130 results about "Salmonella stanley" patented technology

Attenuated mutant Salmonella bacteria containing inactivated virulence genes are provided for use in safe, efficacious vaccines.

The present invention provides a vaccine, method of use, and kit employing genetically isolated and stabilized, live attenuated bacterial strains including Salmonella that express one or more avian influenza antigens for use in live vaccine compositions that can be orally administered to an individual to protect against avian influenza. Genetic stabilization may be achieved through deletion of IS200 elements and bacteria phage and prophage elements. The bacterial strains may be genetically isolated from external phage infection by constitutive expression of a P22 phage repressor. Nucleic acid sequences encoding antigenic hemagglutinin and neuraminidase avian influenza proteins, having at least one modified codon for optimum expression when transferred into a prokaryotic microorganism for improved immunogenicity

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention provides a method used for simultaneous detection of three food-borne pathogenic bacteria based on multicolor upconversion fluorescence labeling. According to the method, three upconversion materials with differentiable fluorescence spectrums are used for forming multicolor upconversion fluorescent nanoprobes via respective connection with aptamers of staphylococcus aureus, vibrio parahaemolyticus, and salmonella, and complementary oligonucleotide single chains of the aptamers are connected with magnetic nanoparticles so as to form nano-composites. When bacteria to be tested are in a detection system, double chain unwinding is realized because of specific binding of the pathogenic bacteria with corresponding aptamers; it is possible to realize simultaneous quantitative determination of staphylococcus aureus, vibrio parahaemolyticus, and salmonella by monitoring upconversion fluorescence signal strength at 477nm, 550nm, and 660nm, detection linear range ranges from 50 to 1000000cfu / ml, and detection limits are 25cfu / ml, 10cfu / ml, and 15cfu / ml respectively. The method is used for detection of pathogenic bacteria, is high in sensitivity, is rapid and convenient, and can be used for detection of the three pathogenic bacteria in food such as milk and shrimp meat; and results are accurate and reliable.

The invention discloses a quadruple fluorescent PCR (Polymerase Chain Reaction) detection kit for common chicken food-borne bacteria and an application method thereof. The kit contains a fluorescent PCR solution and standards of Clostridium perfringens, Salmonella enteritidis, Escherichia coli O157: H7 and Campylobacter jejuni, wherein the fluorescent PCR solution contains Taq DNA (deoxyribonucleic acid) polymerase, a PCR buffer solution, Mg<2+>, dNTPs (deoxy-ribonucleoside triphosphates), four pairs of bacterial specific primers and four kinds of corresponding fluorescent probes. According to the detection kit and the application method thereof, four kinds of chicken-carried main food-borne pathogenic bacteria, namely Clostridium perfringens, Salmonella enteritidis, Escherichia coli O157: H7 and Campylobacter jejuni, can be rapidly and accurately detected by using quadruple fluorescent PCR, and the detection kit can be applied to the assay of bacteria, the diagnosis of diseases and epidemiological investigation.

The invention relates to the field of food safety, and particularly discloses a broad-spectrum salmonella phage LPSTLL and application. The preservation number of the salmonella phage LPSTLL is CCTCCNO: M 2019049. The salmonella phage LPSTLL is a virulent phage, has a very broad host spectrum and can lyse 13 kinds of serotype salmonellae such as salmonella typhimurium, salmonella enteritidis andsalmonella dublin and lyse a plurality of strains of drug-resistant salmonellae. The invention further discloses bacteriostatic application of the phage as a biological control agent in food storage.The phage particularly can inhibit contamination caused by the proliferation of salmonellae in milk and chicken, and therefore the safety and hygiene of food are ensured.

The present invention provides a novel class of monoclonal antibodies which have a high affinity, broad spectrum neutralizing reactivity to flagellin from various Gram-negative bacteria including, but not limited to, E. coli, Salmonella, Serratia, Proteus, Enterobacter, Citrobacter, Campylobacter and Pseudomonas. The present invention further provides methods of treating infections and diseases using anti-flagellin antibodies in humans, other animals and birds.

