Novel R. solanacearum bacteriophage and its composition and application

Abstract

The invention relates to the field of microorganisms, and specifically provides Ralstonia solanacearum phage GP1 (Ralstonia solanacearum phage GP1), preservation number CCTCC M 2016633; GP2 (Ralstia solanacearum phage GP2), preservation number CCTCC M 2016634; Ralstonia solanacearum phage GP1; Phage GP3 (Ralstonia solanacearum phage GP3), deposit number CCTCC M 2016635. The advantages of the phage of the present invention include: it is a strict and potent phage and can effectively kill R. solanaceae from different plant host sources, especially R. solanaceae that causes ginger blast; it is non-toxic to normal microbial flora Function; its DNA cannot encode virulence genes; it has strong stability between pH 5 and pH 9; there is no order of magnitude change in potency when stored at 4°C for 1 year; it can effectively inhibit bacteria and kill bacteria at low potency. The bacteriophage of the present invention can prevent and treat crop bacterial wilt caused by R. solanacearum, especially ginger blast, provide excellent strain resources for the development of new bacterial wilt-resistant biological pesticides, and have good application and development prospects.

Description

technical field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to novel R. solanacearum phages, their composition, their preparation method and application. Background technique

[0002] Ralstonia solanacearum is a gram-negative bacterium of the subphylum β-proteobacteria that grows in soil. The pathogen can usually invade the intercellular spaces of the cortex from wounds in the roots or stems of plants or from the root caps of uninjured secondary roots. By destroying the intercellular glue layer, the cell walls are separated, deformed, and cavities are formed, thereby infecting The xylem parenchyma stimulates the small cells near the vessel to form the intrusion and migrate into the intrusion. After the invading body ruptures, R. solanacearum will continue to be released into the duct, and multiply and rapidly spread and expand in the duct, thereby causing the plant to wilt and die (He Liyuan, Kang Yaowei. Pseudomonas sola...

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