14 results about "Pentachlorophenol" patented technology

The invention discloses an analysis method for simultaneously determining residues of telolenol, pentnitrophenol, pentachlorophenol and poisonous phenol in grease. The method comprises the following specific steps: (1) dissolving a grease sample with dichloromethane, carrying out vortex uniform mixing and centrifugation with a sodium hydroxide solution, and taking supernatant; (2) adding formic acid, anhydrous magnesium sulfate, sodium chloride and acetonitrile into the supernatant, carrying out vortex centrifugation, taking the supernatant, freezing and filtering; (3) adding anhydrous magnesium sulfate, a 2% triethylamine solution, multi-walled carbon nanotubes and C18 powder into the filtrate for purification, and filtering after centrifugation to obtain a to-be-detected solution; (4) preparing a matrix blank solution from the negative blank grease through the same pretreatment, and preparing a standard solution with gradient concentration of 1-20ng / mL; and (5) performing quantitative analysis by adopting a matrix standard curve external standard method through an LC-MS / MS detection system. The method has the advantages of economy, simplicity, convenience, high accuracy and high sensitivity, is easy to popularize and use, and can meet the high-throughput detection requirement of phenolic pesticide residues in a complex grease matrix.

This invention relates to a hybridoma cell line secreting a monoclonal antibody against pendimethalin and its application, belonging to the field of immunoassay. The hybridoma cell line secreting the pendimethalin monoclonal antibody of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46744. The pendimethalin monoclonal antibody secreted by this hybridoma cell line is used for the analysis and detection of pendimethalin residues in food safety testing. The pendimethalin monoclonal antibody cell line obtained by this invention can be used for immunoassay detection, exhibiting good detection sensitivity and specificity (IC50) for pendimethalin. 50 The value was 1.87 ng / mL.

The invention belongs to the technical field of biology, and particularly relates to a pentachloronitrobenzene rapid detection method based on enzyme catalysis reaction, which comprises the following steps: converting pentachloronitrobenzene into pentachlorophenol and nitrate radicals through an enzyme system consisting of iron thioflavin protein reductase, ferredoxin and nitrobenzene dioxygenase; and then nitrate radicals are specifically detected by a Griess reagent method so as to indirectly reflect quintozene. The detection method disclosed by the invention is good in specificity, short in detection time and remarkable in visual detection effect, and can be used for on-site rapid detection of quintozene.

This invention discloses a rapid method for detecting pentachlorophenol residues in meat products. The method involves first homogenizing fresh meat products, then extracting them with an acetonitrile solution containing an isotopic internal standard. After purification using a purification column, the residues are detected and calculated using LC-MS / MS. Furthermore, by preparing an adsorbent material containing specific porous materials, the extraction and purification steps are optimized, improving detection efficiency and accuracy. This method, through optimizing the adsorbent structure and detection process, effectively solves the problems of cumbersome pretreatment and significant matrix interference in traditional methods. It offers advantages such as speed, accuracy, and high stability, making it suitable for the efficient detection of pentachlorophenol residues in meat products and providing a reliable technical means to ensure food safety.

The application discloses a preparation method of a molecular imprinting electrochemical sensor for detecting sodium pentachlorophenol and detection application thereof, and is characterized by comprising the following steps: after ultrasonic dispersion of a sodium pentachlorophenol molecular imprinting polymer suspension, the suspension is drop-coated on the surface of a treated glassy carbon electrode, and the molecular imprinting electrochemical sensor is obtained by standing and drying at room temperature; and a sodium pentachlorophenol detection method is also provided and comprises the following steps: the sensor is placed in a phosphate buffer solution containing Ru(bpy)3 2+ TPrA is polarized, and an initial ECL signal intensity I is recorded; after a to-be-detected PCP-Na solution is added, the ECL intensity I0 is determined by standing and reacting, the ECL signal change value ΔI=I-I0 is taken as a response index, the PCP-Na concentration in the to-be-detected PCP-Na solution is calculated according to the linear relationship between the PCP-Na solution concentration and ΔI, and the method has the advantages of good selectivity and stability, high sensitivity and accuracy.

