53results about How to "Broad cracking spectrum" patented technology

The invention discloses a bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof. The invention can obtain bdellovibrio bacteriovorus BDFM05 by separationand ultraviolet mutation. The bdellovibrio bacteriovorus BDFM05 is combined with oligosaccharide to obtain bdellovibrio bacteriovorus for preventing and curing crop bacterial diseases. The inventionapplies bdellovibrio bacteriovorus BDFM05 to prevent and cure crop bacterial diseases for the first time. The bdellovibrio bacteriovorus prepared by the method has better rice bacterial diseases preventing and curing effect than common Saisentong, has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, produces no pathogenic bacteria resistance, has no toxic or side effects to people and animals, improves the quality and safety of agricultural products and conforms to the requirements of people on healthy and green food and sustainable-development for agriculturalproduction.

The invention discloses a strong lysis bacteriophage for splitting NDM-1 positive Klebsiella pneumoniae clinical isolates, and an application of the strong lysis bacteriophage used as a biological preparation in the prevention and treatment of NDM-1 Klebsiella pneumoniae induced infection and pollution. The preservation number of the bacteriophage is CCTCC M 2015760, and the bacteriophage has a regularly icosahedral head and a telescopic tail, and belongs to a Myoviridae double-stranded DNA bacteriophage. The bacteriophage has strong kilign activity and wide bacteriophage lysis spectrum, can be used for preparing medicines for preventing and treating NDM-1 Klebsiella pneumoniae induced bacterium infection, and also can be used for killing NDM-1 positive Klebsiella pneumoniae in culture environment and medical environment.

The invention provides a staphylococcus aureus phage strain VB_SavM_JYL01. The staphylococcus aureus phage stain VB_SavM_JYL01 has been preserved in the China Center for Type Culture Collection on 25th March, 2018, the preservation name is Staphylococcus aureus phage VB_SavM_JYL01, and the preservation number is CCTCC M 2018154. The staphylococcus aureus phage strain has a wide lysis spectrum, thus staphylococcus aureus can be lysed, certain staphylococcus epidermidis can also be lysed, host bacteria 206 can be lysed, and staphylococcus clinical strains separated from the environment or fecesof diseased animals can also be lysed; the staphylococcus aureus phage can be used independently or in combination with other substances, and a safe and nontoxic phage disinfecting and killing productis provided for disinfecting and purifying the environment.

The invention relates to the field of microorganisms, in particular to a ralstonia solanacearum phage GP1 with the preservation number of CCTCC M 2016633, a ralstonia solanacearum phage GP2 with the preservation number of CCTCC M 2016634 and a ralstonia solanacearum phage GP3 with the preservation number of CCTCC M 2016635. The phages have the advantages that the phages are strict virulent phagesand can effectively kill ralstonia solanacearum of different plant host sources, especially the ralstonia solanacearum causing ginger blast; the phages are non-toxic to normal microbial flora, DNAs ofthe phages cannot encode virulence genes, and the stability is high under the pH of 5-9; there is no magnitude change in titer at 4 DEG C after preservation for one year; bacteria can be effectivelyinhibited and killed under low titer. The phages can be used for prevention and treatment of crop bacterial wilt caused by ralstonia solanacearum, especially ginger blast, excellent strain resources are provided for developing novel bio-pesticide resistant to bacterial wilt, and a good application and development prospect is achieved.

The invention discloses a bactericide for preventing and curing crop bacterial diseasess as well as a preparation method and application thereof. In the preparation method, oligosaccharide is added into bdellovibrio bacteriovorus solution to be evenly mixed to obtain the bactericide for preventing and curing crop bacterial diseasess. The invention applies bdellovibrio bacteriovorus solution for preventing and curing crop bacterial diseases for the first time, fills the vacancy of researching and developing bdellovibrio bacteriovorus at home and abroad and adds new species for biopesticide. Asbdellovibrio bacteriovorus has the characteristics of broad cracking pattern and quick growth and has the outstanding capability of eliminating various pathogenicbacteria and bacteria, after combinedwith oligosaccharide, the bdellovibrio bacteriovorus strengthens crop resistance, greatly improves pesticide effect and enlarges the preventing and curing spectrum of bacterial diseases. The bactericide prepared by the invention has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, pathogenic bacteria have small possibility of producing resistance, so that the invention overcomes 3R problems brought by abusing antibiotics and avoids the defects of serious chemical pesticide pollution and ecological balance damage at present.

