12 results about "Toxin degradation" patented technology

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of fumonisins degrading enzyme by forming disulfide bonds. The thermal stability of the fumonisins degrading enzyme FumD mutant provided by the invention is obviously improved compared with that of wild type fumonisins degrading enzyme. The fumonisins enzyme is mainly applied to the industries of livestock feed processing, food production and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins degrading enzyme mutant with improved thermal stability is beneficial to keeping the activity of the enzyme at a higher temperature, adapts to a higher-temperature production environment, reduces the use amount and replacement frequency of the enzyme, and is convenient to transport and store due to the improvement of the stability, so that the investment of industrial cost can be reduced.

The invention discloses an intelligent quintuplet cooperative plasma self-purification robot long-term storage system and a self-adaptive disinfection control method. According to the system, cold plasma, ozone, chlorine dioxide, self-generated nitric oxide and a charge self-purification jump effect are fused, and a dynamic self-purification system with an electric field-gas field-chemical field-intelligent field-thermal field five-dimensional synergistic energy field is constructed. The robot adopts a three-section structural layout and a multi-mode self-adaptive propulsion mechanism, environmental parameters are sensed through AI, discharge power and oxidant release are intelligently regulated and controlled, and precise sterilization, toxicity reduction and mildew prevention in a multi-medium environment are achieved. The system can generate various high-energy active species containing. OH, O, NO, ClO, ONOO and the like, chain splitting decomposition and oxidation mineralization are carried out on mold spores, bacterial cells, aflatoxin and the like, the sterilization rate reaches 99.995%, and the toxin degradation rate exceeds 98%. The system has the characteristics of no residue, low energy consumption, long-acting self-maintenance and the like, is suitable for multiple scenes of grain storage, traditional Chinese medicinal materials, cold chains, farmland, public health and the like, and realizes an intelligent, continuous and chemical-additive-free green storage and dynamic purification system.

This invention belongs to the field of nanomaterials and food packaging, specifically relating to a nanocomposite packaging film with dual functions of degrading toxins and antibacterial properties, and its preparation method. This invention provides a dual-functional composite film for degrading and inhibiting the growth of mycotoxins (aflatoxin B1), and its preparation method. Prussian blue nanoparticles are loaded onto a chitosan-gelatin composite film to prepare a nanomaterial-based dual-functional composite film that not only degrades mycotoxins and kills bacteria, but also allows for timely recycling, further protecting the nutritional components of food.

The growth of algae can be controlled in water by exposing water containing algae to agitation, for example shear stress and an electrical field. The water may further be detoxified of the microcystins produced by algae by treating the water with a microcystin-degrading bacterium, such as Sphingomonas sp. together with an electric field, with the application of the electric field causing a major acceleration of the toxin's degradation.

The application provides a bacillus natto and application thereof in preparation of functional feed for Chinese mitten crab breeding, and belongs to the technical field of aquaculture. Bacillus natto HQ-1 has been preserved in China Center for Type Culture Collection on August 11, 2025, and the preservation number is CCTCC No. M 20251811. Research shows that the bacillus natto has a water product pathogenic bacteria inhibition effect, a toxin degradation capacity and a water body environment purification function which are significantly improved compared with the same strain. The functional feed prepared by fermentation of the strain contains bioactive ingredients such as probiotic live bacteria, free amino acids, antibacterial lipopeptides and polyglutamic acid, can improve the nutritional form of the feed, improve the body weight, disease resistance and feeding rate of the Chinese mitten crab, and help to reduce the content of harmful substances in the aquaculture water body and improve the water body environmental quality. The application provides a new technical approach for green, antibiotic-free and sustainable breeding of the Chinese mitten crab.

The invention discloses a mycotoxin degrading bacterial agent and application thereof in traditional Chinese medicine storage. According to the microbial agent, bacillus coagulans is used as a core degradation component of ochratoxin, tea residues are used as a carrier, microbial cellulose is generated through kombucha mother fermentation to form a nanometer net-shaped structure, meanwhile, rich pores are formed in the tea residues, an efficient adsorption interface and a diffusion channel are provided for OTA, and an ideal colonization microenvironment is provided for bacillus coagulans. Quercetin can inhibit the synthesis of ochratoxin, and forms a synergistic antibacterial effect with a kombucha fermentation product and bacillus coagulans, so that fungal pollution is doubly controlled from the source and in the process. The microbial agent carrier is stable in structure, can realize sustained release of quercetin and continuous biodegradation of thalli, is natural in raw materials, and is suitable for prevention and control of mycotoxin in a traditional Chinese medicine storage process.

