228 results about "Cyanophage" patented technology

The invention provides a genetically modified Cyanobacteria having a construct comprising DNA fragments encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) enzymes obtained from the Zymomonas mobilis plasmid pLOI295. The Cyanobacteria are capable of producing ethanol in recoverable quantities of at least 1.7 mumol ethanol per mg of chlorophyll per hour.

The invention provides a genetically modified Cyanobacteria having a construct comprising DNA fragments encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) enzymes obtained from the Zymomonas mobilis plasmid pLOI295. The Cyanobacteria are capable of producing ethanol in recoverable quantities of at least 1.7 mumol ethanol per mg of chlorophyll per hour.

The invention relates to a microbe germ composition, a cyanophyte biological germ leaven which contains the microbe germ, a cyanophyte fermentation nutrient, a cyanophyte biological organic fertilizer and a method for preparation, wherein the microbe germ composition contains plant lactobacillus, bacillus subtilis and swamp Rhodopseudomonas, the cyanophyte biological germ leaven is prepared by microbe germ composition and base, the cyanophyte fermentation nutrient is prepared by cyanophyte biological germ leaven and cyanophyte, the cyanophyte biological organic fertilizer is prepared by cyanophyte fermentation nutrient, organics base and water-loss reducer. The microbe germ composition is provided with the ability for fermenting solid cyanophyte, which can transform nutriment in the cyanophyte into the material for crops. The cyanophyte fermentation nutrient can be superior animal feed. The cyanophyte biological organic fertilizer is provided with crop pest control effect, which adds the soil organics content and improves nutrient utility to accelerate the plant growth belonging to natural superior organics fertilizer.

Systems and methods for maintaining the dominance of Nannochloropsis in an algae cultivation system are provided. Exemplary methods include applying an effective amount of a disinfectant to Nannochloropsis growing in an algae cultivation system. Another method for maintaining the dominance of Nannochloropsis in an algae cultivation system includes adjusting a salinity in the algae cultivation system to between approximately 0.5 PPT and 28 PPT. In a further method, temperature of the algae cultivation system may be adjusted to between approximately 21 and 32 degrees Celsius (“° C.”). According to yet another method for maintaining the dominance of Nannochloropsis in an algae cultivation system, a salinity in the algae cultivation system may be adjusted to below that of seawater for a first predetermined period of time, and then the salinity in the algae cultivation system may be adjusted to a higher salinity for a second predetermined period of time.

The invention relates to a blue algae water bloom active carbon and a preparation method thereof. The active carbon is produced by drying the blue algae water bloom alga mud at the hign temperature of 250 to 600 DEG C for 0.5 to 2 hours. The invention starts a new trail for utilizing the blue algae water bloom as a resource. The method can effectively produce active carbon with blue algae water bloom, which can not only save resources but also is favorable to protect the ecological environment and finally the purpose of treating pollution and reducing the pollution treatment cost can be realized.

The present invention includes compositions and methods for making and using cyanobacteria that include a portion of an exogenous cellulose operon sufficient to express cellulose. The compositions and methods of the present invention may be used as a new global crop for the manufacture of cellulose, CO2 fixation, for the production of alternative sources of conventional cellulose as well as a biofuel and precursors thereof.

The invention discloses a method for preparing phycocyanin by using bloom cyanophyte. Said invention uses the fresh bloom cyanophyte as raw material, and adopts the following steps: first step, extracting crude filtrate; dehydrating fresh bloom cyanophyte, adding buffer solutio, mixing them uniformly, frezing, takingout and thawing at room temperature, freezing and thawing for 3-5 times and filtering by using plate and frame; second step, ultrafiltering and purification; using hollow fibre ultrafiltration device whose trapping molecular weight is 1000-200000 daltons to remove various substances with smaller molecular weight from crude extract passed through microfiltration by using ultrafiltering membrane; third step, settling-clearing purification; removing most of small molecules from phycocyanin portion purified by means of ultrafiltration, at the same time making concentration and storing the concentrated phycocyanin portion at a certain temperature; and fourth step; hydroxyapatite column chromatography to make purification.