The invention relates to a compound gene chip for detecting salmonella serotype and a detection method thereof. salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella paratyphi C, salmonella typhisuis, salmonella typhi and salmonella enterica can be identified by integrating probes for detecting salmonella serotype target genes viaB, tyv, filiC-alpha, fliCl, rfbJ, fliC2, invA and hilA on the same chip. The invention can rapidly and accurately carry out the preliminary screening and identification of salmonella and serotype thereof, greatly shortens detection time and is applicable to the requirement of high throughput detection.

The invention belongs to the application field of preparation technologies of microorganism additives for livestock, and particularly relates to the selective breeding and the application of bacillus subtilis for alleviating the stress of livestock and poultry. The Bacillus subtilis (Bacillus subtilis) HDRaBS1 which has an obvious bacteriostasis effect on common intestinal tract pathogenic bacteria of breeding animals, such as staphylococcus aureus, escherichia coli and salmonella, is separated and screened from a poultry source, the bacterial strain is preserved in the China Center For Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2011034. The verification of biological functions indicates that the bacterial strain has the characteristics of high stress resistance, stress resistance and the like, the bacterial strain can be used for preparing the microorganism preparation for resisting the stress of the livestock and the poultry, and the preparation comprises the microorganism additives for feed of the livestock and the poultry. The invention further discloses a separation and identification method of the bacillus subtilis HDRaBS1 and the application of the staphylococcus aureus for preparing single microbial inoculum for the livestock and the poultry.

The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and / or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.

The invention relates to the technical field of biological detection, and particularly discloses a multiplex-PCR (Polymerase Chain Reaction) detection primer group and kit for various duck-derived pathogenic bacteria. The primer group comprises four pairs of primers which are RArpoB-P1 and RArpoB-P2, E.coliphoA-P1 and E.coliphoA-P2, SalminvA-P1 and SalminvA-P2, Strep16srRNA-P1 and Strep16srRNA-P2. The four pairs of primers are used for carrying out multiplex-PCR amplification and can be used for simultaneously, rapidly, conveniently, specifically and sensitively detecting four bacteria which are riemerella anatipestifer, escherichia coli, salmonella and streptococcus. The four pairs of primers can be used for identification of bacteria, disease diagnosis and epidemiological investigation and has broad market prospect and high economic benefits.

The invention discloses an anti-prawn white spot syndrome virus (WSSV) recombinant Salmonella typhimurium strain, and a preparation method and an application thereof. The preparation method comprises the steps of: extracting WSSV genomic DNA from an infected prawn, and obtaining WSSV envelope protein VP28 genes by a PCR method; extracting total RNA from a silkworm fat body, and obtaining silkworm antimicrobial peptide CD genes by a reverse transcription PCR method; constructing an eukaryotic expression plasmid pcDNA3.1(-)-VP28-CD containing the WSSV envelope protein VP28 genes and the silkworm antimicrobial peptide CD genes; and transforming into attenuated Salmonella typhimurium W0420 strain competent cells. The WSSV recombinant Salmonella typhimurium strain can be used for preparing a vaccine, which can be directly orally fed prawns. Compared with the current commonly used protein vaccines and injectable DNA vaccines, the inventive vaccine has double functions of inhibiting WSSV infection and enhancing prawn immunity.

The invention provides a primer and a probe sequence for multiple fluorescence PCR synchronous detection of nucleotide segments of three types of malignant bacteria of salmonella, vibrio parahaemolyticus and bacillus coli O157:H7. The primer and the sequence are as shown in figures.

The present invention provides a novel class of monoclonal antibodies which have a high affinity, broad spectrum neutralizing reactivity to flagellin from various Gram-negative bacteria including, but not limited to, E. coli, Salmonella, Serratia, Proteus, Enterobacter, Citrobacter, Campylobacter and Pseudomonas. The present invention further provides methods of treating inflammatory bowel disease (IBD) and methods of treating enterobacterial infections using anti-flagellin antibodies in humans, other animals and birds.

