15 results about "Pectinase activity" patented technology

The invention discloses a fruit and vegetable grafting wound healing nutrient solution containing polysaccharide complex enzyme and a preparation method, belongs to the technical field of agricultural biochemical preparations, and aims to solve the technical problems of low removal efficiency of necrotic tissues at grafting wounds and high risk of microbial infection. According to the technical scheme, the nutrient solution is characterized by being prepared from the following raw material components in parts by weight: 0.3 to 0.8 part of polysaccharide, 1 to 5 parts of cellulase, 0.5 to 2.5 parts of pectinase, 1 to 5 parts of glycerol and 90 to 100 parts of water. Wherein the pH value of the nutrient solution is adjusted to 5.0-6.5 by using a phosphate buffer agent, the polysaccharide is composed of chitosan and sodium alginate, the mass ratio of the chitosan to the sodium alginate is 1: 0.5-1: 2, the activity of cellulase in the nutrient solution is 100-500 U / mL, and the activity of pectinase in the nutrient solution is 50-250 U / mL. The nutrient solution is mainly used for promoting fruit and vegetable grafting wound healing, and application verifies that the nutrient solution can effectively improve wound healing quality and reduce infection risk in the grafting process of fruits and vegetables such as cucumbers, eggplants, peppers, tomatoes, litchis and oranges, and has a good application effect.

In the present invention pectinase enzymes for cleaning compositions and the cleaning compositions comprising them are provided. Additionally, methods of making and using these enzymes, including recombinant polynucleotides encoding them, are provided.

The invention provides the method for fluorescence quantitative determination of the activity of the endo-pectinase, the enzyme activity of the endo-pectinase can be accurately detected by constructing a proper endo-pectinase enzyme activity detection system, the method is green, safe, convenient to operate and low in cost, the defects of the existing detection method can be avoided, and the method can be widely applied to detection of the endo-pectinase activity of the endo-pectinase activity of the endo-pectinase activity of the endo-pectinase activity of the endo-pectinase activity of the endo-pectinase. The application prospect is wide.

The invention discloses an engineering bacterium Lactobacillus plantarum JY002 for fermenting traditional Chinese medicinal materials, the preservation number of the engineering bacterium is CCTCC (China Center For Type Culture Collection) NO: M 2025585, and the preservation date is March 25, 2025. Compared with the existing commercial strain lactobacillus plantarum, the preserved strain has ligninase activity, pectinase activity and alcohol resistance. The saccharomyces cerevisiae NJ01 has better ligninase activity and wine resistance than commercial strain saccharomyces cerevisiae NJ01, can be used as a traditional Chinese medicinal material fermentation engineering bacterium, especially can maintain high ligninase and pectinase activity under the yellow wine processing condition, and can be used for traditional Chinese medicinal material fermentation or wine-processed traditional Chinese medicinal material fermentation.

This invention discloses a strain of Kluyveromyces marxianus and its application in coffee bean degumming technology, belonging to the field of microbiology. The Kluyveromyces marxianus strain CI-06 was deposited on May 9, 2024, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 30564. The CI-06 strain possesses pectinase activity, and the pectinase it produces has high activity, which can be used in the biological degumming process of coffee to shorten degumming time and improve the quality of commercial coffee beans.

The invention discloses a fermentation engineering bacterium Lactobacillus plantarum JY003 for wine-processed traditional Chinese medicine decoction pieces, the preservation number of the fermentation engineering bacterium is CCTCC (China Center For Type Culture Collection) NO: M 20252157, the preservation date is September 29, 2025, and the classification name of the fermentation engineering bacterium is Lactobacillus plantarum JY003. The preserved strain can tolerate alcoholic strength of 10-15%, has both pectinase activity and cellulase activity, can keep high cellulase activity and pectinase activity under the yellow wine processing condition, further promotes plant cell wall degradation, enables active ingredients in cells to be released and dissolved out, can be used as a wine-processed traditional Chinese medicine decoction piece fermentation engineering strain, and has a wide application prospect. The method is used for preparing wine-processed traditional Chinese medicine decoction pieces.

The application discloses a kind of lipopeptide class for preventing and treating walnut rot disease and preparation method thereof, it is related to plant disease control technical field, including the nanocarrier core of lipopeptide class loading;And the responsive material shell of the carrier core outside is covered;Wherein, the responsive material shell can be degraded under the microenvironment of walnut tree disease site, the microenvironment characteristics include pH value is lower than 6.0 and there is pectinase activity.The present application realizes the precise and efficient prevention and treatment of walnut rot disease by constructing the intelligent nanometer drug delivery system of "nuclear shell structure", realizes the targeting and on-demand release of lipopeptide in lesion site using the shell with double response to disease microenvironment, greatly improves drug utilization and prolongs the effective period;Nanocarrier core effectively protects lipopeptide activity, and its 50-200 nanometer optimized particle size and surface modification promote drug delivery and enrichment in tree body.

An arabinose-containing and galactose-containing food composition is disclosed. Said food food composition is liquid or semi-liquid or powdered. It comprises plant proteins, less than 5wt.%free uronicacid by dry weight and a free arabinose to free galactose weight ration of from 1:1.5 to 1:5. A process for preparing such a food composition is also disclosed. A method for selecting an enzyme comprising a pectinase activity suitable for the invention is also disclosed.