The invention discloses a method for extracting algae glycoproteins. The method for extracting the algae glycoproteins comprises the following step: mixing a certain amount of the mixture of algae, blue-green algae or the mixture of alga powder and water with the water solution with specific fungi. The algae glycoproteins extracted by the method have the functions of preventing and treating the respiratory diseases, and also have functions on broad-spectrum antiviral, anti-inflammatory, anticoagulin, bacteriostasis, refrigeration and immune adjustment.

A removing method of overwintering water-blooming cyanobacteria provenances in large-scale shallow lakes is disclosed. In the overwintering period and the recovery period, algae-controlling agents are respectively used in distribution areas of overwintering water-blooming cyanobacteria provenances twice to perform background removal of overwintering cyanobacteria provenances in water and sediment, wherein the first background removal is performed in the overwintering period, and the second background removal is performed in the recovery period before cyanobacteria recover; and the algae-controlling agent comprises rice straw and H2O2 with effective concentrations. The invention is based on the self-growth and recovery rule of algae; environment-friendly algae-controlling agents are used for prevention and control treatment at provenance regions for cyanobacteria growth and in bottleneck periods - the overwintering and the recovery periods; overwintering water-blooming cyanobacteria provenances in large-scale shallow lakes can be effectively removed; cyanobacteria water bloom can be treated from the source; and the method is a new approach for the prevention and control of the generation of harmful algae water bloom.

The invented prodn. process contains steps of: (1) raw material algal bloom blue green algare is concentrated, dewatered, dried and crushed to obtain algare powder; (2). said powder is soaked in mineral ether at room temp. to break the cells, and continuously soaked after a night, refluxing in water bath, then removing leavings of the cell to obtain gamma-linoleates mixed ester; (3). said ester is saponified after adding saponifier in water bath, then cooled to room temp., (4). adjusting said solution pH value to 3-4, then methoxycarbonylation and urea packing reaction to abtain gamma-linoleic acid. Advantages are: simple process, high obtg. rate, easy to obtain raw material, with social, environment and economic benefits.

The invention provides a method for simultaneously separating and purifying reagent-grade phycocyanin and allophycocyanin. The method comprises the following steps: breaking walls of blue-green algae and carrying out solid-liquid separation to obtain a phycobiliprotein crude extraction solution; carrying out two-step salting-out to obtain sediment; dissolving the sediment by utilizing a phosphate buffering solution; by taking the phosphate buffering solution as a dialysis solution, dialyzing a dissolved solution so as to obtain a dialyzed phycobiliprotein solution; finally, carrying out cellulose column chromatography and hydroxylapatite column chromatography to obtain the reagent-grade phycocyanin and the reagent-grade allophycocyanin. According to the method provided by the invention, water-bloom fresh blue-green algae is used as a raw material and the reagent-grade phycocyanin and the reagent-grade allophycocyanin are separated and purified by adopting salting-out combined with column chromatography; compared with a traditional chromatography process, high-purity phycocyanin and allophycocyanin can be simultaneously extracted and purified under the premise of considering the recovery rate of protein, and the efficiency of comprehensively extracting and purifying high-value protein in blue-green alga cells is improved and can be amplified.

The invention relates to a method and treatment agent for preventing and controlling blue-green algae. A cyanophage bacterium S agent is a culture solution with the solid content of 0.5-2 percent, which is prepared from spirulina powder, NaCl and purified water; a biological substrate modified agent of K agent is prepared by compounding saccharomycetes, bacillus subtilis and cyanophage bdellovibro; and an algal removing water purifying agent of L agent is prepared from 40wt% of sodium carbonate, 50wt% of fourth generation of polyamidoamine and 10 wt% of sodium bentonite. The invention overcomes the defects of two methods by using beneficial bacterium which scrambles nutrition with the blue-green algae and cyanophage capable of phagocytosing the blue-green algae. The invention is a system engineering for preventing and controlling blue-green algae, which organically combines a biological method and a chemical method and can prevent and control blue-green algae comprehensively with proper cost and favorable practicability and stability; and the blue-green algae is controlled by using a microbial method at early stage with obvious implementation effect , since a cell wall of the blue-green algae is thinner and cyanophage can phagocytose algae quickly; in addition, since a water body has high eutrophication degree in summer, comprehensive treatment can achieve good effect.

