The invention relates to
Aspergillus tubingensis and a method for producing theabrownins through liquid
fermentation of
Aspergillus tubingensis, and belongs to the technical field of
biology. The producing strain of the invention is
Aspergillus tubingensis KUtea 00, and
Aspergillus tubingensis KUtea 00 is preserved in the China General
Microbiological Culture Collection Center (CGMCC) on December 30th, 2014, the preservation unit address is No.3. No.1 Courtyard, West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.10021. According to the invention,
Aspergillus tubingensis KUtea 00 is inoculated in an
aqueous extract of
Camellia sinensis var. assamica which is pasteurized, after a single
bacterial strain is purely cultured for 4 days in a
shake flask, the content of theabrownins is 6.5g / L, and the volumetric productivity of theabrownins is 5.5 times higher than that of
solid state
fermentation. The
Aspergillus tubingensis and the method for producing theabrownins by the liquid
fermentation of Aspergillus tubingensis disclosed by the invention have the advantages of easy control of production conditions and environment, no limit to tea production seasons,
automation, high efficiency and low cost. According to the invention, the strain is directly grown and reproduced in the
aqueous extract of
Camellia sinensis var, without adding any additional carbon sources and
nitrogen sources, and polyphenols in the
aqueous extract of
Camellia sinensis var can be biologically transformed into
macromolecule water-soluble theabrownins quickly.