107 results about "Bdellovibrio bacteriovorus" patented technology

The invention provides a preparation method and application of a sea bdellovibrio bacteriovorus ecological preparation, and relates to a sea microbial ecological preparation and a preparation method of a bdellovibrio bacteriovorus preparation for mariculture. The preparation method comprises the following steps of: preparing a host bacteria suspension; separating and purifying bdellovibrio bacteriovorus; and propagating and culturing bdellovibrio bacteriovorus. The preparation method is characterized in that after pseudomonas stutzeri is connected with an incline and is activated, adding physiological saline in a test tube to prepare the bacterial suspension; then propagating and culturing with nutrient bouillon; adding a flocculating agent in the obtained bacterial suspension; standing and precipitating after uniformly shaking; adding the physiological saline to obtain 1012 cfu / mL of host bacteria suspension; separating and purifying the bdellovibrio bacteriovorus of collected sea and bottom sediment samples with a double agar plate method; pouring culture media containing the host bacteria suspension and the collected samples into sea agar in the lower layer for culture after uniformly mixing; and adding the host bacteria and the bdellovibrio bacteriovorus obtained by separation into sterilized natural sea, and culturing until the concentration of the bdellovibrio bacteriovorus is 108 to 109 pfu / mL for future use.

The invention relates to an aquaculture composite microbial agent, which comprises the following agents by weight: 5-10 parts of Bacillus subtilis, 0.5-4 parts of Bacillus licheniformis, 1-6 parts of bacillus megaterium, 0.2-1 part of Pseudomonas gelatinasa, 0.5-3 parts of Rhodopseudomonas capsulata, and 2-8 parts of Bdellovibrio bacteriovorus. The invention also relates to application of the aquaculture composite microbial agent in improvement of water quality and / or control of aquatic organism diseases.

The invention discloses a bdellovibrio bacteriovorus microcapsule, the wall of the microcapsule is gelatin, the particle is in a ball shape, the surface has recess without holes and cracks, the particle size of the particle is 3-12 mum, the distribution is basically uniform, wherein the content of bdellovibrio bacteriovorus is 3.69*10<7>pfu / g-3.70*10<7>pfu / g, and the content of escherichia coli DH5 alpha is 8.6*10<9>pfu / g-2.10*10<10>pfu / g. The invention provides a preparation method of the microcapsule, which comprises the following steps: 1) preparing an escherichia coli DH5 alpha bacterium suspension; 2) preparing a bdellovibrio bacteriovorus nutrient solution; and 3)uniformly mixing the escherichia coli DH5 alpha bacterium suspension obtained in the step 1) and the bdellovibrio bacteriovorus nutrient solution obtained in the step 2) with an aseptic gelatin solution, performing spray drying, and finally preparing the microcapsule. The microcapsule obviously increases the anti-infection effect in aquatic product breeding industry.

The invention relates to a method for reducing abundance of multiple antibiotic resistance genes in excess sludge and limiting horizontal transfer of the multiple antibiotic resistance genes. According to the method, parasitic bdellovibrio bacteriovorus can invade other host cells, and polypeptide, amino acid and the like in the host cells are used as energy and a carbon source for self-proliferation; the genetic material in the sludge host cell carrying the antibiotic resistance gene is degraded and reconstructed so as to achieve the purpose of reducing the abundance of a variety of resistance genes, and the degradation of the movable gene element (integron I) further effectively inhibits the horizontal transfer of the antibiotic resistance gene during the biotransformation process. According to the method disclosed by the invention, various resistance genes in the residual sludge can be remarkably reduced, which comprises the abundance of sulfonamides (sul1, sul2), tetracyclines (tetC, tetQ) and class I integron (int1); meanwhile, equipment is simple, energy is saved, secondary pollution is avoided, and an environment-friendly, green, low-carbon, economical and efficient ecological way is provided for relieving antibiotic resistance gene pollution of municipal excess sludge, developing series sludge recycling products such as landscaping matrix soil and soil conditioners, widening the land utilization range and widening the utilization amount of the recycling products.

The invention discloses a bioremediation nutritional agent for a water balance system and a preparing method thereof. The bioremediation nutritional agent comprises an algae component, a water-aqua microorganism component and a nutrient granule and powder component. The algae component comprises chlorella and plant cytokinin added to the chlorella; the water-aqua microorganism component comprises photosynthetic bacteria, saccharomycetes, lactic acid bacteria, composite bacilli, streptococcus faecalis, clostridium butyricum, bdellovibrio bacteriovorus and ferment; the nutrient granule and powder component comprises phosphorus pentoxide, rare earth, silicon earth, organic fermentation substances and a potentiating agent. The preparing method includes the steps of preparing the algae component, preparing the water-aqua microorganism component and preparing the nutrient granule and powder component. According to the bioremediation nutritional agent and the preparing method thereof, due to the theory of the yin and yang balance rule, under the effect of beneficial monodus subterraneus and beneficial microorganisms, rotten water plants are effectively decomposed, the nitrogen-and-phosphorus-balanced conversion function is achieved, dissolved oxygen of a water body is increased, the metabolism immunologic function of water products is improved, breeding cost is greatly reduced, and the ecological circulation water environment is formed.