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Complex enzyme capable of degrading cotton seed hull as well as ramie induction based preparation method and application thereof

A technology of compound enzyme and cottonseed husk, which is applied to the fermentation preparation of compound enzyme and its application field, can solve the problems of not achieving the best synergistic treatment effect, very different catalytic conditions, and difficult to master the ratio.

Inactive Publication Date: 2011-02-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the enzyme compounding technology is adopted, due to the different characteristics of the enzymes from different biological sources, the catalytic conditions are very different, and the ratio of various enzymes is difficult to master, so the best synergistic treatment effect cannot be achieved

Method used

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  • Complex enzyme capable of degrading cotton seed hull as well as ramie induction based preparation method and application thereof
  • Complex enzyme capable of degrading cotton seed hull as well as ramie induction based preparation method and application thereof
  • Complex enzyme capable of degrading cotton seed hull as well as ramie induction based preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Seed Culture of Trichoderma reesei

[0068] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N (125g / L sodium citrate dihydrate, 250g / L KH 2 PO 4 , 100g / L NH 4 NO 3 , 10g / LMgSO 4 ·7H 2 O, 250μg / L Biotin, 5g / L CaCl 2 2H 2(2, 5mL / L trace element solution); Wherein, trace element solution contains 50g / L citric acid monohydrate, 50g / L ZnSO 4 ·7H 2 O, 10g / L Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O, 2.5g / L CuSO 4 ·5H 2 O, 0.5g / L MnSO 4 ·H 2 O, 0.5g / L H 3 B0 3 , 0.5g / L Na 2 MoO 4 2H 2 O;

[0069] (2) Trichoderma reesei (T. reesei) Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 36 hours.

[0070] 2. Fermentation of Trichoderma reesei to produce enzymes

[0071] (1) Trichoderma reesei enzyme production medium: 0.1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N, adding 2% ramie powder, pH5.0;

[0072] (2) With 10% inoculum ...

Embodiment 2

[0109] 1. Seed Culture of Trichoderma reesei

[0110] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N;

[0111] (2) Trichoderma reesei Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 36 hours.

[0112] 2. Fermentation of Trichoderma reesei to produce enzymes

[0113] (1) Trichoderma reesei enzyme production medium: 0.5% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N, adding 2% ramie powder, pH5.0;

[0114] (2) With 5% inoculum size, the culture without ramie powder was used as a control, at 30 ° C, 160 r / min conditions were cultivated for 8 days to induce enzyme production;

[0115] (3) The fermented broth supernatant collected by filtration or centrifugation is exactly the compound enzyme liquid for hydrolyzing cottonseed hulls.

[0116] 3. Determination of cellulase activity, see Example 1 for details.

[0117] 4. Determination of...

Embodiment 3

[0123] 1. Seed Culture of Trichoderma reesei

[0124] (1) Trichoderma reesei seed medium: 10g / L glucose, 1g / L peptone, citric acid buffer solution with a final concentration of 0.05M, 0.15g / L Tween 80, 1.4g / L (NH 4 ) 2 SO 4 , 2.0g / L KH 2 PO 4 , 0.3g / L urea, 0.4g / L CaCl 2 2H 2 O, 0.3g / L MgSO 4 ·7H 2 O, 0.005g / L FeSO 4 ·7H 2 O, 0.0016g / L MnSO 4 ·H 2 O, 0.0014g / LZnSO 4 ·7H 2 O, 0.0037g / L CoCl 2 ·6H 2 0 Mandels medium, pH4.8-5.0;

[0125] (2) Trichoderma reesei Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 36 hours.

[0126] 2. Fermentation of Trichoderma reesei to produce enzymes

[0127] (1) Trichoderma reesei enzyme production medium: 10g / L glucose, 1g / L peptone, citric acid buffer solution with a final concentration of 0.05M, 0.15g / L Tween 80, 1.4g / L (NH 4 ) 2 SO 4 , 2.0g / L KH 2 PO 4 , 0.3g / L urea, 0.4g / LCaCl 2 2H 2 O, 0.3g / L MgSO 4 ·7H 2 O, 0.005g / L FeSO 4 ·7H 2 O, 0.0016g / L MnSO 4 ·H 2 O, 0.0014g / L ZnSO...

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Abstract

The invention relates to a complex enzyme capable of degrading a cotton seed hull as well as a ramie induction based preparation method and application thereof. The complex enzyme comprises the components of cellulose, xylanase and pectinase vigour. The preparation method comprises the following steps of: vaccinating a trichoderma reesei seed into a seed culture medium for culturing for 24-48 hours under the conditions of 25-30 DEG C and 120-250r / minute; inoculating to a fermentation culture medium containing 1-10 percent of ramie powder by the inoculum size of 4-10 percent to produce enzymes; further inducing to produce enzymes by adding the ramie powder in the culture process; culturing for 4-8 days under the conditions of 25-30 DEG C and 120-250r / minute; and filtering and centrifuging to obtain rough enzyme liquid. The complex enzyme prepared by the invention has the advantages of higher enzyme activity, safety, environmental protection, simple operation, high controllability, mildreaction condition compared with conventional alkali treatment and favorable application prospect and is more suitable for a complex enzyme system for degrading a cotton seed hull.

Description

technical field [0001] The invention belongs to the field of fermentation preparation and application of compound enzymes in microbial technology, and in particular relates to a compound enzyme capable of degrading cotton seed hulls and its preparation method and application induced by ramie. Background technique [0002] The chemical composition, color and shape of cotton seed hulls are different from cotton fibers and fabrics, which affect the uniformity of dyeing and the feel and appearance of fabrics. They must be removed before dyeing and finishing of fabrics. However, the structure of cottonseed hulls is dense and the composition is complex, which is the most difficult non-cellulose impurity to remove in the scouring process of cotton fabrics. Compared with other impurities in cotton fiber, its degradation usually requires more severe treatment conditions and longer time, so how to remove cotton seed hulls on the surface of cotton fabric has always been a difficult pro...

Claims

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Application Information

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IPC IPC(8): C12N9/20C12N9/50C12N9/26D06M101/06C12R1/885D06M16/00C12N9/42C12N9/00C12S11/00
Inventor 洪枫唐绿蓉杨雪霞
Owner DONGHUA UNIV
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