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A method for establishing a mouse model of mastitis inhibited by Klebsiella pneumoniae phage

A Klebsiella, model-building technology, applied in the field of biomedicine, to achieve the effect of inhibiting tissue damage, strong ability to split bacteria, and strong ability to split bacteria

Active Publication Date: 2021-04-16
河北中贝佳美生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problem of shortage of existing technologies, the present invention provides a method for Klebsiella pneumoniae phage to inhibit the establishment of mouse mastitis model, aiming to find a wide lysis spectrum and strong bacteriostasis ability for mouse mastitis , non-toxic Klebsiella pneumoniae phage, providing experimental and theoretical basis for the treatment of bovine mastitis

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  • A method for establishing a mouse model of mastitis inhibited by Klebsiella pneumoniae phage
  • A method for establishing a mouse model of mastitis inhibited by Klebsiella pneumoniae phage
  • A method for establishing a mouse model of mastitis inhibited by Klebsiella pneumoniae phage

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Embodiment Construction

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described examples are only part of the embodiments of the present invention, not all examples.

[0043] When implementing it:

[0044] 1. Experimental materials

[0045] 1.1 Preparation of Klebsiella pneumoniae bacteria liquid

[0046] Klebsiella pneumoniae strains were isolated and preserved in our laboratory. Streak inoculation of Klebsiella pneumoniae into LB solid medium, placed in a constant temperature incubator at 37°C, and cultured overnight. Pick a single colony, inoculate it in BHI liquid medium, place it on a 220r / min shaker at 37°C, and culture it overnight. Adjust the concentration of the bacterial solution to 1×10 3 CFU / mL, 1×10 5 CFU / mL, 1×10 7 CFU / mL three concentrations.

[0047] 1.2 Preparation of Klebsiella pneumoniae phag...

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Abstract

The invention provides a method for establishing a Klebsiella pneumoniae phage inhibiting mouse mastitis model, comprising the following steps: A, preparation of Klebsiella pneumoniae bacterial liquid, B, preparation of Klebsiella pneumoniae phage culture liquid, C, Experimental animal treatment, D, collection and testing of experimental samples. Through this method, the present invention can more accurately find Klebsiella pneumoniae phages with wide lysis spectrum for mouse mastitis, strong bacteriostasis ability and no toxic or side effects, so as to provide experimental and theoretical basis for treating bovine mastitis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for establishing a Klebsiella pneumoniae phage inhibiting mouse mastitis model. Background technique [0002] As an environmental pathogen, Klebsiella pneumoniae is usually isolated from litter, sewage, animal in vitro and feces in farms. Cow mastitis caused by Klebsiella pneumoniae usually occurs during the lactation period of dairy cows. During this period, infection with Klebsiella pneumoniae not only leads to reduced milk production and milk quality, but also causes acute death of dairy cows. At present, cow mastitis caused by Klebsiella pneumoniae is mainly treated with antibiotics, but this kind of treatment often has poor prognosis, repeated attacks, and is likely to cause serious drug resistance. [0003] The economic loss caused by this bacteria on dairy farms is comparable to that caused by Gram-positive bacteria causing mastitis. In large dairy farms i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6851
CPCA01K67/02C12Q1/6851G01N33/6863G01N33/6869C12Q2531/113C12Q2521/107C12Q2563/107
Inventor 韩博石玉祥高健赵文鹏刘钢刘洋张永英朱阵
Owner 河北中贝佳美生物科技有限公司
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