shownin SEQ ID NO.5, and a nucleotide sequence of LF is as shown in SEQ ID NO.6. The kit solves the problems that an existing breast streptococcus detection technology has the long period and the high detection cost and cannot be applied to on-site rapid detection.

Description

technical field [0001] The invention relates to the field of microorganism detection, more specifically, to a specific LAMP primer, a kit and a method for detecting Streptococcus uberis. Background technique [0002] Streptococcus uberis (Streptococcus uberis) is one of the most common pathogens causing mastitis in dairy cows. It can cause clinical and subclinical mastitis in cows, cause chronic infection of mammary gland tissue, and is highly contagious, eventually leading to low milk production in cows. decline or even stop lactation. Seriously restrict the development of dairy farming and dairy processing, and endanger public health and human health. [0003] Usually, the detection of Streptococcus uberis in clinical samples is still through classical methods such as bacterial isolation, biochemical tests and CAMP tests. However, due to the shortcomings of classic methods such as cumbersome operation, long time-consuming, and low detection rate, it affects the rapid and...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12Q1/6844C12N15/11C12R1/46
CPCC12Q1/6844C12Q1/689C12Q2531/119
Inventor 邓光存马臣杰吴晓玲王雯雯
Owner NINGXIA UNIVERSITY
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