The invention belongs to the technical field of treatment of tunnel defects and discloses an automatic operation system for comprehensive tunnel defect treatment. The automatic operation system comprises a vehicle-mounted platform, a guiding device, horizontal rotating mechanisms, mechanical arms, auxiliary sensing devices, concrete construction equipment, mortar injection equipment and high-pressure cleaning equipment. The guiding device comprises a track, guide rails and bases; each mechanical arm comprises a machine body, a large arm body, a small arm body, a hand part and pneumatic paws; each auxiliary sensing device comprises an image sensor and a laser distance measuring instrument which are both mounted on the corresponding hand part; and the concrete construction equipment, the mortar injection equipment and the high-pressure cleaning equipment are all mounted on the vehicle-mounted platform so as to be matched with the mechanical arms for defect treatment of tunnels. The automatic operation system for comprehensive tunnel defect treatment is suitable for defect treatment of the road and highway tunnels and carries operation equipment such as the drilling equipment, the mortar injection equipment, the cleaning equipment and the concrete pouring equipment, so that the automatic operation system can rapidly arrive at the defect parts of the tunnels to comprehensively treat the tunnel defects, and the problems that during manual building of an operation platform in the prior art, much labor and time are consumed, and the efficiency is low are avoided.