The invention discloses m-PCR (Multiplex Polymerase Chain Reaction) primers, probes and detection methods for vibrio cholerae, vibrio parahaemolyticus and salmonella. The primers and the probes are detection primer and probe for fimY genes of the salmonella with sequences shown as SEQ ID NO. 1 to 3 (Sequence Identity Numbers 1 to 3), detection primer and probe for hlyA genes of the vibrio cholerae with sequences shown as SEQ ID NO. 4 to 6 and detection primer and probe for toxR genes of the vibrio parahaemolyticus with sequences shown as SEQ ID NO. 7 to 9 respectively. On the basis, the invention also discloses a detection method containing the primer and probe sequences. The probes and the primers have high specificity; and detection kits and the method are simple and convenient, are easy to use, and have accurate results and extremely high specificity and sensitivity.

The invention relates to a multiple PCR identification method of salmonella serogroups A, B, C1, C2 or D, belonging to the technical field of food safety detection. The method comprises the following steps: step 1: respectively designing specific amplification primer pairs according to a base sequence such as 5 nucleic acids as shown in SEQ ID NO:1-5; and step 2: detecting a sample by utilizing the specific amplification primer pairs obtained in the step 1 and adopting a conventional PCR method, and determining the type of the salmonella serogroup in the sample. The determined type of the salmonella serogroup in the sample is concretely as follows: the gel electrophoresis detection is carried out, wherein if 350bp and 466bp bands exist, the result shows that the serogroup A exists; if 177bp bands exist, the result shows that the serogroup B exists; if 623bp bands exist, the result shows that the serogroup C1 exists; if 540bp bands exist, the result shows that the serogroup C2 exists; and if 466bp bands exist, the result shows that the serogroup D exists. The method of the invention can be used for quickly and accurately identifying the salmonella of the serogroups A, B, C1, C2 or D, and avoids the defects of complicated operation, time and labor consumption and high cost of the existing method.

The invention discloses an LAMP primer combination for detecting 8 environmental pathogens of dairy cow mastitis and application thereof. The primer combination provided by the invention is composed of 48 primers shown in sequence 1 to sequence 48. The primer combination provided by the invention can be used for detecting whether a to-be-detected bacterium is Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus dysgalactiae, Streptococcus uberis, Salmonella typhimurium, Proteus mirabilis or Candida albicans, and can be used for detecting whether a to-be-detected sample contains Escherichia coli and / or Klebsiella pneumonia and / or Pseudomonas aeruginosa and / or Streptococcus dysgalactiae and / or Streptococcus uberis and / or Salmonella typhimurium and / or Proteus mirabilis and / or Candida albicans. The primer combination provided by the invention is used for identifying and detecting the 8 environmental pathogens of dairy cow mastitis, has high specificity and high sensitivity, and can realize simple, rapid and accurate detection, thus having great popularization value.

The invention provides a Chinese herbal medicine complex enzyme detergent as well as a preparation method and application thereof. Raw materials of the Chinese herbal medicine complex enzyme detergent include a Chinese herbal medicine composition and a complex enzyme composition. According to the invention, Chinese herbal medicines specially owned by China are combined with enzyme, the capability of removing bloodstains and spots of enzyme is utilized, the Chinese herbal medicines with the characteristic of inhibiting Salmonella, colibacillus and the like are screened, and then the enzyme and the Chinese herbal medicine are compounded, so that the guarantee period of eco-eggs is prolonged under the synergistic effect of the enzyme and the Chinese herbal medicines; besides, the bloodstains and spots can be resolved completely, so that the soil removal efficiency can be improved.

