48 results about "Salmonella stanley" patented technology

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The invention discloses enterococcus faecium capable of degrading three mycotoxins at the same time and application of the enterococcus faecium, and belongs to the technical field of microorganisms. The enterococcus faecium JBHBIO-BSC1 disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the enterococcus faecium JBHBIO-BSC1 is CCTCC (China Center for Type Culture Collection) NO: M 20241918. The enterococcus faecium disclosed by the invention has the most remarkable characteristic that the enterococcus faecium has an obvious degradation effect on the aflatoxin, the vomitoxin and the zearalenone, and the degradation rates of the aflatoxin, the vomitoxin and the zearalenone can respectively reach 74.67%, 85.83% and 82.12%. The enterococcus faecium disclosed by the invention is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, and has relatively strong organic acid production capacity. In addition, the strain has an obvious inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and is expected to be applied to the fields of food processing and microbial pharmacy.

The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a (CRISPR / Cas12a) micro-fluidic chip aptamer sensor based on G4 dimer enhancement and application of the CRISPR / Cas12a The sensor adopts a three-layer PMMA (Polymethyl Methacrylate) structure and integrates a replaceable self-assembled pre-stored reagent tube and a contraction-expansion micro-mixing channel with a rhombic micro-column, so that the rapid, sensitive and specific detection of salmonella typhimurium is realized; wherein the rhombic micro-column design of the micro-mixing channel can generate asymmetric vortexes, so that the fluid mixing efficiency is enhanced; the self-assembly pre-storage reagent tube realizes automatic addition of the reagent, so that the chip can be repeatedly used. The detection range of the sensor is 3.6 * 10 < 1 >-3.6 * 10 < 6 > CFU / mL, the detection limit is 36 CFU / mL, and the recovery rate of the sensor in actual samples such as milk, pre-cut vegetables and chicken is 88.11-105.19%. According to the present invention, the detection sensitivity is improved through the fluorescence enhancement effect of the G4 dimer, the automation and the high efficiency of the detection process are achieved by combining the microfluidic technology, and the method is suitable for the food safety detection field.

This invention provides a method for monitoring and evaluating the risk of pathogenic bacteria transmission in poultry farm feces, belonging to the field of livestock and poultry biosecurity control technology. The method includes evaluating the risk of Salmonella transmission during the poultry physiological cycle, assessing the risk of antibiotic resistance associated with drug administration behavior, and monitoring the function during composting based on microbial community structure and metabolic function. This invention can accurately locate the highest-risk nodes of pathogen contamination and antibiotic resistance evolution during the breeding cycle, solving the problem of inaccurate identification of risk sources in existing technologies and avoiding poultry product contamination due to missed risk assessments. It overcomes the single-dimensional limitation of existing technologies that only detect the quantity of pathogens, comprehensively evaluating pathogen risk from phenotypic, molecular, and functional levels. The assessment results are more in line with the actual biosecurity needs of poultry farming, ultimately reducing the safety risks of poultry product contamination and the spread of antibiotic resistance.

A vaccine comprising effective amounts of i) E. coli colonization factor antigens comprising CFA / I, CS3, CS5 and CS6 antigens; ii) a heat labile enterotoxin B subunit antigen being an LCTBA protein; and iii) an adjuvant being double mutant (R192G / L211A) of E. coli heat-labile toxin (dmLT), for use in inducing protective immunity against diarrheal disease caused by Salmonella spp. and / or Shigella spp.

The application discloses a preparation method of a recombinant ternary fusion protein dressing with epidermal cell activation function. The dressing is a fusion protein designed based on the research on the functions and properties of COL3A1 and IFN-kappa proteins by international peers and the laboratory. The specific method is that the fusion gene fragment of the covalent connection of COL3A1 and IFN-kappa with GGSGG as a linker is obtained by using overlap extension PCR technology, and a 6×His label is added. The target gene is cloned into a prokaryotic expression vector pET30 (modified in the laboratory) to obtain the fusion protein COL3A1-IFN-kappa with an MBP label. After expansion culture, the MBP-IFN-kappa-COL3A1 with a purity of more than 90% can be obtained through Ni-NTA column affinity chromatography. We verified the resistance of the fusion protein to salmonella in THP-1 cells, and verified the effect of the fusion protein on promoting wound healing and resisting salmonella infection on mice. Finally, it is proved that the MBP-IFN-kappa-COL3A1 has good uniformity, is easy to purify, has high activity, has no side effects, and is very suitable for being used as an epidermal cell activation dressing.

