The invention discloses a
cell sample low-temperature
transmission system based on a
sound wave suspension principle, and aims to solve the problems of
cell damage, low efficiency, poor compatibility with an existing biological sample
library and the like caused by a traditional
mechanical transmission mode. According to the
system, a
standing wave sound field is generated in a
transmission channel through a high-frequency piezoelectric
transducer array (20-40kHz, and the
sound pressure level is greater than or equal to 130dB), so that non-contact suspension of a
cell sample is realized; a
gaseous nitrogen low-temperature environment (-150 DEG C to-196 DEG C) and a
helium doping technology (1-2% by volume) are combined, so that the
cooling rate (gt; 1000 DEG C / min), inhibiting the formation of
ice crystals (volume ratio lt; 0.1%). A phase array cooperative control and AI trajectory prediction
algorithm (CNN-LSTM fusion model) is adopted to drive a sample to be transmitted at a high speed of 50mm / s, and the speed is increased by 5 times compared with that of a traditional method. The
modular design is compatible with an SBS standard interface (such as a 96-well plate) and an
open communication protocol (RS-485 /
CAN bus), and supports seamless docking of mainstream biological sample
library equipment. Experiments show that the
survival rate of cells transmitted by the
system is larger than or equal to 99.1%, the ice
crystal damage rate is smaller than or equal to 0.3%, the
system has full-process
automation and data
traceability, and an innovative solution is provided for high-
throughput and high-activity transmission of biological samples.