The invention belongs to the technical field of genetic engineering, and particularly relates to a porcine interferon family gene and application thereof in a blue-ear porcine disease resistant medicament and a vaccine adjuvant. The porcine interferon gene is taken from a home pig genome and has anti-virus effect, and the amino acid sequence of the porcine interferon gene is SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7 or SEQ ID No. 9; and the medicament and the vaccine adjuvant are a recombinant plasmid obtained by cloning the home pig interferon gene to an eukaryotic expression vector through molecular biology technology and a live bacterial vaccine obtained by transferring the plasmid to a low virulent strain of Salmonella choleraesuls. Animal experiments prove that the medicament and the adjuvant have good safety and remarkable effect. After a home pig is directly injected, the toxicity is removed on the same day, and the protective rate can reach 80 percent. When the adjuvant is used with a blue-ear disease inactivated vaccine and after the home pig is subjected one-time immune injection, the toxicity is removed in 28 days, and the protective rate can reach 80 percent.

The invention discloses a multiple rapid detection method of two food-borne pathogenic bacteria and detection primer groups as well as a kit, wherein the sequences of the upstream and downstream primers of an outer primer and the upstream and downstream primers of an inner primer in a quick detection primer group for salmonella are as shown in SEQ No.1, SEQ No.2, SEQ No.3 and SEQ No.4, respectively; and the sequences of the upstream and downstream primers of an outer primer and the upstream and downstream primers of an inner primer in a quick detection primer group for staphylococcus aureus, and the sequence of a loop primer for improving amplification efficiency are as shown in SEQ No.5, SEQ No.6, SEQ No.7, SEQ No.8 and SEQ No.9. The multiple rapid detection method provided by the invention realizes rapid detection of the DNAs of salmonella and staphylococcus aureus in the same system and under the same reaction conditions by using multiple LAMP detection technology in combination with a melting curve analysis method.

The invention discloses a kind of appraisal bacterium, specially appraises the monk fungus (Salmonella), produces the uninuclear cell Liszt fungus (Listeria monocytogenes) the examination method. First (YAU_DSL1) separately uses the monk fungus and the backwoods coli the thermal crack solution to withdraw respective DNA template, produces the uninuclear cell Liszt fungus to use the enzyme solution to withdraw the DNA template, then carries on disposable PCR to expand increases the specific many genes, finally passes through the electricity to swim the appraisal. The invention through sample DNA, disposable at the same time to the monk fungus histidine transportation operon gene which possibly has, produces the uninuclear cell hemolysin gene to expand increases (YZU_DSL1) the material particle carries on with the certain existence backwoods coli expands increases, forms the massive genes duplication fragment, quite is convenient, can appraise whether for the monk fungus, produces the uninuclear cell Liszt fungus, and makes the judgement to the negative result reacting system accuracy. The invention more classical method bacterium separation, the appraisal and the blood serum school grades testing method fast, is accurate.

The invention discloses a multiple PCR (Polymerase Chain Reaction) detection method for four food-borne pathogens, a detection primer set and a kit. The multiple PCR detection method comprises the following steps of: performing multiple PCR reactions on extracted bacterial genome DNA (Deoxyribonucleic Acid) in a sample to be detected in a same reaction system by utilizing rapid detection primer sets of salmonella, shigella, staphylokinase and Listeria monocytogenes, wherein the rapid detection primer sets are obtained through analytic design, and then performing electrophoretic analysis on reaction products to determine whether the sample contains the salmonella, shigella, staphylococcus aureus or Listeria monocytogenes. Each detection primer set has strong specificity and can be used foraccurately detecting the genome DNA of the salmonella, shigella, staphylococcus aureus and Listeria monocytogenes in the same reaction system.

The invention discloses Bacillus pumilus JY2 with the preservation number of CGMCC NO. 9150. The Bacillus pumilus JY2 is sampled from a winter wheat field (N 72 degrees 50 minutes 70.01 seconds E40 degrees 26 minutes 51.73 seconds) in an Osh race course of Kyrgyz Republic and is subjected to separating, screening and physiological and biochemical identification. The Bacillus pumilus JY2 with the preservation number of CGMCC NO. 9150, provided by the invention, has a good application effect on inhibiting pathogenic escherichia coli and salmonella, has a remarkable technical effect on applications in feeding microbioecologics and has broad application prospect in feed additive industries.