The invention discloses a campylobacter jejuni rapid detection method and a kit based on a specific molecular target rpoZ. The sequence of the molecular target is as shown in SEQ ID NO. 1. The PCR / qPCR detection kit for detecting campylobacter jejuni is established on the basis of the target rpoZ, has extremely high specificity, only has specific amplification signals for nucleic acid of campylobacter jejuni, and has no cross reaction for other common food-borne pathogenic bacteria such as escherichia coli, salmonella and staphylococcus aureus. Meanwhile, the sensitivity is high, and the nucleic acid detection target can detect the campylobacter jejuni genome DNA as low as 132fg / mu L. The method can effectively detect campylobacter jejuni in samples such as food, is high in specificity, good in accuracy and simple to operate, and can be applied to the fields of food safety and the like.

The invention discloses a compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method of the compound preparation. The compound preparation is prepared from the following components in parts by weight: 3.2 to 5.8 parts of astragalus polysaccharide powder, 2.9 to 5.3 parts of xylooligosaccharide powder, 2.5 to 4.8 parts of compound enzyme agent, 2 to 4.4 parts of compound bacterium agent, 1.5 to 3.6 parts of selenium yeast powder, 1.3 to 3.2 parts of egg yolk antibody, 1 to 2.1 parts of hydroxypropyl methyl cellulose, 0.35 to 0.8 part of chitosan and 0.3 to 0.65 part of montmorillonite. The compound preparation disclosed by the invention can effectively inhibit growth and reproduction of viruses such as riemerella anatipestifer, escherichia coli and salmonella, and is beneficial to regulation of immune functions of animals and improvement of body resistance of the animals.

The application discloses a bacteriostatic type of liquor dreg containing Zhangzhou Bacillus GBW-NX and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou Bacillus GBW-NX is CGMCC No. 27402. It is proved through experiments that the Zhangzhou Bacillus GBW-NX has the bacteriostatic effect on inhibiting Escherichia coli, Salmonella and Staphylococcus aureus. The Zhangzhou Bacillus GBW-NX is placed in a solid culture medium containing the liquor dreg to be fermented and enzymolyzed to prepare the bacteriostatic type of liquor dreg. The bacteriostatic substance of the bacteriostatic type of liquor dreg is a bacteriocin. It is also proved that the bacteriostatic type of liquor dreg can improve the intestinal function of ruminants including lactating cows, and can also improve the milk yield of the cows, thereby widening the application range of the Zhangzhou Bacillus GBW-NX, providing a new direction for the feeding of ruminants, and having important significance for the breeding of ruminants.

The invention discloses colloidal gold immunochromatography test paper for rapidly detecting pigeon I-type paramyxovirus and a preparation method of the colloidal gold immunochromatography test paper. Viral diseases of pigeons, especially pigeon plague virus and pigeon adenovirus, are rapidly detected on the basis of a newly developed test strip, and the operation is simple and convenient; according to the design of antigens and antibodies of the pigeon plague virus and the pigeon adenovirus, rapid detection of the pigeon plague virus and the pigeon adenovirus can be conveniently carried out, and the obtained test strip is high in specificity and high in sensitivity. The pigeon I-type paramyxovirus detection kit has the advantages that the pigeon I-type paramyxovirus detection kit does not have cross reaction with chicken infectious bronchitis, chicken egg drop syndrome, chicken Marek's disease, avian influenza, fowl adenovirus, infectious bursal disease, escherichia coli, salmonella and other samples, but can have cross reaction with Newcastle disease, so that the pigeon I-type paramyxovirus detection kit can be used for diagnosing pigeon I-type paramyxovirus and Newcastle disease.

The invention discloses a novel Kdo-MPLA adjuvant, and relates to the technical field of synthetic biology, the adjuvant is formed by connecting monophosphate lipid A (MPLA) and Kdo sugar through an alpha-glucosidic bond, MPLA is of a structure formed by modifying salmonella lipid A through 4 '-site monophosphorylation, and the Kdo sugar is endowed with amphipathy, so that the Kdo sugar is self-assembled into liposome nanoparticles in an aqueous solution; the rfaC and eptA genes are double knocked out through a gene editing salmonella attenuated strain, wherein the rfaC knockout blocks the synthesis of LPS core polysaccharide, and lipid A-Kdo connection is reserved; knocking out eptA to obtain a monophosphorylated lipid A; after the engineering bacteria are fermented, high-purity Kdo-MPLA is obtained by adopting a phenol-chloroform-petroleum ether extraction method, the process is simplified, and the product is uniform; the adjuvant can activate a TLR4 signal channel and induce Th1 type immune response, the levels of inflammatory factors IL-6 and IL-1beta are remarkably lower than those of natural LPS, and the adjuvant is suitable for a mixed preparation of vaccine antigens such as HPV and malaria; the method overcomes the defects of hydrolysis uncontrollability and high chemical synthesis cost of the traditional MPLA chemical extraction process.