The invention belongs to the field of biotechnology, and discloses a pseudomonas aeruginosa bacteriophage and an application thereof in prevention of hemorrhagic pneumonia of a mink. The pseudomonas aeruginosa bacteriophage is preserved in China General Microbiological Culture Collection Center on April 14, 2014, has the preservation number of CGMCC No. 9102, and is classified and named pseudomonas aeruginosa bacteriophage. As for the application of the pseudomonas aeruginosa bacteriophage in prevention of hemorrhagic pneumonia of the mink, assume that the living space of each mink is 1m<3>; an ultrasonic spraying mode is adopted; the titer of the pseudomonas aeruginosa bacteriophage is 10<9>PFU / ml, and the dose for each mink is 10ml; hemorrhagic pneumonia of the mink can be prevented by using the pseudomonas aeruginosa bacteriophage once per month in a disease outbreak period. By adopting the ultrasonic spraying application mode, the bacteriophage can directly arrive in the lung where bacteria is colonized, thus improving the efficiency of controlling bacterial infection of the lung of an animal by the bacteriophage, and improving the breeding economic benefits.

The invention provides a method for establishing a model of inhibiting mastitis in mice by Klebsiella pneumoniae phage. The method comprises the following steps: A, preparation of a Klebsiella pneumoniae bacterial liquid; B, preparation of a Klebsiella pneumoniae phage culture solution; C, treatment of an experimental animal; and D, collection and testing of experimental samples. Through the method, the Klebsiella pneumoniae phage with wide lysis spectrum, strong bacterium lysis ability and no toxic side effects for mastitis in mice can be more accurately found, and thereby the experimental and theoretical basis for the treatment of bovine mastitis is provided.

The invention relates to the technical field of microorganisms, and particularly relates to a Staphylococcus aureus phage and bacteriostatic application thereof. The Staphylococcus aureus phages107 is sent to China Center for Type Culture Collection for Preservation, the classification name is Staphylococcus aureus phages107, the preservation number is CCTCC NO: M2021125, the preservation date is January 21, 2021, and the address is Wuhan University, Wuhan City, China. The phages107 has high titer up to 2.31 * 10<10>PFU / mL, good pH stability, good temperature stability and wide lysis spectrum, and has lysis ability on 9 strains of Staphylococcus aureus, wherein the 9 strains of Staphylococcus aureus have certain drug resistance and no lysis ability on escherichia coli. The Staphylococcus aureus phage has specific lysis spectrum for Staphylococcus aureus, and has strong lysis ability. The Staphylococcus aureus phages107 can be used for preparation of drugs for treating Staphylococcus aureus induced dairy cow mastitis and other diseases.

The invention belongs to the technical field of microorganism prevention and treatment, and particularly relates to a vibrio parahaemolyticus bacteriophage VP-HYP MCS-1 and bdellovibrio bacteriovorusHalobacterovoraxsp.MCS-1 composition and application thereof to prevention and treatment of prawn pathogenic vibrio parahaemolyticus infection. The vibrio parahaemolyticus bacteriophage is vibrio parahaemolyticus bacteriophage VP-HYPMCS-1, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.199693. The vibrio parahaemolyticus bacteriophage is a vibrio parahaemolyticus bacteriophage VP-HYPMCS-1. The bdellovibrio parahaemolyticus is bdellovibrio parahaemolyticus Halobacterovoraxsp.MCS-1, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.199694. The invention also discloses a preparation method of the bdellovibrio parahaemolyticus strain. The virulent bacteriophage VP-HYP MCS-1 obtained through separation is high in host specificity and has cracking and killing effects on vibrio parahaemolyticus; the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is obtained through separation, the host spectrum of the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is wide, and the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 has cracking and killing effects on various vibrios including vibrio parahaemolyticus; the vibrio parahaemolyticus bacteriophage and bdellovibrio bacteriovorus provided by the invention have good application prospects in prevention and control of prawn pathogenic vibrio parahaemolyticus infection.

The invention discloses a clostridium welchii bacteriophage as well as a bacteriophage composition and application thereof, the bacteriophage is a clostridium welchii bacteriophage PMQ06 and is preserved in China General Microbiological Culture Collection Center on May 15, 2020, and the preservation number is CGMCC No.19977. The bacteriophage has the advantages of high-efficiency bactericidal activity, wide lysis spectrum and high temperature stability, can be used as an active ingredient for preparing drugs for preventing and treating chicken clostridium welchii disease, environmental disinfectants, feed additives, bacteriostats and the like, can be widely applied to various links in the breeding process and the product processing and production process, and has broad market prospects. The pathogenicity clostridium welchii and even the drug-resistant clostridium welchii are rapidly, efficiently and safely killed, the mortality rate of morbidity chickens is effectively reduced, and thefeed additive has the advantages of being safe, free of residues and free of side effects.

