22 results about "Salmonella stanley" patented technology

This invention provides a method for monitoring and evaluating the risk of pathogenic bacteria transmission in poultry farm feces, belonging to the field of livestock and poultry biosecurity control technology. The method includes evaluating the risk of Salmonella transmission during the poultry physiological cycle, assessing the risk of antibiotic resistance associated with drug administration behavior, and monitoring the function during composting based on microbial community structure and metabolic function. This invention can accurately locate the highest-risk nodes of pathogen contamination and antibiotic resistance evolution during the breeding cycle, solving the problem of inaccurate identification of risk sources in existing technologies and avoiding poultry product contamination due to missed risk assessments. It overcomes the single-dimensional limitation of existing technologies that only detect the quantity of pathogens, comprehensively evaluating pathogen risk from phenotypic, molecular, and functional levels. The assessment results are more in line with the actual biosecurity needs of poultry farming, ultimately reducing the safety risks of poultry product contamination and the spread of antibiotic resistance.

A vaccine comprising effective amounts of i) E. coli colonization factor antigens comprising CFA / I, CS3, CS5 and CS6 antigens; ii) a heat labile enterotoxin B subunit antigen being an LCTBA protein; and iii) an adjuvant being double mutant (R192G / L211A) of E. coli heat-labile toxin (dmLT), for use in inducing protective immunity against diarrheal disease caused by Salmonella spp. and / or Shigella spp.

The application discloses a bacteriostatic type of liquor dreg containing Zhangzhou Bacillus GBW-NX and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou Bacillus GBW-NX is CGMCC No. 27402. It is proved through experiments that the Zhangzhou Bacillus GBW-NX has the bacteriostatic effect on inhibiting Escherichia coli, Salmonella and Staphylococcus aureus. The Zhangzhou Bacillus GBW-NX is placed in a solid culture medium containing the liquor dreg to be fermented and enzymolyzed to prepare the bacteriostatic type of liquor dreg. The bacteriostatic substance of the bacteriostatic type of liquor dreg is a bacteriocin. It is also proved that the bacteriostatic type of liquor dreg can improve the intestinal function of ruminants including lactating cows, and can also improve the milk yield of the cows, thereby widening the application range of the Zhangzhou Bacillus GBW-NX, providing a new direction for the feeding of ruminants, and having important significance for the breeding of ruminants.

The invention discloses a rabbit source fermentation lactobacillus mucus RLF77 and an application of the rabbit source fermentation lactobacillus mucus RLF77. The strain is separated from intestinal tracts of weaned young rabbits and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252224. The bacterial strain has the excellent characteristics of being used as a probiotic, being resistant to gastrointestinal tract environment and high in intestinal epithelial cell adhesion rate, and being capable of effectively inhibiting various pathogenic bacteria such as escherichia coli, salmonella and the like. Animal experiments show that the production performance of weaned young rabbits can be remarkably improved, the diarrhea rate can be reduced, the survival rate and the oxidation resistance can be improved by feeding the rabbit-derived RLF77 strain, and the effect is superior to that of non-rabbit-derived probiotics. The invention provides a new strain resource for developing green breeding products for replacing antibiotics.

The invention provides an antibacterial peptide ATMP23 mutant with improved antibacterial activity and application of the antibacterial peptide ATMP23 mutant in preparation of an antibacterial agent. The amino acid sequence of the antibacterial peptide ATMP23 provided by the invention is shown as SEQ ID NO: 1, amino acid mutation and screening are carried out on the basis of the amino acid sequence, and antibacterial peptide mutants A23-M4, A23-M5 and A23-M6 with significantly improved antibacterial activity are obtained. The antibacterial peptide ATMP23 mutant provided by the invention is small in molecular weight, convenient to artificially synthesize and low in cost, and has a good bactericidal effect on clostridium welchii, staphylococcus aureus, streptococcus, vibrio parahaemolyticus, vibrio alginolyticus, vibrio splendidus, salmonella, escherichia coli and the like; when being applied to livestock and aquatic feed, the feed conversion ratio of livestock and aquatic products can be remarkably reduced, the growth performance and immune function indexes of bred animals can be improved, and the feed additive can be widely applied to the industries of feed, food, medicine and the like.

