The invention relates to a clean operating room with an air cleaning and conditioning system with intelligent adjustment and control. A humiture and pressure difference combination sensor and a control unit are installed in an aseptic clean space; an air conditioning, cleaning and ventilating system comprises a fresh air conditioning unit and a transducer thereof, a cycling cleaning unit and a transducer thereof, a duty fan, an air supply plenum chamber, a high efficiency filter, an exhaust fan and a transducer thereof, an embedded controller and a control panel, wherein the fresh air conditioning unit, the cycling cleaning unit and the air supply plenum chamber are sequentially connected in series through air flues; the high efficiency filter is connected with the plenum chamber into a whole; and the high efficiency filter is arranged at the top of the clean operating room and the air outlet of the high efficiency filter is the input port of cleaned air after adjustment and control at the top of the aseptic clean space. In the invention, a plurality of typical working conditions can be respectively set according to different operation types or working processes, air parameters such as temperature, humidity, air delivery volume, fresh air volume and the like can be preset by the control unit in the aseptic clean space, and the working conditions can be combined for operation according to the actual use load, so the energy saving effect is very obvious.