The invention discloses a recombinant blue-green algae, which is prepared by a method comprising the step of introducing genes related to butanol synthesis shown in (1) and (2) into yhe chroococcaceae blue-green algae, wherein (1) the genes related to butanol synthesis comprise encoding genes of the following enzymes: electron transfer flavoprotein A subunit, enoyl hydrase, butyryl-COA dehydrogenase and alcohol dehydrogenase; and (2) the genes related to butanol synthesis comprise encoding genes of the following enzymes: electron transfer flavoprotein B subunit, the enoyl hydrase, the butyryl-COA dehydrogenase and the alcohol dehydrogenase. The invention realizes the blue-green algae to transform inexhaustible solar energy and greenhouse gas carbon dioxide on the earth into the energy product butanol, avoids wasting grains and other organic matters in the energy regeneration process, and at the same time is beneficial for environment protection. Therefore, one of ideal methods for realizing the sustained and healthy development of clean and renewable energy sources is to utilize the recombinant blue-green algae of the invention to produce the butanol.

The invention provides an expression vector containing a recombined blue algae antiviral protein N nucleotide sequence, a host cell containing the expression vector and a method for preparing recombined blue algae antiviral protein on the basis to obtain the recombined blue algae antiviral protein and further proves that the expression vector and / or the host cell and / or the recombined blue algae antiviral protein can be applied to prepare an antiviral medicament. The method of the invention overcomes the disadvantages of low yield, an easily formed inclusion body and difficult purification and the like in the prior art. Peptide mass fingerprinting of the recombined CVN protein prepared by the method is totally consistent with theoretical peptide mass fingerprinting; and an antiviral experiment proves that the recombined protein has good antiviral activity.

The invention discloses a preparation method for algae endophytic fungi exocellular polysaccharide, and relates to a biological method for synthesizing polysaccharide. According to the preparation method, purified polysaccharide is separated from blue algae endophytic fungi DT06 serving as production strains through shaking table or liquid-state submerged cultivation fermentation, wherein the blue algae endophytic fungi DT06 are used as the production strains; the strains are collected at December 20, 2010 with a collection number of CGMCC 4460; and the preparation method specifically comprises the following steps of: 1, performing liquid fermentation by using the blue algae endophytic fungi DT06; 2, performing exocellular polysaccharide separation; and 3, purifying the polysaccharide to finally prepare the pure fungi exocellular polysaccharide product which is shown as the following structural formula. The endophytic fungi DT06 used in the method are separated from the blue algae; and the defects of narrow raw material source, low probability of separation and high production cost existing in the prior art are overcome.

The invention relates to a complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture as well as a preparation method and an application of the complex microbial inoculant. The complex microbial inoculant comprises fermented Bacillus subtilis MES 810 powder and fermented Bacillus amyloliquefaciens MES812 powder in a mass ratio being 1:1. The complex microbial inoculant is prepared from the fermented Bacillus subtilis powder and the fermented Bacillus amyloliquefaciens powder by sufficient mixing in a mass ratio being 1:1, the viable count of the fermented Bacillus subtilis powder is not lower than 2.0*10<10>cfu / g, and the viable count of the fermented Bacillus amyloliquefaciens powder is not lower than 4.0*10<9>cfu / g. The prepared complex microbial inoculant for preventing and treating cyanobacterial bloom in aquaculture not only has an inhibition effect on cyanophyta algae, but also has no influence on other beneficial algae, thereby being an environment-friendly product.