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

The invention establishes a method for simultaneously detecting vibrio parahaemolyticus, staphylococcus aureus, listeria monocytogenes, salmonella, shigella, bacillus cereus and vibrio alginolyticus on the basis of multiple PCR (Polymerase Chain Reaction) combined with a gene chip technology. The method comprises the following steps: selecting a heat-labile hemolysin gene of vibrio parahaemolyticus, a heat-resistant nuclease gene of staphylococcus aureus, a coded hemolysin O gene hlyA of listeria monocytogenes, an invasive protein A gene of salmonella enteritidis and an invasive plasmid antigen H gene of Shigella to carry out quintuple PCR (Polymerase Chain Reaction) amplification; bacillus cereus non-hemolytic toxin genes and vibrio alginolyticus similar Rpos sigma factor genes are selected for double PCR, two groups of multiplex PCR amplification products are hybridized with specific probes on the chip at the same time, and seven food-borne pathogenic bacteria can be detected at the same time. The method for detecting pathogenic bacteria by using the gene chip is high in specificity, high in sensitivity and strong in reliability.

This invention discloses a multilayer microsphere for the prevention and / or treatment of bacterial diarrhea, its preparation method, and its applications, relating to the field of biomedical technology. The multilayer microsphere has a three-layer core-shell structure: the core layer is loaded with *Lactobacillus plantarum* and sodium butyrate, the middle layer is a chitosan coating layer, and the outer layer is a sodium alginate coating layer. This invention has found that *Lactobacillus plantarum* and sodium butyrate have a significant synergistic antibacterial effect, effectively inhibiting the proliferation of pathogenic *Escherichia coli* and *Salmonella*. In vivo experiments have confirmed that the multilayer microsphere can effectively restore the body weight of diarrhea-prone animals, repair pathological damage to the jejunum and colon, and alleviate intestinal inflammation at the molecular level by inhibiting the activation of NF-κB and MAPK inflammatory pathways, downregulating the expression of pro-inflammatory factors IL-1β, IL-6, and TNF-α, and upregulating the level of the anti-inflammatory factor IL-10. The multilayer microsphere provided by this invention can serve as a novel, green, and safe formulation suitable for the prevention and control of bacterial diarrhea in livestock and poultry farming.

The invention discloses an immortalized goat bone marrow-derived macrophage for expressing an SV40LT gene and application of the immortalized goat bone marrow-derived macrophage, and belongs to the technical field of bioengineering. The immortalized goat bone marrow-derived macrophage provided by the invention is obtained by knocking an immortalized gene SV40LT into a primary goat bone marrow-derived macrophage ROSA26 site through a CRISPR-Cas 9 system, and the immortalized goat macrophage provided by the invention is an immortalized goat macrophage cell strain which is suitable for being infected with salmonella and brucella and can be continuously and stably passaged; the method can be effectively applied to immunological research on goat brucella infection resistance and production of brucella vaccines.

A genetically modified Salmonella cell (GMSC) engineered to display pattern-associated and danger-associated molecular patterns to recruit and enhance innate immunity and exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and / or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

The invention belongs to the technical field of microorganisms, and particularly relates to lactic acid bacteria and application thereof. The bacterial strain is Ligilobacillus sativus SunLab-GHL01 (L.sativus GHL01 for short), is separated from lungs of high-production-performance Ailijiai broiler chickens and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the bacterial strain is CCTCC NO: M 20251725. The invention further discloses a preparation method of the high-production-performance Ailijiai broiler chicken with high-production-performance Ailijiai broiler chicken with high-production-performance Ailijiai broiler chicken with high-production-performance Ailijiai broiler chicken. Through morphology, physiology and biochemistry, 16S rRNA sequence identification and whole genome sequencing analysis, the taxonomic status and functional characteristics of the 16S rRNA are defined. The strain has good acid resistance, cholate resistance and acid production capacity, has broad-spectrum antibacterial activity, and can effectively inhibit pathogenic bacteria such as escherichia coli, pathogenic escherichia coli, salmonella, staphylococcus aureus and pseudomonas aeruginosa. Animal experiments show that the poultry feed conversion rate and the anti-stress capability can be remarkably improved by atomizing the strain. The strain and the application thereof can be used for improving the health condition and the production performance of poultry under the intensive breeding condition, and have application prospects in the fields of feed additives, probiotic preparations, animal health products and the like.

