Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Acinetobacter baumannii bacteriophage and medical application

A technology of Acinetobacter baumannii and phage, which is applied in the field of bioengineering and can solve the problems of few studies on Acinetobacter baumannii phage

Pending Publication Date: 2020-02-04
JILIN UNIV FIRST HOSPITAL
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few domestic studies on Acinetobacter baumannii phages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Acinetobacter baumannii bacteriophage and medical application
  • Acinetobacter baumannii bacteriophage and medical application
  • Acinetobacter baumannii bacteriophage and medical application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Phage isolation and preparation

[0016] The isolation procedure of phage is described in detail below. The sewage sample in the present invention is collected from Huoshao Li in Changchun City, and the host bacteria is Acinetobacter baumannii ABC3. Collect sewage, filter it with gauze, and replace ddH with treated sewage 2 O Prepare LB medium (100mL); add 1 mL of overnight cultured host bacteria ABC3 to the medium, and incubate at 37°C for 10-12 hours; take 1 mL of the culture, centrifuge at 12000r / min for 2 min, and filter the supernatant with a 0.22 μm filter to form phage The stock solution was kept and the resulting filtrate was used for plaque testing to check for the inclusion of phage capable of Acinetobacter baumannii ABC3.

[0017] Plaque test: inoculate Acinetobacter baumannii ABC3 in 5ml LB liquid medium at a ratio of 1%, and cultivate overnight at 37°C with shaking. Take 100 μL of the bacterial culture solution prepared above and drop it on the LB plate,...

Embodiment 2

[0020] Phage amplification and purification

[0021] On the double-layer plate forming phage plaques, use the tip of a sterile pipette to pick up a single phage plaque with a large diameter and translucency, inoculate it in 5 ml LB liquid medium, add 200 ml of phage host bacterial solution μL, shake culture at 37°C for 5 h, centrifuge at 12,000 r / min, 4°C for 10 min, and take the supernatant; repeat the double-layer plate experiment, and repeat this 4-5 times until the phages form plaques of the same size.

[0022] Take 1 mL of freshly cultured host bacteria and add 300 μL of phage lysate (the ratio of a single phage culture to host bacteria is 1:1, 1:10 and 1:100, respectively). Incubate at 37°C for 20 minutes to make the phage particles adsorb to the host bacteria; add 800mL TSB liquid medium, then add CaCl 2 The mother liquor was grown to a final concentration of 1.25mM, shaken at 37°C for 6-8h, centrifuged at 12000 r / min, 4°C for 10min, and the supernatant was taken, whi...

Embodiment 3

[0029] Transmission electron microscope observation of bacteriophage VB_AbaM_DC31

[0030] Take the PEG-purified phage in Example 2 for electron microscope observation. The specific operation steps are: add 10 μL of the sample and drop it on the copper grid, wait for it to settle for 15 min, absorb the excess liquid with filter paper, and use 2% phosphotungstic acid (PTA ) stained for 1-2min, and observed with a transmission electron microscope (Hitachi H-7650) after drying; the observation results are as follows figure 2 As shown, the head is in the shape of an icosahedron, the diameter of the head is about 35-40 nm, and the length of the tail is about 80-90 nm. According to the "Classification of Viruses—The Eighth Report of the International Committee on Taxonomy of Viruses" published by the International Committee on Taxonomy of Viruses (ICTV) in 2005, VB_AbaM_DC3 belongs to Myoviridae.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Incubation periodaaaaaaaaaa
Login to View More

Abstract

The invention discloses a strain of acinetobacter baumannii bacteriophage. The acinetobacter baumannii bacteriophage is a novel bacteriophage with cytotoxicity, deposited in the China Center for TypeCulture Collection on September 9, 2019 and named as acinetobacter baumannii bacteriophage VB_AbaM_DC3 with preservation number of CCTCC NO: M 2019705. The invention further provides a medical application of the acinetobacter baumannii bacteriophage. The bacteriophage has a wider lysis spectrum and can lyse 10 different clinical isolates of acinetobacter baumannii. The bacteriophage can be used alone or in combination with acinetobacter baumannii bacteriophages other than the bacteriophage of the present invention or other disinfecting and sterilizing substances to reduce numbers of the acinetobacter baumannii in intensive care units (ICU) and general wards in hospitals, thereby reducing incidence of nosocomial infection.

Description

technical field [0001] The invention discloses an Acinetobacter baumannii phage, which is a novel phage with killing activity; meanwhile, it also provides the medical application of the phage, and the invention belongs to the field of bioengineering. Background technique [0002] Acinetobacter baumannii is an important pathogen, which is widely distributed in humid environments and can attach to the body surface of humans and animals. Acinetobacter baumannii mainly causes respiratory tract infection, and can also cause various diseases such as sepsis, urinary tract infection, and secondary meningitis. In addition, Acinetobacter baumannii is easy to form a biofilm, which can be adsorbed on the hands of medical staff or medical equipment, causing the occurrence and spread of infection. In addition, due to the indiscriminate use and abuse of antibiotics, the drug resistance of Acinetobacter baumannii has become increasingly serious, leading to the emergence of "super bacteria"...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCC12N7/00A61K35/76A61P31/04C12N2795/00021
Inventor 郭志敏顾敬敏韩文瑜冀亚路程梦珺黄晶
Owner JILIN UNIV FIRST HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com