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A method for Lactobacillus acidophilus to degrade mycotoxins in animals

A technology of Lactobacillus acidophilus and mycotoxins, applied in Lactobacillus, animal feed, animal feed, etc., can solve the problems of economic loss in aquaculture, reduction of muscle quality, and limited types of toxins degraded by a single species of bacteria

Active Publication Date: 2020-01-07
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

T-2 toxin, aflatoxin B 1 (AFB 1 ), deoxynivalenol (DON) and ochratoxin (OTA) are toxic metabolites produced by mold growth, which are common mycotoxins naturally occurring in prawn feed and fish feed. They are highly toxic, difficult to remove, and stable in nature. The occurrence of fish diseases and the decline in production performance and muscle quality have caused huge economic losses to the aquaculture industry and hindered the development of the aquaculture industry. At the same time, fish and shrimp products containing mycotoxins directly or indirectly endanger human life, health and safety through the transmission of food chains , therefore, effective control of its harm is an imminent hot issue
[0003] Lactic acid bacteria, as probiotics, are widely used in various product applications. At present, there are many reports on the degradation of mycotoxins in vitro by lactic acid bacteria, but the types of toxins degraded by single bacteria are limited, and the effect is not good. In addition, they cannot play a role in animals. , cannot play the role of degrading mycotoxins accumulated in animals

Method used

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  • A method for Lactobacillus acidophilus to degrade mycotoxins in animals
  • A method for Lactobacillus acidophilus to degrade mycotoxins in animals
  • A method for Lactobacillus acidophilus to degrade mycotoxins in animals

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Embodiment 1

[0022] (1) Toxicity test on shrimp and tilapia

[0023] Randomly select Penaeus vannamei with healthy physique and uniform individual size (5±0.5g per tail), and randomly distribute them to rectangular hard plastic breeding boxes (volume 40×30×12cm 3 ), use an inflatable culture system to maintain seawater dissolved oxygen DO>6mg / L, pH 6.5-7.5; water temperature 24-28°C; salinity 9.18-11.99%. Change 1 / 3 volume of water every day. Using natural light, feed 5% of the body weight of Penaeus vannamei every day, divided into three times a day, once at 9:00 am, once at 3:00 pm, and once at 9:00 pm. The daily feed-to-feed ratio is 2:3: 5.

[0024] Randomly select tilapias with healthy body and uniform individual size (fish weight 10±05g), and randomly distribute them to rectangular hard plastic breeding boxes (volume 40×30×12cm) according to 30 fish in each group (set 3 parallels). 3 ), water temperature: 25-28 ℃, dissolved oxygen concentration >5 mg / L, pH 7.8-8.2, ammonia nitroge...

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Abstract

The invention belongs to the technical field of toxin degradation, and discloses the application of Lactobacillus acidophilus in degrading mycotoxins in animals, and the mycotoxins are T-2 toxin and aflatoxin B 1 , vomitoxin and ochratoxin; evenly spray the Lactobacillus acidophilus bacteria liquid into the animal feed, and after ten days feed the feed containing lactic acid bacteria and feed the feed containing T-2 toxin, (AFB 1 ), deoxynivalenol (DON), ochratoxin (OTA) toxins in Penaeus vannamei and tilapia, the mycotoxin residues in shrimp and tilapia muscles were significantly lower than those in the control group, among which T‑2 in shrimp muscle decreased by 63.86±1.32, AFB1 decreased by 53.04±0.97%, DON decreased by 70.74±1.47%, and OTA decreased by 70.24±1.22%. Tilapia muscle T‑2 decreased by 54.93±2.52%, AFB1 decreased by 42.18±1.97%, DON decreased by 48.75±0.93%, OTA decreased by 32.79±1.81%; the present invention makes Lactobacillus acidophilus into a feed additive to achieve degradation Mycotoxins accumulated in animals have practical application value.

Description

technical field [0001] The invention relates to the technical field of toxin degradation, in particular to a method for degrading mycotoxins in animals by using Lactobacillus acidophilus. Background technique [0002] Mycotoxins have toxic effects on humans and animals. At present, there are more than 200 kinds of mycotoxins known, and quite a few of them have strong mutagenic, carcinogenic and teratogenic effects. Known toxin-producing fungi mainly include: Aspergillus, Penicillium, Fusarium and so on. T-2 toxin, aflatoxin B 1 (AFB 1 ), deoxynivalenol (DON) and ochratoxin (OTA) are toxic metabolites produced by mold growth. They are common mycotoxins naturally occurring in shrimp feed and fish feed. They are highly toxic, difficult to remove, and stable in nature. They can cause shrimp and The occurrence of fish diseases and the decline in production performance and muscle quality have caused huge economic losses to the aquaculture industry and hindered the development o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K50/80A23K10/18
CPCA01K61/00A23V2400/113Y02A40/81
Inventor 王雅玲孙力军周浪花刘颖
Owner GUANGDONG OCEAN UNIVERSITY
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