The invention provides lyase capable of killing streptococcus equi subspecies. Equi and medical application, and provides a new bacteriophage lyase which can be independently used or compounded with other substances for use, has strong lytic activity and a wide lytic spectrum, can effectively kill streptococcus equi subsp. Equi and streptococcus suis. The invention provides a novel medicine for preventing and treating diseases caused by streptococcus equi subsp. Equi and streptococcus suis infection.

The invention discloses an FSN17-1 Photobacterium damselae phage FSN17-1 and application reserved in China Center for Type Culture collection, and the preservation number is CCTCC NO: M2018393; the FSN17-1 Photobacterium damselae phage can stably survive under the condition that the temperature is 30-60 DEG C and the pH is 5.0-9.0, the hydrophily is good, the splitting spectrum is wide, and the FSN17-1 Photobacterium damselae phage has the good splitting capability for Photobacterium damselae, has the good treatment effect on infection of Photobacterium damselae, and a novel way is provided for pathogenic bacterium prevention and control.

The invention discloses pyrolysis phycophage MACPNOA1 and a separation method and application thereof, and relates to biological treatment of eutrophic water. MACPNOA1 is preserved in China General Microbiological Culture Collection Center in October 24th, 2016, and the preservation number is CGMCC No.12996. MACPNOA1 has a wide alge-lysing spectrum, and widely lyses common water-blooming cyanobacteria; when MACPNOA1 is added into cyanobacterial water-blooming liquid belonging to seven categories of Microcystis, Aphanizomenon, Chroococcus, Planktothrix, Nostoc, Oscillatoria and Anabaena, MACPNOA1 has strong pyrolysis effects on all the measured seven categories. When MACPNOA1 is applied to algal bloom treatment, the obvious effect of protecting animals in water is achieved. The pyrolysis phycophage MACPNOA1 has the advantages of being safe, efficient and quick.

The invention provides a lyase plssX609 for lysing Gram-positive bacteria and application thereof, and particularly relates to the field of genetic engineering. The invention provides a lyase glyssX609 for lysing Gram-positive bacteria, and the lyase glyssX609 is characterized in that the nucleotide sequence of the lyase glyssX609 is as shown in SEQ ID NO.1. The lyase lyssX609 provided by the invention has the advantages of strong stability and high lysing efficiency, and has a lysing effect on a plurality of Gram-positive bacteria.

The invention discloses a strain of acinetobacter baumannii bacteriophage. The acinetobacter baumannii bacteriophage is a novel bacteriophage with cytotoxicity, deposited in the China Center for TypeCulture Collection on September 9, 2019 and named as acinetobacter baumannii bacteriophage VB_AbaM_DC3 with preservation number of CCTCC NO: M 2019705. The invention further provides a medical application of the acinetobacter baumannii bacteriophage. The bacteriophage has a wider lysis spectrum and can lyse 10 different clinical isolates of acinetobacter baumannii. The bacteriophage can be used alone or in combination with acinetobacter baumannii bacteriophages other than the bacteriophage of the present invention or other disinfecting and sterilizing substances to reduce numbers of the acinetobacter baumannii in intensive care units (ICU) and general wards in hospitals, thereby reducing incidence of nosocomial infection.

The invention belongs to the technical field of microorganisms, and relates to a riemerella anatipestifer phage and application thereof. The riemerella anatipestifer bacteriophage provided by the invention is RA-XHX1, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20241268, and is classified and named as the riemerella anatipestifer bacteriophage. The bacteriophage is double-stranded DNA (Deoxyribose Nucleic Acid) and myuroviridae, can form transparent plaques on a solid culture medium, has no halo ring around and is clear and regular in edge; the bacteriophage has a relatively good killing effect on riemerella anatipestifer in a space environment, particularly high-pathogenicity riemerella anatipestifer, and can realize a broad-spectrum antibacterial effect when being applied to preparation of medicines for preventing and treating riemerella anatipestifer infection.

