A strain domestication method for improving the degradation efficiency of mycotoxin-degrading bacteria

A technology of mycotoxin and degradation efficiency, applied in the field of microorganisms, can solve the problem of losing the excellent characteristics of wild strains of mycotoxin-degrading bacteria, and achieve the effect of simple method and short domestication time

Active Publication Date: 2021-07-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the problem in the prior art that the excellent characteristics of wild strains of mycotoxin-degrading bacteria are easily lost in the passage, and to provide a strain domestication method that improves the degradation efficiency of mycotoxin-degrading bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Preparation of Bacillus subtilis seed solution

[0049] Take 1mL of Bacillus subtilis ANSB060 (the concentration of live bacteria is 10 9 CFU / mL, at this time, the degradation efficiency of Bacillus subtilis ANSB060 to AFB1 is 73%, without induction, the degradation rate of Bacillus subtilis ANSB060 after 3 generations has decreased to varying degrees), inoculated in 50mL medium for shake flask fermentation For cultivation, the fermentation temperature is 37° C., the pH value is 7.0, the rotational speed is 180 r / min, and the fermentation time is 20 hours to obtain seed liquid.

[0050] Among them, the shake flask fermentation medium is composed of the following components: tryptone 8g, yeast extract 1.5g, glucose 1.5g, beef extract 2g, sodium chloride 3g, disodium hydrogen phosphate 2.5g, magnesium sulfate heptahydrate 0.5g , Distilled water to 800mL, pH value is 7.0.

[0051] After the fermentation, the seed solution was stored in a refrigerator at 4°C for later ...

Embodiment 2

[0064] 1. Preparation of Bacillus subtilis seed solution

[0065] Take 1mL of Bacillus subtilis ANSB01G (the concentration of live bacteria is 10 9 CFU / mL, at this time, the degradation efficiency of Bacillus subtilis ANSB01G to ZEN is 82%, without induction, the degradation rate of Bacillus subtilis ANSB01G after 3 generations has decreased to varying degrees), inoculated in 50mL medium for shake flask fermentation For cultivation, the fermentation temperature is 37° C., the pH value is 7.0, the rotational speed is 180 r / min, and the fermentation time is 20 hours to obtain seed liquid.

[0066] Wherein, the composition of the shake flask fermentation medium is shown in Example 1.

[0067] After the fermentation, the seed solution was stored in a refrigerator at 4°C for later use.

[0068] 2. Induction culture of gradient toxin concentration medium

[0069] Dip the Bacillus subtilis ANSB01G seed liquid obtained in 1, streak it on the LB solid medium with a toxin content of ...

Embodiment 3

[0078] 1. Preparation of Bacillus subtilis seed solution

[0079] Take 1mL of Bacillus subtilis ANSB471 (the live bacteria concentration is 10 9 CFU / mL, the degradation efficiency of Bacillus subtilis ANSB471 to vomitoxin is 83% at this time, without induction, the degradation rate of Bacillus subtilis ANSB471 after 3 generations has different degrees of reduction), inoculated in 50mL medium for shaking flask For fermentation culture, the fermentation temperature is 37° C., the pH value is 7.0, the rotational speed is 180 r / min, and the fermentation time is 20 hours to obtain seed liquid.

[0080] Wherein, the composition of the shake flask fermentation medium is shown in Example 1.

[0081] After the fermentation, the seed solution was stored in a refrigerator at 4°C for later use.

[0082] 2. Induction culture of gradient toxin concentration medium

[0083] Dip the Bacillus subtilis ANSB471 seed liquid obtained in 1, streak it on the LB solid medium with a toxin content o...

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Abstract

The invention discloses a strain domestication method for improving the degradation efficiency of mycotoxin-degrading bacteria in the technical field of microorganisms. The method includes the following steps: activating the degrading bacteria; cultured in culture medium. The strain domestication method for improving the degradation efficiency of mycotoxin-degrading bacteria provided by the present invention does not gradually reduce the mycotoxin degradation efficiency of the degrading bacteria in the process of subculture, but induces it through gradient toxin concentration medium, which instead increases the degradation efficiency of the degrading bacteria. Degradation efficiency, according to the method of the present invention, the mycotoxin degradation efficiency can be adjusted up to 95%. In addition, the method of the invention is simple and effective, has short acclimation time and is suitable for mass production.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain domestication method for improving the degradation efficiency of mycotoxin-degrading bacteria. Background technique [0002] With the general concern of consumers on food hygiene and safety and the deepening of research on mycotoxins by researchers, the contamination of mycotoxins in raw materials and feeds has increasingly attracted the attention of the animal nutrition and feed industries. There are currently more than 300 known mycotoxins, among which aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON) are relatively harmful to humans and animals. AFB1 poisoning mainly manifests as damage to the liver, and reduces the immunity of animals. The clinical manifestations are digestive system dysfunction, decreased feed utilization, and anemia. ZEN has estrogen-like effects and can irreversibly bind to estrogen receptors in the uterus, thereby affecting ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12R1/125
CPCC12N1/36
Inventor 马秋刚赵丽红计成张建云李美玲郑瑞
Owner CHINA AGRI UNIV
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