'Tabin' aspergillus capable of producing pectase in high yield, and application in production of solid-state fermentation
A technology of Aspergillus tubingensis and solid-state fermentation, which is applied in the direction of hydrolytic enzymes and fungi, and can solve the problems of increased activity of fermented enzymes
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Embodiment 1
[0071] Take a slant strain of Aspergillus tubingensis Moss (Aspergillus tubingensis Moss) CGMCC 0826, add 5ml of sterile water, and wash the spores of Aspergillus tubingensis Moss. Draw spore liquid then, inoculate in the 50g seed culture medium (pH6.0, water content be 50%) that is made up of wheat bran (96%), ammonium sulfate (2.0%) and potassium dihydrogen phosphate (2.0%), After culturing at 30°C for 48 hours, insert 2 kg of solid medium (pH6.8) composed of wheat bran (90%), ammonium sulfate (2.0%), potassium dihydrogen phosphate (2.0%) and orange peel powder (6.3%). , with a water content of 60%). After culturing at 28°C for 58 hours, the pectinase activity in the medium reached 122.4u / g.
Embodiment 2
[0073] Take a slant strain of Aspergillus tubingensis Moss (Aspergillus tubingensis Moss) CGMCC 0826, add 10 ml of sterile water, and wash the spores of Aspergillus tubingensis Moss. Draw spore liquid 5ml then, inoculate in the 50g seed culture medium (pH6.0, water content is 50%) that is made up of wheat bran (96%), ammonium sulfate (2.0%) and potassium dihydrogen phosphate (2.0%), After culturing at 30°C for 48 hours, insert 2 kg of wheat bran (90%), ammonium sulfate (2.0%), potassium dihydrogen phosphate (2.0%), orange peel powder (2.0%) and sugar beet pulp (4.0%). Solid medium (pH6.7, water content 60%). After culturing at 28°C for 60 hours, the pectinase activity in the medium reached 132.2u / g.
Embodiment 3
[0075] Take a slant strain of Aspergillus tubingensis Moss (Aspergillus tubingensis Moss) CGMCC 0826, add 5ml of sterile water, and wash the spores of Aspergillus tubingensis Moss. Draw spore liquid 3ml then, inoculate into the 20g that is made up of wheat bran (90%), ammonium sulfate (2.0%), potassium dihydrogen phosphate (2.0%), orange peel powder (2.0%) and sugar beet pulp (4.0%) In the solid medium (pH 6.8, water content 50%), after the medium was packed in a 250ml Erlenmeyer flask and cultured at 30°C for 62 hours, the pectinase activity in the medium reached 120.5u / g.
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