Pyrolysis phycophage MACPNOA1 and application thereof

A technology of cyanophages and algal plaques, which is applied to the lytic cyanophage MACPNOA1 and its application in the control of cyanobacterial blooms, can solve the problems of reducing algae density, affecting primary productivity, and reducing the incidence of photosynthesis, etc. Achieve the effects of wide cleavage spectrum, rapid lysis, and simple culture conditions

Active Publication Date: 2018-12-28
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invasion of cyanophages can affect the changes in the photosynthesis center of their hosts, resulting in a decrease in the incidence of photosynthesis, affecting the primary productivity in water bodies, and significantly reducing the density of algae

Method used

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  • Pyrolysis phycophage MACPNOA1 and application thereof
  • Pyrolysis phycophage MACPNOA1 and application thereof
  • Pyrolysis phycophage MACPNOA1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of cyanophage MACPNOA1 is as follows:

[0032] (1) Preliminary isolation of cyanophages

[0033] The surface water sample was collected from the South Gate Ditch of Ningbo University, Ningbo City, Zhejiang Province, centrifuged at 10,000×g for 30 minutes, the supernatant was filtered through 0.8 μm and 0.45 μm filter membranes, and the filtrate was mixed with the aeruginosa microcapsules in the logarithmic growth phase Algae (M.aeruginosaFACHB-469) were mixed in a volume ratio of 1:2; at the same time, the BG11 algal culture medium was added to Microcystis aeruginosa in the logarithmic growth phase in the same proportion as a control; , the temperature is 25°C, and the light-dark cycle is 12h: 12h, cultivate under the condition of 12h:12h, obtain the yellowed culture fluid, and observe the algae fluid with a microscope to confirm the lysis of the algae ( figure 1 ). The yellowed culture solution was centrifuged at 10000×g for 30 minutes, the sup...

Embodiment 2

[0045] Morphological observation of lytic cyanophages

[0046] The cyanophage suspension obtained in Example 1 was dropped on a copper net, negatively stained with 2% uranyl acetate solution (w / v), and placed under an electron microscope (Hitachi H-7650) to observe the cyanophage. form.

[0047] The cyanophage has a round head structure and a slender non-retractable tail. The capsid diameter of the head is about 40nm, and the length of the tail is about 380nm.

Embodiment 3

[0049] Analysis of cyanophage genomes

[0050] The cyanophage isolated and purified in Example 1 was taken, and after the nucleic acid was extracted, they were treated with DNase I, Rnase A and S1 single-stranded nuclease (S1 Nuclease) respectively, and the treatment method was carried out according to the instructions of Takara. The processed sample was subjected to agarose nucleic acid electrophoresis, and the obtained spectra were as follows: image 3 As shown, where Lane 1: DNase I enzyme treatment, Lane 2: Rnase A enzyme treatment, Lane 3: S1 Nuclease enzyme treatment, Lane 4: Untreated nucleic acid. The result shows that this cyanophage nucleic acid can be digested by DNase I, but can not be digested by RNase A enzyme and S1 Nuclease enzyme. The results showed that the genome of cyanophage MACPNOA1 was double-stranded DNA (dsDNA); the genome DNA of MACPNOA1 was sequenced by next-generation sequencing. According to the "Classification of Viruses-The Eighth Report of the...

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Abstract

The invention discloses pyrolysis phycophage MACPNOA1 and a separation method and application thereof, and relates to biological treatment of eutrophic water. MACPNOA1 is preserved in China General Microbiological Culture Collection Center in October 24th, 2016, and the preservation number is CGMCC No.12996. MACPNOA1 has a wide alge-lysing spectrum, and widely lyses common water-blooming cyanobacteria; when MACPNOA1 is added into cyanobacterial water-blooming liquid belonging to seven categories of Microcystis, Aphanizomenon, Chroococcus, Planktothrix, Nostoc, Oscillatoria and Anabaena, MACPNOA1 has strong pyrolysis effects on all the measured seven categories. When MACPNOA1 is applied to algal bloom treatment, the obvious effect of protecting animals in water is achieved. The pyrolysis phycophage MACPNOA1 has the advantages of being safe, efficient and quick.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a lytic cyanophage MACPNOA1 and its application in the control of cyanobacteria blooms. Background technique [0002] With the intensification of human activities in the lake basin, a large amount of nutrients enter the water body through various channels. The Organization for International Economic Development Cooperation (OECD) defines this phenomenon as "a series of changes such as the increase in the productivity of algae and aquatic plants and the decline in water quality due to the increase in nutrients in the water body, thereby affecting the use of water" as the phenomenon of nutrient enrichment. Nutritization. [0003] A common phenomenon associated with eutrophication is that many phytoplankton, especially those algae with buoyancy or motility, usually grow excessively, form algal blooms, and release toxins, resulting in the decline of water quality and a se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02C02F3/34C12R1/92
CPCC12N7/00C02F3/34C02F2103/007C12N2795/10351C12N2795/10321
Inventor 顾叶华李登峰严小军
Owner NINGBO UNIV
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