The invention discloses a bacteriophage. DNA (deoxyribonucleic acid) sequences of the bacteriophage are sequences shown as SEQ ID No.1. The bacteriophage has the advantages that the bacteriophage is broad in lysis spectrum, and different types of vibrio parahaemolyticus can be lysed by the bacteriophage; the bacteriophage is high in heat stability and can tolerate high temperatures in environments; the numbers of bacteria in samples can be obvious reduced in the short time after the fish samples contaminated by the vibrio parahaemolyticus are treated by the bacteriophage, accordingly, the factthat the vibrio parahaemolyticus in the samples can be killed by the bacteriophage is illustrated, effects of food safety protective agents can be realized by the bacteriophage, and the bacteriophagehas a broad application prospect.

The invention belongs to the technical field of bacteriophages, and discloses a salmonella bacteriophage with cross-species lysis capability, a bacteriophage composition and application of the salmonella bacteriophage, the salmonella bacteriophage is named as salmonella bacteriophage PC553, the preservation number of the salmonella bacteriophage PC553 is CGMCC No.46166, and the preservation The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 16, 2024, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacteriophage is wide in splitting spectrum and high in titer, shows cross-species strong splitting energy to escherichia coli, can be prepared into drugs, environment disinfectants, feeds, water additives and the like for preventing and treating pigeon salmonellosis and / or colibacillosis for application, effectively prevents and treats spreading of salmonellosis and avoids the problem of secondary infection of escherichia coli, and has a good application prospect in preventing and treating pigeon salmonellosis and / or colibacillosis. The feed additive has the effects of effectively reducing the death and culling rate of pigeons, reducing the positive rate of salmonellosis antibodies, improving the production performance index of pigeons and the like.

The invention discloses a separation and screening method based on Listeria monocytogenes bacteriophage, and relates to the technical field of bioengineering. Bacteriophage separation is carried out according to untreated slaughter sewage of a live pig slaughter house, and the separation method based on the Listeria monocytogenes bacteriophage comprises the following steps: preparing materials and reagents; performing sample treatment; and separating and screening the bacteriophage. The lysing LM bacteriophage is separated from nature and is subjected to morphological observation; the titer, the one-step growth curve and the optimal infection complex number of the bacteriophage are measured, a host spectrum is determined; the structural basis, the phagocytosis ability and the phagocytosis specificity of the lysing LM bacteriophage function are known, and standardized preparation of samples acting on subsequent clinical research is achieved. An optimal preservation method of the bacteriophage is explored, and a data basis is provided for subsequent gene modification, induced mutation and protein expression by extracting genomes for sequencing and researching action genes and structural genes of the bacteriophage.

The invention provides a method for establishing a Klebsiella pneumoniae phage inhibiting mouse mastitis model, comprising the following steps: A, preparation of Klebsiella pneumoniae bacterial liquid, B, preparation of Klebsiella pneumoniae phage culture liquid, C, Experimental animal treatment, D, collection and testing of experimental samples. Through this method, the present invention can more accurately find Klebsiella pneumoniae phages with wide lysis spectrum for mouse mastitis, strong bacteriostasis ability and no toxic or side effects, so as to provide experimental and theoretical basis for treating bovine mastitis.

The invention discloses a highly lytic Salmonella phage RDP‑SA‑19009, the preservation number is CGMCCNo.21993, and the gene length is 87591bp. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes; Under the high temperature condition of ℃ for 30min, the titer remains at 1.0×10 6 More than pfu / mL, it has higher tolerance to temperature; the Salmonella phage RDP-SA-19009 provided by the present invention can crack including Salmonella typhimurium, Salmonella Stanley, Salmonella enteritidis, Salmonella Kentucky, Salmonella typhi, Salmonella Shenfudenburg Pathogenic bacteria, including Salmonella, have the characteristics of wide lysis spectrum and high lysis rate. The phage can be used alone or in combination with other Salmonella phages and probiotics, which can effectively treat and prevent poultry salmonellosis .

The application discloses a bacillus cereus phage for treating high-pathogenicity bacillus cereus infection of Chinese soft-shelled turtles, which is named PSYJ-YH in Latin, is deposited in the Guangdong Microbial Culture Collection Center on October 25, 2023, and has a preservation number of GDMCC No.63912-B1. Bacillus cereus The application has a strong lysis effect on multiple strains of high-pathogenicity bacillus cereus and can be used for preventing and controlling high-pathogenicity bacillus cereus in a breeding environment or an animal body.