The invention discloses a method for analyzing and determining 4-nonylphenol, bisphenol A and pentachlorophenol in water, which comprises the following steps of: eluting impurities in a solid-phase extraction column by using a reagent, adding a water sample into the solid-phase extraction column, leaving a compound to be detected in a filler of the solid-phase extraction column, discarding water, eluting the compound to be detected in the filler by using an organic reagent, and detecting the content of 4-nonylphenol, bisphenol A and pentachlorophenol in the solid-phase extraction column. The method comprises the following steps of: adding 4-nonylphenol and bisphenol A into an organic solvent, concentrating, separating through liquid chromatography, detecting pentachlorophenol through an ultraviolet detector, detecting 4-nonylphenol and bisphenol A through a fluorescence detector, and determining nature and quantifying peak area through retention time of an ultraviolet spectrogram and a fluorescence spectrogram. The method overcomes the defect that three traditional compounds need to be independently analyzed and determined, is simple and convenient to operate and easy to implement, does not need expensive materials, can be used for obtaining reagent consumables in situ, saves the manpower and material resource cost, and improves the efficiency of analysis and determination operation.

The application discloses a 3D printing consumable containing pentachlorophenol-silane synergistically modified polylactic acid and a preparation method thereof. The 3D printing consumable containing pentachlorophenol-silane synergistically modified polylactic acid is characterized by comprising TPU, a compatilizer, a dispersing agent, a pigment and pentachlorophenol-silane synergistically modified polylactic acid. The preparation method of the pentachlorophenol-silane synergistically modified polylactic acid comprises the following steps: esterification reaction between carboxyl groups of polylactic acid and hydroxyl groups of pentachlorophenol to obtain pentachlorophenol grafted polylactic acid; and esterification reaction between hydroxyl groups of polylactic acid and carboxyl groups of a silane coupling agent to obtain pentachlorophenol-silane synergistically modified polylactic acid. The application can improve the sterilization performance.

The utility model belongs to soil detection technical field especially for the soil pentachlorophenol determination device based on spectrophotometry, including box and spectrophotometric determinator, the spectrophotometric determinator is fixed in the inside of box, the spectrophotometric determinator includes the casing, the casing is installed with light source, monochromator body, support platform and spectral detection head, one side of spectral detection head is provided with control panel. The utility model discloses through placing the spectrophotometric determinator in the box, when detecting the content of pentachlorophenol in the soil, the fan built-in in the box can forcibly exhaust and discharge volatile gas, forms the negative pressure environment, effectively prevents the toxic gas from leaking, simultaneously, the negative ion generator inside the purification subassembly releases high-energy negative ion, destroys pentachlorophenol molecular structure through oxidation-reduction reaction, reduces its toxicity, and the subsequent double processing of activated carbon honeycomb plate and chemical adsorption layer reduces the possibility of causing the influence to the environment of laboratory and the health of detection personnel.

The invention provides a sodium pentachlorophenate rapid detection kit suitable for bamboo materials and aquatic products, the rapid detection kit is a colloidal gold kit, and the colloidal gold kit comprises a reagent plate, a microporous reagent and a diluent; the diluent is a phosphate composite solution. According to the valve, rapid, efficient and sensitive detection of sodium pentachlorophenate residues in two matrixes is realized, the pretreatment process is simplified, the detection time is shortened, meanwhile, the accuracy and the stability of a detection result are ensured, and the actual requirements of field detection and batch screening are met.