The invention discloses a preparation method and an application of immobilized algal toxin degrading bacteria. The preparation method of the immobilized algal toxin degrading bacteria comprises the steps: selecting activated carbon fibers as an immobilization material, after carrying out washing, boiling, hydrochloric acid soaking, drying and other pretreatment steps of the immobilization material, adding into an LB culture medium according to a certain specific value, then inoculating a bacterial strain for degrading microcystins (MCs), and carrying out immobilized culture to obtain the immobilized algal toxin degrading bacteria. In the application of the immobilized algal toxin degrading bacteria, while the activated carbon fibers adsorb algal toxins, the algal toxin degrading bacteria immobilized on the activated carbon fibers can degrade the algal toxins, can greatly shorten the time of degradation of the MCs, and simultaneously can biologically regenerate the activated carbon fibers. The bacterial strain for degrading the MCs has the MC-LR degradation rate of up to 91% or more, and is especially suitable for degradation of the MCs generated in a process of extracting phycobiliproteins from water-blooming cyanobacteria.

The invention discloses application of ginkgolic acid in killing of a blue-green algae, application of an alcohol extract of ginkgo biloba sarcotesta in killing of the blue-green algae, a composition and application of the composition in killing of the blue-green algae. It is observed that the ginkgolic acid and a preparation containing the ginkgolic acid have strong activity of killing the blue-green algae from the embodiment of the invention. In addition, the ginkgolic acid is plant source algicide, is taken from the natural world and applied to the natural world, is low in toxicity and residue, and is an algicide which is friendly to the environment, and simple in using method; and the drug resistance is not easily generated.

The invention relates to a method for inhibiting cyanobacterial bloom, belongs to the fields of aquaculture industry and environmental science, and particularly provides a method for inhibiting cyanophyta microcystis bloom, which is characterized by comprising the following steps: (1) monitoring the nitrogen element concentration of water in which cyanophyta microcystis bloom occurs; (2) aerating the water continuously to maintain the oxyty of the water; (3) adding carbonaceous saccharides during aeration, wherein the addition concentration depends on the total nitrogen concentration of the water; (4) adding silicon compounds into the water during addition of the saccharides; (5) ensuring that cyanobacterial bloom particles become smaller and smaller, and the water becomes more transparent after 2 to 3 days; (6) adding saccharides again in the 7th to 10th days to ensure that the cyanobacterial bloom particles become smaller and less. Through the adoption of the method, cyanobacterial bloom in the water is effectively inhibited; the transparency of the water is improved; the significances in environment protection and ecologic protection are both high.

The invention provides a method for biologically treating and transforming blue algae by using insect larvae, and belongs to the technical field of resource environments. The method comprises the following steps of: after the fished blue algae serous fluid or the dehydrated blue algae mud is hydro-thermally treated, adding a little amount of bran into the blue algae serous fluid or the blue algae mud, and pre-fermenting the mixture for 2 days to prepare a culture material for fly larvae, wherein the thickness of the culture material in a culture tray is 5 to 10 centimeters; inoculating 0.3 gram of fly eggs to the surface of each kilogram of culture material, wherein the point-sowing density is 3 to 5 places per square meter; and placing the culture tray in a room with good ventilation and weak rays, culturing the fly eggs for 3 to 5 days, and harvesting the fly larvae serving as feed protein after the fly larvae is mature. The residue is smell-less and loose, greatly reduces the moisture, has rich nutrient, and can be directly used as a biological organic fertilizer. The method for treating the blue algae is efficient and quick and has high economic benefit.

The invention discloses a method for producing isoprene by utilizing blue algae, and the method comprises the following steps of: (1) expressing an isoprene synthetase coding gene derived from blue gum and an isoprene synthetase coding gene with an optimized codon in the blue algae; (2) applying the promotor of a cpc operon to express a mediate terpenoid synthetase coding gene contained in the blue algae; (3) enhancing the expression of an prenyl pyrophosphate isomerase coding gene; (4) expressing a fusion protein which contains a prenyl pyrophosphate isomerase and an isoprene synthetase; and (5) applying an SMUO tag to express a heterologous protein in the blue algae. The method disclosed by the invention can be used for carrying out genetic modification on the blue algae and expressing the isoprene synthetase (IspS) of a plant source in the blue algae, outstandingly increases the output of isoprene produced through the gene engineering blue algae through multiple methods, greatly widens the application range of the gene engineering blue algae by applying research results to express other heterologous proteins in the gene engineering blue algae and has wide industrial application prospect.