The utility model discloses a method for using nano-enhanced loop-mediated isothermal amplification-recombinase polymerase amplification (LAMP-RPA) biosensor kit for rapid cage-side detection of Salmonella species in poultry farms. The method involves processing fecal sample collected from chickens, rehydrating the assay components with Silver nano particles (AgNPs) solution, activating the assay with Magnesium acetate (Mg(OAc)2). Interpreting results is achieved by a naked eye observation of change in color of the mixture. A positive result is indicated by the retention of the original dark gray to dark violet color of the mixture, otherwise, it is signaled by the appearance of a colorless to yellow mixture. This was done in a single-tube reaction, enabling a rapid, equipment-free, and userfriendly nucleic acid-based diagnostic platform ideal for on-site deployment. Diagnostic validation of specificity of the biosensor kit exhibited good results. Moreover, it can detect down to 64 picogram of DNA per reaction or a 10?³ dilution.

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp. / Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1 / Shiga toxin A, or Escherichia coli stx2 / Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

This invention discloses a sodium caseinate-based Pickering emulsion, its preparation method, and its application, belonging to the field of vaccine adjuvant technology. This invention is the first to propose using Salmonella Typhimurium flagellin and sodium caseinate as raw materials to formulate a Pickering emulsion. The prepared Pickering emulsion has the characteristics of small particle size, good biocompatibility, and high stability, and can induce a high immune response in the body. This invention is also the first to propose the application of the Pickering emulsion formulated using Salmonella Typhimurium flagellin and sodium caseinate as raw materials as a vaccine adjuvant. The Pickering emulsion prepared by this invention has a simple preparation process, requiring only mixing and homogenization, and can effectively load the model antigen OVA. This emulsion has good cellular safety and can promote the endocytosis of exogenous antigens by macrophages. The emulsion can induce the body to produce high levels of antibody titers, with antibody titers reaching more than 120,000 times.

The invention provides a primer group and a kit for detecting four bacterial abortion diseases of sheep, belongs to the technical field of biological detection, establishes a multiplex PCR (polymerase chain reaction) detection method for sheep brucellosis, sheep abortion salmonellosis, listeriosis and chlamydia disease and a kit thereof, and has the characteristics of high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like. The rapid differential diagnosis of four sheep bacterial abortion diseases can be realized through one-time PCR amplification, and the method has important advantages in the field of sheep disease diagnosis and identification.

The invention discloses a pediococcus pentosaceus D17-sourced cytoplasmic membrane vesicle and application thereof, and belongs to the technical field of microorganisms. The pediococcus pentosaceus D17 with excellent probiotic characteristics is separated from fresh excrement of Lichuan horses for the first time, the preservation number of the pediococcus pentosaceus D17 is CCTCC (China Center For Type Culture Collection) NO: M20252051, and meanwhile, the application of the pediococcus pentosaceus D17 and the cytoplasmic membrane vesicles of the pediococcus pentosaceus D17 in resisting salmonella infection is researched. Experimental verification shows that the separated and screened pediococcus pentosaceus D17 and the cytoplasmic membrane vesicles thereof have the effect of resisting salmonella infection and can promote expression of tight junction protein, and the treatment effect of the cytoplasmic membrane vesicles derived from the pediococcus pentosaceus D17 is superior to that of the pediococcus pentosaceus D17. Due to the fact that the cytoplasmic membrane vesicles are prepared through centrifugation of bacterial liquid supernatant, the cytoplasmic membrane vesicles are safer and free of drug resistance risks, and a new thought and a new approach are provided for application of the cytoplasmic membrane vesicles sourced from pediococcus pentosaceus to preparation of salmonella infection resisting products.

The invention discloses a lactobacillus amyloliquefaciens and probiotic microbial inoculum and application of the lactobacillus amyloliquefaciens and probiotic microbial inoculum. The lactobacillus amyloporus is named as lactobacillus amyloporus YI39, and the preservation number of the lactobacillus amyloporus YI39 in the China Center for Type Culture Collection is CCTCC (China Center for Type Culture Collection) NO: M2026104; the nucleotide sequence of 16SrDNA (deoxyribonucleic acid) of the gene is SEQ ID NO. 1. The lactobacillus amyloliquefaciens YI39 provided by the invention is separated from ileum contents of 28-day-old healthy and rapid yellow-feathered broilers, has strong probiotic characteristics, and has relatively good capabilities of inhibiting growth of salmonella pullorum, avian pathogenic escherichia coli, A-type clostridium perfringens and staphylococcus aureus; the body of the broiler chicken can be effectively regulated and controlled, so that the chicken necrotic enteritis caused by clostridium perfringens infection is prevented.

