Alga lysing/algal toxin degradation double-effect engineering strain Y1 and construction method thereof

Abstract

The invention discloses an alga lysing/algal toxin degradation double-effect engineering strain Y1 and a construction method thereof, belonging to the field of microbiology for treatment of blue-green algae. Fusion is performed on parent strains with alga lysing and algal toxin degradation functions by utilizing a protoplast fusion technology to construct the double-effect engineering strain with excellent traits of parents, namely Bacillussphaericus with the collection number of CGMCCNO. 7519. The alga lysing and the derived algal toxin pollution problem are synchronously solved. The protoplast fusion rate can achieve 31.97% under optimal conditions, and the double-effect engineering strain has certain guide effects on solving the problems of blue-green alga bloom and the derived MC-LR (microcystin-LR) pollution and constructing the engineering strain integrating the MC-LR degradation function and the alga lysing property into a whole by performing a protoplast fusion technology, thereby providing reference for achieving the effect on treating both the root course and the symptoms for solving the large-area blue-green alga bloom problem.

Description

technical field [0001] The invention belongs to the field of microbiology of cyanobacteria treatment, and in particular relates to a method for constructing alginolytic / algal toxin-degrading double-effect engineering bacteria. Background technique [0002] Excessive N, P and other substances enter the water environment, and the nutrients received by the water body exceed its own purification capacity, resulting in the excessive reproduction of cyanobacteria and other phytoplankton, and the accumulation of buoyant planktonic cyanobacteria on the surface of the water body, resulting in the phenomenon of "bloom". In addition, certain cyanobacteria, such as Microcystis aeruginosa, produce a large amount of algal toxins stored in the cells during the reproduction process, and are released with the death of the algal cells, and exist in the water in a soluble state, which seriously threatens the health of animals and humans. . There are more than 80 types of algae toxins that hav...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the pollution problem of cyanobacteria bloom and its derived algal toxins. In view of the fact that algae-dissolving bacteria dissolve the algae cells, the intracellular algae toxins will be released. The living habits of the strains are different, and the requirements for the environment are inconsistent. It is difficult to construct a complex flora that can not only dissolve algae but also solve the pollution problem of algal toxins derived from it, and make it adapt to the environment.

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