The invention relates to acinetobacter calcoaceticus MZ-02 as well as a biological preparation and application thereof, and aims to reduce the content of pectin and total sugar in a cigar fermentation process, improve the physical characteristics and sensory quality of tobacco leaves and comprehensively improve the overall quality of cigars. The acinetobacter calcoaceticus MZ-02 is obtained through screening, and the preservation number of the acinetobacter calcoaceticus MZ-02 is CCTCC NO: M 2025986. The strain is fermented to prepare a biological preparation containing acinetobacter calcoaceticus MZ-02, the pectinase activity is 8.94 U / ml, and after fermentation optimization, the pectinase activity is 15.21 U / ml. When the biological agent containing MZ-02 is applied to cigar tobacco fermentation, compared with a blank group and a control group, the pectin content and the total sugar content in the tobacco leaves can be obviously reduced, the physical characteristics and the sensory quality of the tobacco leaves are improved, and the overall quality of cigars is comprehensively improved. And the process flow is simple and convenient, the fermentation period is relatively short, and the method has relatively good popularization and application values.

The application discloses a Pichia kluyveri strain, a Streptococcus lactis strain and application of co-fermentation of the two strains in coffee green bean degumming, and belongs to the technical field of microbial fermentation. The two strains (K123 and L135) are applied to co-fermentation in coffee green bean degumming, and can also be applied to preparation of coffee biological degumming preparation. When the compounding ratio of the strain compounding bacteria liquid is K123:L135=2:1, the fermentation temperature is 32 DEG C, the concentration is 10 7 CFU / mL, and the fermentation time is 36h, the pectinase activity can reach 982.985u / g, pectin can be effectively decomposed, biological degumming of coffee green beans can be realized, and the application prospect is good.

The invention discloses Pichia kluyveri, Pediococcus acidilactici and application of co-fermentation of the Pichia kluyveri and the Pediococcus acidilactici in degumming of fresh coffee beans, and belongs to the technical field of microbial fermentation, and the two strains (K I 23 and L II 35) are jointly fermented to be applied to degumming of the fresh coffee beans and can also be used for preparing a biological degumming preparation of coffee. When the compounding ratio of a bacterial strain compounded bacterial solution is K I 23: L II 35 = 2: 1, the fermentation temperature is 32 DEG C, the concentration is 107 CFU / mL, and the fermentation time is 36 hours, the pectinase activity can reach 982.985 u / g, pectin can be effectively decomposed, biological degumming of fresh coffee beans is achieved, and good application prospects are achieved.

The present application belongs to the field of enzyme activity determination method, aiming at the problems of existing pectinase activity determination technology, such as complicated operation, low sensitivity, and the problems of existing MBTH method for determining pectinase activity, such as a large amount of precipitate in the chromogenic product solution and the high background value of the chromogenic product, the present application provides a kind of determination method of pectinase activity: a series of concentration gradient galacturonic acid standard solution is prepared by using substrate solution, the precipitate is provided by using alkaline reagent to provide high concentration alkaline environment, and the precipitation of precipitated material is accelerated by using low temperature condition, then the supernatant is obtained by centrifugation, MBTH reaction is carried out, the absorbance value is determined, and the standard corresponding relationship curve is drawn; Pectinase enzymolysis reaction; preparation of enzymolysis blank; the initial speed of the concentration of reducing end group produced by the enzymolysis of the substrate per unit time is determined to calculate the enzyme activity of pectinase. The method is accurate, reliable, good in repeatability, high in sensitivity and stable in chromogenic product.

The invention provides a salt-tolerant bacillus and application thereof, and belongs to the technical field of microorganisms, the salt-tolerant bacillus is named as the salt-tolerant bacillus CC and preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251509. The salt-tolerant bacillus CC provided by the invention is a strain separated from tobacco leaves, and has better colonization ability and environmental adaptability; and meanwhile, the compound enzyme has pectinase producing activity, can reduce pectin to 17.2% in the cigar tobacco leaf fermentation process, remarkably reduces the bitter taste of cigars, can improve the fragrance and flavor of cigars, reduces the generation of unpleasant odor and irritant substances, remarkably improves the sensory quality score of cigars, and improves the overall quality of tobacco leaves.

The invention discloses a tea leaf prototype wall-breaking processing method, which belongs to the technical field of tea leaf processing, and comprises the following steps: S1, raw material pretreatment: selecting complete bud leaf tea leaves, removing impurities, and refrigerating in a low-temperature environment of-5 DEG C to 0 DEG C; then washing the tea leaves with deionized water at 30-40 DEG C for 2-3 times, draining water, and controlling the water content of the tea leaves to be 60-70%; s2, composite wall breaking treatment: sequentially carrying out low-temperature rolling, stepped ultrasonic treatment and directional enzymolysis on the pretreated tea leaves; s3, enzyme inactivation and post-treatment: heating the enzymolysis system to 85-90 DEG C, and keeping the temperature for 10-15 minutes, so that the residual activity of cellulase and pectinase is less than or equal to 1%, thereby completing enzyme inactivation; after the tea leaves are fished out, vacuum freeze drying is performed under the conditions that the vacuum degree is smaller than or equal to 10 Pa and the freezing temperature is minus 40 DEG C to minus 30 DEG C until the water content is 8-12%; the invention discloses a processing method which is uniform in wall breaking, high in effective component retention rate and capable of maintaining the original shape characteristics of tea leaves, and the tea leaves with the wall breaking rate larger than or equal to 85% are screened in three stages according to the particle size of 40 meshes, 60 meshes and 80 meshes.

The present invention relates to isolated polypeptides having pectinase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.