The invention discloses a primer group for detecting halophilic
bdellovibrio bacteriovorus based on high-
throughput sequencing and application of the primer group. The primer group comprises primers of which the
nucleotide sequences are as shown in SEQ ID NO.1 and SEQ ID NO.2. The invention further discloses a method for detecting the halophilic
bdellovibrio bacteriovorus based on high-
throughput sequencing. The invention also discloses a halophilic
bdellovibrio detection method based on high-
throughput sequencing. The method comprises the following steps: extracting template
DNA of a sample to be detected; carrying out amplification on the template
DNA by using the two primers; treating an amplification product, constructing a
gene library, and carrying out high-throughput sequencing; after
sequencing data is processed,
statistical analysis is carried out, and the biological diversity of the halophilic bdellovibrio is obtained. According to the primer provided by the invention, diversity detection on the Halobacteriovorax can be realized, the primer has the characteristics of high sensitivity, good
repeatability and the like, construction of a
gene library is completed through one-step amplification, and existence and diversity of the Halobacteriovorax in an environment can be rapidly detected.