The invention provides a novel biological enzyme capable of effectively degrading algal toxin, a gene, an expression vector, an expression strain, a biosynthesis method and application, and relates to the technical field of biology, the field of environmental governance and the field of food safety. The invention specifically discloses an amino acid sequence and a gene sequence of a novel biological enzyme capable of effectively degrading algal toxin, a preparation method of the biological enzyme and application of the biological enzyme in the aspects of algal toxin degradation and blue-green algae treatment. The invention further discloses application of the biological enzyme to treatment of a mixture containing algal toxin. Therefore, the novel biological enzyme for degrading the algal toxin can be widely applied to agriculture, fishery (including aquaculture and the like) production, river and lake treatment and ecological restoration, detoxification treatment of algal source products polluted by the algal toxin, and detoxification treatment of feeds, fertilizers and other products polluted by the algal toxin; the method is applied to water treatment of rivers, lakes, reservoirs, water sources and the like with algal toxin pollution.

The present invention belongs to the technical field of toxin degradation, and specifically relates to the use of Saccharomyces cerevisiae Hansen for degrading patulin; the steps are as follows: First, Saccharomyces cerevisiae Hansen is inoculated into NYDB medium. After culturing and activation, the bacterial liquid is spread on NYDA medium; then, single colonies are selected and inoculated into NYDB medium. After culturing, the cells are obtained by centrifugation. After washing with sterile distilled water, they are diluted into a bacterial suspension of 1×10<supgt;8< / supgt; cells / mL and inoculated into NYDB medium for culture. A patulin standard solution is added to the NYDB medium, and its initial concentration is adjusted to 10 μg / mL. Under light-shielded conditions, shake culture is carried out to achieve the use of degrading patulin; the results show that the degradation rate is close to 50% at 12 h, and complete degradation is achieved after 30 h, and patulin can be degraded efficiently and rapidly; moreover, the present invention is safe, environmentally friendly, harmless to humans, and has broad application prospects.

A degrading enzyme for vomitoxin and its encoding gene, preparation method and application, belonging to the field of microorganism and genetic engineering technology. In order to solve the technical problem that the technology of biodegradation of vomitoxin mostly depends on a mild operating environment, the present invention provides a degrading enzyme for vomitoxin with an amino acid sequence as shown in SEQ ID NO.1, as well as a gene encoding the above-mentioned degrading enzyme for vomitoxin, a recombinant vector and a recombinant bacterium carrying the gene, and a specific method for preparing the degrading enzyme for vomitoxin. In addition, the present invention uses the enzyme for the degradation of vomitoxin, and it is found that it can play a degradation role under the conditions of 30-70°C and pH 2-9. The enzyme does not depend on a mild operating environment and has broad application prospects in the degradation of vomitoxin.

This invention discloses a strain of *Trichoderma viride* and its application in antibacterial and toxin degradation, belonging to the field of microbial technology. The *Trichoderma viride* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41619. The *Trichoderma viride* strain exhibits a significant inhibitory effect on the growth and infection of *Aspergillus flavus*, with an inhibitory effect superior to 4000 times dilution of tebuconazole. This *Trichoderma viride* strain can produce enzymes that degrade the cell walls of pathogenic fungi. Fungal cell walls are composed of cellulose, glucan, chitin, and glycoproteins. The chitinase and cellulase produced by *Trichoderma viride* can degrade the fungal cell walls, thereby inhibiting the growth, development, and infection of *Aspergillus flavus*. Furthermore, this invention also experimentally demonstrates that *Trichoderma viride* has a highly efficient degradation effect on aflatoxin and fumonisin.

The present invention discloses a degradation system for reducing vomitoxin in wheat, relates to the technical field of toxin degradation, and solves the technical problem that in a specific degradation process, when the toxin or enzyme usage still remains in the unified degradation, the operator is unaware of the problem. The wheat is screened in advance to confirm the finished wheat product, and then the toxin degradation of the finished wheat product is carried out. According to the toxin amount, the enzyme amount value used is confirmed. Subsequently, the specific dissolution process is divided into three groups of stages, each group of stages corresponds to a different subdivision value, and according to the original enzyme amount value, it is also divided into three groups of subdivision values. Subsequently, according to the confirmed subdivision value, the enzyme usage of different stages is confirmed, and they are sequentially prepared to dissolve the vomitoxin in each stage, thereby improving the vomitoxin degradation effect of the entire wheat product.

The invention discloses a screening method of magnaporthe oryzae toxin degrading bacteria, and relates to the technical field of microbiological screening, the method comprises the following specific steps: sample collection and strain preliminary separation: collecting a plurality of samples in different rice field environments, and obtaining pure culture strains through a dilution coating method or a tissue isolation method through multiple times of streak separation; primary screening and HPLC secondary screening based on a culture medium: preparing a culture medium primary screening strain by taking the magnaporthe oryzae toxin as a unique carbon and nitrogen source, through the culture medium primary screening by taking the magnaporthe oryzae toxin as the unique carbon and nitrogen source, potential degradation strains can be rapidly enriched from numerous microorganisms, a large amount of invalid screening is avoided, and the degradation capability of the strains can be accurately determined through HPLC secondary screening; according to the method, efficient degradation strains are quickly locked, target strains are further screened by utilizing the infection relation between bacteriophages and germs through phage-mediated screening, the overall screening efficiency is remarkably improved, the screening period is greatly shortened, and the strains with the capacity of efficiently degrading the pyricularia oryzae toxins are obtained more quickly.