This application provides a perilla protein polypeptide obtained by enzymatic hydrolysis of perilla protein using ficin. Prepared from the safe food ingredient perilla, the perilla protein polypeptide exhibits significant antibacterial effects against a variety of Gram-negative and Gram-positive avian pathogens, including Enterococcus gallinarum, Staphylococcus aureus, and Salmonella gallinarum, providing a new treatment option for infectious diseases in poultry.

The invention discloses a rabbit source fermentation lactobacillus mucus RLF77 and an application of the rabbit source fermentation lactobacillus mucus RLF77. The strain is separated from intestinal tracts of weaned young rabbits and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252224. The bacterial strain has the excellent characteristics of being used as a probiotic, being resistant to gastrointestinal tract environment and high in intestinal epithelial cell adhesion rate, and being capable of effectively inhibiting various pathogenic bacteria such as escherichia coli, salmonella and the like. Animal experiments show that the production performance of weaned young rabbits can be remarkably improved, the diarrhea rate can be reduced, the survival rate and the oxidation resistance can be improved by feeding the rabbit-derived RLF77 strain, and the effect is superior to that of non-rabbit-derived probiotics. The invention provides a new strain resource for developing green breeding products for replacing antibiotics.

The invention provides an antibacterial peptide ATMP23 mutant with improved antibacterial activity and application of the antibacterial peptide ATMP23 mutant in preparation of an antibacterial agent. The amino acid sequence of the antibacterial peptide ATMP23 provided by the invention is shown as SEQ ID NO: 1, amino acid mutation and screening are carried out on the basis of the amino acid sequence, and antibacterial peptide mutants A23-M4, A23-M5 and A23-M6 with significantly improved antibacterial activity are obtained. The antibacterial peptide ATMP23 mutant provided by the invention is small in molecular weight, convenient to artificially synthesize and low in cost, and has a good bactericidal effect on clostridium welchii, staphylococcus aureus, streptococcus, vibrio parahaemolyticus, vibrio alginolyticus, vibrio splendidus, salmonella, escherichia coli and the like; when being applied to livestock and aquatic feed, the feed conversion ratio of livestock and aquatic products can be remarkably reduced, the growth performance and immune function indexes of bred animals can be improved, and the feed additive can be widely applied to the industries of feed, food, medicine and the like.

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

The invention provides a salmonella drug resistance monitoring data security management system based on a block chain, and belongs to the technical field of data transmission management. A hierarchical encryption mechanism is constructed, the technical characteristics of the block chain are fused, a standardized feature combination template architecture is designed, and a matched hierarchical transmission and intelligent contract verification system is used; a differential encryption strategy is implemented on the basis of data sensitivity, and the security of each level of data is guaranteed in combination with symmetric and asymmetric encryption technologies; through characteristic parameter modularization recombination and template association identification, data integrity is guaranteed, transmission efficiency is optimized at the same time, and scattered data can be precisely restored into a structured data set; and finally, through consensus node verification driven by an intelligent contract and on-chain state updating, whole-process standardized management and control of data from collection, transmission to storage are realized, and a salmonella drug resistance monitoring data security management mode with security, efficiency and traceability is constructed.

The present disclosure relates to novel immunogenic monovalent and multivalent polysaccharide-protein conjugate vaccine compositions comprising a polysaccharide selected from Salmonella serovar strains S. typhi; S. paratyphi A; S. typhimurium and S. enteritidis and alternative improved methods of polysaccharide fermentation, polysaccharide purification, polysaccharide-protein conjugation and stable formulation. The present disclosure further relates to methods for inducing an immune response in subjects against Salmonella and non-typhi related diseases and / or for reducing or preventing Salmonella typhi and non-typhi related diseases in subjects using the compositions disclosed herein. The vaccine elicits bactericidal antibodies and is useful for prevention of gastroenteritis, enteric and typhoid fever.