This application relates to the field of analytical testing technology, specifically to a method for detecting pentachlorophenol (PCP) in animal-derived foods. The method includes providing 2g-10g of a sample from an animal-derived food source; alkalizing and extracting the sample using ammonia and acetonitrile to obtain a first mixture; adding a salting-out agent to the first mixture, shaking to disperse, then vertically oscillating for 2-5 minutes, followed by centrifugation at 3000-4000 rpm for 2-5 minutes; taking 2mL of the supernatant, adding it to a purification tube filled with packing material, mixing thoroughly, and filtering using a 0.22µm organic filter membrane to obtain the test solution; and performing LC-MS / MS detection using the test solution. This method optimizes the pretreatment process of the QuEChERS method while ensuring PCP extraction rate, thereby improving the efficiency and reducing the cost of PCP detection in animal-derived foods.

The application provides an anti-interference molecular imprinting sensor and a preparation method thereof and application thereof in detection of pentachlorophenol. The preparation method of the application comprises the following steps: providing an Au NPs / GCE electrode; dropping hydroxyproline helical peptide PHHP on the surface of the Au NPs / GCE electrode, and incubating under infrared lamp irradiation to obtain a polypeptide modified electrode interface; preparing an electrolyte containing a polymer monomer and a PCP molecular imprinting template, and performing electrochemical polymerization on the polypeptide modified electrode interface; washing the surface of the electrode, and performing elution, and finally removing the PCP template molecule to obtain the anti-interference molecular imprinting sensor. The Au NPs coating is helpful to enhance the voltammetry response, and the optimized setting of the MIP layer provides a higher peak change. The prepared electrochemical sensor is used for detecting PCP in water, and has the advantages of high specificity, strong stability and good anti-interference capability.

The invention discloses a method for removing pentachlorophenol in water by using a modified biochar-based immobilized inoculant, the method is characterized in that the modified biochar-based immobilized inoculant is used for degrading pentachlorophenol-containing water, the modified biochar-based immobilized inoculant takes modified biochar as a carrier, PCP degrading bacteria are loaded on the modified biochar, and the modified biochar-based immobilized inoculant is used for degrading the pentachlorophenol-containing water. Wherein the PCP degrading bacteria are obtained by domesticating activated sludge serving as a raw material and pentachlorophenol serving as a carbon source. The method disclosed by the invention has the advantages of simple process, simplicity and convenience in operation, low treatment cost, good removal rate and the like, is relatively high in practical value, has a good application prospect, and is a novel and environment-friendly method for removing pentachlorophenol in water.

The invention discloses a method for rapidly detecting residual quantity of pentachlorophenol in meat products. The method comprises the following steps: homogenizing a fresh meat product, adding an acetonitrile solution containing an isotope internal standard for extraction, purifying through a purification column, and detecting and calculating the residual quantity by using LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry); by preparing an adsorption material containing a specific porous material and optimizing extraction and purification steps, the detection efficiency and accuracy are improved. The method effectively solves the problems of tedious pretreatment, large matrix interference and the like of a traditional method by optimizing the adsorbent structure and the detection process, has the advantages of rapidness, accuracy and high stability, is suitable for efficient detection of pentachlorophenol residues in meat products, and provides a reliable technical means for guaranteeing food safety.

The application discloses a method for degrading halogenated phenolic compounds by using nano high-purity iron oxide activated low-concentration ferrate, and the method comprises the following steps: dissolving potassium ferrate powder in borax or dipotassium hydrogen phosphate buffer to prepare a Fe(VI) solution at normal temperature; and adding the Fe(VI) solution into a boric acid buffer containing halogenated phenolic compounds and high-purity iron oxide, and stirring to carry out degradation reaction. The method for degrading halogenated phenolic compounds by using nano high-purity iron oxide activated low-concentration ferrate realizes the activation of Fe(VI) through nano alpha-Fe2O3, realizes the in-situ generation of iron oxide through nano-sized particles with size effect instead of added iron salt in the role of Fe(VI) solution activation, rapidly degrades trace amounts of pentachlorophenol in water, and simultaneously realizes the recycling of the activator.