44results about How to "Broad cracking spectrum" patented technology

The invention discloses a bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof. The invention can obtain bdellovibrio bacteriovorus BDFM05 by separationand ultraviolet mutation. The bdellovibrio bacteriovorus BDFM05 is combined with oligosaccharide to obtain bdellovibrio bacteriovorus for preventing and curing crop bacterial diseases. The inventionapplies bdellovibrio bacteriovorus BDFM05 to prevent and cure crop bacterial diseases for the first time. The bdellovibrio bacteriovorus prepared by the method has better rice bacterial diseases preventing and curing effect than common Saisentong, has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, produces no pathogenic bacteria resistance, has no toxic or side effects to people and animals, improves the quality and safety of agricultural products and conforms to the requirements of people on healthy and green food and sustainable-development for agriculturalproduction.

The invention relates to the field of microorganisms, in particular to a ralstonia solanacearum phage GP1 with the preservation number of CCTCC M 2016633, a ralstonia solanacearum phage GP2 with the preservation number of CCTCC M 2016634 and a ralstonia solanacearum phage GP3 with the preservation number of CCTCC M 2016635. The phages have the advantages that the phages are strict virulent phagesand can effectively kill ralstonia solanacearum of different plant host sources, especially the ralstonia solanacearum causing ginger blast; the phages are non-toxic to normal microbial flora, DNAs ofthe phages cannot encode virulence genes, and the stability is high under the pH of 5-9; there is no magnitude change in titer at 4 DEG C after preservation for one year; bacteria can be effectivelyinhibited and killed under low titer. The phages can be used for prevention and treatment of crop bacterial wilt caused by ralstonia solanacearum, especially ginger blast, excellent strain resources are provided for developing novel bio-pesticide resistant to bacterial wilt, and a good application and development prospect is achieved.

The invention discloses a bactericide for preventing and curing crop bacterial diseasess as well as a preparation method and application thereof. In the preparation method, oligosaccharide is added into bdellovibrio bacteriovorus solution to be evenly mixed to obtain the bactericide for preventing and curing crop bacterial diseasess. The invention applies bdellovibrio bacteriovorus solution for preventing and curing crop bacterial diseases for the first time, fills the vacancy of researching and developing bdellovibrio bacteriovorus at home and abroad and adds new species for biopesticide. Asbdellovibrio bacteriovorus has the characteristics of broad cracking pattern and quick growth and has the outstanding capability of eliminating various pathogenicbacteria and bacteria, after combinedwith oligosaccharide, the bdellovibrio bacteriovorus strengthens crop resistance, greatly improves pesticide effect and enlarges the preventing and curing spectrum of bacterial diseases. The bactericide prepared by the invention has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, pathogenic bacteria have small possibility of producing resistance, so that the invention overcomes 3R problems brought by abusing antibiotics and avoids the defects of serious chemical pesticide pollution and ecological balance damage at present.

The invention belongs to the field of biotechnology, and discloses a pseudomonas aeruginosa bacteriophage and an application thereof in prevention of hemorrhagic pneumonia of a mink. The pseudomonas aeruginosa bacteriophage is preserved in China General Microbiological Culture Collection Center on April 14, 2014, has the preservation number of CGMCC No. 9102, and is classified and named pseudomonas aeruginosa bacteriophage. As for the application of the pseudomonas aeruginosa bacteriophage in prevention of hemorrhagic pneumonia of the mink, assume that the living space of each mink is 1m<3>; an ultrasonic spraying mode is adopted; the titer of the pseudomonas aeruginosa bacteriophage is 10<9>PFU/ml, and the dose for each mink is 10ml; hemorrhagic pneumonia of the mink can be prevented by using the pseudomonas aeruginosa bacteriophage once per month in a disease outbreak period. By adopting the ultrasonic spraying application mode, the bacteriophage can directly arrive in the lung where bacteria is colonized, thus improving the efficiency of controlling bacterial infection of the lung of an animal by the bacteriophage, and improving the breeding economic benefits.

The invention provides a method for establishing a model of inhibiting mastitis in mice by Klebsiella pneumoniae phage. The method comprises the following steps: A, preparation of a Klebsiella pneumoniae bacterial liquid; B, preparation of a Klebsiella pneumoniae phage culture solution; C, treatment of an experimental animal; and D, collection and testing of experimental samples. Through the method, the Klebsiella pneumoniae phage with wide lysis spectrum, strong bacterium lysis ability and no toxic side effects for mastitis in mice can be more accurately found, and thereby the experimental and theoretical basis for the treatment of bovine mastitis is provided.

The invention belongs to the technical field of microorganism prevention and treatment, and particularly relates to a vibrio parahaemolyticus bacteriophage VP-HYP MCS-1 and bdellovibrio bacteriovorusHalobacterovoraxsp.MCS-1 composition and application thereof to prevention and treatment of prawn pathogenic vibrio parahaemolyticus infection. The vibrio parahaemolyticus bacteriophage is vibrio parahaemolyticus bacteriophage VP-HYPMCS-1, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.199693. The vibrio parahaemolyticus bacteriophage is a vibrio parahaemolyticus bacteriophage VP-HYPMCS-1. The bdellovibrio parahaemolyticus is bdellovibrio parahaemolyticus Halobacterovoraxsp.MCS-1, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.199694. The invention also discloses a preparation method of the bdellovibrio parahaemolyticus strain. The virulent bacteriophage VP-HYP MCS-1 obtained through separation is high in host specificity and has cracking and killing effects on vibrio parahaemolyticus; the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is obtained through separation, the host spectrum of the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is wide, and the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 has cracking and killing effects on various vibrios including vibrio parahaemolyticus; the vibrio parahaemolyticus bacteriophage and bdellovibrio bacteriovorus provided by the invention have good application prospects in prevention and control of prawn pathogenic vibrio parahaemolyticus infection.