The invention establishes a method for simultaneously detecting vibrio parahaemolyticus, staphylococcus aureus, listeria monocytogenes, salmonella, shigella, bacillus cereus and vibrio alginolyticus on the basis of multiple PCR (Polymerase Chain Reaction) combined with a gene chip technology. The method comprises the following steps: selecting a heat-labile hemolysin gene of vibrio parahaemolyticus, a heat-resistant nuclease gene of staphylococcus aureus, a coded hemolysin O gene hlyA of listeria monocytogenes, an invasive protein A gene of salmonella enteritidis and an invasive plasmid antigen H gene of Shigella to carry out quintuple PCR (Polymerase Chain Reaction) amplification; bacillus cereus non-hemolytic toxin genes and vibrio alginolyticus similar Rpos sigma factor genes are selected for double PCR, two groups of multiplex PCR amplification products are hybridized with specific probes on the chip at the same time, and seven food-borne pathogenic bacteria can be detected at the same time. The method for detecting pathogenic bacteria by using the gene chip is high in specificity, high in sensitivity and strong in reliability.

This invention discloses a multilayer microsphere for the prevention and / or treatment of bacterial diarrhea, its preparation method, and its applications, relating to the field of biomedical technology. The multilayer microsphere has a three-layer core-shell structure: the core layer is loaded with *Lactobacillus plantarum* and sodium butyrate, the middle layer is a chitosan coating layer, and the outer layer is a sodium alginate coating layer. This invention has found that *Lactobacillus plantarum* and sodium butyrate have a significant synergistic antibacterial effect, effectively inhibiting the proliferation of pathogenic *Escherichia coli* and *Salmonella*. In vivo experiments have confirmed that the multilayer microsphere can effectively restore the body weight of diarrhea-prone animals, repair pathological damage to the jejunum and colon, and alleviate intestinal inflammation at the molecular level by inhibiting the activation of NF-κB and MAPK inflammatory pathways, downregulating the expression of pro-inflammatory factors IL-1β, IL-6, and TNF-α, and upregulating the level of the anti-inflammatory factor IL-10. The multilayer microsphere provided by this invention can serve as a novel, green, and safe formulation suitable for the prevention and control of bacterial diarrhea in livestock and poultry farming.

The invention discloses an immortalized goat bone marrow-derived macrophage for expressing an SV40LT gene and application of the immortalized goat bone marrow-derived macrophage, and belongs to the technical field of bioengineering. The immortalized goat bone marrow-derived macrophage provided by the invention is obtained by knocking an immortalized gene SV40LT into a primary goat bone marrow-derived macrophage ROSA26 site through a CRISPR-Cas 9 system, and the immortalized goat macrophage provided by the invention is an immortalized goat macrophage cell strain which is suitable for being infected with salmonella and brucella and can be continuously and stably passaged; the method can be effectively applied to immunological research on goat brucella infection resistance and production of brucella vaccines.

A genetically modified Salmonella cell (GMSC) engineered to display pattern-associated and danger-associated molecular patterns to recruit and enhance innate immunity and exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and / or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

The invention discloses a composition and method for detecting food-borne pathogenic bacteria and application, and belongs to the technical field of food safety detection. The gDNAs and fluorescent probe combination suitable for multi-target parallel detection is obtained through multiple rounds of screening, meanwhile, the advantages of LAMP efficient amplification and PfAgo targeted cutting are combined, a multiple LAMP-PfAgo rapid detection method for three pathogenic bacteria of staphylococcus aureus, salmonella and listeria monocytogenes is established, and the problem that LAMP multiple detection is difficult is effectively solved. According to the present invention, the specificity is high, the result determination is simple, the detection limit on the pure culture can achieve 101 CFU / mL, the detection can be completed within 45 min, and the important practical application value is provided.

The present invention discloses a monoclonal antibody CJ116 against pathogenic Campylobacter and its application. In the monoclonal antibody CJ116, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of the light chain variable region is as shown in SEQ ID NO.5. This monoclonal antibody CJ116 has high affinity and high specificity, and the detection sensitivity for Campylobacter jejuni and Campylobacter coli can reach 10<supgt;2< / supgt> CFU / mL, and the detection sensitivity for Campylobacter fetus, Campylobacter upsaliensis and Campylobacter lari is 10<supgt;3< / supgt> CFU / mL, but there is no cross-reaction with other common intestinal pathogenic bacteria such as pathogenic Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica, Shigella flexneri, Shigella boydii and Staphylococcus aureus.