The invention discloses a blue green algae growth inhibitor which comprises the following components by weight: 10%-25% of plant extract, 1.5%-5% of a blue green algae complexing agent, 1.5%-3% of blue green algae growth inhibin, 20%-35% of a synergist, 1%-10% of rare earth, 30%-40% of diatom forming soil, 2.5%-8.7% of a bio-enzyme microbial inoculums and 0.5%-10% of humic acid powder. The blue green algae growth inhibitor can be used for killing blue green algae in a long time, and can deprive compulsively blue green algae cell thylakoid pseudo telescopic function for solidifying, sinking, rigidifying and dying of blue green algae cells, eliminating of water bloom and purifying of water quality. The blue green algae growth inhibitor can effectively kill spirogyra, hydrodictyon, chaetophora and bottleneck algae and other mud moss filamentous capsules in an aquaculture pond, and can eliminate a large number of algae attached to meshes in cage culture, and after biological algae removal, beneficial algae and beneficial microorganisms are recultivated to add nutrition balance elements for reactivating the water quality.

The invention discloses an ecological polyculture method of river cabs, freshwater shrimps and rhodeus sinensis, wherein the river cabs, the freshwater shrimps and the rhodeus sinensis are cultured in a mixed mode. The method includes the steps that stocking of crab species is conducted in the middle of March, the specification of young crabs ranges from 40 per jin to 50 per jin, and the stocking density ranges from 600 per mu to 800 per mu; the freshwater shrimps serve as shrimp species, a mode of mixed breeding for two times in one year is adopted, the first breeding is conducted in the middle of February, the stocking specification is that the size of the fresh shrimps ranges from 2 cm to 3 cm, the stocking density ranges from 15 kg / mu to 25 kg / mu, the second breeding is conducted in the end of June, the stocking specification is that the size of the fresh shrimps ranges from 0.7 cm to 1 cm, and the stocking density ranges from 50,000 per mu to 60,000 per mu; stocking of the rhodeus sinensis is conducted in February, the stocking density ranges from 350 per mu to 450 per mu, the male-female ratio ranges from 1:1.1 to 1:1.2, and meanwhile matched stocking of 200-300 anodonta woodianas is conducted. According to the method, the ecological complementarity of the river cabs, the freshwater shrimps and the rhodeus sinensis is adopted to conduct polyculture, the river cabs are mainly bred, the rhodeus sinensis is mainly used for removing residual feeds, the baits are fully utilized, the algae are the objects devoured by the river cabs and the freshwater shrimps, and the rhodeus sinensis is bred in a mixed mode to control the blue-green algae outbreak in the river cab pool.

The invention discloses a compound microorganism controlling cyanobacterial bloom. The compound microorganism comprises components in parts by weight as follows: 10-30 parts of bacillus laterosporus and 10-30 parts of bacillus amyloliquefaciens. The compound microorganism can effectively degrade organic pollutants in water, removes nitrogen and phosphorus, controls and reduces the cyanobacterial bloom, and is free of side effects on the environment and humans. The invention further discloses a preparation method and an application of the compound microorganism.

The invention discloses a cultivation method of polyculture of California weever and yellow-head catfish, which belongs to the technical field of aquaculture, and relates to biological and ecological habits of California weever, yellow-head catfish, silver carp and spotted silver carp, in particular to a 5-30-mu and 1.8-2.8-meter deep pond chosen for the polyculture of the California weevers and the yellow-head catfish and technologies and methods such as a strict preliminary processing, reasonable fish seeds stocking time and density, the mixed feeding of iced fresh fish and extruded feeds, the water quality management, the disease control, the fishing and harvest and the like. The California weevers do not have strong feeding desire for fish blocks and fish mince sink to the bottom of the pond so that the rot of residues of the iced fresh fish is caused with water quality rotten and bacteria breeding. In the California weevers cultivation pond, the yellow-head catfish are polycultured for feeding food residues, the silver carps are polycultured for controlling the generation of blue algae, and the spotted silver carps are polycultured for controlling the generation of plankton, thereby the balance and stability of cultivation eco-system is maintained in the pond, substance and energy flow smoothly in the system, the water quality environment is good, the constitution of cultivated aquatic animals is healthy, the possibility of disease occurring is reduced, the feed coefficient is lowered, and the culture benefit is increased. The method is feasible in technology, which is pioneering in the domestic California weever cultivation field, and is convenient to be applied for cultivation enterprises and aquatic farmers, thereby owning broad prospects.