The invention relates to bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens in prevention of diarrhea of weaned piglets. The invention provides a bacillus amyloliquefaciens strain BA40, which can be used for inhibiting common porcine intestinal pathogens, namely escherichia coli K88, salmonella typhimurium and staphylococcus aureus, and has the capabilities of resisting acid, bile salt and pancreatin. After the bacillus amyloliquefaciens is activated, the viable count of the bacillus amyloliquefaciens reaches 108-109 CFU / mL, 6-day-old suckling piglets are subjected to intragastric administration through the mouth, and weaning is performed after 1 mL of the suckling piglets are continuously administrated to the stomach for 24 days. The bacillus amyloliquefaciens provided by the invention can improve weaning weight gain of piglets of pigs and diversity of intestinal flora, reduce diarrhea of the weaned piglets, reduce diarrhea rate, reduce inflammatory factors, promote nutrient absorption and improve oxidation resistance and disease resistance of organisms, and provides a new way for nutrient regulation and control under antibiotic-free breeding.

Use of flagellins from the genus Marinobacter as vaccine adjuvants. This invention is based on the use of two recombinant flagellins, F and FR, from the species Marinobacter algicola (DG893T strain), as vaccine adjuvants capable of developing a specific immune response, against peptides or proteins fused to said flagellins, or administered unfused jointly with the flagellins. This invention also describes new combined vaccination strategies, based on the two Marinobacter algicola flagellins and the Salmonella typhimurium flagellin.

The invention discloses a food foodborne pathogenic bacteria rapidly identifying method. The food foodborne pathogenic bacteria rapidly identifying method comprises the steps of 1) DNA (deoxyribonucleic acid) template extraction, 2) primer screening and reaction system optimization including selecting the specific genes of nuc, invA and prfA of staphylococcus aureus, salmonellas and listeria monocytogenes to separately design primer pairs, 3) PCR (polymerase chain reaction) amplification, 4) PCR-DHPLC (polymerase chain reaction-denaturing high performance liquid chromatography) detection of strain DNA, during which only strains coinciding with target amplification can be detected with amplification absorption peaks while other non-targeted amplification bacteria detected to be negative. The food foodborne pathogenic bacteria rapidly identifying method can detect three foodborne pathogenic bacteria in food when applied every single time, thereby saving time and consumable agent, simplifying operation, avoiding excessive artificial errors and achieving high sensitivity.

The invention discloses an inert carrier indirect agglutination test detection system and application thereof. The system comprises inert carrier bacteria S9 and a complex S9-P which is shown and expressed on the surface of the inert carrier bacteria S9 and carries a P-antibody factor. The system only carries the P-antibody factor, is single in component and specific in targeting, generates macroscopic positive particle agglutination reaction with whole blood or serum of chicken infected by pullorum disease and salmonella typhimurium under a certain concentration condition, and does not generate non-specific cross agglutination reaction with chicken-derived serum and whole blood of different backgrounds infected by non-pullorum disease and salmonella typhimurium. The S9-P is based on a glass plate agglutination reaction operation platform. The S9-P is simple and convenient to operate, sensitive and rapid in reaction, macroscopic in agglutination reaction particles and clear and easy inresult judgment, tests and result judgment are completed within two minutes, and the S9-P is suitable for targeted specific detection of pullorum disease and salmonella gallinarum infection in chicken flocks and has a good application prospect in on-site monitoring and diagnosis of the chicken flocks.

The invention provides an attenuated Salmonella vaccine vector comprising one or more heterologous polynucleotides that encode immunogenic Chlamydial peptides. In one embodiment, the attenuated Salmonella vaccine vector comprises aroC and ssaV attenuating mutations. The heterologous polynucleotides encoding the immunogenic Chlamydial peptides can be under the control of an inducible promoter such as a Salmonella ssaG promoter. In one embodiment of the invention, the immunogenic Chlamydial peptide is a PmpG peptide, for instance, a CT110, CT84 or CT40 peptide.