The invention belongs to the technical field of microorganisms, and particularly relates to lactic acid bacteria and application thereof. The bacterial strain is Ligilobacillus sativus SunLab-GHL01 (L.sativus GHL01 for short), is separated from lungs of high-production-performance Ailijiai broiler chickens and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the bacterial strain is CCTCC NO: M 20251725. The invention further discloses a preparation method of the high-production-performance Ailijiai broiler chicken with high-production-performance Ailijiai broiler chicken with high-production-performance Ailijiai broiler chicken with high-production-performance Ailijiai broiler chicken. Through morphology, physiology and biochemistry, 16S rRNA sequence identification and whole genome sequencing analysis, the taxonomic status and functional characteristics of the 16S rRNA are defined. The strain has good acid resistance, cholate resistance and acid production capacity, has broad-spectrum antibacterial activity, and can effectively inhibit pathogenic bacteria such as escherichia coli, pathogenic escherichia coli, salmonella, staphylococcus aureus and pseudomonas aeruginosa. Animal experiments show that the poultry feed conversion rate and the anti-stress capability can be remarkably improved by atomizing the strain. The strain and the application thereof can be used for improving the health condition and the production performance of poultry under the intensive breeding condition, and have application prospects in the fields of feed additives, probiotic preparations, animal health products and the like.

The invention relates to the technical field of food safety, and discloses a food microbial contamination rapid detection method fusing a knowledge graph and deep learning, and the method comprises a data collection module, a knowledge graph engine, a deep learning calculation unit, a dynamic optimization module, a visual interaction terminal and a traceability management platform. A micro-fluidic chip and impedance sensing combined technology is adopted, the traditional long-time detection period is shortened, the real-time monitoring requirement of cold-chain logistics is met, a spectrum-bioelectricity-microscopic image three-mode space-time alignment model is created for the first time, dimension gaps are eliminated through feature space mapping, the recognition accuracy of complex matrix pollution is improved, and the real-time monitoring requirement of the cold-chain logistics is met. By constructing a rule engine driven by an industry knowledge graph, dynamic model updating is achieved through an incremental learning module, the detection rate of novel salmonella variants is increased, and the problem of the omission ratio of new pathogens is solved.

The utility model discloses a method for using nano-enhanced loop-mediated isothermal amplification-recombinase polymerase amplification (LAMP-RPA) biosensor kit for rapid cage-side detection of Salmonella species in poultry farms. The method involves processing fecal sample collected from chickens, rehydrating the assay components with Silver nano particles (AgNPs) solution, activating the assay with Magnesium acetate (Mg(OAc)2). Interpreting results is achieved by a naked eye observation of change in color of the mixture. A positive result is indicated by the retention of the original dark gray to dark violet color of the mixture, otherwise, it is signaled by the appearance of a colorless to yellow mixture. This was done in a single-tube reaction, enabling a rapid, equipment-free, and userfriendly nucleic acid-based diagnostic platform ideal for on-site deployment. Diagnostic validation of specificity of the biosensor kit exhibited good results. Moreover, it can detect down to 64 picogram of DNA per reaction or a 10?³ dilution.

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp. / Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1 / Shiga toxin A, or Escherichia coli stx2 / Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

This invention discloses a sodium caseinate-based Pickering emulsion, its preparation method, and its application, belonging to the field of vaccine adjuvant technology. This invention is the first to propose using Salmonella Typhimurium flagellin and sodium caseinate as raw materials to formulate a Pickering emulsion. The prepared Pickering emulsion has the characteristics of small particle size, good biocompatibility, and high stability, and can induce a high immune response in the body. This invention is also the first to propose the application of the Pickering emulsion formulated using Salmonella Typhimurium flagellin and sodium caseinate as raw materials as a vaccine adjuvant. The Pickering emulsion prepared by this invention has a simple preparation process, requiring only mixing and homogenization, and can effectively load the model antigen OVA. This emulsion has good cellular safety and can promote the endocytosis of exogenous antigens by macrophages. The emulsion can induce the body to produce high levels of antibody titers, with antibody titers reaching more than 120,000 times.

The invention provides a primer group and a kit for detecting four bacterial abortion diseases of sheep, belongs to the technical field of biological detection, establishes a multiplex PCR (polymerase chain reaction) detection method for sheep brucellosis, sheep abortion salmonellosis, listeriosis and chlamydia disease and a kit thereof, and has the characteristics of high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like. The rapid differential diagnosis of four sheep bacterial abortion diseases can be realized through one-time PCR amplification, and the method has important advantages in the field of sheep disease diagnosis and identification.