The invention discloses a method for formation of bioflocs by cyanobacterial bloom. The method includes: acquiring cyanobacterial bloom containing in-situ water from cyanobacterial bloom occurred water and performing proper concentration to keep the total nitrogen concentration of the concentrated cyanobacterial bloom system at 40-150mg / L, the dissolved total organic carbon concentration at 40-150mg / L, and the ratio (mass ratio) of total nitrogen to total phosphorus at 3:1-6:1; maintaining aeration disturbance of the concentrated cyanobacteria bloom at 22-38DEG C for 20-25 days so as to see formed bioflocs, in which a lot of navicula, Stauroneis and other diatoms plants appear. The method can transform cyanobacterial bloom into heterotrophic bacteria preponderant bioflocs, can promote harmless treatment and utilization of nitrogen in water and accelerate conversion of water nutrients, can be directly used for treatment of cyanobacterial bloom, and the formed bioflocs can be directly used for aquaculture or sewage purification.

The invention discloses an alga lysing / algal toxin degradation double-effect engineering strain Y1 and a construction method thereof, belonging to the field of microbiology for treatment of blue-green algae. Fusion is performed on parent strains with alga lysing and algal toxin degradation functions by utilizing a protoplast fusion technology to construct the double-effect engineering strain with excellent traits of parents, namely Bacillussphaericus with the collection number of CGMCCNO. 7519. The alga lysing and the derived algal toxin pollution problem are synchronously solved. The protoplast fusion rate can achieve 31.97% under optimal conditions, and the double-effect engineering strain has certain guide effects on solving the problems of blue-green alga bloom and the derived MC-LR (microcystin-LR) pollution and constructing the engineering strain integrating the MC-LR degradation function and the alga lysing property into a whole by performing a protoplast fusion technology, thereby providing reference for achieving the effect on treating both the root course and the symptoms for solving the large-area blue-green alga bloom problem.

The invention belongs to the field of water purification and relates to a bacillus pumilus with efficient alga-lysing activity and application thereof. The bacillus pumilus NMCC46 with efficient alga-lysing activity is preserved in the China General Microbiological Culture Collection Center with the preservation No. as CGMCC No 3378 and the preservation date as Nov. 2, 2009. The bacillus pumilus NMCC46 has a very strong restraining effect to the growth and reproduction of microcystis aeruginosa, and has the algal inhibiting rate of 97.45% after 7 days. The fermentation supernate of the bacillus pumilus NMCC46 has an excellent alga-lysing effect at the final concentration of 0.5%. Alga-lysing active ingredients generated by the bacillus pumilus have excellent heat resistance and pH stability. Therefore, the bacillus pumilus NMCC46 can be used for controlling cyanobacterial bloom.

The invention discloses an aga lysing biological preparation and an application thereof. The preparation is prepared through mixing Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, Streptococcus faecalis, photosynthetic bacteria, Sphingomonas sp., vitamin C, vitamin E and lactose. The above product has good stability and high efficiency, and consumes an extremely low amount of oxygen and rapidly degrades microcystis and other pollutants in culture water in the application process in order to effectively improve the environment of the water. The preparation is nontoxic and harmless to environment, people and animals, has no residuals, can substantially improve and purify the environment and the culture body and effectively avoid bursting of cyanobacterial bloom, and also can improve the immunity of aquatic product animals and reduce the incidence of diseases to make the ecologic system of the whole culture water tend to balance and go well.