The invention discloses a clostridium welchii bacteriophage as well as a bacteriophage composition and application thereof, the bacteriophage is a clostridium welchii bacteriophage PMQ06 and is preserved in China General Microbiological Culture Collection Center on May 15, 2020, and the preservation number is CGMCC No.19977. The bacteriophage has the advantages of high-efficiency bactericidal activity, wide lysis spectrum and high temperature stability, can be used as an active ingredient for preparing drugs for preventing and treating chicken clostridium welchii disease, environmental disinfectants, feed additives, bacteriostats and the like, can be widely applied to various links in the breeding process and the product processing and production process, and has broad market prospects. The pathogenicity clostridium welchii and even the drug-resistant clostridium welchii are rapidly, efficiently and safely killed, the mortality rate of morbidity chickens is effectively reduced, and thefeed additive has the advantages of being safe, free of residues and free of side effects.

The invention provides lyase capable of killing streptococcus equi subspecies. Equi and medical application, and provides a new bacteriophage lyase which can be independently used or compounded with other substances for use, has strong lytic activity and a wide lytic spectrum, can effectively kill streptococcus equi subsp. Equi and streptococcus suis. The invention provides a novel medicine for preventing and treating diseases caused by streptococcus equi subsp. Equi and streptococcus suis infection.

The invention discloses an FSN17-1 Photobacterium damselae phage FSN17-1 and application reserved in China Center for Type Culture collection, and the preservation number is CCTCC NO: M2018393; the FSN17-1 Photobacterium damselae phage can stably survive under the condition that the temperature is 30-60 DEG C and the pH is 5.0-9.0, the hydrophily is good, the splitting spectrum is wide, and the FSN17-1 Photobacterium damselae phage has the good splitting capability for Photobacterium damselae, has the good treatment effect on infection of Photobacterium damselae, and a novel way is provided for pathogenic bacterium prevention and control.

The invention discloses pyrolysis phycophage MACPNOA1 and a separation method and application thereof, and relates to biological treatment of eutrophic water. MACPNOA1 is preserved in China General Microbiological Culture Collection Center in October 24th, 2016, and the preservation number is CGMCC No.12996. MACPNOA1 has a wide alge-lysing spectrum, and widely lyses common water-blooming cyanobacteria; when MACPNOA1 is added into cyanobacterial water-blooming liquid belonging to seven categories of Microcystis, Aphanizomenon, Chroococcus, Planktothrix, Nostoc, Oscillatoria and Anabaena, MACPNOA1 has strong pyrolysis effects on all the measured seven categories. When MACPNOA1 is applied to algal bloom treatment, the obvious effect of protecting animals in water is achieved. The pyrolysis phycophage MACPNOA1 has the advantages of being safe, efficient and quick.

The invention provides a lyase plssX609 for lysing Gram-positive bacteria and application thereof, and particularly relates to the field of genetic engineering. The invention provides a lyase glyssX609 for lysing Gram-positive bacteria, and the lyase glyssX609 is characterized in that the nucleotide sequence of the lyase glyssX609 is as shown in SEQ ID NO.1. The lyase lyssX609 provided by the invention has the advantages of strong stability and high lysing efficiency, and has a lysing effect on a plurality of Gram-positive bacteria.

The invention discloses a strain of acinetobacter baumannii bacteriophage. The acinetobacter baumannii bacteriophage is a novel bacteriophage with cytotoxicity, deposited in the China Center for TypeCulture Collection on September 9, 2019 and named as acinetobacter baumannii bacteriophage VB_AbaM_DC3 with preservation number of CCTCC NO: M 2019705. The invention further provides a medical application of the acinetobacter baumannii bacteriophage. The bacteriophage has a wider lysis spectrum and can lyse 10 different clinical isolates of acinetobacter baumannii. The bacteriophage can be used alone or in combination with acinetobacter baumannii bacteriophages other than the bacteriophage of the present invention or other disinfecting and sterilizing substances to reduce numbers of the acinetobacter baumannii in intensive care units (ICU) and general wards in hospitals, thereby reducing incidence of nosocomial infection.

The invention discloses a separation and screening method based on Listeria monocytogenes bacteriophage, and relates to the technical field of bioengineering. Bacteriophage separation is carried out according to untreated slaughter sewage of a live pig slaughter house, and the separation method based on the Listeria monocytogenes bacteriophage comprises the following steps: preparing materials and reagents; performing sample treatment; and separating and screening the bacteriophage. The lysing LM bacteriophage is separated from nature and is subjected to morphological observation; the titer, the one-step growth curve and the optimal infection complex number of the bacteriophage are measured, a host spectrum is determined; the structural basis, the phagocytosis ability and the phagocytosis specificity of the lysing LM bacteriophage function are known, and standardized preparation of samples acting on subsequent clinical research is achieved. An optimal preservation method of the bacteriophage is explored, and a data basis is provided for subsequent gene modification, induced mutation and protein expression by extracting genomes for sequencing and researching action genes and structural genes of the bacteriophage.