Vibrio parahaemolyticus bacteriophage, bdellovibrio bacteriovorus and application thereof

A technology of Vibrio hemolyticus and bacteriophage, applied in the direction of bacteriophage, virus/phage, application, etc., can solve the problems of long reproduction time and resistance mutation of leech vibrio, and achieve strong host specificity, long-lasting curative effect, and wide lysis spectrum. Effect

Active Publication Date: 2020-08-18
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF10 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the current disadvantages of single phage infection that easily produces resistance mutations and long breeding time of Bdellovibrio, the purpose of the present invention is to provide a combinat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vibrio parahaemolyticus bacteriophage, bdellovibrio bacteriovorus and application thereof
  • Vibrio parahaemolyticus bacteriophage, bdellovibrio bacteriovorus and application thereof
  • Vibrio parahaemolyticus bacteriophage, bdellovibrio bacteriovorus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1 Separation and purification of Vibrio parahaemolyticus phage VP-HYP MCS-1

[0047] 1) The host bacteria are obtained:

[0048] The water samples were collected from the shrimp culture tank with acute hepatopancreatic necrosis and spread on the RO solid plate (yeast powder 1g, peptone 1g, sodium acetate 1g, trace elements 10mL, agar 15g, deionized water 1000mL, pH 7.8 -8.0), cultured at 28°C for 24h. A single colony was randomly picked and streaked for 5 times to purify the single colony. The purified single colony was called MCS-1, which was stored in a -80°C glycerol tube.

[0049] To clarify the taxonomic status of strain MCS-1, the 16S rDNA sequence was amplified using universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'). The sequence amplified by PCR was cloned and sequenced to obtain the full-length 16S rDNA sequence (GenBank accession number MN901166), and the full-length sequence was analyzed and a phylogenetic tree...

Example Embodiment

[0064] Example 2 Separation and purification of Vibrio parahaemolyticus Halobacteriovorax sp.MCS-1

[0065] Qingdao coastal seawater was collected, filtered through a 0.22 μm pore size sterile filter, added to the Vibrio parahaemolyticus MCS-1 bacterial solution in logarithmic growth phase, and cultured in a shaker (28°C, 150rpm). Samples were taken on 1d, 3d, 5d, and 7d, respectively, and the RO liquid medium (1g of yeast powder, 1g of peptone, 1g of sodium acetate, 10mL of trace elements, 100mL of deionized water, pH 7.8-8.0) was used for gradient dilution, and the dilution gradient was 10 -1 , 10 -2 , 10 -3, 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 1 mL of each dilution gradient was mixed with 1 mL of host bacteria, and then placed in a shaker (28°C, 150 rpm) and incubated for 30 min. After the incubation, add 5 mL RO semi-solid medium, mix well and pour it onto each RO solid plate, shake well until the semi-solid solidifies. Cover with parafilm and place in a constan...

Example Embodiment

[0078] Example 3 Host range detection of Vibrio parahaemolyticus phage VP-HYP MCS-1

[0079] The 13 Vibrio strains in the laboratory were selected for the detection of phage host range, among which, 13 Vibrio strains were Vibrio parahaemolyticus (Vibrio parahaemolyticus), Vibrio owensii (Vibrio erwinsii), Vibrio ocaledonicus (New Caledonian Subvibrio), Vibrio plantisponsor (plant probiotic Vibrio), Vibriohanami, Vibrio hyugaensis, Vibrio azureus (Vibrio farthera), Vibrio natriegens (Vibrio natriegens), Vibrio alginolyticus (Vibrio alginolyticus), Vibrio harveyi (Harveyi Vibrio viridis), Vibriovariabilis (Vibrio mutans), Vibrio galatheae, Vibrio gangliei.

[0080] Take 1mL of the above-mentioned different bacteria in the logarithmic growth phase and mix them with 5mL RO semi-solid (about 48°C), and then pour into a double-layer plate, and then drop 10 μL of the above-mentioned embodiment on the solidified RO semi-solid plate to obtain purified. Bacteriophages were cultured in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of microorganism prevention and treatment, and particularly relates to a vibrio parahaemolyticus bacteriophage VP-HYP MCS-1 and bdellovibrio bacteriovorusHalobacterovoraxsp.MCS-1 composition and application thereof to prevention and treatment of prawn pathogenic vibrio parahaemolyticus infection. The vibrio parahaemolyticus bacteriophage is vibrio parahaemolyticus bacteriophage VP-HYPMCS-1, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.199693. The vibrio parahaemolyticus bacteriophage is a vibrio parahaemolyticus bacteriophage VP-HYPMCS-1. The bdellovibrio parahaemolyticus is bdellovibrio parahaemolyticus Halobacterovoraxsp.MCS-1, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.199694. The invention also discloses a preparation method of the bdellovibrio parahaemolyticus strain. The virulent bacteriophage VP-HYP MCS-1 obtained through separation is high in host specificity and has cracking and killing effects on vibrio parahaemolyticus; the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is obtained through separation, the host spectrum of the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is wide, and the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 has cracking and killing effects on various vibrios including vibrio parahaemolyticus; the vibrio parahaemolyticus bacteriophage and bdellovibrio bacteriovorus provided by the invention have good application prospects in prevention and control of prawn pathogenic vibrio parahaemolyticus infection.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to a composition of Vibrio parahaemolyticus phage VP-HYP MCS-1, Bdellovibrio Halobacteriovorax sp. application. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the promotion of intensive farming methods, economic benefits have gradually increased. However, the frequent occurrence of bacterial diseases in aquaculture animals has caused great harm to the aquaculture industry. Among them, Vibrio anguillarum, Vibrio alginolyticus, Vibrio harveyi, Vibrio salmonicida, Vibrio parahaemolyticus and other Vibrio bacteria caused vibriosis is the most serious. [0003] As one of the main aquaculture animals in my country, prawn occupies an important position in the fishery economy. However, the acute hepatopancreas necrosis disease (AHPND) caused by Vibrio parah...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N7/00A61K35/76A61P31/04A61P1/16A61P1/18A01K61/13
CPCC12N7/00A61K35/76A61P31/04A61P1/16A61P1/18A01K61/13C12N2795/00021A61K2300/00Y02A40/81
Inventor 张永雨王增猛孙越超
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap