Immunochromatography test strip for synchronously detecting mixed pollution of aflatoxin, ochratoxin A and zearalenone, and preparation method

A technology of zearalenone and immunochromatographic test paper, which is applied to measuring devices, analytical materials, instruments, etc., can solve the problems of various mycotoxin pollution and high incidence of mycotoxins
CN103217531AInactive Publication Date: 2013-07-24INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2013-07-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an immunochromatography test strip for synchronously detecting the mixed pollution of aflatoxin, ochratoxin A and zearalenone, and a preparation method. The immunochromatography test strip comprises a paperboard, wherein a water-absorbing pad, a detection pad, a gold label pad and a sample pad are sequentially bonded on one surface of the paperboard from top to bottom, and the adjacent pads are connected at a junction in an overlapping manner; the detection pad takes a nitrocellulose membrane as a base pad, and a transversal quality control line and detection lines are arranged on the detection pad; three detection lines are located below the quality control line, distributed at intervals, and coated with OTA-BSA (ochratoxin A-bovine serum albumin), ZEA-BSA (zearalenone A-bovine serum albumin) and AFB1-BSA (aflatoxin B1-bovine serum albumin) respectively; and the quality control line is coated with a rabbit anti-mouse polyclonal antibody; the gold label pad is transversally spayed with a nanogold labelled anti-aflatoxin universal monoclonal antibody, a nanogold labelled anti-ochratoxin A monoclonal antibody and a nanogold labelled anti-zearalenone monoclonal antibody. The immunochromatography test strip can be used for synchronous detection for aflatoxin, ochratoxin A and zearalenone, as well as is simple and rapid to operate, and high in sensitivity.
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Description

technical field

[0001] The invention provides an immunochromatographic test strip and a preparation method for synchronously detecting mixed pollution of aflatoxin, ochratoxin A and zearalenone. Background technique

[0002] Mycotoxins are metabolites produced by fungi growing in food or feed. If the food and feed are not fully dried at the time of harvest or the temperature or humidity is too high during storage and transportation, it may provide suitable conditions for the growth and reproduction of fungi, thereby causing Mycotoxin contamination. Aflatoxins, ochratoxins and zearalenone are common mycotoxins in grain feed. Aflatoxin is mainly produced by Aspergillus flavus and Aspergillus parasiticus, ochratoxin is mainly produced by various Aspergillus and Penicillium, zearalenone is mainly produced by Fusarium graminearum, three kinds of mycotoxins are mainly produced in grains including wheat, corn, barley , oats, rye, rice and millet, etc., as well as peanuts, beans a...

Claims

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