The invention provides bacillus subtilis BS-21001 and application thereof, belongs to the technical field of biological prevention and treatment, and can solve the technical problems that a traditional riemerella anatipestifer disease treatment method is not ideal in immune effect and drug residues exist. The bacillus subtilis BS-21001 is named as bacillus subtilis in taxonomy, the preservation number of the bacillus subtilis BS-21001 is CGMCC (China General Microbiological Culture Collection Center) No.26444, and the bacillus subtilis BS-21001 is preserved in the China General Microbiological Culture Collection Center on March 23, 2023. The fermentation supernatant of the bacillus subtilis BS-21001 can be used as a single active component or compounded with other active components to prepare a microecological preparation, and the microecological preparation can effectively inhibit riemerella anatipestifer in duck bodies and in a breeding environment and has no chemical pollution to the environment.

The invention discloses a stable and efficient Salmonella virulent phage RDP-SA-21004, the preservation number is CGMCC No. 22483. After the whole gene is sequenced, it is found that it does not have virulence genes and lysogenic genes; Stored for 10 months under conditions, its activity remains at 10 8 Above pfu / mL, it has strong stability; it can efficiently infect host bacteria, and its optimal multiplicity of infection is 0.1; it has a broad spectrum of ability to kill Salmonella, and also has a certain ability to lyse Escherichia coli. The phage can be used alone or in combination with other Salmonella phages and probiotics, which can effectively treat, prevent and reduce poultry salmonellosis.

The present invention discloses a Salmonella phage RDP-SA-21003 capable of lysing Escherichia coli at the same time, and the preservation number is CGMCC No.22494. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes; the present invention obtains The lysing efficiency of the phage to the host bacterium BS20078 is above 98.7%. When used alone, the lysing ability to Salmonella is relatively strong and the lysing spectrum is wide; With cracking ability.

The invention discloses an application of calcium ions in promoting the formation of marine bdellovibrio bdelloplasts, and belongs to the technical field of bdellovibrio. The present invention promotes the formation of Bdellovrio sp. Bdellovrio sp. BDE‑1 high-density Bdellovrio sp. Bdellovrio sp. by utilizing calcium ions, the factor that promotes the formation of Bdellovibrio leech plastids; The formation of hiruplasma all has obvious promoting effect, and the cracking performance of hiruplasma has not declined, and the shelf life of high-density hiruplasma group has been obviously prolonged compared with control group; The Vibrio that causes human infection and food poisoning can also inhibit the Vibrio that can cause biological diseases in aquaculture. At the same time, it also has a good antibacterial effect on other food-borne pathogens. It has a wide cracking spectrum and its cracking Compared with the mixture of Bdellovibrio BDE-1, the effect remains unchanged; the method for promoting the cultivation of the bdeloid plastids of the present invention is simple and easy, and can be popularized and used.

The invention discloses a campylobacter coli bacteriophage and application thereof, and relates to the technical field of biology. The campylobacter coli bacteriophage is preserved in the China Center for Type Culture Collection on September 25, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO.M 20242066. The invention further discloses a preparation method of the campylobacter coli bacteriophage. The campylobacter coli bacteriophage is separated from an excrement sample of a poultry farm in Hubei province, and basic characterization and application effect evaluation are carried out on the bacteriophage through whole genome sequencing, electron microscope observation, biological characteristic experiments and the like. Results show that the bacteriophage not only is fast in replication and easy to amplify, but also has good stable activity. The bacteriophage has a wide lysis spectrum, can effectively inhibit proliferation of campylobacter, can be developed into antibacterial products, and has important market application value.

The invention belongs to the technical field of microbial control, and in particular relates to a combination of Vibrio parahaemolyticus phage VP-HYP MCS-1 and Halobacteriovorax sp.MCS-1 and their use in preventing and treating pathogenic Vibrio parahaemolyticus infection of prawns. application. The phage of Vibrio parahaemolyticus is the phage of Vibrio parahaemolyticus VP-HYP MCS-1, which has been deposited in the General Microbiology Center of the China Microorganism Culture Collection and Administration Commission, and the preservation number is CGMCC No.19693. Vibrio parahaemolyticus is Vibrio parahaemolyticus Halobacteriovorax sp.MCS‑1, which has been deposited in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee, and the preservation number is CGMCC No.19694. The virulent bacteriophage VP-HYP MCS-1 isolated and obtained by the present invention has strong host specificity and has the functions of lysing and killing Vibrio parahaemolyticus; the isolated and obtained leech vibrio Halobacteriovorax sp.MCS-1 has a wide host spectrum , has the effect of lysing and killing a variety of Vibrio including Vibrio parahaemolyticus; the Vibrio parahaemolyticus bacteriophage and leech vibrio provided by the present invention have good performance in preventing and controlling pathogenic Vibrio parahaemolyticus infection of prawns application prospects.