The application relates to the technical field of food safety, and discloses a food microbial contamination rapid detection method fusing a knowledge graph and deep learning, which comprises a data acquisition module, a knowledge graph engine, a deep learning calculation unit, a dynamic optimization module, a visual interactive terminal and a traceability management platform. By adopting microfluidic chip and impedance sensing combined technology, the traditional long-time detection cycle is shortened, the real-time monitoring demand of cold chain logistics is met, a spectrum-bioelectric-microscopic image three-mode space-time alignment model is created, a dimensional gap is eliminated through feature space mapping, the recognition accuracy of complex matrix pollution is improved, a rule engine driven by an industry knowledge graph is constructed, model dynamic updating is realized through an incremental learning module, the detection rate of new Salmonella varieties is improved, and the problem of new pathogen missed detection rate is solved.

This invention proposes a droplet digital PCR method for simultaneously detecting the quantity of four foodborne pathogens. The steps include: 1. Preparing the DNA sample to be tested; 2. Preparing primers and probes for the four bacteria to be tested; the four bacteria to be tested are Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus; 3. Using a ddPCR amplification reaction system, which includes the primers and probes for the four bacteria to be tested in step 2, amplifying the DNA sample to be tested in step 1, detecting the amplification products, and obtaining the quantitative results of the four bacteria. This invention can simultaneously detect four pathogenic microorganisms: Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus. It establishes a precise, rapid, and absolute quantitative detection system for the simultaneous detection of these four pathogens using multiplex ddPCR technology, exhibiting high specificity, high precision, and strong repeatability.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LT304 and application thereof.The lactobacillus plantarum strain LT304 is separated from an acid material, and a fermentation product of the strain has an antibacterial effect on one or more of salmonella enteritidis, escherichia coli and listeria monocytogenes; after fermentation of the strain LT304, compared with fermentation of other same strains, enzyme activities of PAL, POD, PPO, APX and CAT in pickled Chinese cabbages are reduced, enzyme activity of LOX is increased, and the pickled Chinese cabbages are brightest; the lactobacillus plantarum LT304 strain has an obvious anti-browning effect on the leaf mustard pickled Chinese cabbages, and after histidine and / or proline are / is added for fermentation, the brightness can be further improved, and the color of the pickled Chinese cabbages is better, so that the LT304 strain can be used for preparing the pickled Chinese cabbages with better color, can effectively prevent browning, provides a new thought for follow-up development of anti-browning agents, and has wide application prospects. The fermentation liquor has the value of research and development of antibacterial peptides.

The invention discloses a Rongchang pig source ZH30 strain and application thereof, and belongs to the technical field of agricultural livestock feed additives. The ZH30 strain provided by the invention has bacteriostatic activity on a plurality of common pig pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella typhimurium, salmonella pullorum and enterococcus faecalis, is wide in antibacterial spectrum, and is beneficial to reducing the load of pathogenic bacteria in intestinal tracts of pigs. Meanwhile, the ZH30 strain is sensitive to streptomycin, gentamicin and ampicillin, infectious microbe pollution can be conveniently controlled through a conventional antibiotic means in the fermentation and preparation production process, and meanwhile the drug resistance spreading risk is reduced. As a functional strain of a probiotic preparation for pigs, the ZH30 strain disclosed by the invention can improve the intestinal health of pigs and improve the mechanical barrier integrity of the intestinal tracts, has potential application values of reducing the diarrhea risk of piglets and improving the feed utilization rate and the production performance, and is of great significance to the development of animal husbandry and feed industry.

The invention discloses an electrochemical sensor based on aptamer and bacteriophage dual recognition and application of the electrochemical sensor, and belongs to the technical field of food safety detection. The electrochemical sensor provided by the invention comprises a gold electrode modified by a DNA regular tetrahedron nanostructure and a calcined zeolite imidazate framework material anchored with phage and loaded with methylene blue, the DNA regular tetrahedron nanostructure comprises four DNA probes, and the four DNA probes are a chain A, a chain B, a chain C and a chain D; the bacteriophage is a salmonella typhimurium bacteriophage. The electrochemical sensor can perform dual recognition based on the aptamer and the bacteriophage, realizes high sensitivity, high specificity and rapid detection of salmonella typhimurium through the synergistic effect of all functional components, has biosafety prevention and control capability, and can meet the detection requirements of trace pathogenic bacteria in food.